441 results about "Protein activity" patented technology

The invention discloses a protein active site multi-classification identification method based on multi-modal deep learning. According to the method, protein sequence information, three-dimensional structure information and functional text information are fused, a pre-trained protein language model, an isotropic graph neural network and a biomedical language model are utilized, an innovative multi-modal feature extraction and fusion mechanism is designed, and the model performance is optimized through a self-adaptive weighted fusion strategy. According to the method provided by the invention, accurate prediction of protein active sites can be realized through acquisition of a high-quality data set, construction of a cross-modal feature fusion module, setting of a weighted fusion mechanism and design of a composite loss function.

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

The present invention relates to a novel heterocyclic compound, which is a compound having FGFR3 protein activity. Specifically, the invention relates to a compound as shown in formula (I), or pharmaceutically acceptable salts, solvates, polymorphic substances or isomers thereof, and application of the compound in preparation of medicines for treating diseases related to FGFR3 (Fibroblast Growth Factor Receptor 3). # imgabs0 #

Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity of RNA-programmable endonucleases, such as Cas9, or for controlling the activity of proteins comprising a Cas9 variant fused to a functional effector domain, such as a nuclease, nickase, recombinase, deaminase, transcriptional activator, transcriptional repressor, or epigenetic modifying domain. For example, the inventive proteins provided comprise a ligand-dependent intein, the presence of which inhibits one or more activities of the protein (e.g., gRNA binding, enzymatic activity, target DNA binding). The binding of a ligand to the intein results in self-excision of the intein, restoring the activity of the protein.

The present disclosure relates to compounds of formula (I) and their anti-tumor uses, and their intermediates of formula (III), intermediates of formula (IV), and uses of the intermediates. The compound of formula (I) has a degrading effect on a specific target protein, which is mainly composed of three parts. The first part is a small molecule compound (SMBP, Small Molecules Binding Protein) that can bind to a protein, the second part LIN is a linker, and the three-part ULM is a ubiquitin ligand (ULM, Ubiquitin Ligase Binding Moiety), wherein SMBP is covalently bound to LIN, and LIN is covalently bound to ULM. A series of compounds designed and synthesized in the present disclosure have a wide range of pharmacological activities, including the functions of degrading specific proteins and / or inhibiting activities of specific proteins, and thus can be used in related tumor treatments.

The invention belongs to the technical field of medical treatment, and discloses a blood product virus inactivation system based on a synergistic effect of nano-catalysis photochemical inactivation and low-temperature plasma, the system comprises: a nano-photocatalysis inactivation module adopting titanium dioxide TiO2 and graphite phase carbon nitride g-C3N4 nano-materials as photocatalysts; the low-temperature plasma inactivation module adopts a low-temperature plasma generator to generate plasma cloud above the blood product and release active particles; the dynamic fluid stirring module is used for enabling the blood product to uniformly receive photocatalysis and plasma inactivation effects through a microfluid mixing technology so as to ensure the uniformity of virus inactivation; the sensing monitoring and intelligent feedback module is used for monitoring the residual quantity of viral nucleic acid and the activity of protein in the blood in real time by adopting a spectrum sensor and a biochemical sensor. The method can be widely applied to the fields of clinical blood transfusion, blood product manufacturing, blood disease treatment and the like, promotes the progress of a blood safety guarantee technology, reduces the virus infection risk and improves the patient safety.

The invention relates to the technical field of nucleic acid detection, in particular to a method for regulating and controlling CRISPR-Cas protein activity through temperature and application of the method, a CRISPR reaction system contains Cas protein, guide RNA, a nucleic acid reporter probe and a buffer solution, and then the cis-cleavage activity or trans-cleavage activity of the Cas protein is activated or inactivated by regulating and controlling the temperature of the reaction system; the guide RNA includes a guide RNA targeting a target nucleic acid sequence that does not contain a PAM site. Multiple detection can be realized only by using the same type of Cas protein, the design of PAM-free sgRNA or crRNA facilitates the selection of sgRNA or crRNA, meanwhile, the activity of PAM-free sgRNA or crRNA can be regulated and controlled by utilizing temperature rise, and the Cas protein with lower reaction temperature can be inactivated, so that the Cas protein cannot cause interference to the detection of the second stage.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The present disclosure provides methods and compositions relating to inhibiting the activity of an epithelial modulatory protein in a cell, and reversing or preventing changes in a cell associated with a disease or condition, such as a fibrotic disease or condition, including the use of an inhibitor of epithelial modulatory protein activity. Provided herein are isolated antibodies, or antigen-binding fragments thereof, that specifically bind to epithelial regulatory proteins as exemplary inhibitors of epithelial regulatory protein activity. Also disclosed are methods and compositions useful for treating or preventing a fibrotic disease or condition in a subject, e.g., including an inhibitor of epithelial regulatory protein activity. The present disclosure also includes kits comprising compositions useful in performing the disclosed methods.

