3results about How to "Small reaction volume" patented technology

The invention relates to an enzymatic determination method of beta-hydroxybutyric acid, a kit thereof and a use method of the kit, the determination method comprises the following steps: generating NADH by using D-3-hydroxybutyric acid dehydrogenase in the presence of NAD < + >, reducing Fe < 3 + > into Fe < 2 + > by using the NADH in the presence of 1-methoxy-5-methylphenazinium methyl sulfate (1-Methyl PES), then reacting Fe < 2 + > with Nitoso-PSAP to generate a colored substance, and determining the beta-hydroxybutyric acid by using the colored substance. The colored substance has a maximum absorption peak at 756nm, and the content of beta-hydroxybutyric acid is determined by detecting the absorbance of the colored substance. The kit comprises a reagent R1 and a reagent R2, the reagent R1 is prepared from D-3-hydroxybutyrate dehydrogenase, FeCl3 and a buffer solution; and the reagent R2 is prepared from NAD (Nicotinamide Adenine Dinucleotide) < + >, 1-Methoxy PES (Polyether Sulfonate), Nitroso-PSAP (Phosphatidylcholine) and a buffer solution. The linear correlation coefficient of the kit is greater than 0.999; the linear range is not less than 4mmol / L, and the polypeptide can resist the interference of vitamin C, bilirubin, fat emulsion, hemoglobin and uric acid, and is still stable in 7 days in an environment at 37 DEG C.

The invention provides a method and device for preparing 5, 5 '-oxydimethylene-2-furfural, and belongs to the technical field of chemical synthesis. The method for preparing the 5, 5 '-oxydimethylene-2-furfural comprises the following steps: mixing 5-hydroxymethylfurfural, a catalyst and an organic solvent in a micro-mixer to obtain a mixture; the mixture is continuously conveyed into a dynamic tubular reactor to be subjected to a self-etherification reaction, a reaction solution containing 5, 5 '-oxydimethylene-2-furfural is obtained, and the inner diameter of the dynamic tubular reactor is 60-80 mm.

This invention relates to a continuous flow synthesis method for hydroquinone, a chemical and pharmaceutical intermediate, belonging to the field of chemical intermediate synthesis. The method for preparing hydroquinone uses phenol as a starting material, which undergoes oxidation to obtain p-benzoquinone, followed by hydrogenation reduction to obtain hydroquinone. Both steps are performed in a microreactor system. The synthesis method includes the following steps: (1) Phenol, catalyst 1, and organic solvent are prepared as component A, and an oxidant is used as component B. A and B react in a microchannel reactor 1 to obtain intermediate I benzoquinone; (2) Intermediate I benzoquinone is mixed with an organic solvent and reacted with hydrogen in a microchannel reactor 2 under the action of catalyst 2 to obtain hydroquinone. This invention utilizes a microreactor system with excellent heat and mass transfer performance, significantly shortening the reaction time. Both steps are completed within 50 minutes, and the reaction efficiency is high, energy consumption is low, safety is high, automation is high, and the environment is environmentally friendly.