11 results about "Bovine hemoglobin" patented technology

The invention provides a folic acid quality control product prepared by using an alternative matrix, and belongs to the technical field of medical detection. The folic acid quality control product is prepared from 5-methyltetrahydrofolic acid, 5, 10-methylenetetrahydrofolic acid and a substitute matrix, the alternative matrix comprises bovine hemoglobin, L-ascorbic acid and a glycine-hydrochloric acid buffer solution (pH 3.0). The alternative matrix can meet the matrix effect requirement and can stabilize the 5-methyltetrahydrofolic acid and the 5, 10-methylenetetrahydrofolic acid for a long time, and the matrix components can be obtained from commercial ways and are low in cost, so that the folic acid quality control product provided by the invention can be widely applied to production and use of folic acid detection reagents or kits, and has wide application prospects. The folic acid detection kit has important significance on market popularization and application of the folic acid detection kit.

This invention relates to the field of biomedical technology, providing a phosphorylated peptide iron chelate of Yanbian cattle hemoglobin, its preparation method, and its applications. The Yanbian cattle hemoglobin phosphorylated peptide iron chelate is prepared from Yanbian cattle blood through enzymatic hydrolysis, phosphorylation, and iron chelation. Specifically, the phosphorylation step actively introduces strongly negatively charged phosphate groups into the Yanbian cattle hemoglobin peptide chain, serving as new and stronger metal ion coordination sites, thereby specifically enhancing its chelating ability for ferrous ions and the stability of the complex. The Yanbian cattle hemoglobin phosphorylated peptide iron chelate possesses high iron content and good gastrointestinal stability, showing broad application prospects in the preparation of a new generation of highly efficient and low-irritant iron supplements.

The invention discloses a functional feed additive for relieving hypoxia stress of dairy cows in a perinatal period, and belongs to the technical field of functional feed additives. The functional feed additive is composed of amino acid chelated iron, a nutrition carrier and water, the iron content is larger than or equal to 9%, and the arginine content is larger than or equal to 10%. The recommended daily supplement dosage is 6.6 g / head, the feed is fed in three times after being mixed with basic ration, and the service cycle is from 21 days before delivery to 21 days after delivery. The additive can significantly improve the hemoglobin concentration and blood oxygen transport capacity of the perinatal dairy cow, relieve hypoxia stress, improve the lactation performance, optimize the metabolic state and enhance the anti-oxidation and anti-inflammatory capacity, is clear in action mechanism, safe and efficient, and is suitable for healthy breeding and production performance improvement of the perinatal dairy cow.

The invention relates to the technical field of medical preparations, in particular to application of a polyethylene glycol bovine hemoglobin conjugate. By constructing a traumatic brain injury rat model, it is found that the polyethylene glycol bovine hemoglobin conjugate can effectively improve learning cognition, memory and exercise capabilities, new application of the polyethylene glycol bovine hemoglobin conjugate is expanded, a new thought is provided for research and development of drugs for improving the learning cognition, memory and exercise capabilities, and the application of the polyethylene glycol bovine hemoglobin conjugate is developed. Wide application prospects are realized.

The present invention provides a folic acid quality control product prepared using an alternative matrix, belonging to the field of medical testing technology. The folic acid quality control product comprises 5-methyltetrahydrofolate, 5,10-methylenetetrahydrofolate, and an alternative matrix; the alternative matrix comprises bovine hemoglobin, L-ascorbic acid, and glycine-hydrochloric acid buffer (pH 3.0). The alternative matrix can not only meet the matrix effect requirements, but also stabilize 5-methyltetrahydrofolate and 5,10-methylenetetrahydrofolate for a long time. In addition, the matrix components can be obtained from commercial channels at a low cost. Therefore, the folic acid quality control product provided by the present invention can be widely used in the production and use of folic acid detection reagents or kits, and is of great significance for the market promotion and application of folic acid detection kits.

The application provides a polyethylene glycolized bovine hemoglobin oxygen carrier and a preparation method and application thereof, relates to the technical field of blood substitutes, and the bovine hemoglobin is modified with methoxypolyethylene glycol vinyl sulfone on the surface. 50 The polyethylene glycolized bovine hemoglobin oxygen carrier has a high P (50% oxygen saturation oxygen partial pressure), can effectively carry and release oxygen, and has a wide application prospect as a blood substitute.

