16 results about "Protein chip" patented technology

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

The invention belongs to the field of medicine detection, and particularly discloses a comprehensive control method for the quality of compound Pien Tze Huang lozenges, which comprises the following steps of: fishing targeted protein active ingredients of the compound Pien Tze Huang lozenges by respectively adopting a surface plasma resonance instrument with a built-in TNFR2 protein chip, a built-in TLR4 protein chip or a built-in TLR5 protein chip; the biological effect of the compound Pien Tze Huang lozenge is detected by adopting an SPR method based on the fished anti-inflammatory active ingredients, and the fingerprint spectrum of the compound Pien Tze Huang lozenge and the content of the ingredients in the fingerprint spectrum are detected by adopting an UPLC method. The surface plasmon resonance method and the UPLC method are combined to control the quality of the compound Pien Tze Huang lozenge, evaluation of the overall drug effect and accurate determination of component composition are combined, the quality of the compound Pien Tze Huang lozenge is controlled more comprehensively, and the method has practical application value.

The invention provides application of C1q complement protein in preparation of an element for detecting Abeta42 protein, a chip for detecting the Abeta42 protein and application of the chip. The C1q complement protein is used as an element for specifically recognizing the Abeta42 protein, the C1q complement protein can be fixed on the chip body, and the obtained chip can be used for preparing a specific element, such as a biosensor. Compared with the prior art, when the biosensor based on the dual-polarization interference technology is used for detecting the Abeta42 protein, synthesis of a complex nano material or design of a complex signal transduction pathway is not needed, and the biosensor has the advantages of being easy and convenient to operate, high in selectivity and high in renewability.

The present invention relates to a kind of Angong Niuhuang Wan quality evaluation method based on STAT3 protein biological effect, it is to use surface plasmon resonance (SPR) detection technology, specifically including the following steps: 1) construction of protein chip;2) preparation of test solution;3) preparation of reference solution;4) test solution and reference solution injection are taken respectively and carry out SPR detection, analyze and flow through protein chip surface and STAT 3 protein binding, measure corresponding response value (RU value).Quality evaluation standard is that the ratio of test sample RU value to reference substance RU value should be not less than 1.The present invention is optimized and verified by methodology, and accuracy is high, can reflect the effectiveness of Angong Niuhuang Wan clinical treatment, further improves the quality control system of Angong Niuhuang Wan, possesses practical application promotion value.

The utility model relates to the field of automatic product quality inspection, in particular to an automatic protein chip quality inspection device based on machine vision. Comprising the steps that an X-axis moving platform drives a camera to horizontally move in the X-axis direction, a Y-axis moving platform drives a bearing tray to horizontally move in the Y-axis direction, the camera is fixed to the X-axis moving platform, the bearing tray is used for bearing a protein chip, and the camera is in communication connection with a PC terminal to transmit image data; a positioning calibration module is further arranged to compensate mechanical errors, a light source module provides a detection light source, and the surface of the bearing tray is provided with an anti-skid coating and / or a positioning groove. And the PC end configures an image splicing control module to splice the local images of the blocks into a global view in combination with the position encoder. The technical effects that automatic quality inspection of the protein chip is achieved, the quality inspection efficiency and accuracy are improved, the stability of the chip during movement is ensured, and a complete chip image is obtained are achieved.

The utility model discloses a fixing device of a protein detection chip, which comprises a table body, the top end of the table body is fixedly connected with a table top, the top end of the table top is fixedly provided with a placing table, two sides of the top end of the table top are fixedly provided with two clamping parts, and the rear part of the top end of the table top is provided with a moving mechanism. According to the fixing device for the protein detection chip, the clamping part is arranged, so that the protein detection chip can be clamped and fixed, the size and the thickness of the chip can be adjusted, and the length of the telescopic end of the air cylinder is adjusted to meet the requirement for clamping chips of different sizes; the vertical sliding of the upper clamping block is adjusted to meet the requirement for clamping chips with different thicknesses, higher adaptive capacity is achieved to adapt to protein detection chips with different specifications, the optical detector can be moved to any point above the placement table by arranging the moving mechanism, the optical detector has higher moving freedom degree, and the detection efficiency is improved. Meanwhile, the detection work of protein chips with different specifications can be met.

