433 results about "Protein chip" patented technology

The invention discloses an integral detecting reacting board and protein chip agent box of Carcinoembryonic antigen,CEA, 15-3 CA15-3(Carbohydrate Antigen 15-3,CA15-3), CA 125 (Cancer antigen 125,CA125), Squamous cell carcinoma antigen,SCC, Human papillomavirus antibody,HPVAb, beta-human chorionic gonadotropin, beta-HCG and alpha-fetoprotein,AFP, wherein the reacting hole board contains base and reacting hole on the base; the reacting hole contains 2-384 sample holes, 2-200 standard sample holes; the fixing phase carrier is set on the bottom of each reacting hole, which is covered by seven antigen or antibody molecule arrays with anti-CEA, anti-CA15-3,anti-CA125,anti-SCC,HPV,anti- beta-HCG,anti-AFP.

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Disclosed herein is a thin-film layered centrifuge device and an analysis method using the same. One example of an embodiment of the present invention is a thin film layered centrifuge device where a device, such as a lab on a chip, a protein chip and a DNA chip, for diagnosing and detecting a small amount of material in a fluid is integrated into a rotatable thin-film layered body, and to an analysis method using the thin-film layered centrifuge device.

A defect detecting apparatus captures an image of a protein chip formed on each die of a wafer with a low magnification for every first division region obtained by dividing each die in plurality; stores each obtained image as an inspection target image together with an ID for identifying each first division region; creates a model image for every first division region by calculating an average luminance value of pixels of each inspection target image; extracts a difference between the model image and each inspection target image as a difference image; determines presence of a defect by extracting a Blob having an area larger than a preset value from the difference image; captures a high-magnification image of every second division region; creates a model image again and extracts a Blob; and determines the kind of the defect based on a feature point of the defect.

Embodiments of the invention provide methods, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and/or late HPV-associated or HPV-specific proteins or antibodies. Detection of HPV DNAs, genomes, and/or oncoproteins by protein chips immunological assays can be used in early clinical screening for HPV infection and general diagnosis for cervical cancer and can be advantageous performed in a multiplexed test. Comparative detection of altered levels of HPV proteins and host proteins can performed in one or more assays. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer.

The invention provides a systemic detection method of a tumor marker and application thereof. The method comprises the following steps: 1) providing a sample from a checking object, wherein the checking object is a person; 2) detecting the sample of step 1), wherein in the detection step, whether various protein tumor markers exist or not is determined by utilizing high-throughput nucleic acid sequencing and determination comprising polygene mutation, polygene methylation, a plurality of micro RNAs (Ribonucleic Acid) and the like and utilizing a protein chip. According to the systemic detection method of the tumor marker and the application thereof, the different tumor markers are subjected to systemic detection including DNA (Deoxyribonucleic Acid), RNA, protein and the like, so that thesensitivity of early-stage detection of the tumors is improved.

The invention relates to a method and system of adopting space phase modulation and interference array to detect the biochip, which belongs to biological technical field. The invention aims to overcome the deficiency of current technique and provides a new biochip detecting method and system, which can detect different volumes of DAN chips and protein chips dispense with mark and real-time achieve the information about the interaction of the biomolecules. The principle of the detection by space phase modulation and interference array is: the light reflected by the biosensor unit entering wallaston prism after combined by the one dimensional magnifying lens, the contained s ray and p ray which is polarizationdirection is divided into the two rays transmitting in different direction and realizing co-lightpath space phase modulation and interference in wallaston prism; the interference fringe being converted into electrical signal through the polarizer and imaging lens, the information of real-time phase change of the each unit on the array chip being acquired after processing and be offered to the biologist and medical scientists to parse.

The invention discloses a male multi-tumor marker detection protein chip and a kit thereof. The chip comprises a substrate, protein markers distributed in an array type and point coatings of contrasts, wherein the substrate is a glass substrate or a film substrate; and the tumor markers and the point coatings of the contrasts are seven protein markers of AFP, CEA, NSE, CYFRA21-1, CA19-9, tPSA and SCC-ag, a positive contrast and a negative contrast which are uniformly distributed and latticed on the substrate. A reaction result of various indexes can be obtained only through one reaction by utilizing the protein chip, and the following tumors can be simultaneously screened: primary liver cancer, prostatic cancer, pancreatic cancer, lung cancer, esophageal cancer, gastric cancer and colorectal cancer. The invention is particularly suitable for the general examination of malignant tumors of male asymptomatic groups and high risk groups.

The present invention relates to a method of diagnosing colorectal cancer in human samples using several novel protein markers. The markers have been identified by assaying a number of tissue and serum samples from healthy individuals and persons diagnosed with colorectal cancer by means of protein chip technology using mass spectrometry. Differential expression pattern of these markers are indicative of a person having colorectal cancer patient. The diagnosis is based on comparing at least one intensity value, obtained using the method, to a reference value.

A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg/ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.8) to prepare an antigen solution for immobilization; placing a petri dish comprising the slide on a spotting guide and spotting 1-100 μl of the antigen solution on spotting points; and performing a reaction on the slide prepared as described above for 1-6 hours to immobilize the antigen, and an immune-fluorescence slide prepared by using the method.