The invention discloses a construction method and application of a gastric adenocarcinoma transgenic mouse model, and the construction method comprises the following steps: overexpressing or increasing the MYC gene expression quantity or MYC protein activity in gastric mucosa epithelial cells of a mouse by using transgenosis and hybridization technologies, and activating KRAS gene mutation in the gastric mucosa epithelial cells at the same time, thereby obtaining the gastric adenocarcinoma transgenic mouse model. The Kras protein is abnormal in function and is continuously in an activated state, and the finally obtained mouse is the gastric adenocarcinoma transgenic mouse model. According to the invention, the Myc gene overexpression and KRAS activation mutation double-gene mutation mouse spontaneous gastric cancer animal model is constructed, and the method is simpler. The gastric cancer tumor formation time of the mouse model is shortened, the tumor formation rate is up to 100%, and the tumor formation effect is stable. Only tumor formation is performed in the stomach through induction, and tumor formation has high specificity. The animal model is determined to be adenocarcinoma in gastric cancer through pathological section and immunohistochemical staining, has good clinical representativeness, and can be applied to later development or screening of gastric cancer drugs.

The invention discloses an application of a small molecule compound SFII in preparation of drugs for resisting pyroptosis and GSDMD inhibitors with pyroptosis resisting activity. The small molecule compound Scutellaria flavone II (SII) extracted from Scutellaria baicalensis roots plays an important role in preparation of drugs for inhibiting pyroptosis inflammation so as to prevent or treat atherosclerosis. Experiments prove that the active pharmaceutical ingredient SFII has a definite effect on promoting atherosclerosis by virtue of Gasdermin D and GSDMD mediated macrophage pyroptosis, is convenient to obtain, can be used for preparing drugs for inhibiting GSDMD protein activity to resist pyroptosis and exerting an anti-inflammatory effect so as to prevent or treat atherosclerosis, and has a remarkable effect. The small molecule compound SFII provides a new treatment strategy for atherosclerosis, and has a wide application prospect.

The invention discloses an application of osmundone in preparation of a medicine for treating methicillin-resistant staphylococcus aureus infectious pneumonia. Experimental results of an MRSA mouse pneumonia infection model show that osmundone can significantly reduce the bacterial load in lung tissues, alleviate excessive inflammatory response caused by infection and effectively improve the survival rate of mice. In addition, the invention discloses that osmundone reduces the adhesion and invasion ability of bacteria, reduces the toxicity of the bacteria and plays an antibacterial role by inhibiting the protein activity of staphylococcus aureus Sortase A (SrtA). Different from traditional antibiotics, osmundone does not apply survival pressure to bacteria, so that generation of drug resistance is not easily induced. On the basis, osmundone shows a good application prospect in the aspect of research and development of safe and effective MRSA infectious pneumonia treatment drugs.

The invention discloses an application of a sulfanyl acetamide compound with a structure shown in a formula I in preparation of a medicine for preventing and / or treating tumors and idiopathic pulmonary fibrosis. The compound can be stably combined with key amino acid residues of DDR1 protein, has a remarkable inhibition effect on phosphorylation activation of the DDR1 protein and various tumor cells, plays an anti-tumor role by effectively inhibiting protein activity, and provides an effective lead compound for subsequent drug design. # imgabs0 #

The invention discloses application of a CMIET protein and a coding gene thereof in improving manganese resistance of plants. The invention belongs to the technical field of biology, and particularly relates to application of CMIET protein and a coding gene thereof to improvement of manganese resistance of plants. The CMIET protein can regulate and control biological manganese resistance, and excessive manganese in Chlamydomonas chloroplast can be discharged to cytoplasm by regulating and controlling expression of the protein CMIET or regulating and controlling gene or regulating and controlling activity or content of the protein CMIET, so that balance of manganese ion concentration in the chloroplast is maintained, and the chloroplast is prevented from being affected by manganese toxicity; the normal photosynthesis capability of the cells is maintained, and the normal photosynthesis is ensured.

