259 results about "Protein activity" patented technology

The invention discloses a protein active site multi-classification identification method based on multi-modal deep learning. According to the method, protein sequence information, three-dimensional structure information and functional text information are fused, a pre-trained protein language model, an isotropic graph neural network and a biomedical language model are utilized, an innovative multi-modal feature extraction and fusion mechanism is designed, and the model performance is optimized through a self-adaptive weighted fusion strategy. According to the method provided by the invention, accurate prediction of protein active sites can be realized through acquisition of a high-quality data set, construction of a cross-modal feature fusion module, setting of a weighted fusion mechanism and design of a composite loss function.

Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity of RNA-programmable endonucleases, such as Cas9, or for controlling the activity of proteins comprising a Cas9 variant fused to a functional effector domain, such as a nuclease, nickase, recombinase, deaminase, transcriptional activator, transcriptional repressor, or epigenetic modifying domain. For example, the inventive proteins provided comprise a ligand-dependent intein, the presence of which inhibits one or more activities of the protein (e.g., gRNA binding, enzymatic activity, target DNA binding). The binding of a ligand to the intein results in self-excision of the intein, restoring the activity of the protein.

The invention relates to the technical field of nucleic acid detection, in particular to a method for regulating and controlling CRISPR-Cas protein activity through temperature and application of the method, a CRISPR reaction system contains Cas protein, guide RNA, a nucleic acid reporter probe and a buffer solution, and then the cis-cleavage activity or trans-cleavage activity of the Cas protein is activated or inactivated by regulating and controlling the temperature of the reaction system; the guide RNA includes a guide RNA targeting a target nucleic acid sequence that does not contain a PAM site. Multiple detection can be realized only by using the same type of Cas protein, the design of PAM-free sgRNA or crRNA facilitates the selection of sgRNA or crRNA, meanwhile, the activity of PAM-free sgRNA or crRNA can be regulated and controlled by utilizing temperature rise, and the Cas protein with lower reaction temperature can be inactivated, so that the Cas protein cannot cause interference to the detection of the second stage.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention discloses an application of an OsSPL14 gene and a protein coded by the OsSPL14 gene in regulating and controlling the diameter of a plant root system, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the root diameter of a plant. In order to solve the technical problem, the invention provides application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the diameter of a plant root system. The protein has an amino acid sequence of sequence 2. The invention discloses that OsSPL14 is an important gene for regulating and controlling the diameter of the rice root for the first time. The expression of the OsSPL14 is driven by using a specific promoter, so that the regulation effect of the OsSPL14 in a rice root system can be specifically exerted, and the diameter of the adventitious root of the rice is increased. The OsSPL14 provided by the invention has important significance in cultivating a new rice variety with high and stable yield, high quality and good stress resistance.

The invention belongs to the technical field of biomedicine, and particularly relates to application of GPR161 as an acute distress syndrome diagnostic marker and a therapeutic target. Experiments prove that the expression level of GPR161 in peripheral blood mononuclear cells of a patient with the acute respiratory distress syndrome is remarkably increased, and the expression quantity of the GPR161 is positively correlated with the severity of the disease, so that the GPR161 gene or GPR161 protein can be used as a molecular marker for screening or diagnosis or prognosis evaluation of the acute respiratory distress syndrome; a reagent for inhibiting GPR161 gene expression or protein activity can be used for preparing a medicine for treating the acute respiratory distress syndrome. The invention provides a new strategy for diagnosis, monitoring and targeted drug development of ARDS, and has important clinical application value.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.

The invention provides application of an FAP protein function inhibitor in preparation of a medicine for inhibiting formation of fibrous scars. The invention finds that the formation of the fibrous scar can be obviously inhibited by knocking out the Fap gene and inhibiting the activity of the FAP protein, so that the FAP protein function inhibitor can be used for preparing the medicine for inhibiting the formation of the fibrous scar. Besides, the invention also finds that the expression levels of the Fap gene and the FAP protein are also increased along with the development of the fibroscar disease course, which indicates that the FAP can be used as a marker of the fibroscar, that is, the FAP can be used as a target for screening products for inhibiting the formation of the fibroscar, and a detection reagent of the FAP can be used for preparing fibroscar diagnosis products and / or prognosis evaluation products.

The present invention relates to the preparation and application of biological peptides, and in particular to a mussel peptide and its application in the preparation of uric acid-lowering products. The amino acid sequence is shown in SEQ ID NO.1‑4. The present invention prepares mussel polypeptides by composite enzyme-directed enzymatic hydrolysis and membrane separation and purification technology, and screens out four novel polypeptides with potential biological activity by combining LC‑MS / MS analysis and activity prediction. Molecular docking and cell experiments have confirmed that these four polypeptides can specifically inhibit GLUT9 protein activity and reduce uric acid levels; animal experiments have further verified their efficacy in lowering uric acid, providing a scientific basis for the high-value development of mussel resources and the development of functional uric acid-lowering products.