The invention discloses a protein chip kit for detecting inflammatory factors, which comprises a basement membrane (1) and a reactant and a detection agent (2). A plurality of kinds of specific antibodies are fixed on the basement membrane; the specific antibodies and the inflammatory factors can undergo an antigen-antibody reaction and each specific antibody is respectively fixed on the basementmembrane for forming a plurality of independent recognition loci; and the reactant and the detection agent are used for detecting whether substances capable of undergoing the antigen-antibody reaction with the specific antibodies exist in a sample to be detected or not by an array-ELISA method. The invention also discloses a method for preparing the protein chip kit for detecting the inflammatoryfactors, which comprises a step of fixing the specific antibodies on the basement membrane. The kit of the invention adopts protein chip technology, can detect 40 inflammatory factors, overcomes a plurality of shortcomings and has the advantages of low cost, convenient use, high sensitivity and accuracy, high flux, small using amount of samples, capability of being popularized in an ordinary laboratory, massive production and the like.

The invention relates to a cell surface receptor biochip based on a surface plasmon resonance biosensor, a preparation method and application thereof. The biochip is characterized in that the chip is provided with a gold surface coating at a glass substrate and the gold surface is fixed with a sephadex layer which is fixed with the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin and surface receptors are fixed by antibody capture. The preparation method includes that the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin adopts the method of antibody capture and is fixed on the surface of CM5 chip based on the surface plasmon resonance biosensor, so as to produce the protein chip of para-insulin receptors which is applicable to the mutual action between IGF-1R and IRS-1, SHC, PI3K or GRB2 and hopeful to be applied to screening cancer-fighting drugs.

The invention discloses a self-immunity hepatitis detection protein chip and a kit thereof. The chip and the kit can be used for simultaneously detecting 12 immunity protein markers: ANA (dsDNA, RNP, Sm, SS-A/Ro, SS-B/La, Sc1-70, Jo-1), SMA, LKM-1, SLA/LP, F-actin and AMA. The invention overcomes the shortcomings of the current products that the detection index is single and incomplete. The invention provides a detection method which has a complete diction result and a simpler process.

The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

A thin film bio valve device and an apparatus for controlling the thin film bio valve device are effectively applied in a valve structure of a thin film device, such as a lab-on-a-chip, a protein chip, or a DNA chip, for diagnosing and/or detecting a small amount of material in a fluid.

The invention relates to a sensing method of a protein array chip and its detecting system in the field of biology technique. The method comprises the following steps: letting the light reflected by the protein array chip to get through a one-dimensional magnifying lens array and inject into a time are phase modulator, changing the voltage to let the ejected s light and p light to get through the polarizing prism to generate intervention, changing the image into the electric signal by CCD after imaging lens and inputting it into the computer; when changing a phase of p light, it collects an image; using the collected five images as a circulation to obtain the time phase changing information aroused by the reaction of each unit of the array chip.

The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.

The invention relates to an early diagnosis kit and an early warning kit, in particular to an early diagnosis and early warning kit for transplanted kidney rejection reaction and a use method of the kit, and further to some proteins applied in the early warning of transplanted kidney acute rejection reaction or transplanted kidney rejection reaction. By applying a liquid protein chip (luminex) detection technique, the expression level of 96 cytokines/chemokines and receptors thereof in 266 serum samples from 142 patients having acute transplanted kidney rejection reaction and transplant recipients with stable function of transplanted kidneys are monitored and comprehensively analyzed. An early warning system for the transplanted kidney rejection reaction comprising at least one combined marker and an early diagnosis system for the transplanted kidney rejection reaction comprising at least one factor combined marker are established. The prediction and accuracy rate of the acute rejection achieve 98.6%, and the internal cross validation rates are 97.1% and 97.2%, respectively.

A detection kit of multiindex protein for diagnosing cardiovascular disease is prepared as having base plate and reaction holes on it set on reaction hole plate; including 12-200 sample holes and 5-8 standard piece holes in said reaction holes as each reaction hole bottom being set with solid phase carrier; enveloping micro lattice of eight antibody as anti-CRP, anti-Myoglobin, anti-cTnI, anti-cTnT, anti-NT-proBNP, anti-CK-MB, anti-H-FABP and anti-GPBB on said carrier for accurately diagnose said disease in multiple man-share.

The linear light beam scanned surface plasma resonant imaging light intensity detection method and system belongs to the field of biological detection technology. In the incident optical path, one vibrating mirror driven with one vibration and oscillation driver is set to alter the incident angle to the sensing surface and to complete linear light beam scanning. When the biomolecules in the surface of the protein chip act mutually, the vibration mirror reflected light radiates to the sensing surface in constantly changed angle to excite the surface plasma on the protein chip to resonate constantly, so as to obtain corresponding light intensity signal with the photoelectronic detector. Once the vibration mirror frequency is fixed, the detector signal is recorded to complete the detection. The present invention has simple system structure, high sensitivity, high precision and capacity of multichannel detection.