The invention discloses application of an MsERF07 gene in regulation and control of drought resistance of plants, and is characterized in that a precursor sequence of the MsERF07 gene is as shown in SEQ ID No.1, and a mature sequence of the MsERF07 gene is as shown in SEQ ID No.2. The invention further discloses application of the MsERF07 gene in regulation and control of drought resistance of plants. The gene or an expression substance for regulating and controlling the gene or a substance for regulating and controlling the activity or content of protein can be applied to the aspect of regulating and controlling the drought resistance of plants, the drought resistance of the plants can be improved by over-expressing the MsERF07 gene, and the gene has a positive effect on accelerating and improving the variety of medicago sativa.

The invention relates to the technical field of biology, in particular to a tri-specific antibody based on structure optimization protein activity and application of the antibody. Specifically, the invention relates to a trispecific antibody specifically combined with Her2, CD3 and CD28, and the Her2 / CD3 / CD28 trispecific antibody comprises a Fab part of a monoclonal antibody specifically combined with CD3, an antibody fragment specifically combined with Her2 and an antibody fragment specifically recognized with CD28. The invention also relates to an application of the antibody in preparing a medicine for treating cancers, and the antibody plays an important role in treating human tumors.

The invention discloses an application of an OsSPL14 gene and a protein coded by the OsSPL14 gene in regulating and controlling the diameter of a plant root system, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the root diameter of a plant. In order to solve the technical problem, the invention provides application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the diameter of a plant root system. The protein has an amino acid sequence of sequence 2. The invention discloses that OsSPL14 is an important gene for regulating and controlling the diameter of the rice root for the first time. The expression of the OsSPL14 is driven by using a specific promoter, so that the regulation effect of the OsSPL14 in a rice root system can be specifically exerted, and the diameter of the adventitious root of the rice is increased. The OsSPL14 provided by the invention has important significance in cultivating a new rice variety with high and stable yield, high quality and good stress resistance.

The invention belongs to the technical field of biomedicine, and particularly relates to application of GPR161 as an acute distress syndrome diagnostic marker and a therapeutic target. Experiments prove that the expression level of GPR161 in peripheral blood mononuclear cells of a patient with the acute respiratory distress syndrome is remarkably increased, and the expression quantity of the GPR161 is positively correlated with the severity of the disease, so that the GPR161 gene or GPR161 protein can be used as a molecular marker for screening or diagnosis or prognosis evaluation of the acute respiratory distress syndrome; a reagent for inhibiting GPR161 gene expression or protein activity can be used for preparing a medicine for treating the acute respiratory distress syndrome. The invention provides a new strategy for diagnosis, monitoring and targeted drug development of ARDS, and has important clinical application value.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.

The invention provides application of an FAP protein function inhibitor in preparation of a medicine for inhibiting formation of fibrous scars. The invention finds that the formation of the fibrous scar can be obviously inhibited by knocking out the Fap gene and inhibiting the activity of the FAP protein, so that the FAP protein function inhibitor can be used for preparing the medicine for inhibiting the formation of the fibrous scar. Besides, the invention also finds that the expression levels of the Fap gene and the FAP protein are also increased along with the development of the fibroscar disease course, which indicates that the FAP can be used as a marker of the fibroscar, that is, the FAP can be used as a target for screening products for inhibiting the formation of the fibroscar, and a detection reagent of the FAP can be used for preparing fibroscar diagnosis products and / or prognosis evaluation products.

The present disclosure provides oligonucleotides comprising at least one radical of a central nervous system receptor ligand at a nucleobase, the 2' position of a nucleoside, or the 3' position of a nucleoside. The present disclosure also provides pharmaceutical compositions and kits comprising said oligonucleotides, methods of delivering said oligonucleotides, methods of modulating the activity of a protein using such oligonucleotides, and methods of treating, preventing, or diagnosing diseases (e.g., central nervous system diseases, neurodegenerative diseases, and neurocognitive diseases) in a. subject in need thereof using said oligonucleotides or pharmaceutical compositions thereof. The present disclosure also provides oligonucleosides that may be useful for making the oligonucleotides.

An initial protein sequence is identified and varied to generate a plurality of variants. An activity of the plurality of protein sequences is measured quantitatively. Each of the plurality of protein sequences is provided as an input to a large language model (LLM) to generate a corresponding plurality of embeddings in a latent space. A subset of the plurality of embeddings is used to train a top layer model. The plurality of embeddings are provided as inputs to the top layer model to generate outputs representing predictions of the activity of the plurality of protein sequences. A subset of the plurality of protein sequences is selected based on the top layer model outputs. The method repeats, with the selected subset of the plurality of protein sequences playing the role of the initial protein sequence in the initial iteration, until some termination criterion is satisfied.

