The invention discloses a traditional Chinese medicine disinfection spray and a preparation method thereof, relates to the technical field of traditional Chinese medicines and mainly aims to solve the problem of low disinfection capability of the disinfection spray in the prior art. The effective components for preparing the spray comprise the following raw materials in parts by mass: 11-23 parts of coptis chinensis, 9-21 parts of cordate houttuynia, 14-28 parts of persicaria perfoliata, 10-25 parts of honeysuckle, 6-18 parts of senecio scandens, 17-29 parts of radix sophorae flavescentis, 8-26 parts of dandelion, 17-31 parts of plantain herb, 7-22 parts of calamus, 12-27 parts of garlic, 10-24 parts of peach leaves, 13-25 parts of radix asparagi, 6-23 parts of pine needle, 11-29 parts of fructus cnidii, 7-26 parts of radix stemonae, 12-28 parts of fructus crataegi, 7-25 parts of mint, 9-25 parts of radix angelicae, 8-25 parts of angelica sinensis and 12-27 parts of liquorice. The prepared traditional Chinese medicine disinfection spray has excellent bacteria removal effect; the sterilizing rate against the bacteria in the air reaches over 99%.