58results about How to "Good commercial prospects" patented technology

The invention discloses a PCR (Polymerase Chain Reaction) chip based on droplet array. The chip is obtained by taking a mono crystalline silicon sheet or a glass sheet as a base material and adopting standard photoetching as well as wet etching technique. The mono crystalline silicon sheet or the glass sheet is the base material of the chip. A SiO2 oxide layer and a silane layer generated by silanization on the surface of the SiO2 oxide layer are adhered to the base material successively. A hydrophilic pit array, which can be a droplet array and is formed by etching with photoresist technology, is disposed on the silane layer. A closed circular guardrail is arranged on the outer periphery of the hydrophilic pit array and the guardrail is connected with the silane layer in a sealed manner. The invention also discloses the application of the chip on real-time quantitative PCR detection and real-time quantitative isothermal amplification reaction detection. The detection system provided in the invention remarkably reduces the reaction volume and the detection sensitivity of the system fairly equals that of a commercialized quantitative PCR instrument. The detection system in the invention is easy for operation and has low cost, thus can be applied to commercialization.

The invention provides a method for amplifying colloidal gold immunochromatographic colorimetric signal intensity. The method comprises the following steps: closing a gold-labelled antibody by using amantadine marked bovine serum albumin to obtain a gold-labelled antibody I; synthesizing colloidal gold nanoparticles by using beta-cyclodextrin to obtain a beta-cyclodextrin coated colloidal gold solution; after adding a to-be-tested sample into a system which executes double-antibody sandwich colloidal gold immunochromatography by using the gold-labelled antibody I, adding the beta-cyclodextrincoated colloidal gold solution; and adding a mixed solution of 5,10,15,20-tetra(4-carboxyl phenyl)porphyrin and the beta-cyclodextrin coated colloidal gold solution into the system repeatedly for manytimes. According to the method, net-shaped nano-gold is formed by utilizing supermolecular self-assembling principle mediation and circular signal amplification is realized, so that the developing intensity of the god nanoparticles on a detection line and a quality control line is greatly improved and the aim of performing on-site rapid detection on a target detection object with ultralow concentration can be fulfilled. The invention also provides a high-sensitivity kit applied to colloidal gold immunochromatography.

The invention relates to a method for improving the resistance accuracy of a buried resistor in a PCB (printed circuit board), which comprises the following steps of: a step 1 of carrying out precompensation on an expected resistance of the buried resistor when the PCB is made into a finished product according to the resistance change rate of the buried resistor in the subsequent processing link so as to obtain a target resistance for making the buried resistor, and determining the length and the width of the buried resistor according to the target resistance; a step 2 of carrying out copper foil removal processing on a line layer on which the buried resistor needs to be made and making a square groove which has corresponding size and is used for accommodating the buried resistor at a preset position of the line layer; a step 3 of carrying out silk-screen printing in the square groove by adopting a screen printing plate to form a layer of resistor ink and baking and curing the resistance ink to form a buried resistor to be modified; and a step 4 of carrying out on-line dynamic modification and regulation on the buried resistor to be modified by adopting a resistor testing instrument and a laser ablation machine until the buried resistor with the target resistance is obtained. The method for improving the resistance accuracy of the buried resistor in the PCB, which is provided by the invention, can be used for obtaining the buried resistor with the high accuracy resistance.

A laser device is composed of actively mode-locking ring optical fibre laser device and cavity length stabilized feedback loop. Said ring laser device is composed of dispersion compensating fibre (DCF), adjustable optical band-pass filter, polarizer, polarization holding fibre, M-Z model LiNbO3 electro-optical modulator, two optical couplers, optical isolator, optical fibre amplifier and electrically controlled optical delay line. It can increase the wavelength stability of laser device to + / - 0.01 nm.