The invention discloses a purification method of bovine serum albumin. The purification method comprises the following steps: S1, pre-treating bovine plasma to remove impurities and fibrin; s2, adsorption with tourmaline or sepiolite; s3, protein precipitation through heat shock; and S4, carrying out heparin-agarose chromatography. The bovine serum albumin sample is purified by combining tourmaline or sepiolite with heparin-agarose chromatography, nuclease in the bovine serum albumin sample can be removed, and the product purity is improved; and one-step heat shock is combined with heparin-agarose chromatography, so that the energy consumption is reduced, reagents such as ethanol influencing the protein activity are prevented from being used, the recovery yield is high, and large-scale production is facilitated.

The invention discloses application of Msa-miR398a-3p in regulation and control of drought resistance of plants, and is characterized in that a precursor sequence of the Msa-miR398a-3p is as shown in SEQ ID No. 1, and a mature sequence of the Msa-miR398a-3p is as shown in SEQ ID No. 2; the gene or an expression substance for regulating and controlling the gene or a substance for regulating and controlling the activity or content of protein can be applied to the aspect of regulating and controlling the drought resistance of plants, the drought resistance of the plants can be improved by silently expressing Msa-miR398a-3p, and the gene has a positive effect on accelerating and improving the variety of medicago sativa.

The invention discloses application of rice OsC3HC4 protein and a coding gene thereof in regulating and controlling high temperature resistance of rice. The invention belongs to the field of plant breeding, and particularly relates to application of rice OsC3HC4 protein and a coding gene thereof to regulation and control of high temperature resistance of rice. The protein or the substance for regulating and controlling the activity or content of the protein or regulating and controlling the expression of the coding gene of the protein can be applied to the following aspects: 1) regulating and controlling the high-temperature resistance of plants; 2) preparing a product for regulating and controlling the high temperature resistance of plants; 3) cultivating plants with high-temperature resistance change; 4) preparing a product for cultivating the plant with the high temperature resistance changed; and 5) plant breeding. After the coding gene of the OsC3HC4 protein is subjected to CRISPR gene editing and knockout, an OsC3HC4 knockout mutant after high-temperature stress treatment has a high-temperature-resistant phenotype, which indicates that the OsC3HC4 gene plays an important role in regulating and controlling the high-temperature resistance of rice.

The present invention relates to the preparation and application of biological peptides, and in particular to a mussel peptide and its application in the preparation of uric acid-lowering products. The amino acid sequence is shown in SEQ ID NO.1‑4. The present invention prepares mussel polypeptides by composite enzyme-directed enzymatic hydrolysis and membrane separation and purification technology, and screens out four novel polypeptides with potential biological activity by combining LC‑MS / MS analysis and activity prediction. Molecular docking and cell experiments have confirmed that these four polypeptides can specifically inhibit GLUT9 protein activity and reduce uric acid levels; animal experiments have further verified their efficacy in lowering uric acid, providing a scientific basis for the high-value development of mussel resources and the development of functional uric acid-lowering products.

The invention discloses application of ZmCOP1a and ZmCOP1b proteins and coding genes of the ZmCOP1a and ZmCOP1b proteins in regulation and control of plant yield. The invention belongs to the technical field of biology, and particularly relates to application of ZmCOP1a and ZmCOP1b proteins and coding genes thereof in regulation and control of plant yield. The proteins ZmCOP1a and ZmCOP1b or expression substances for regulating genes or substances for regulating the activity or content of the proteins can be applied to any one of the following applications: U1) application in regulating the plant yield; the invention also relates to application of the U3) in preparation of products for regulating and controlling plant yield, application of the U3) in preparation of products for regulating and controlling plant yield, application of the U4) in preparation of products for cultivating plants with changed plant yield, and application of the U5) in plant breeding.

The present application relates to a microorganism having weakened erythronate-4-phosphate dehydrogenase protein activity; a method for producing O-phosphoserine, cysteine ​​and cysteine ​​derivatives using the same; a composition for producing O-phosphoserine containing the microorganism; and use of the microorganism for producing O-phosphoserine, cysteine ​​and cysteine ​​derivatives.

The present invention relates to compositions and methods for subpopulation-specific modulation of cell function and for treating repeat expansion disorders comprising genetic, degenerative, neurological and cellular diseases, including immune disorders. Also provided are research kits for subpopulation-specific modulation of protein activity and the corresponding potential to discover novel therapeutic compositions. More specifically, the disclosed compositions and methods selectively up- or downregulate at least a first population of a cellular protein or therapeutic target with minimal or negligible effect on the activity of at least a second population of the cellular protein or therapeutic target.