Preparation of virus release buffer solution and application thereof

Abstract

The invention provides a virus release buffer solution and a method for improving the blastochyle virus titer by use of the virus release buffer solution. The method comprises the following steps of: fetching the virus release buffer solution and the virus blastochyle respectively; centrifuging the virus blastochyle at an intermediate speed to separate the precipitate I and supernate I; adding the virus release buffer solution to the precipitate; mixing uniformly, and stirring for 10-30 minutes at a stirring speed of 1,000-5,000r / min at a temperature of 2-8 DEG C; centrifuging again at an intermediate speed to separate the precipitate II and supernate II; mixing the supernate II and supernate I and performing transverse tangential-flow concentration; or performing transverse tangential-flow concentration of the supernate II and supernate I respectively before mixing to obtain the concentrate of the virus blastochyle, wherein the aperture of a filter membrane for the transverse tangential-flow concentration is 10-500KDa, and the blastochyle concentration multiple is 2-10. According to the invention, the detection virus titer of the blastochyle avian virus can be improved; after virus treatment, the stability of the virus particles can be enhanced without causing influence on the virus antigen; and moreover, a great quantity of blastochyle viruses can be treated, and the treatment speed is high.

Description

technical field [0001] The invention belongs to the field of veterinary vaccines, and in particular relates to a virus release buffer and a method for using the virus release buffer to increase the virus titer of embryo fluid and prepare conjoined vaccines. Background technique [0002] Poultry vaccines are mainly produced by propagating viruses in chicken eggs. The vaccine has the characteristics of simple preparation process, reliable immune effect and long duration of immunity. It has been used in poultry in many countries and regions, and has played a certain positive role in preventing and controlling the outbreak of bird flu. However, inactivated vaccines have low immunization efficiency, large immunization doses, and it is difficult to distinguish vaccine immunization and wild infection of epidemic diseases during monitoring, and there are also disadvantages such as the risk of loose virus. In addition, the use of traditional inactivated vaccines to prevent avian inf...

AI Technical Summary

Problems solved by technology

Although many people have used different methods to separate and purify avian influenza virus, these methods obviously have the following disadvantages: small processing capacity, such as high-speed centrifugation; expensive, such as ion exchange chromatography; influence on the purity of virus antigens , such as using chemical reagents such as ether to extract viruses from concentrated embryo fluid

These factors are not conducive to industrialization

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