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66 results about "Virus processing" patented technology

The main idea behind viral processing is to stop the viruses in a given sample from contaminating the desired product. The two most widely used methods of viral processing are viral removal and viral inactivation. The former is a method in which all viruses are simply removed from the sample completely. The latter method is one in which the viruses may remain in the final product, but in a non-infective form. These techniques are used widely in the food and blood plasma industries, as those products can be harmed by the presence of viral particles. Some of the more common viruses removed by these methods are the HIV-1 and HIV-2 viruses; hepatitis A, B, and C; and parvoviruses. The methods used in the plasma industry have been summarized (Horowitz B., Minor P., Morgenthaler J. J., Burnouf T., McIntosh R., Padilla A., Thorpe R. and van Aken W. G. Who Expert Committee on Biological Standardization. World Health Organ Tech Rep Ser. 924: 1-232, 2004.) In some cases, however, it is the virus itself that is the desired product, as is often the case with the HIV. In many cases, researchers may be trying to extract the viruses from the blood for study, not specifically for blood purification. It is also common to use these types of techniques to remove particles produced as a result of viral infection.

Preparation of virus release buffer solution and application thereof

The invention provides a virus release buffer solution and a method for improving the blastochyle virus titer by use of the virus release buffer solution. The method comprises the following steps of: fetching the virus release buffer solution and the virus blastochyle respectively; centrifuging the virus blastochyle at an intermediate speed to separate the precipitate I and supernate I; adding the virus release buffer solution to the precipitate; mixing uniformly, and stirring for 10-30 minutes at a stirring speed of 1,000-5,000r / min at a temperature of 2-8 DEG C; centrifuging again at an intermediate speed to separate the precipitate II and supernate II; mixing the supernate II and supernate I and performing transverse tangential-flow concentration; or performing transverse tangential-flow concentration of the supernate II and supernate I respectively before mixing to obtain the concentrate of the virus blastochyle, wherein the aperture of a filter membrane for the transverse tangential-flow concentration is 10-500KDa, and the blastochyle concentration multiple is 2-10. According to the invention, the detection virus titer of the blastochyle avian virus can be improved; after virus treatment, the stability of the virus particles can be enhanced without causing influence on the virus antigen; and moreover, a great quantity of blastochyle viruses can be treated, and the treatment speed is high.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA +1
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