Method for clean purifying L-tryptophan by utilizing fermented liquid

Abstract

The invention relates to a method for clean purifying L-tryptophan by utilizing fermented liquid, which is applied in the purification of L-tryptophan. The method sequentially comprises the following steps of (1.1) microfiltration, (1.2) ultrafiltration, (1.3) nanofiltration, (1.4) cryoconcentration, (1.5) conventional centrifugal separation to obtain crude products and take the produced filtrate as mother liquid which is returned to (1.1) microfiltration for recycling, (2.1) preparation of 35% alcohol solution of L-tryptophan crude products to be purified, (2.2) decolorization of active carbon, (2.3) filtration, (2.4) cooling, mixing, crystallization and centrifugation and (2.5) conventional procedures of washing, drying and packaging; the filtration in (2.3) is conventional filtration by plate frames; and the washing in (2.5) adopts alcohol aqueous solution for washing and returns filtrate and washing liquid produced therefrom as the mother liquid to (2.2) decolorization of active carbon for recycling. The method has the advantages of recycling of the mother liquid, short purification time, high purification purity, cost saving and the like.

Description

technical field [0001] The invention relates to a method for cleaning and purifying L-tryptophan by using fermented liquid, which is applied to purify L-tryptophan. Background technique [0002] The scientific name of L-tryptophan is β-indolylalanine, the chemical name is L-2-amino-3-indolylpropionic acid, alias L-trypsin amino acid, L-aminoindolepropionic acid, molecular formula C 11 h 12 o 2 N 2 , relative molecular mass 204.21. L-Tryptophan (L-Tryptophan) is a neutral aromatic amino acid containing indolyl, white or slightly yellow leaf-like crystals or powder, odorless or slightly smelly, soluble in hot pyridine, slightly soluble in ethanol , Insoluble in chloroform and ether. It is soluble in dilute acid or alkali, and it will be colored under long-term light. Heating with water produces a small amount of indole, such as in NaOH or CuSO 4 In the presence of heating, a large amount of indole is produced. Unstable when heated with acid in the dark. It is relative...

AI Technical Summary

Problems solved by technology

Its disadvantage is that a large amount of organic reagents such as glacial acetic acid and inorganic reagents such as sulfuric acid and ammonia are added during the extraction process of the crude product of L-tryptophan.

Its disadvantage is that the L-tryptophan to be purified is dissolved in water to produce a low-concentration aqueous solution, and it must be concentrated to 6-10% of the original volume, resulting in a long time for the subsequent process to be concentrated in vacuum, which consumes a lot of energy and inevitably Causes damage to L-tryptophan products with high heat sensitivity

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