The invention discloses application of ZmCOP1a and ZmCOP1b proteins and coding genes of the ZmCOP1a and ZmCOP1b proteins in regulation and control of plant yield. The invention belongs to the technical field of biology, and particularly relates to application of ZmCOP1a and ZmCOP1b proteins and coding genes thereof in regulation and control of plant yield. The proteins ZmCOP1a and ZmCOP1b or expression substances for regulating genes or substances for regulating the activity or content of the proteins can be applied to any one of the following applications: U1) application in regulating the plant yield; the invention also relates to application of the U3) in preparation of products for regulating and controlling plant yield, application of the U3) in preparation of products for regulating and controlling plant yield, application of the U4) in preparation of products for cultivating plants with changed plant yield, and application of the U5) in plant breeding.

The present invention relates to compositions and methods for subpopulation-specific modulation of cell function and for treating repeat expansion disorders comprising genetic, degenerative, neurological and cellular diseases, including immune disorders. Also provided are research kits for subpopulation-specific modulation of protein activity and the corresponding potential to discover novel therapeutic compositions. More specifically, the disclosed compositions and methods selectively up- or downregulate at least a first population of a cellular protein or therapeutic target with minimal or negligible effect on the activity of at least a second population of the cellular protein or therapeutic target.

The invention relates to the technical field of biology, in particular to application of rice LOCOs06g20960 protein to regulation and control of plant disease resistance. The invention discloses application of a substance for protecting the protein or regulating the expression substance of the coding gene of the protein or regulating the activity or content of the protein in any one of Y1-Y5: application of Y1 in regulating the disease resistance of plants; the Y2 is applied to preparation of a product for regulating and controlling plant disease resistance; the Y3 is applied to cultivation of plants with changed disease resistance; the Y4 is applied to preparation of products for cultivating plants with changed disease resistance; the invention discloses application of Y5 in plant breeding. It is found that the rice LOCOs06g20960 protein has the function of positively regulating and controlling the bacterial blight resistance of rice, the bacterial blight resistance of the rice can be remarkably improved by transferring the LOCOs06g20960 gene overexpression sequence into the rice, and the rice LOCOs06g20960 gene overexpression sequence is of great significance to cultivation of new bacterial blight resistant rice varieties.

The invention belongs to the technical field of biological medicine, and particularly provides application of a PLA1A gene / protein as an anti-tumor target. The key effect of the PLA1A gene / protein in promoting chemotherapy drug resistance is disclosed for the first time, and a new mechanism that the PLA1A gene / protein affects chemotherapy sensitivity by regulating a tumor microenvironment (especially macrophage polarization) is deeply clarified. Researches prove that knockdown of the PLA1A gene or inhibition of the protein activity of the PLA1A gene can significantly enhance the sensitivity of tumor cells to chemotherapeutic drugs (such as oxaliplatin), so that the therapeutic effect is improved, and the sensitization provides a new strategy for overcoming tumor chemotherapeutic drug resistance. In animal model experiments, knockdown of the PLA1A is combined with oxaliplatin for treatment, so that growth of mouse transplanted tumors and liver metastases is remarkably inhibited, the survival time of tumor-bearing mice is prolonged, and it is indicated that the PLA1A has important clinical application value and wide application prospects as an anti-tumor target.

The invention relates to the technical field of biological medicines, and discloses application of INHBC as a target spot in prevention and / or treatment of metabolic diseases. Specifically, the invention relates to application of substances for promoting INHBC gene expression or improving protein activity in preparation of products for preventing and / or treating metabolic diseases, and the metabolic diseases comprise diseases caused by energy metabolism disorder, such as obesity, diabetes, fatty liver disease, hyperlipidemia and the like. According to the application disclosed by the invention, liver specific overexpression or INHBC knock-in can improve mouse liver fatty degeneration and abnormal glucose metabolism induced by high fat diet, and in addition, the heat production activity of mouse adipose tissues can be enhanced, so that obesity induced by high fat diet is inhibited; the compound has an important application value in preventing and / or treating metabolic diseases such as obesity, type 2 diabetes and fatty liver diseases.

The invention discloses application of a substance for inhibiting MED10 protein activity and / or expression quantity in treatment of liver cancer. Experiments prove that the expression quantity of the MED10 protein and the encoding gene thereof in liver tissues or plasma of liver cancer patients is obviously higher than that of healthy people; by inhibiting the activity and / or expression quantity of the MED10 protein, proliferation of liver cancer cells can be inhibited, growth of the liver cancer cells can be inhibited, apoptosis of the liver cancer cells can be promoted, diffusion of the liver cancer cells can be inhibited, and metastasis of the liver cancer cells can be inhibited, so that the liver cancer can be treated. The method has an important application value.