The application discloses a hemoglobin-cobalt phosphate-cobalt phosphate iron hybrid nanoflower and a preparation method and application thereof, and belongs to the technical field of composite materials. The hemoglobin-cobalt phosphate-cobalt phosphate iron hybrid nanoflower is obtained by sequentially adding water-soluble starch, bovine hemoglobin freeze-dried powder, CoSO4 solution, PBS solution, FeSO4 solution and PBS solution into an aqueous solution in an oxygen-free and continuously stirred state through a one-pot method, and then standing and reacting. The synthesis method is simple in process, easy in raw material obtaining, green and environment-friendly, does not need special equipment, and can be prepared in batches. The prepared nanoflower is in the shape of a rose flower, has a large specific surface area and rich electric active centers. The synergistic effect between iron and cobalt ions not only promotes the exchange of electrons between the electric active centers and catalytic substrates, but also improves the intrinsic conductivity of the organic-inorganic hybrid nanoflower, accelerates the electron transmission between the electric active centers and the electrode, and can efficiently catalyze the reduction of hydrogen peroxide.

The invention relates to a carbonic anhydrase purity detection method, and belongs to the technical field of enzyme detection and analysis. The carbonic anhydrase purity detection method provided by the invention comprises the following steps: preparing a to-be-detected sample solution, analyzing by adopting reversed-phase liquid chromatography to obtain a chromatogram, and calculating to obtain the carbonic anhydrase purity, wherein the mobile phases respectively adopt an aqueous solution containing sodium chloride and formic acid and a mixed solution of the aqueous solution containing sodium chloride and formic acid and acetonitrile, and elution separation is carried out under specific elution conditions. By adopting the detection method disclosed by the invention, the purity of carbonic anhydrase in a sample can be rapidly detected within 20 minutes, the result accuracy is high, the precision of the method is good, the durability is good, bovine serum albumin, recombinant carbonic anhydrase and bovine hemoglobin can be well separated, the detection of carbonic anhydrase of various different sources is realized, and the detection method has a wide application prospect. The method has a very high application value in the quality control of carbonic anhydrase products.

The present invention provides a method of treating cancer by administering PEGylated HB-CO (PEG-HB-CO) in combination with radiation therapy. Further, the present invention discloses a method of treating and / or alleviating one or more symptoms of cancer by providing combination therapy to a subject that exhibits improvement by reducing tumor volume and improving the tumor free survival period. The present invention discloses a method of treating cancer by administering PEGylated HB-CO (PEG-HB-CO) simultaneously or sequentially or in any order before or after the radiation therapy.

A kind of multifunctional bovine hemoglobin composite material, preparation method and application thereof belong to the technical field of environmental dye wastewater treatment.The hemoglobin in the blood of slaughtered cattle is used as enzyme-like material, macroporous adsorption resin HPD-400 is used as immobilized carrier, and the hemoglobin and macroporous adsorption resin HPD-400 are immobilized by physical adsorption method through π-π interaction.In the absence of H2O2, the composite material only plays a single adsorption function, and can realize the adsorption and surface enrichment of dyes;in the presence of H2O2, the dye molecules adsorbed by the composite material migrate to the active center of hemoglobin by diffusion, hemoglobin exhibits peroxidase-like activity to oxidize H2O2 to produce free radicals to degrade dye molecules, and the removal efficiency of dyes is improved, showing the dual-function characteristics of adsorption and degradation, and having potential application value in the repair of dye wastewater and other related environmental water bodies.

The invention discloses a polyethylene glycol modified flower-like zinc oxide microsphere as well as a preparation method and application thereof, and belongs to the technical field of targeted protein adsorption separation, and the polyethylene glycol modified flower-like zinc oxide microsphere is prepared by grafting polydopamine to the surface of the flower-like zinc oxide microsphere and carrying out Michael reaction with polyethylene glycol diamine. The flower-like structure of the adsorbent provides a large number of binding sites, and brings about the adsorption capacity of up to 50745.9 mg / g to bovine hemoglobin. In addition, the adsorbent utilizes the unique steric effect of polyethylene glycol diamine to reduce non-specific adsorption to bovine hemoglobin, and shows good adsorption selectivity. The adsorbed bovine hemoglobin is recovered by using 0.5 wt% of lauryl sodium sulfate, and the recovery rate is 87.9%. A circular dichroism map shows that the conformation of bovine hemoglobin is not changed in the adsorption-elution process.