56 results about "Helicoverpa armigera" patented technology

The invention discloses a Bacillus thuringiensis MB-15 strain which has a broad spectrum and high virulence on vegetable lepidoptera pests, and the preservation number of the strain is CGMCC No. 5255. The strain can form a diamond insecticidal crystal protein, contains cry1I, cry1Ac, cry2Ac and vip3Aa genes, and has high-efficiency insecticidal activity on plutella xylostella, Pieris brassicae, beet armyworm, Spodoptera litura, Helicoverpa armigera and other lepidoptera pests. The invention also relates to a preparation method of the wettable powder of strain. The wettable powder comprises the following content of main components: 40.0-50.0% of Bacillus thuringiensis MB-15 strain powder, 5.0-10.0% of a dispersant, 5.0-10.0% of a wetting agent, 0.5-1.0% of a stabilizer, 0.5-1.0% of an ultraviolet light protection agent, and 28.0-49.0% of a carrier. The wettable powder can be used to control a plurality of lepidoptera pests on vegetables, and has the characteristics of broad spectrum, high efficiency, and safety, etc.

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

The invention discloses helicoverpa armigera V-ATPase A gene cDNA and application thereof. Helicoverpa armigera V-ATPase A siRNA is designed and synthesized, after helicoverpa armigera takes V-ATPase A siRNA in, expression of V-ATPase A gene is blocked, growth and metabolism of helicoverpa armigera are inhibited, the mortality is raised, and therefore helicoverpa armigera V-ATPase A gene cDNA is applicable to biological control of helicoverpa armigera.

The invention relates to the technical field of agriculture microorganism genetic engineering, in particular to separation and cloning of a Helicoverpa armigera cadherin gene fragment hacad1and encoded polypeptide fragment HaCad1. By separation, the cadherin gene fragment hacad1 of 735bp is obtained from the midintestine of the Helicoverpa armigera, an encoded product thereof is the polypeptide fragment HaCad1 which can be combined with Bt toxin CrylAc10. Biological experiments indicate the polypeptide prepared in the invention has very strong enhancing insecticidal activity on insecticidal crystal protein CrylAc10 of lepidoptera insects. The invention also includes preparation and an application for recombinant Escherichia coli EMB1104 expressing the Helicoverpa armigera cadherin fragment HaCad1. The recombinant Escherichia coli EMB1104 is preserved in CCTCC, the preservation number is CCTCC NO: M209010.

The invention discloses a composite insecticidal and acaricidal nano aqueous agent containing fenpropathrin and lambda-cyhalothrin, wherein the composite insecticidal and acaricidal nano aqueous agentis prepared from active ingredients of fenpropathrin and lambda-cyhalothrin, a surfactant and water, the weight percentage content of the fenpropathrin and the lambda-cyhalothrin is 3-80%, the weightpercentage content of a pesticide adjuvant is 5-20%, the balance is water, and a weight ratio of fenpropathrin to lambda-cyhalothrin is (20:1)-(1:20). The insecticidal composition can generate high synergistic effect, overcomes and delays the drug resistance of pests, is high in insecticidal speed and long in lasting period, reduces the medication cost, has obviously high prevention and control effect compared with a single agent, can prevent and control lepidoptera pests on crops, especially can be used for preventing and controlling helicoverpa armigera and tetranychus cinnabarinus, and hassignificantly high effect compared with the use of a single agent.

The invention belongs to the technical field of bioengineering and provides a biological medicament that has recombinant gene expression of helicoverpa armigera C-type lectin and two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene. The helicoverpa armigera C-type lectin gene and the two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene have recombinant expression in Escherichia coli. The invention further provides a recombinant gene expression method of the helicoverpa armigera C-type lectin and the two carbohydrate recognizing structural domains of Ha-CRD1 and Ha-CRD2 of the gene. The lectin can reduce the insect fatal rate of pathogenic organisms, improve the pathogenic organism phagocytizing efficiency of blood corpuscle, and reduce the survival number of pathogenic organisms in insect bodies. The biological medicament does not generate drug-fast bacteria, does not have adverse effect to the normal vital activity of insects, can reduce the usage of antibiotic, and can reduce the drug-fast bacteria generation in environments.

