44 results about "Bovine hemoglobin" patented technology

The invention discloses a middle / low concentration positive quality control liquid for urine analysis. The positive quality control liquid comprises a buffer solution with a pH value of 4.5 to 8.5 and a concentration of 20 to 200 mmol / L, a surfactant with the concentration of 20 to 200 mmol / L, a carbohydrate protectant with the concentration of 20 to 200 mmol / L, a protein protectant with the concentration of 20 to 200 mmol / L, anhydrous glucose with the concentration of 300 to 990 mg / L, bovine serum albumin with the concentration of 100 to 1000 mg / L, bovine hemoglobin with the concentration of0.6 to 5 mg / L, esterase with the concentration of 2 to 20 mg / L, acetone or lithium acetoacetate or sodium ethyl acetoacetate or ethyl acetoacetate with the concentration of 50 to 900 mg / L, and a direct bilirubin or naphthylamine salt with the concentration of 3 to 40 mg / L. Quality control objects of corresponding projects can be added as needed. Compared with the prior art, the quality control liquid has the advantages of good stability, high sensitivity, less toxicity of each component, wide source and low production cost.

The invention belongs to the fields of material science and engineering and biological separation engineering, and particularly relates to a quantum dot-based protein imprinted polymer microsphere as well as preparation and application thereof. The CdTe quantum dot is directly used as a functional monomer through taking mercaptoacetic acid (TGA) and glutathione (GSH) together as a stabilizer, protein is a template molecule, dopamine is a cross-linking agent, and the imprinted polymer microsphere is obtained through the auto polymerization of the dopamine. The quantum dot-based protein imprinted polymer microsphere as well as the preparation and the application thereof provided by the invention have the advantages that fluorescence signals of the quantum dots are utilized for detection, and the functionalized quantum dots are directly taken as functional monomers, so that the quantum dots are contained in imprinted holes. Therefore, the detection sensitivity can be improved. Meanwhile, an imprinted polymer layer is obtained through the auto polymerization of the dopamine, and the imprinting process is further simplified. The quantum dot-based protein imprinted polymer microsphere is used for analysis and detection on phycocyanin in a sea water and lake water sample and bovine hemoglobin in a bovine urine sample, the adding standard recovery rate can reach 90 percent or higher, and the reuse performance is good.

The invention specifically relates to a fluorescence carbon spot as well as a preparation method and application thereof and belongs to the field of novel nano material preparation. The fluorescence carbon spot preparation method disclosed by the invention comprises the specific steps: cleaning leaves, putting in an oven to be dried and smashing and screening to obtain leaf powder; adding deionized water into the leaf powder, then adding a polyethyleneimine solution into a magnetic stirring process and continuing magnetically stirring under the room temperature; centrifuging, collecting supernatant and filtering and dialyzing the supernatant to obtain fluorescence carbon spot dispersion liquid. The preparation method disclosed by the invention has simpleness, convenience and safety; meanwhile, use of expensive instruments is reduced; the raw materials are low in cost, environmentally friendly and renewable. The fluorescence carbon spot disclosed by the invention has the advantages of uniform particle size and evenness in dispersion; the fluorescence carbon spot has high detection flexibility to bovine hemoglobin, a linear range is 0 to 54 mu g / L, a complex modified process is avoided, a detection method is simple, and good optical detection capacity is achieved. The fluorescence carbon spot disclosed by the invention has a wide application prospect in detecting bovine hemoglobin.

The invention relates to a method for preparing a bovine hemoglobin / titanium-based titanium dioxide hybrid material and belongs to the technical field of biological-inorganic hybridization. The method comprises the following steps of: preparing a titanium-based titanium dioxide nano array tubular film; and preparing a hemoglobin and titanium-based titanium dioxide nano array tube hybrid film. A highly ordered titanium dioxide nanotube array film is prepared by an anode oxidation method and the hemoglobin is fixed to the titanium-based titanium dioxide nanotube array film by using electrostatic self-assembly technology. The method has the advantages of simple operation and good enzyme activity keeping. The prepared hybrid film can be used in the fields of bio-sensing, biological electrochemical apparatuses and the like.

A process for preparing the poly-haematoglobin with homogeneous molecular weight includes adsorbing haematoglobin by solid phase, adding dual-function reagent, polymerizing reaction, eluting and collecting the polymer by buffering liquid, and chromatography for separating and purifying the polyhaematoglobin. Its advantages are homogeneous molecular weight (about 135 thosands Dol), easy separation, and lower osmotic pressure.

A protein modifier with long tail of monomethoxy polyethanol for modifying human or ox haematoglobin is prepared from the monomethoxy polyethanol with 750-10,000 of mass average molecular weight and L-glutamic acid through condensation reaction.

The preparation method of polymerized hemoglobin with uniform molecular weight involved in the present invention comprises: (1) adsorbing hemoglobin on a stationary phase by a solid-phase adsorption method, and adding a homobifunctional reagent to carry out a polymerization reaction to obtain a polymerized product; (2) The polymer product generated in step (1) is eluted and collected with a high-salt buffer solution without amino groups, and then the collected product is separated and purified by chromatography to obtain polymerized hemoglobin; the hemoglobin used is human hemoglobin, bovine Hemoglobin and porcine hemoglobin; the polymerized hemoglobin prepared by the method is a polymerized hemoglobin (molecular weight is about 135 kilodaltons) of two molecules with uniform molecular weight, which overcomes the inhomogeneous defect of the polymerized product in the traditional glutaraldehyde preparation method; the Polymerized hemoglobin is easily separated from unpolymerized monomeric hemoglobin, and the colloid osmotic pressure is reduced due to the reduction of the number of protein colloids without significant change in viscosity.

