11 results about "Blood substitute" patented technology

The subject matter of the present invention is a perfusion fluid in the form of an oil-in-water PFC nanoemulsion, methods of producing this perfusion fluid, and its use in particular for organ perfusion and as a blood substitute preparation.

Compositions comprising Lumbricus terrestris erythrocruorin (LtEc) as an oxygen carrier for use in transfusion and organ perfusion are provided. In particular, methods of using acellular compositions comprising LtEc as a universal blood substitute for transfusion of patients are provided. The methods also include ex vivo perfusion of an organ or tissue with an oxygenated acellular perfusate comprising LtEc as an oxygen carrier. Additionally, methods are provided for using compositions comprising LtEc in treatment of acute ischemia and hyperbaric oxygen therapy.

The invention discloses blood substitute based on bletilla striata polysaccharide and a preparation method thereof, and relates to the technical field of blood substitute. The blood substitute is prepared from the following components in parts by weight: 3 to 5 parts of hydroxyethyl starch, 0.1 to 0.5 part of bletilla striata polysaccharide, 0.1 to 0.5 part of dendrobium officinale polysaccharide, 0.1 to 0.5 part of pullulan, 0.1 to 1 part of sodium chloride, 0.01 to 0.05 part of potassium chloride, 0.01 to 0.05 part of calcium chloride dihydrate, 0.01 to 0.05 part of magnesium chloride hexahydrate, 0.1 to 0.5 part of sodium lactate, 0.05 to 0.1 part of sodium acetate and 100 parts of water for injection. The plasma substitute provided by the invention has an excellent capacity expansion effect.

Provided are a differentiation-inducing culture medium for the transformation of adipose-derived mesenchymal stem cells into chondrocytes and the use thereof. A basal culture medium of the differentiation-inducing culture medium comprises a nutrient solution and an inducer. The nutrient solution comprises a blood substitute, a non-essential amino acid, β-mercaptoethanol, penicillin / streptomycin, dexamethasone, sodium pyruvate, ascorbic acid, sodium selenite, transferrin, recombinant human insulin, β-glycerophosphate and TGF-β1; and the inducer comprises cyanamide, delphinidin chloride and an extracellular matrix derived from adipose-derived mesenchymal stem cells. The components of the inducer, i.e. cyanamide and delphinidin chloride, can stimulate chondrocyte generation by means of up-regulating the expression of aggrecan and collagen II; and the component of the inducer, i.e. extracellular matrix (ADSC-ECM), can increase the adhesion between chondrocytes, enhance the signaling communication between chondrocytes, and promote the growth and metabolism of chondrocytes, thereby greatly improving the differentiation efficiency of adipose-derived mesenchymal stem cells into chondrocytes.

Artificial blood substitutes and methods of using the same are provided. In particular, artificial blood replaces a new enzyme (synthetase) instead of a natural enzyme.

The invention relates to the technical field of biology, in particular to a method for constructing novel artificial erythrocytes based on enucleated mesenchymal stem cells. A novel artificial red blood cell construction method is to be established, hemoglobin alpha and beta subunits are subjected to fusion expression, bone marrow mesenchymal stem cells are taken as a carrier, a stably transfected cell strain capable of stably expressing hemoglobin tetramer is constructed in a lentivirus infection mode, and the stable transfected cell strain has oxygen carrying and oxygen releasing functions. And removing the cell nucleus of the stably transfected cell strain by adopting a centrifugal enucleation method to obtain the artificial red blood cell which is similar to the mature red blood cell in size and has an oxygen transportation function. The artificial red blood cells constructed by the method can be used as a blood substitute to replace red blood cells in blood in a short time to play a role in conveying oxygen under the emergency conditions of insufficient blood reserves or massive hemorrhage and the like at present, so that ischemic symptoms and complications of patients are relieved, and precious rescue time and extra blood source waiting time are won for the patients.

The invention relates to the technical field of cell culture, in particular to a culture medium and a culture method of gamma delta T cells. In order to solve the problems of large batch-to-batch production difference, high biosafety risk, unstable supply chain and high cost caused by combined use of serum or blood substitution and multiple cytokines in the existing gamma delta T cell culture process, the serum-free culture medium containing GlutaMAX and IL-2 is obtained through screening, and the culture medium is applied to gamma delta T cell culture and has the advantages that the culture medium has a wide application prospect. The method can improve the process batch stability, can realize linear amplification, has higher purity and amplification efficiency, reduces the biological safety risk, can significantly reduce the supervision and production cost, and meets the GMP consistency requirement.

The invention discloses a blood transcriptome marker, a kit, a system, a method and application for FDG-PET metabolic load evaluation. The invention provides a blood substitution index of focus FDG-PET inflammation intensity, the substitution index comprises blood transcriptome markers GAS6 and GBP6 and a linear scoring model constructed based on the blood transcriptome markers GAS6 and GBP6, and the substitution index can be used for estimating or reflecting the metabolic load of a focus level in a layering manner and can be used for clinical diagnosis shunt and curative effect follow-up visit. According to the method, a small number of transcripts with the highest positive correlation degree with the focus SUVmax are identified in training data, a linear scoring model is constructed, the focus SUVmax / TGA is mapped and estimated according to the score, and / or whether an active focus exists or not is judged according to a threshold value. The scoring model is strongly and positively correlated with PET image indexes, and can be used as an alternative index of PET metabolic load to assist clinical lesion activity assessment and diagnosis shunting.

Connectors for end-to-end and / or end-to-side coupling of either anatomical tissue to other anatomical tissue and / or non-anatomical devices to anatomical tissue. The connectors are suitable for use in making fluid flow connections, such as allowing blood, blood substitutes, medications, or other fluids to flow through the connector or for medical device access through the connector. The connectors and their variants are each characterized by having at least one mechanically active tine that are actuated by extension or compression of a mechanical mechanism linking the active tines to a frame. The mechanical mechanism may actuate by one or more of shape memory, superelastic, elastic deformation, plastic deformation, electromechanical and / or other motive mechanism operably associated with the at least one mechanically active tines and the frame to rotate the tines about a hinge region under the influence of the mechanical mechanism.

The application relates to the technical field of biological extraction, and discloses a spleen polypeptide extract as well as a preparation method and application thereof. The preparation method takes fresh spleen tissue as raw material, and realizes preliminary separation of proteins, polypeptides and nucleic acids through an optimized isoelectric point grading process after pretreatment, homogenization, repeated freeze-thaw crushing, centrifugal separation and ultrafiltration grading, and finally obtains high-content products by adopting targeted chromatography purification technology. The spleen polypeptide extract and related products can be widely applied to the fields of biological medicines (such as immunomodulators and blood substitutes), food additives (such as protein fortifiers) and scientific reagent fields, the process is mild and has good repeatability, the comprehensive utilization of the spleen raw material is realized, the product added value is improved, and the application prospect of industrialization is good.