The invention provides a biological determination method for determining resistance of VIP3A
gene-modified corn
grassland spodoptera frugiperda based on a feed blending method, and relates to the technical field of determination of
insect resistance of transgenic
insect-resistant corn, and the biological determination method comprises the following three steps: preparing a sample, preparing a toxic feed, setting a control group, and performing biological determination and
data acquisition. According to the method, highly uniform distribution of test substances in the feed is realized in a
powder mixing manner, so that accurate
dose effect analysis can be carried out, meanwhile, the steps of
protein extraction, purification and substantial equivalence analysis are omitted, the process is simplified, and the cost is reduced. The method has the advantages that the method is particularly suitable for early-stage and rapid high-
throughput screening of a large number of transgenic breeding materials, the real situation that pests eat transgenic plants in the field is simulated to the greatest extent under controllable experimental conditions, and the
evaluation result has higher
predictive value. The actual resistance performance of the pests in the field is more truly reflected.