Process for preparing veterinary rabies inactivated and freeze-dried vaccine through suspension culture cell

Abstract

The invention relates to a process for preparing a veterinary rabies inactivated vaccine, in particular to a process for preparing a veterinary rabies inactivated and freeze-dried vaccine through a suspension culture cell. The method has the key point that: a bioreactor is used for large-scale suspension culture of baby hamster kidney BHK21-C13 cells and rabies virus is inoculated, and the rabiesvirus is subjected to mass propagation through a fed-batch and perfusion technology, so that a high-concentration and high-titer rabies antigen is obtained and concentrated, inactivated and purified to prepare the veterinary rabies inactivated vaccine; and thus, technical problems such as complexity, low antigen content, low effectiveness, large dosage, poor batch-to-batch variation and the like existing in the conventional process are solved.

Description

technical field

[0001] The invention relates to a method for manufacturing an inactivated rabies vaccine for veterinary use, in particular to a process for producing an inactivated rabies lyophilized vaccine for veterinary use by suspension culture cells. Background technique

[0002] About 93% of Chinese rabies patients are caused by domestic dogs, 6% are caused by cats, and a few are caused by pigs and mice. Therefore, the localization of veterinary rabies inactivated vaccines and domestic animals are regularly injected with rabies inactivated vaccines It is an important way to control and eliminate rabies in our country. At present, the production of veterinary inactivated rabies vaccines in China uses the traditional rotary bottle process, which is cumbersome and has low antigen content, which cannot meet the quality requirements. At present, there is no supply in the market. All veterinary inactivated rabies vaccines are completely dependent on imports, which are expens...

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