Production method for extracting tryptophan from fermentation liquor by one-step refining
A production method and technology of tryptophan, which is applied in the field of biochemical engineering, can solve the problems of low extraction yield, environmental pollution, and easy deterioration of products, and achieve the effects of high extraction yield, reduced cost, and less membrane pollution
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[0035] Example 1:
[0036] The first step is to pre-treat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO of the fermentation broth volume respectively 3 Then, stir and heat to 60°C for 30 minutes to prepare the treatment liquid;
[0037] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the pressure difference between the inlet and outlet membranes is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 The purified water is top-washed until the tryptophan content in the concentrate is less than 1.0g / L, and then the machine is shut down to obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria are removed. The sterilization rate reaches 99.74%, and L-tryptophan The yield was 99.3%;
[0038] The third s...
Example Embodiment
[0046] Example 2:
[0047] The first step is to pre-treat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO of the fermentation broth volume respectively 3 Then, stir and heat to 60°C for 30 minutes to prepare the treatment liquid;
[0048] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the pressure difference between the inlet and outlet membranes is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 The purified water is top-washed until the tryptophan content in the concentrate is less than 1.0g / L, and then the machine is shut down to obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria are removed. The sterilization rate reaches 99.74%, and L-tryptophan The yield was 99.3%;
[0049] The third s...
Example Embodiment
[0057] Example 3:
[0058] The first step is to pre-treat the fermentation broth containing L-tryptophan: the treatment method is to add 0.1% alum and 0.1% NaHSO of the fermentation broth volume respectively 3 Then, stir and heat to 60°C for 30 minutes to prepare the treatment liquid;
[0059] The second step, the treatment liquid is pumped into the ceramic microfiltration membrane filter, the temperature is controlled at ±45℃, the pressure difference between the inlet and outlet membranes is 0.2~0.3MPa, and the filtration speed is controlled at 2~3m 3 / h, when the clear liquid flow is less than 2m 3 / h, divided into multiple times, each pumping 1.5~3m 3 The purified water is top-washed until the tryptophan content in the concentrate is less than 1.0g / L, and then the machine is shut down to obtain the tryptophan microfiltrate and the bacterial protein from which the bacteria are removed. The sterilization rate reaches 99.74%, and L-tryptophan The yield was 99.3%;
[0060] The third s...
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