The invention relates to a sensitization type immunochromatographic kit for sensitively detecting a novel coronavirus antibody, which introduces a controllable copper growth technology to amplify theoptical signal intensity of a colloidal gold probe of a detection line on the basis of a colloidal gold immunochromatographic test strip so as to realize high-sensitivity detection of an object to bedetected. The kit comprises the colloidal gold immunochromatography test strip and a copper growth solution, wherein the colloidal gold immunochromatography test strip is formed by sequentially fixinga sample pad, a combination pad, a nitrocellulose membrane and absorbent paper on a PVC plastic bottom plate; the copper growth solution comprises a copper growth solution A and a copper growth solution B. The kit provided by the invention is simple to operate, high in sensitivity, good in stability, high in detection speed (20-30 minutes) and easy in result reading, does not need other auxiliaryinstruments and equipment, and can be used for on-site rapid ultra-sensitive detection and large-scale epidemiological investigation of clinical and emergency events.

The invention relates to lithium-sulfur battery electrolyte capable of improving battery capacity and a preparation method thereof. The electrolyte is prepared from organic solvents, a lithium salt and an additive and is characterized in that the organic solvents include a first solvent, a second solvent and a third solvent; the first solvent is one or more of 1,3-dioxolane, 1,4-dioxane and 1,1-dimethoxyethane; the second solvent is one or more of glycol dimethyl ether and diglyme; the third solvent is one or more of dimethyl sulfide organics; the volume ratio of the first solvent to the second solvent is (0.5 to 2) : 1, and the volume fraction of the third solvent in the organic solvents is 10 to 40%. Dimethyl ether organics are reduced into methyl lithium sulfur and can contribute capacity except positive active materials. By means of the unique work principle, lithium sulfur batteries prepared from the electrolyte have the characteristics of achieving and even exceeding the theoretical capacity of the lithium sulfur batteries.

The invention provides a preparation method of a nitrogen and sulfur co-doped graphene oxide grafted polyaniline / MoS2 electrode material. According to the method, graphene oxide is used as a carrier, pyrrole is used as a nitrogen source, thiophene is used as a sulfur source, and nitrogen and sulfur co-doped graphene oxide is obtained through a series of polymerization, activation, pre-oxidation and carbonization; the nitrogen and sulfur co-doped graphene oxide is subjected to acid activation, thionyl chloride modification, p-phenylenediamine modification and aniline polymerization to obtain nitrogen and sulfur co-doped graphene oxide grafted polyaniline; and MoS2 is loaded onto the nitrogen and sulfur co-doped graphene oxide grafted polyaniline through a hot water reaction to obtain the nitrogen and sulfur co-doped graphene oxide grafted polyaniline / MoS2. According to the nitrogen and sulfur co-doped graphene oxide grafted polyaniline / MoS2 electrode, the specific capacitance is larger than 400 F / g under the condition that the current density is 1A / g, and the capacitance is 80% or above of the initial value after the nitrogen and sulfur co-doped graphene oxide grafted polyaniline / MoS2 electrode is recycled 800 times.

The invention relates to a nano nutritious supplementary with a function of fattening pigs and a preparation method thereof. The nano nutritious supplementary comprises the following components in parts by weight: 0.5-33 parts of vitamin A, 0.1-17 parts of vitamin D3, 0.6-24 parts of vitamin E, 0.1-12 parts of vitamin B1, 0.5-8 parts of vitamin B2 sodium phosphate, 0.01-1 part of vitamin B12, 0.2-13 parts of nicotinamide, 0.5-4 parts of calcium pantothenate, 0.1-8 parts of biotin, 0.4-2 parts of folic acid, 0.2-19 parts of vitamin C, 1-17 parts of glutamine, 0.2-4 parts of methionine, 0.6-7 parts of lysine, 0.1-3.9 parts of arginine, 0.8-6 parts of threonine, 2-25 parts of glycine, 10-70 parts of glucose, 1-10 parts of mannan oligosaccharide and 600-850 parts of distilled water. The nano nutritious supplementary has the functions of fattening pigs, and improving the stress resistance and the disease resistance of pigs.