The invention provides a method and process for preparing tung oil pesticide. The method comprises the following steps: refining tung oil with alkali, mixing with an emulsifier and uniformly mixing with other auxiliaries. The tung oil has effects of killing pests and inhibiting bacteria. The tung oil pesticide is simple in preparation, has a good pest killing effect and wide market application prospect, has the characteristics of fast action and long action, and can be used for reducing the usage of chemical pesticide and preventing pests from generating drug resistance. The tung oil pesticide does not have pollution in production and processing processes, has stable product quality, wide insecticidal spectrum, strong activity and convenience in use, and can be used for preventing and controlling beauveria bassiana, argyroneta aquatica, cabbage caterpillar, helicoverpa armigera, aphid, green leafhopper, cockroaches, acarid and the like.

The invention relates to the field of insecticides, particularly to a medicinal plant insecticide and a preparation method thereof. The medicinal plant insecticide is prepared from, by weight part, 15-35 parts of tobacco extract, 10-30 parts of ginkgo leaf extract, 8-25 parts of sophora root extract, 8-25 parts of derris trifoliata extract, 30-35 parts of ternate pinellia extract, 0.5-1 part of emulsifier, 0.5-1 part of penetrant and 0.5-1 part of stabilizer. By taking tobacco, ginkgo leaves, sophora root, derris trifoliata and ternate pinellia as the principal raw materials and processing different raw materials, the prepared medicinal plant insecticide can achieve significant killing effects of higher than 80% on plutella xylostella, helicoverpa armigera, aphidoidea and spodoptera exigua.

The invention discloses a protein GmSqm associated with the insect tolerance of plants as well as a coding gene and application thereof. The protein is the following a) or b): a) a protein with an amino acid sequence shown in a sequence 1 in a sequence table; b) a protein which is obtained by carrying out substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence shown in the sequence 1 in the sequence table and is associated with the insect tolerance of plants. Experiments prove that compared with wild arabidopsis thaliana plants and trans-empty vector arabidopsis thaliana plants, trans-GmSqm arabidopsis thaliana plants have the advantages that after newly-hatched helicoverpa armigera Hubner larvae are fed with the trans-GmSqm arabidopsis thaliana plants, the weights of the helicoverpa armigera Hubner larvae are obviously reduced, and the body length of the helicoverpa armigera Hubner larvae is obviously reduced. The protein GmSqm is of great importance in cultivating plants tolerant to insects.

The invention discloses a method for obtaining a large amount of campoletis chorideae uchida wild populations. The method uses lepidoptera larvae to be manually released before tobacco leaves or cornleaves grow vigorously to trap campoletis chorideae uchida, and comprises the following specific steps of slightly brushing the lepidoptera larvae on tender leaves of the tobacco leaves or the corn leaves by using a writing brush before the tobacco leaves or the corn leaves grow vigorously; and trapping the campoletis chorideae uchida to lay eggs on the lepidoptera larvae, and manually collectingwild population cocoons of the campoletis chorideae uchida within a fixed time limit. The method further comprises the step that attractants are smeared on the tender leaves of the tobacco leaves or the corn leaves at the same time, and inducers are one or a mixture of more of host plant volatile matter, nectariferous plant extracts and parasitic information gland compounds. Compared with the prior art, the method for obtaining a large number of campoletis chorideae uchida wild populations provided by the invention can obtain a large number of populations within the fixed time limit, and is used for indoor propagation and rejuvenation.

The invention discloses a plant-derived self-emulsifying nano-insecticide as well as a preparation method and application thereof. The plant-derived self-emulsifying nano-insecticide is prepared fromthe following components: 2-5% of crude ethanol extract from ginkgo biloba exocarp, 2.5-3% of surface active agent and 2-2.5% of cosurfactant; the mass ratio of a solvent-ethanol to a ginkgo biloba exocarp crude extract is equal to 1 to 1. The application objects of the nano-insecticide are pests including lepidoptera plutella xylostella, armyworm, helicoverpa armigera, and the like. The plant-derived self-emulsifying nano-insecticide has the advantages that anhydrous self-emulsifying nanoemulsion is introduced into pesticide by adopting the natural surface active agent, so that the biologicalactivity of the natural product-derived pesticide can be effectively improved, and the production quality and yield are better improved; furthermore, the plant-derived self-emulsifying nano-insecticide reduces the production cost, has a control effect of higher than 90% for all the pests, and also has a control effect of higher than 90% for aphids.

The invention discloses applications of 3,4-dimethylbenzaldehyde, particularly applications of the 3,4-dimethylbenzaldehyde in preparation of pest attractants, wherein the 3,4-dimethylbenzaldehyde isone of volatile secondary metabolites synthesized by Chinese rose plants. According to the invention, the active component is separated from the Chinese rose headspace volatile matter, and the results of GC-EAD, EAG, olfactory behavior, oviposition behavior biological tests and field oviposition tests prove that the 3,4-dimethylbenzaldehyde has good attracting effect on pests, especially Helicoverpa armigera female moths; and the 3,4-dimethylbenzaldehyde is a natural secondary metabolite and has a complete degradation path in nature, so that the 3,4-dimethylbenzaldehyde is used as a main component to prepare the pest attractant so as to achieve the pollution-free prevention and control of pests.