This invention discloses a method for preparing protein powder through low-temperature separation, comprising the following steps: S1: raw material pretreatment; S2: low-temperature defatting; S3: multi-stage low-temperature membrane separation; S4: low-temperature concentration; S5: freeze-drying; S6: post-treatment. This invention uses animal-derived whey as raw material, sequentially performing raw material pretreatment, low-temperature defatting, multi-stage low-temperature membrane separation, low-temperature concentration, freeze-drying, and post-treatment. The temperature is controlled within the range of 0–10°C throughout the process. By optimizing the pretreatment process to remove impurities and heat-sensitive interfering substances, and employing a combination of gradient-pore-size ceramic membranes and ultrafiltration membranes, efficient separation and purification of proteins are achieved. Combined with vacuum low-temperature concentration and freeze-drying technology, the active components of whey protein are preserved to the maximum extent. This invention solves the problems of large protein activity loss, low purity, high energy consumption, and easy introduction of chemical contaminants in existing preparation methods.

The present invention provides a universal protein renaturation method and an application thereof, and relates to the technical field of protein renaturation. The method includes the following steps: (1) add 2mercaptoethanol to urea solution to obtain denaturing solution; (2) dissolve a sample including to-be-renatured protein by using the denaturing solution and then freeze the sample, to obtain a frozen body; (3) thaw the frozen body at room temperature, and extract the thawed liquid as soon as the liquid is thawed; (4) dilute the extracted liquid with water, to terminate renaturation and obtain supernatant and precipitate through centrifugation, where the supernatant includes the protein that has been successfully renatured. According to the present invention, protein renaturation recovery efficiency can be improved, and a production batch is stable and reproducible. Operations in the present invention are simple, almost no waste liquid is produced, and no protein is lost. According to the present invention, nine allergen proteins are renatured, to significantly improve the protein renaturation yield. In addition, compared with that in the conventional technology, the protein activity according to the present invention is increased by about 3 times or more.

The application discloses application of ZmSAUR72 protein and a coding gene thereof in regulation of drought resistance of plants and belongs to the technical field of genetic engineering breeding. The application discloses application of the ZmSAUR72 protein or an expression material for regulating the coding gene of the protein or a material for regulating the activity or content of the protein in regulation of drought resistance of plants. The amino acid sequence of the protein is shown as SEQ ID No. 1, the nucleotide sequence of the coding gene of the protein is shown as SEQ ID No. 1, and it is verified that the regulation in the above application is positive regulation. By increasing the expression amount and / or activity of the ZmSAUR72 protein, the interval of corn loose spinning time is reduced, the drought resistance of plants is enhanced, the grain number of a single ear of plants is increased, and the yield of a single ear of plants is increased. The negative regulation effect of inhibition or reduction of the expression amount of the ZmSAUR72 gene in the above application is also verified by a gene knockout method. The application further discloses a method for improving the drought resistance of plants, which comprises introducing the coding gene of the protein into a receptor plant to obtain a plant with improved drought resistance.

The invention discloses application of ACSS2 as an action target in screening or preparing a medicine for preventing or treating virus-induced vascular remodeling diseases, and belongs to the field of biological medicine. It is found that by inhibiting ACSS2 gene expression or protein activity, Zika virus induced vascular smooth muscle cell phenotypic transformation can be remarkably reversed, and occurrence and development of angiotensin II / aminopropionitrile induced aortic aneurysm / dissection are relieved. The invention provides a siRNA sequence (SEQ ID NO.1-2) specifically targeting ACSS2 and a pharmaceutical composition containing the siRNA, and provides a novel treatment target and a precise intervention tool for virus-related vascular remodeling diseases.

The invention discloses a method for improving botrytis cinerea resistance of plants, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the expression of a coding gene of protein in a receptor plant or up-regulating or enhancing or improving the activity and / or content of the protein, so that the resistance of the receptor plant to gray mold is increased, and the amino acid sequence of the protein is SEQ ID NO: 1. Experiments prove that the disease resistance of plants to gray mold can be improved by utilizing the protein SPR7 and the coding gene thereof provided by the invention, and compared with Ailsa Craig plants, the resistance of T1-generation SPR7 transgenic tomato plants to gray mold is greatly improved. Therefore, the protein SPR7 and the coding gene thereof have great theoretical significance and practical value in improving the botrytis cinerea resistance of plants. The invention has important application and market prospects in the field of agriculture.