The invention relates to application of a cry2Ah-vp gene in armyworm resisting. The applicant inserts a proline behind No. 354 bit valine of cry2Ah and a cry2Ah1-vp mutant shown as the SEQ ID No. 1 isobtained accordingly. The cry2Ah-vp gene is obtained after codon optimization, the cry2Ah-vp gene is transplanted into maize with an agrobacterium-mediated method, and T0 generation transgenic maizeis obtained. The experimental result shows the cry2Ah-vp gene can be normally transcribed into different tissues of the maize, the expression quantity of the cry2Ah-vp protein in all parts of the maize is the maximum in the six-leaf stage, the leaf expression quantity is 2.20 mu g / g (fresh weight), and all parts have higher expression in the tasseling stage and the filling stage. The insect resisting experiment result shows that the transgenic maize has good insecticidal activity to the armyworms, and the armyworms are all dead after inoculated to the maize three days; the cry2Ah-vp gene has aremarkable growth inhibition effect to the ostrinia nubilalis larvae.

The invention relates to a sandy kaolin based cordierite glass ceramic material. The sandy kaolin based cordierite glass ceramic material is prepared from raw materials in percentage by mass as follows: 25%-50% of sandy kaolin, 20%-26% of aluminum oxide, 7%-28% of silicon oxide, 10%-15% of magnesium oxide and 5%-7% of an aid. The invention further relates to a preparation method of the sandy kaolin based cordierite glass ceramic material. The glass ceramic material only contains a single physical phase which is alpha-cordierite phase, belongs to a magnesia-alumina-silica system and has a good physical property. The preparation method adopts a simple technological procedure, the sources of raw materials are abundant, aluminum-silicon elements in natural sandy kaolin are used as sources of elements for cordierite glass ceramic, sand removal and purification are not required, no tailings are produced, energy is saved, and the environment is protected; meanwhile, impurity ingredients in the sandy kaolin serve as an aid, can reduce the sintering temperature, reduce pores and promote generation of the alpha-cordierite phase and implementation of low-temperature co-sintering, and has great significance in industrial production.

The invention relates to the technical field of chemical synthesis, in particular to thio-octyl phosphoramido ester and a synthetic method and application thereof. The synthetic method of the thio-octyl phosphoramido ester includes the step of: adopting thioctic acid as a raw material, performing a reduction reaction to obtain thio-octanol, and performing an esterification reaction to obtain the thio-octyl phosphoramido ester. When the thio-octyl phosphoramido ester is used as a DNA end-group modifier, DNA with sulfur-containing modified end groups can be prepared efficiently and massively, and the simple and efficient DNA end-group modifier with controllable cost can be provided for sulfur-containing modification of a 5' end of DNA; meanwhile, the synthetic method has the advantages of aconcise path, convenient operation, a simple method for purifying intermediates and the product, and requirements of scientific research and business can be fully met.

The invention relates to a general-purpose livestock nano nutritional agent having an anti-stress function and a preparation method of the nutritional agent. The nutritional agent comprises following components by weight: 0.05-6 parts of vitamin A, 0.02-4 parts of vitamin E, 0.1-1.8 parts of vitamin B1, 0.2-7 parts of vitamin B2 and sodium phosphate, 0.1-3 parts of vitamin B6, 0.2-9 parts of nicotinamide, 0.2-3 parts of calcium pantothenate, 0.04-1.5 parts of folic acid, 0.2-19.4 parts of vitamin C, 0.5-2 parts of beta-carotene, 2-22 parts of glycine, 0.4-1.7 parts of methionine, 0.2-2.5 parts of lysine, 0.5-4.5 parts of glutamine, 0.8-6.4 parts of threonine, 12-45 parts of glucose, 1-15 parts of mannan oligosaccharide, 1.1-4.9 parts of astragalus polysaccharides and 700-900 parts of distilled water. The nutritional agent has functions of relieving livestock stress, and enhancing the anti-stress capability and disease resistance of the livestock.