The utility model discloses a microfluidic protein chip, which belongs to the technical field of biochips and diagnostic reagents and comprises an upper shell and a lower shell, the upper shell and the lower shell are connected in a clamping manner, a sample introduction window is arranged on the upper shell, and a through hole is arranged on the upper shell; a sample adding area, a reaction area and a micro-fluidic area are arranged in the lower shell, the sample adding area is located at one end of the reaction area, and the micro-fluidic area is arranged at the other end of the reaction area; the micro-fluidic area comprises a micro-fluidic channel and a waste liquid tank, and after the upper shell and the lower shell are covered, the through hole is formed in the upper part of the waste liquid tank. The micro-fluidic protein chip disclosed by the utility model is simple to manufacture and convenient to operate, not only can accurately control the reaction time, but also can realize the recovery of waste liquid and avoid environmental pollution.

The invention belongs to the technical field of autoimmune encephalitis, and particularly relates to an autoimmune encephalitis biomarker combined detection protein chip and a kit, the protein chip comprises a substrate and a marker antibody coated in the substrate, the substrate is provided with at least one sample application module, the sample application module is provided with the marker antibody, and the marker antibody is coated in the substrate. The kit comprises a protein chip, a sample diluent, a cleaning buffer solution and fluorescent group labeled goat anti-human IgG, and the autoimmune encephalitis biomarker combined detection protein chip and kit have the advantages of high sensitivity, strong specificity, high accuracy, stable result, good repeatability, convenient and fast operation, manpower and material resource saving, and high detection efficiency. The kit is a reliable tool means for early diagnosis of autoimmune encephalitis by medical workers, and the labor efficiency is improved. The patient can be treated earlier, and the survival rate is increased.

The utility model discloses a microporous percolation protein detection chip, which belongs to the technical field of biochips and diagnostic reagents, and comprises an upper shell and a lower shell, the upper shell is connected with the lower shell in a clamping manner, a microporous membrane is arranged between the upper shell and the lower shell, the upper surface of the upper shell is provided with a concave cavity, a sample introduction window is arranged in the cavity, and the sample introduction window is connected with the microporous membrane. A sample introduction window is formed in the upper surface of the lower shell, a sample introduction hole is formed in the sample introduction window, the lower end of the sample introduction hole is connected with a sample introduction channel, the bottom of the sample introduction channel is connected with a reaction area on the microporous membrane, the lower end of the reaction area is connected with a sample outlet channel, and the lower end of the sample outlet channel is connected with a sample outlet hole in the upper surface of the lower shell. The protein chip disclosed by the utility model can reduce the use amount of samples, is accurate in measurement result, and does not cause environmental pollution.

The application belongs to the field of microfluidic protein chips, and specifically discloses a microfluidic protein chip for allergen detection, which comprises a sample adding area and a microfluidic unit, the microfluidic unit comprises a dilution area, a premixing area, a labeling area, a quantification area and a detection area which are connected in sequence, wherein the sample adding area comprises a first sample adding area and a second sample adding area, the first sample adding area is used for adding a whole blood sample, and the second sample adding area is used for adding a serum or plasma sample, different samples can be distinguished and processed; the first sample adding area is designed ingeniously by setting a capillary channel as a valve; a vibrator is arranged in the premixing area to drive a mixing ball to vibrate, so that the mixing efficiency is improved, the mixing of the sample and the diluent is more sufficient, the mixing time is reduced, and the difference of detection results is reduced; the detection result difference is further reduced through quantification of each sub-quantification area; and the labeling area and the detection area can be coated with multiple antibodies, and detection of multiple to-be-detected substances can be simultaneously performed.

The utility model discloses a protein chip for detecting respiratory viruses, which belongs to the technical field of biochips and diagnostic reagents and comprises an antigen loading membrane, a reverse osmosis membrane layer, a water absorption membrane layer and a leakage-proof membrane layer are sequentially fixed on the lower surface of the antigen loading membrane, and a detection area is arranged on the upper surface of the antigen loading membrane. The detection area comprises a plurality of antigen loading areas and a quality control area, a respiratory tract specific antigen is fixedly loaded on each antigen loading area, and the respiratory tract specific antigens comprise chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, syncytial virus, influenza B virus and parainfluenza virus. The percolation type protein chip can be used for detecting six pathogens related to the respiratory tract, and is simple in method and high in practicability.