The invention discloses a compound for targeted ubiquitination degradation of USP7 protein, and a medicinal composition and application thereof. The structural formula of the E3 ubiquitin ligase complex is as shown in formula I. A is a specific protein ligand in the E3 ubiquitin ligase complex; l is a bivalent linking group between a USP7 protein small molecule ligand and a specific protein ligand in an E3 ubiquitin ligase complex. The protein degradation chimera has the activity of inhibiting USP7 protein and the activity of degrading USP7 protein, and can effectively inhibit malignant proliferation of acute lymphatic leukemia cells, so that the protein degradation chimera can be used for related diseases with abnormal expression of USP7 protein.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The utility model relates to the technical field of protein purification, and particularly discloses a portable low-temperature protein purification box which comprises a box body, a control panel is mounted at the top of the box body, a temperature sensor is mounted on the inner wall of the box body, and a sample loading module, a purification module, a detection module and a sample collection module are arranged in the box body. The sample loading module comprises a sample liquid storage device, a buffer liquid storage device and a peristaltic pump which are mounted in the box body, the purification module comprises a chromatographic column connected with the peristaltic pump, and the detection module comprises an ultraviolet detector mounted in the box body. Through the design of the low-temperature control system and the heat preservation layer, protein purification can be carried out in a low-temperature environment, the activity of the protein is effectively maintained, and the problem that the protein is inactivated or even degraded due to the fact that the temperature is too high in a traditional purification method is solved; meanwhile, the influence of long-time low-temperature experiments on the body health of experiment operators is avoided.

This invention relates to an antibacterial membrane-penetrating peptide and its applications. The amino acid sequence of the antibacterial membrane-penetrating peptide includes the sequence shown in SEQ ID NO.1 or a sequence with more than 70% homology to the sequence shown in SEQ ID NO.1. This invention screened and obtained a short peptide that specifically binds to the bacterial Hsp70s protein. It was found that this peptide binds to the Hsp70s protein through a traditional substrate-binding pocket, exhibiting an unusually high affinity for the protein in the DnaK-ATP state. The short peptide can inhibit Hsp70s protein activity to a certain extent, preventing it from aiding in the renaturation of denatured luciferase. Furthermore, while maintaining the integrity of the bacterial cell membrane, this short peptide has a superior ability to cross the bacterial cell membrane. The invention further discusses its application potential in the preparation of antibacterial drugs and antibiotic adjuvants, providing new ideas for solving current problems such as bacterial resistance and the difficulty in discovering new antibiotics.

The present invention relates to a gene variant of the transcriptional regulator LysG and a method for producing L-citrulline or L-arginine using same. The gene variant of the transcriptional regulator LysG according to the present invention has protein activity that is altered due to the mutation of one or more bases in the base sequence of a gene encoding the transcriptional regulator LysG, and thus, it is possible to effectively produce L-citrulline or L-arginine from a recombinant microorganism comprising the variant.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.

A protein active site prediction method based on geometric graph neural network belongs to the field of bioinformatics and protein structure analysis. First, the original protein data is preprocessed by multi-modal feature extraction and geometric graph construction, and ProtT5 deep embedding and physicochemical properties are fused. Then, a deep ActiveSiteGNN model with explicit geometric perception ability is constructed, and the stacked geometric encoder and geometric edge update layer are used to dynamically capture the micro three-dimensional spatial features. Next, a multi-task collaborative optimization and dynamic threshold search strategy is designed, combined with weighted sampling to solve the serious sample imbalance, and the best decision threshold is selected based on the validation set in real time. The integrated reasoning and graph diffusion smoothing technology is introduced to globally calibrate the prediction probability distribution based on the biological space prior. Finally, the evaluation is carried out on the independent test set. The method has strong structure perception ability and provides a feasible solution for accurate prediction of protein functional sites.

Provided is a method for producing, by culturing, a cell for measuring the activity of a sensor protein that can be used as a chemical substance sensor. A method for producing a cell for measuring the activity of a sensor protein, the method comprising: culturing a cell containing a polynucleotide containing a sensor protein coding sequence, and when the ratio (specific activity) of the activity value of the sensor protein when the concentration of a response substance is 1 [mu] M to the activity value when the concentration of the response substance is 0 [mu] M is 1.2 or more; and recovering the cells.

The application relates to the field of biological medicine technology and discloses normal-temperature preservation of stem cell exosomes in skin-care composition application, which comprises the following steps: obtaining a secondary drying time according to sample temperature and shelf temperature; obtaining a moisture step degree according to the difference of residual moisture content of a pressure increasing rate; obtaining a moisture continuous removal index according to the residual moisture content, combining the moisture step degree to obtain a comprehensive moisture removal efficiency; obtaining an activity loss degree according to protein activity and the comprehensive moisture removal efficiency, and then obtaining a water content critical threshold value to construct a damage sensitivity; obtaining a damage index according to the protein activity, the comprehensive moisture removal efficiency and the damage sensitivity, and selecting a target pressure increasing rate; and performing stem cell exosome production application in skin-care composition according to the secondary drying time, the water content critical threshold value and the target pressure increasing rate. The application prolongs the normal-temperature preservation duration of stem cell exosomes in skin-care composition application by regulating the secondary drying process.