8 results about "Collagen ii" patented technology

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The invention discloses an antler plate oligopeptide as well as a preparation method and application thereof, and relates to the technical field of bioactive peptides. The preparation method comprises the following steps: extracting an antler plate protein crude extract by taking antler plates as a raw material, preparing short peptides with antler plate molecular weight of less than 3k through pepsin and trypsin hydrolysis, and performing Sephadex G-15 gel chromatography to obtain the antler plate short peptides. The prepared antler plate oligopeptide is low in molecular weight and high in hydrophobicity, easily penetrates through a biological membrane barrier to reach an in-vivo effective part, fully plays the biological activity effect of small molecule peptide, can effectively promote C28 / I2 cartilage cell proliferation, can increase the expression of osteoarthritis related phenotype protein Collagen II in an in-vitro model, can reduce the expression of MMP3, MMP13 and ADAMTS-5, and can be used for treating osteoarthritis. Meanwhile, cartilage matrix degradation in an osteoarthritis rat model is inhibited, cartilage injury is improved, and inflammatory response induced by osteoarthritis is reduced.

The invention discloses application of a phenolic acid derivative or a salt thereof in treatment of osteoarticular degenerative diseases, and particularly relates to a compound shown in the following formula A, the invention also relates to application of the pharmaceutically acceptable salt, the optical isomer, the crystal form, the hydrate, the solvate or the prodrug thereof in preparation of a pharmaceutical composition for treating or preventing degeneration of bone and / or arthritis. The compound provided by the invention can increase the generation of bone tissue collagen I, enhance the osteogenesis of bone tissues, increase the generation of articular cartilage collagen II and delay subchondral bone remodeling, so that the effect of protecting articular cartilage is achieved, and therefore, the compound can be used for preparing medicines for treating and / or preventing bone and joint degenerative diseases. # imgabs0 #

The invention belongs to the technical field of biological medicines, and particularly discloses application of low-concentration recombinant human periostin in preparation of a medicine for treating intervertebral disc degeneration. The invention provides application of low-concentration recombinant human periostin in preparation of a medicine for treating intervertebral disc degeneration. The invention provides application of low-concentration recombinant human periostin in preparation of a medicine for treating intervertebral disc degeneration. The low-concentration recombinant human periostin can remarkably reduce the death rate of intervertebral disc nucleus pulposus cells induced by TNF-alpha, effectively relieve oxidative stress injury, up-regulate the expression of Collagen II and Aggrecan, down-regulate the expression of MMP 13, promote cell proliferation and treat intervertebral disc degeneration.

The invention relates to the technical field of pet joint protection, and discloses an I-type and II-type collagen composite formula for pet joint health and a preparation method of the I-type and II-type collagen composite formula. The type I and type II collagen composite formula for pet joint health comprises the following components: 15 to 25 parts of type I collagen, 15 to 25 parts of type II collagen, 1 to 3 parts of green lip mussel powder, 35 to 45 parts of chicken hydrolysis powder, 5 to 15 parts of fruit and vegetable products and 5 to 10 parts of a carrier. According to the compound collagen pet food, the action disorder caused by joint degeneration or sport injury can be effectively relieved by utilizing a multi-target synergistic mechanism (such as ligament strengthening, cartilage load reduction and inflammation inhibition of the collagen I and the collagen II in pet joints.

The invention relates to the technical field of preparation of medical stem cell preparations, and discloses a preparation method and a preparation system of a stem cell preparation for repairing joint injury, according to the method, autologous bone marrow MSCs are sorted through microfluidic, SOX9 genes are activated through CRISPR-Cas9 dual targeting, HLA-I / I is knocked out, then a bionic scaffold is subjected to 3D printing based on MRI data, and the stem cell preparation for repairing joint injury is obtained. The method comprises the following steps: fixing stem cells on a stent in a cross-linking manner, loading an exosome through CD63-amino covalent coupling, applying 0.5-1Hz periodic compression load culture according to a viscoelastic model, and performing gradient cryopreservation in a trehalose protective agent; the system comprises five modules: a stem cell pretreatment module, a bionic scaffold construction module, a cell-scaffold compounding module, a dynamic culture module and a cryopreservation quality inspection module. According to the method, the cartilage differentiation efficiency is improved by more than 3 times by utilizing gene editing, the exosome slow release period is prolonged to 14 days, meanwhile, the secretion amount of collagen I is improved by 89% through dynamic culture, and the freezing survival rate is greater than 96%; and the quality inspection qualification rate is 100%.

The invention discloses application of STUB1 protein as a BP-3 target spot in osteoarthritis, and relates to the technical field of medicines. The targeted protein STUB1 of the environmental pollution factor BP-3 induced osteoarthritis is screened, and Bp-3 can be strongly combined with the STUB1, so that the occurrence and progress of the osteoarthritis are induced. The STUB1 gene is knocked out and then BP-3 is used for counteracting toxic substances, compared with a group which is not knocked out and only BP-3 counteracting toxic substances, the bone joint injury index MMP13 is reduced, and the human collagen type 2 Collagen II maintaining joint health is increased, which indicates that the knockout of the STUB1 gene or inhibition of STUB1 protein activity in BP-3-induced osteoarthritis is beneficial to relieving cartilage cell injury.

The present invention belongs to the field of biomedicine technology and discloses a stem cell preparation for treating arthritis and a preparation method thereof. The stem cell preparation for treating arthritis of the present invention comprises umbilical cord mesenchymal stem cells pretreated with protocatechuic acid and bilobalide, and a cell protection solution. The umbilical cord mesenchymal stem cells pretreated with protocatechuic acid and bilobalide can effectively inhibit inflammatory responses. The stem cell preparation provided by the present invention can promote the expression of Collagen II and Aggrecan, proteins related to cartilage repair in mice, and is suitable for alleviating inflammation and repairing cartilage tissue in arthritis diseases, thereby achieving symptom relief for patients.