The present invention relates to a nitrogen-containing bridged ring compound having insect disinfestation activity, preparation and uses thereof, and particularly provides a compound having a structure represented by a general formula I, a preparation method and uses thereof, wherein R1, R2, R3, R4, R5, R6, R7, R8, R, n and Z are defined in the specification. The present invention further relates to an agricultural composition containing the compound or a pesticide acceptable salt, and uses of the agricultural composition. According to the present invention, the compound provides high insect disinfestation activity on Homoptera pests, Lepidoptera pests and other agricultural and forestry pests such as aphid, planthopper, whiteflies, leafhoppers, thrips, Helicoverpa armigera Hubner, Pieris rapae, diamondback moth, Spodoptera litura, armyworms and the like. The general formula I is defined in the specification.

The invention relates to a synergistic pesticide composition containing lambda-cyhalothrin and hexaflumuron. The effective ingredient of the synergistic pesticide composition is a binary combination of the lambda-cyhalothrin and the hexaflumuron, wherein the mass ratio of the lambda-cyhalothrin to the hexaflumuron is (1:50) to (50:1), and the mass ratio of the effective ingredients, namely the lambda-cyhalothrin and the hexaflumuron in a preparation is 1-80%, and the balance is an auxiliary ingredient. The dosage form of the pesticide composition is water dispersible granules, wettable powder, a suspending agent, missible oil,a microemulsion or an emulsion in water. The synergistic pesticide composition is mainly used for preventing and controlling lepidoptera pests, such as helicoverpa armigera, heliothis virescens, tobacco hornworm, diamond back moth, armyworm, beet armyworm, spodoptera frugiperda, cabbage argyrogramma agnate, imported cabbageworm, flowering cabbage, cabbage strip moth, manduca quinquemaculata, rice-stem borer, rice leaf folder and ostrinia nubilalis. The synergistic pesticide composition has a significant synergistic effect, the insecticidal activity is obviously strengthened in comparison with that of a single dosage, the dose of a pesticide is reduced and the environmental pollution is reduced.

The invention discloses a pesticide composition for preventing and controlling cotton field helicoverpa armigera and application thereof. According to the pesticide composition for preventing and controlling the cotton field helicoverpa armigera and the application thereof, after the compounding of glyphosate and an organosilicone additive, quick action, control effect and persistence period of prevention and removal of helicoverpa armigera host plant Abutilon theophrasti Medic. by the glyphosate are accelerated, wherein after the compounding use of 300, 400 and 500 times of the glyphosate with 1000 times of the silicone additive, the control effect reaches 85% or more in 5 days and reaches 100% in 28 days after the use of the pesticide composition, and is much higher than the single-agentcontrol effect of the glyphosate; after the compounding of emamectin benzoate and the organosilicone additive, quick action, control effect and persistence period of prevention and control of the helicoverpa armigera are improved, and pest insecticide resistance is delayed, wherein the control effect is increased by 20% in 48 hours after the use of the pesticide composition; and a ternary compounding composition of the glyphosate, the emamectin benzoate and the organosilicone additive plays a dual effect of prevention and control of the helicoverpa armigera and simultaneously prevention and removal of the cotton field helicoverpa armigera host plant Abutilon theophrasti Medic., and plays an effect of two-preventing by one-spraying with a reduced pesticide using amount, thereby reducing labor cost.

The invention relates to streptomyces with double functions of preventing diseases and killing pests, an application and a culture method thereof, and a biocontrol agent thereof, and belongs to the technical field of plant pest control. In the invention, an endophytic streptomyces MS001 with bacteriostatic activity is separated and obtained. The fermentation liquor has stronger bacteriostatic activity on Botryosphaeria dothidea, Colletotrichum gloeosporioides, Phomopsis mali, Rhizoctonia cerealis, Gaeumannomyces graminis, Botrytis cinerea, Fusarium graminearum, Fusarium oxysporum and Colletotrichum karstii, and the bacteriostatic objects have broad-spectrum property; and the fermentation liquor has the function of inhibiting the growth of plant pests such as helicoverpa armigera and monochamus alternatus larvae. Therefore, the streptomyces MS001 can prevent and control some diseases and pests on apple trees, garden plants, vegetables and food crops.