3 results about "Collagen ii" patented technology

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The invention relates to the technical field of pet joint protection, and discloses an I-type and II-type collagen composite formula for pet joint health and a preparation method of the I-type and II-type collagen composite formula. The type I and type II collagen composite formula for pet joint health comprises the following components: 15 to 25 parts of type I collagen, 15 to 25 parts of type II collagen, 1 to 3 parts of green lip mussel powder, 35 to 45 parts of chicken hydrolysis powder, 5 to 15 parts of fruit and vegetable products and 5 to 10 parts of a carrier. According to the compound collagen pet food, the action disorder caused by joint degeneration or sport injury can be effectively relieved by utilizing a multi-target synergistic mechanism (such as ligament strengthening, cartilage load reduction and inflammation inhibition of the collagen I and the collagen II in pet joints.

The invention relates to the technical field of preparation of medical stem cell preparations, and discloses a preparation method and a preparation system of a stem cell preparation for repairing joint injury, according to the method, autologous bone marrow MSCs are sorted through microfluidic, SOX9 genes are activated through CRISPR-Cas9 dual targeting, HLA-I / I is knocked out, then a bionic scaffold is subjected to 3D printing based on MRI data, and the stem cell preparation for repairing joint injury is obtained. The method comprises the following steps: fixing stem cells on a stent in a cross-linking manner, loading an exosome through CD63-amino covalent coupling, applying 0.5-1Hz periodic compression load culture according to a viscoelastic model, and performing gradient cryopreservation in a trehalose protective agent; the system comprises five modules: a stem cell pretreatment module, a bionic scaffold construction module, a cell-scaffold compounding module, a dynamic culture module and a cryopreservation quality inspection module. According to the method, the cartilage differentiation efficiency is improved by more than 3 times by utilizing gene editing, the exosome slow release period is prolonged to 14 days, meanwhile, the secretion amount of collagen I is improved by 89% through dynamic culture, and the freezing survival rate is greater than 96%; and the quality inspection qualification rate is 100%.