Microfluidic protein chip

Abstract

The utility model discloses a microfluidic protein chip, which belongs to the technical field of biochips and diagnostic reagents and comprises an upper shell and a lower shell, the upper shell and the lower shell are connected in a clamping manner, a sample introduction window is arranged on the upper shell, and a through hole is arranged on the upper shell; a sample adding area, a reaction area and a micro-fluidic area are arranged in the lower shell, the sample adding area is located at one end of the reaction area, and the micro-fluidic area is arranged at the other end of the reaction area; the micro-fluidic area comprises a micro-fluidic channel and a waste liquid tank, and after the upper shell and the lower shell are covered, the through hole is formed in the upper part of the waste liquid tank. The micro-fluidic protein chip disclosed by the utility model is simple to manufacture and convenient to operate, not only can accurately control the reaction time, but also can realize the recovery of waste liquid and avoid environmental pollution.

Description

Technical Field

[0001] The utility model relates to the technical field of biochips and diagnostic reagents, in particular to a microfluidic protein chip. Background Art

[0002] Protein chips are cells that have been fixed in an orderly manner on a carrier (such as a filter membrane, gel, glass slide, nanobeads, and microplate) to analyze and detect components in the sample that can specifically interact with them.

[0003] Microfluidics refers to the science and technology involved in systems that use microchannels (with dimensions of tens to hundreds of micrometers) to process or manipulate tiny fluids (with volumes of microliters, nanoliters, or even attoliters). It is an emerging interdisciplinary subject involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering.

[0004] The research on combining microfluidic technology with protein chips for biological detection is of great significance. Chinese patent CN 115634723 A discloses a microfluidic protein chip for allergen detection, which achieves the technical effect of small difference in detection results of the same sample between different batches or the same sample in the same batch, and good repeatability by setting multiple groups of microfluidic channels around the sample and rotating the sample to flow into different channels. Chinese patent CN 113145189 A discloses a protein chip and a detection module containing the same, which can accurately control the reaction time between the protein chip and the sample by setting multiple protein-carrying membranes on the flow path of the microfluidic channel and covering the entire cross-section of the flow path, and can simultaneously perform multiple reactions on the same sample without interfering with each other.

[0005] The shortcomings of the above patent are that its production cost is high, its principle is complex, it has high requirements for users and detection equipment, and it does not collect and treat waste liquid, which can easily cause biological contamination. Utility Model Content

[0006] The technical problem to be solved by the utility model is to design a novel microfluidic protein chip, which is simple to manufacture and easy to operate, and can not only accurately control the reaction time, but also realize the recycling of waste liquid and prevent environmental pollution.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions: a microfluidic protein chip, comprising an upper shell and a lower shell, the upper shell and the lower shell are snap-connected, an injection window is provided on the upper shell, and a through hole is provided on the upper shell;

[0008] A sample adding area, a reaction area and a microfluidic area are arranged inside the lower housing, wherein the sample adding area is located at one end of the reaction area, and the microfluidic area is arranged at the other end of the reaction area;

[0009] The microfluidic area includes a microfluidic channel and a waste liquid tank. After the upper shell and the lower shell are covered, the through hole is located on the upper part of the waste liquid tank.

[0010] Furthermore, the reaction area includes a protein chip placement area and a waste liquid collection area, and the waste liquid collection area is connected to the microfluidic channel.

[0011] Furthermore, the shape of the waste liquid collection area changes from wide to narrow, and the narrower end thereof is connected to the microfluidic channel.

[0012] Furthermore, the microfluidic channel is bent.

[0013] Furthermore, a liquid baffle is provided inside the lower shell, and the liquid baffle surrounds the sample adding area, the reaction area and the microfluidic area.

[0014] Furthermore, the liquid baffle plate includes a first-zone liquid baffle plate and a second-zone liquid baffle plate, the first-zone liquid baffle plate surrounds the sample addition zone, and the second-zone liquid baffle plate surrounds the microfluidic zone.

[0015] Furthermore, a protein chip is placed in the reaction area. Beneficial effects

[0016] The present application sets a microfluidic zone at the end of the reaction zone, and sets a waste liquid tank at the end of the microfluidic zone, and sets a through hole on the upper shell body. The position of the through hole is set above the waste liquid tank. When in use, the pipe connected to the liquid pump is extended into the waste liquid tank through the through hole to collect the waste liquid after the reaction. Not only can the waste liquid be collected, but the reaction time can also be controlled, thereby eliminating environmental pollution.

[0017] By setting up liquid baffles around the sample addition area, reaction area and microfluidic area, and designing the shape of the liquid baffles to block the three areas separately, the sample is prevented from mixing with the waste liquid after the reaction, which will affect the reaction results.

[0018] The application is simple in design and easy to operate, and is suitable for large-scale clinical promotion and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the microfluidic protein chip of the utility model.

[0020] Figure 2 This is a schematic diagram of the structure of the lower shell of the utility model Figure 1 .

[0021] Figure 3 This is a structural diagram of the liquid baffle of the utility model.

[0022] Figure 4 This is a schematic diagram of the structure of the lower shell of the utility model Figure 2 .

[0023] Among them, 1-upper shell, 2-lower shell, 3-injection window, 4-through hole, 5-sample addition area, 6-reaction area, 61-protein chip placement area, 62-waste liquid collection area, 7-microfluidic area, 71-microfluidic channel, 72-waste liquid tank, 8-liquid baffle, 81-zone one liquid baffle, 82-zone two liquid baffle. DETAILED DESCRIPTION

[0024] In order to enhance the understanding of the present invention, the present invention will be described in detail below with reference to embodiments and drawings. The embodiments are only used to explain the present invention and do not limit the scope of protection of the present invention.