This invention provides a protein chip for early warning of liver cancer and its application. The protein chip includes an antigen set; the antigen set includes antigens; the antibodies corresponding to the antigens are at least one of 61 selected from GNA11, GNAS, IDH1, MSH2, NPM1, PAX5, PTCH1, PTEN, SRSF2, Survivin, CAST, PAIP1, PRKCZ, RAD23A, RUNX1T1, SARS, TP53, APEX2, RCSD1, and AIF1; the protein chip is used for early warning assessment of liver cancer, and the early warning assessment steps include: collecting serum from the target population, using the protein chip to detect the serum to obtain detection data, and then determining whether they belong to a low-risk, medium-risk, or high-risk population based on a risk warning model; the protein chip systematically detects tumor-related autoantibody risk factor signals using high-throughput technology for early warning detection of liver cancer.

The application provides a background noise suppression system for a microarray protein chip, and relates to the technical field of chip detection, and comprises a collection module, which is used for collecting an original fluorescence image of the microarray protein chip by a laser confocal scanner at a preset excitation power, and taking the original fluorescence image as a to-be-processed signal matrix; an extraction module, which is used for locating three reference entities pre-solidified on a chip substrate from the to-be-processed signal matrix, the three reference entities being located at non-array regions of the upper left corner, the center point and the lower right corner of the chip respectively, and each reference entity being composed of inert fluorescent microspheres with a known concentration; and the mean values of the fluorescence intensities of local windows where the three reference entities are located are extracted respectively as discrete sampling values of spatial heterogeneity background noise. The application improves the low-abundance protein signal detection rate, reduces the quantitative deviation of the array edge, and improves the detection repeatability and quantitative accuracy.

The present invention relates to the technical field of protein chips, and discloses a protein chip with a high-density porous microstructure and a preparation method thereof, comprising a polymer substrate, a porous microstructure layer fixed on the polymer substrate by photocrosslinking, and protein antigen molecules fixed on the activated porous microstructure layer; the polymer substrate refers to a polymer containing C-H bonds. Photocrosslinking is a preparation method with low energy consumption and low cost. It adopts a polymer containing C-H bonds as a substrate material, and crosslinks the porous microstructure layer and the polymer substrate together by photocrosslinking. The process is simple and fast, and the porous structure of the porous microstructure layer greatly increases the contact area with the protein antigen molecules, which is conducive to the high-density aggregation of protein antigen molecules, and effectively reduces the lowest limit of antibody concentration detected by the protein chip. In addition, the preparation process of the protein chip is simple and fast, with low energy consumption and low cost.

The utility model relates to the technical field of medical drug detection equipment, and discloses a sample processing device for protein chip detection, which comprises two support frames and a plurality of uniformly distributed clamping plates, the upper ends of the adjacent sides of the two support frames are provided with first chutes, and the lower ends of the adjacent sides of the two support frames are provided with fixing components. According to the utility model, the two sides of the clamping plates are fixedly provided with the fixing plates, the two fixing plates are connected through the low-temperature-resistant tension spring, and the clamping plates are provided with the plurality of low-temperature-resistant silica gel convex blocks, so that the two clamping plates can be connected together through the low-temperature-resistant tension spring; according to the sampling tube fixing device, the low-temperature-resistant silica gel protruding blocks are arranged on the supporting plate, the placed sampling tube is clamped and fixed through the low-temperature-resistant silica gel protruding blocks, meanwhile, the cylindrical protruding blocks are fixed in the grooves formed in the supporting plate, the sampling tube can be supported, and therefore the number of freezable contact points between the sampling tube and the fixing device can be effectively reduced while the sampling tube is fixed.

This application discloses a method for protein chip detection of brucellosis, relating to the technical field of brucellosis detection. The method involves spotting Brucella synergistic composite antigens for multi-index combined detection onto a pretreated solid-phase carrier, drying, and then washing. A blocking agent is then added for blocking, followed by washing to obtain a protein chip. An initial sample is then collected, diluted, centrifuged, and the supernatant is collected. The supernatant is added to the protein chip site, incubated with shaking, washed, and then a secondary antibody is added for further incubation and washing. Finally, a fluorescence scanner is used to read the signal, subtract the background, and determine whether the sample is positive or negative. This method has a wide detection range, strong specificity, and high sensitivity, and has a very broad application prospect.