The invention discloses a tolyltriazole-containing polyethylene glycol protein modifier, a preparation method thereof and the application thereof, wherein the tolyltriazole-containing polyethylene glycol protein modifier has the following chemical general formula, wherein A is linear-chain alkyl group of which the carbon atom number is 0-20. The preparation method of the modifier comprises the following step of carrying out copper-catalyzed click chemistry on azide group-containing methoxy polyethyleneglycol and terminal acetylene link and succinimide-containing alkane derivative. The tolyltriazole-containing polyethylene glycol protein modifier provided by the invention can be used for modifying the hemoglobin, particularly used for modifying the bovine hemoglobin and the hemoglobin from human blood for human body. According to the method of the copper-catalyzed click chemistry, various functional groups can be effectively modified, and the invention is simple in technology, low in cost, and convenient for industrial production.

The invention discloses molecular imprinted magnetic nanoparticle capable of specifically adsorbing bovine hemoglobin, which belong to the technical field of biochemical separation. The molecular imprinted functionalized magnetic nanoparticles having the capability of specifically adsorbing a target protein can be prepared, the adsorption and separation conditions for bovine hemoglobin are mild bythe nanoparticles, the reaction is simple and rapid, and the specific adsorption of the target protein-bovine hemoglobin can be achieved in a protein mixed solution, which lays a good foundation forthe separation and purification of proteins.

The invention provides a resuscitation fluid applied to the first aid of hemorrhagic shock of pets and a preparation method thereof, belongs to the field of a preparation method of resuscitation fluid, and solves problems that the conventional blood substitutes are single in component, only supply single oxygen and have fewer effects on the expanding of blood volume. The preparation method comprises the following steps: firstly, carrying out a reaction between bovine hemoglobin solution and glutaraldehyde solution, and centrifuging to obtain hemoglobin particles cross-linked with glutaraldehyde; and then, mixing the obtained hemoglobin particles cross-linked with glutaraldehyde with injection solution, to obtain the resuscitation fluid applied to the first aid of hemorrhagic shock of pets, wherein the injection solution is HES, HES2000 or physiological saline. The invention further provides the resuscitation fluid obtained according to the preparation method. The preparation method provided by the invention is simple; the raw materials are easy to obtain; the prepared resuscitation fluid can reversibly bind and release oxygen in vivo and simultaneously expand the blood volume.

The invention belongs to the field of analytical chemistry and quick detection, and particularly relates to a red-green-blue tri-fluorescent emission molecularly imprinted sensor based on a post-imprinting mixing method and a preparation method and application thereof in accurately and visually detecting hemoglobin. The red-green-blue tri-fluorescent emission molecularly imprinted sensor is obtained by mixing red fluorescent bovine hemoglobin imprinted microspheres, green fluorescent bovine hemoglobin imprinted microspheres and blue fluorescent bovine hemoglobin imprinted microspheres throughthe post-imprinting mixing method. The sensor prepared by the method can detect bovine hemoglobin in a high-sensitivity, high-selectivity and self-correcting manner, and the sensor is wider and richerthan conventional dual-fluorescent emission molecularly imprinted sensors in fluorescent color changing range and can visually detect a target more accurately.

The invention relates to an anti-porcine hemoglobin hybridoma cell strain, and a monoclonal antibody and an application of the anti-porcine hemoglobin hybridoma cell strain, and belongs to the technical field of immunology. The anti-porcine hemoglobin hybridoma cell strain PHb-3B4 has a collection number of CCTCC NO (China Center for Type Culture Collection number): C201784. The anti-porcine hemoglobin monoclonal antibody is generated by the anti-porcine hemoglobin hybridoma cell strain; a subtype is IgG1 (immune globulin G1); the valence is 1:819200; an affinity constant is 2.8*10<8>L / mol; and cross reaction rates of the monoclonal antibody with bovine hemoglobin and human hemoglobin are less than 0.1%. The anti-porcine hemoglobin monoclonal antibody is used for detecting porcine hemoglobin, and has the advantages of high valence, good sensitivity and high specificity. The porcine hemoglobin monoclonal antibody can be used for developing an immunological diagnostic reagent related tothe porcine hemoglobin, such as a colloidal gold test strip, a fluorescence detection test strip and an ELISA (enzyme-linked immuno sorbent assay) reagent kit.

The invention relates to a method for preparing a bovine hemoglobin / titanium-based titanium dioxide hybrid material and belongs to the technical field of biological-inorganic hybridization. The method comprises the following steps of: preparing a titanium-based titanium dioxide nano array tubular film; and preparing a hemoglobin and titanium-based titanium dioxide nano array tube hybrid film. A highly ordered titanium dioxide nanotube array film is prepared by an anode oxidation method and the hemoglobin is fixed to the titanium-based titanium dioxide nanotube array film by using electrostatic self-assembly technology. The method has the advantages of simple operation and good enzyme activity keeping. The prepared hybrid film can be used in the fields of bio-sensing, biological electrochemical apparatuses and the like.