[0025] like Figure 1 The figure shows a microfluidic protein chip, which comprises an upper shell 1 and a lower shell 2, which can be snap-fitted together. An injection window 3 and a through hole 4 are provided on the upper shell 1.

[0026] like Figure 2 As shown, the interior of the lower housing 2 is respectively provided with a sample loading area 5, a reaction area 6 and a microfluidic area 7, wherein the sample loading area 5 is located at the lower end of the sample loading window 3 and is connected to one end of the reaction area 6. The reaction area 6 includes a protein chip placement area 61 and a waste liquid collection area 62. The protein chip 9 is placed in the protein chip placement area 61. During the reaction, the sample gradually flows from the protein chip placement area 61 into the waste liquid collection area 62, and flows out from the end of the waste liquid collection area 62 to the microfluidic area 7. In order to facilitate the waste liquid collected in the waste liquid collection area 62 to flow into the microfluidic area 7, the waste liquid collection area 62 is designed to be narrowed from wide to narrow, such as a funnel shape, and the narrow part is connected to the microfluidic area 7.

[0027] The microfluidic area 7 includes a microfluidic channel 71 and a waste liquid tank 72. The microfluidic channel 71 is bent, such as a U-shaped bend or a wavy bend. The purpose is to increase the length of the microfluidic channel 71 and facilitate the regulation of the sample flow rate.

[0028] When the upper shell 1 and the lower shell 2 are covered, the through hole 4 is located just above the waste liquid tank 72. Its purpose is to facilitate the pipe connected to the liquid pump to reach the waste liquid tank 72 through the through hole 4 and extract the waste liquid in the waste liquid tank 72.

[0029] According to a preferred embodiment of the present invention, a liquid baffle 8 is provided inside the lower housing 2, and the liquid baffle 8 surrounds the sample addition area 5, the reaction area 6 and the microfluidic area 7. The specific shape of the liquid baffle 8 is as follows: Figure 3 and Figure 4As shown, the liquid baffle plate 8 includes a first-zone liquid baffle plate 81 and a second-zone liquid baffle plate 82, wherein the first-zone liquid baffle plate 81 is arranged around the sample adding area 5 to surround the sample adding area 5, and the second-zone liquid baffle plate (82) is arranged around the microfluidic area 7 to surround the microfluidic area 7. The shape of the liquid baffle plate 8 determines that the liquid baffle plate 8 can distinguish the sample adding area 5, the reaction area 6 and the microfluidic area 7 respectively.

[0030] During use, a pipe connected to the liquid pump is extended through the through hole 4 into the waste liquid tank 72. After the sample is added to the sample addition area 5 through the sampling window 3, it is adsorbed by the protein chip 9 placed in the reaction area 6. After the liquid pump is started, the suction force provided by the liquid pump to the microfluidic area 7 causes the sample to continuously flow into the microfluidic area 7. By controlling the magnitude of the suction force, the liquid flow rate can be controlled, thereby controlling the reaction time. After the sample reacts and flows into the waste liquid tank 72, the liquid pump is used to extract the sample and collect it in a device specifically for storing waste liquid until the reaction is complete.

[0031] The embodiments disclosed in the present invention are preferred embodiments, but are not limited thereto. Ordinary technicians in this field can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. As long as they do not deviate from the spirit of the present invention, they are all within the scope of protection of the present invention.

Claims

1. A microfluidic protein chip, comprising an upper shell (1) and a lower shell (2), wherein the upper shell (1) and the lower shell (2) are snap-connected, and an injection window (3) is provided on the upper shell (1), characterized in that: A through hole (4) is provided on the upper shell (1); A sample addition area (5), a reaction area (6) and a microfluidic area (7) are provided inside the lower housing (2), wherein the sample addition area (5) is located at one end of the reaction area (6), and the microfluidic area (7) is provided at the other end of the reaction area (6); The microfluidic area (7) includes a microfluidic channel (71) and a waste liquid tank (72). After the upper shell (1) and the lower shell (2) are covered, the through hole (4) is located at the upper part of the waste liquid tank (72).

2. The microfluidic protein chip according to claim 1, characterized in that The reaction area (6) includes a protein chip placement area (61) and a waste liquid collection area (62), and the waste liquid collection area (62) is connected to the microfluidic channel (71).

3. The microfluidic protein chip according to claim 2, characterized in that The shape of the waste liquid collection area (62) changes from wide to narrow, and its narrower end is connected to the microfluidic channel (71).

4. The microfluidic protein chip according to claim 1, characterized in that The microfluidic channel (71) is bent.

5. The microfluidic protein chip according to claim 1, characterized in that A liquid baffle (8) is provided inside the lower shell body, and the liquid baffle (8) surrounds the sample addition area (5), the reaction area (6) and the microfluidic area (7).

6. The microfluidic protein chip according to claim 5, characterized in that The liquid baffle plate (8) includes a first-zone liquid baffle plate (81) and a second-zone liquid baffle plate (82), wherein the first-zone liquid baffle plate (81) surrounds the sample addition zone (5), and the second-zone liquid baffle plate (82) surrounds the microfluidic zone (7).

7. The microfluidic protein chip according to claim 1, characterized in that A protein chip (9) is placed in the reaction area (6).

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