5 results about "Protein chip" patented technology

The utility model relates to the field of automatic product quality inspection, in particular to an automatic protein chip quality inspection device based on machine vision. Comprising the steps that an X-axis moving platform drives a camera to horizontally move in the X-axis direction, a Y-axis moving platform drives a bearing tray to horizontally move in the Y-axis direction, the camera is fixed to the X-axis moving platform, the bearing tray is used for bearing a protein chip, and the camera is in communication connection with a PC terminal to transmit image data; a positioning calibration module is further arranged to compensate mechanical errors, a light source module provides a detection light source, and the surface of the bearing tray is provided with an anti-skid coating and / or a positioning groove. And the PC end configures an image splicing control module to splice the local images of the blocks into a global view in combination with the position encoder. The technical effects that automatic quality inspection of the protein chip is achieved, the quality inspection efficiency and accuracy are improved, the stability of the chip during movement is ensured, and a complete chip image is obtained are achieved.

The utility model discloses a fixing device of a protein detection chip, which comprises a table body, the top end of the table body is fixedly connected with a table top, the top end of the table top is fixedly provided with a placing table, two sides of the top end of the table top are fixedly provided with two clamping parts, and the rear part of the top end of the table top is provided with a moving mechanism. According to the fixing device for the protein detection chip, the clamping part is arranged, so that the protein detection chip can be clamped and fixed, the size and the thickness of the chip can be adjusted, and the length of the telescopic end of the air cylinder is adjusted to meet the requirement for clamping chips of different sizes; the vertical sliding of the upper clamping block is adjusted to meet the requirement for clamping chips with different thicknesses, higher adaptive capacity is achieved to adapt to protein detection chips with different specifications, the optical detector can be moved to any point above the placement table by arranging the moving mechanism, the optical detector has higher moving freedom degree, and the detection efficiency is improved. Meanwhile, the detection work of protein chips with different specifications can be met.

The application belongs to the field of microfluidic protein chips, and specifically discloses a microfluidic protein chip for allergen detection, which comprises a sample adding area and a microfluidic unit, the microfluidic unit comprises a dilution area, a premixing area, a labeling area, a quantification area and a detection area which are connected in sequence, wherein the sample adding area comprises a first sample adding area and a second sample adding area, the first sample adding area is used for adding a whole blood sample, and the second sample adding area is used for adding a serum or plasma sample, different samples can be distinguished and processed; the first sample adding area is designed ingeniously by setting a capillary channel as a valve; a vibrator is arranged in the premixing area to drive a mixing ball to vibrate, so that the mixing efficiency is improved, the mixing of the sample and the diluent is more sufficient, the mixing time is reduced, and the difference of detection results is reduced; the detection result difference is further reduced through quantification of each sub-quantification area; and the labeling area and the detection area can be coated with multiple antibodies, and detection of multiple to-be-detected substances can be simultaneously performed.

This invention provides a protein chip for early warning of liver cancer and its application. The protein chip includes an antigen set; the antigen set includes antigens; the antibodies corresponding to the antigens are at least one of 61 selected from GNA11, GNAS, IDH1, MSH2, NPM1, PAX5, PTCH1, PTEN, SRSF2, Survivin, CAST, PAIP1, PRKCZ, RAD23A, RUNX1T1, SARS, TP53, APEX2, RCSD1, and AIF1; the protein chip is used for early warning assessment of liver cancer, and the early warning assessment steps include: collecting serum from the target population, using the protein chip to detect the serum to obtain detection data, and then determining whether they belong to a low-risk, medium-risk, or high-risk population based on a risk warning model; the protein chip systematically detects tumor-related autoantibody risk factor signals using high-throughput technology for early warning detection of liver cancer.

The application provides a background noise suppression system for a microarray protein chip, and relates to the technical field of chip detection, and comprises a collection module, which is used for collecting an original fluorescence image of the microarray protein chip by a laser confocal scanner at a preset excitation power, and taking the original fluorescence image as a to-be-processed signal matrix; an extraction module, which is used for locating three reference entities pre-solidified on a chip substrate from the to-be-processed signal matrix, the three reference entities being located at non-array regions of the upper left corner, the center point and the lower right corner of the chip respectively, and each reference entity being composed of inert fluorescent microspheres with a known concentration; and the mean values of the fluorescence intensities of local windows where the three reference entities are located are extracted respectively as discrete sampling values of spatial heterogeneity background noise. The application improves the low-abundance protein signal detection rate, reduces the quantitative deviation of the array edge, and improves the detection repeatability and quantitative accuracy.