The invention discloses a nano nutritional supplement for breeding promotion and stress resistance of breeding poultry and a preparation method thereof. The nano nutritional supplement comprises the following components in part by weight: 0.02 to 25 parts of vitamin A, 0.01 to 9 parts of vitamin D3, 0.4 to 13 parts of vitamin E, 5 to 23.7 parts of vitamin K3, 0.1 to 1.2 parts of vitamin B1, 0.7 to 8 parts of vitamin B2 sodium phosphate, 0.01 to 0.5 part of vitamin B12, 0.9 to 6 parts of nicotinamide, 0.5 to 4 parts of pantothenic acid, 0.4 to 6.8 parts of biotin, 0.04 to 2.6 parts of folic acid, 0.2 to 18.9 parts of vitamin C, 0.8 to 15 parts of glycine, 0.1 to 1.7 parts of methionine, 0.05 to 2.5 parts of lysine, 0.1 to 3.6 parts of arginine, 0.8 to 6.4 parts of threonine, 10 to 50 parts of glucose, 2 to 16 parts of mannan oligosaccharide, and 700 to 920 parts of distilled water. By the nano nutritional supplement, the improvement on the breeding performance of the breeding poultry can be promoted, and stress phenomena in a breeding poultry breeding process can be reduced.

The invention discloses kluyveromyces marxianus yeast and an application thereof in blueberry wine brewing. Kluyveromyces marxianus yeast with the preservation number of CCTCC No. M2017001 is obtained through separating and screening from a blueberry planting base. The kluyveromyces marxianus yeast is subjected to amplification culturing and then added into blueberry fruit pulp at an amount of 0.2-0.4g dried yeast (living bacteria count is 3-3.5*10<10>CFU / g) per kg of blueberry fruit pulp for brewing blueberry wine. Compared with the commercial wine yeast and other additives, the brewed blueberry wine has thicker fruit fragrance, more pure taste, and more comfortable mouthfeel, and has a simple process and stable quality, and is suitable for commercial production.

The invention provides a preparation method of a nitrogen and sulfur co-doped carbon fiber grafted polythiophene / MnS composite material and an electrode thereof. The wettability between the electrolyte and the electrode is improved by utilizing the high porosity and the large specific surface area of the carbon fiber skeleton; the polypyrrole is grafted onto the carbon fiber and is compounded with the manganese sulfide, so that the defect of low specific capacitance of a single carbon-based material is overcome, and the specific capacitance of the electrode material is greatly improved. As an electrode material of a supercapacitor, the nitrogen-sulfur co-doped carbon fiber grafted polythiophene / MnS composite material has the characteristics of stable preparation process, low cost, no pollution and the like, and has a good commercial prospect.

The invention relates to a preparation method and application of nitrogen-doped hollow carbon nanowire grafted polypyrrole. The invention provides a preparation method of a novel carbon-based electrode material. The preparation method comprises the following steps of 1, preparing an SiO2 nanowire; 2, preparing a nitrogen-doped carbon hollow nanowire; 3, modifying the nitrogen-doped carbon hollow nanowire with azobenzene; and 4, preparing a nitrogen-doped carbon hollow nanowire grafted polypyrrole electrode material. The electrochemical performance of the electrode material is tested, the specific capacitance is 301 F / g under the condition that the current density is 1 A / g, and the specific capacitance is 78.1% of the initial value after the electrode material is recycled for 800 times. The preparation method has the characteristics of stable process, easiness in operation, reliable quality, low cost, light weight, no pollution and the like, and has a good commercial prospect.

The invention relates to a preparation method of oily fluconazole injection for pets. The preparation method comprises the following steps: at first, carrying out dry heat sterilization on injection castor oil at 150 DEG C for 2 hours, cooling to 50 DEG C, dissolving fluconazole and butyl hydroxy anisd in a mixed solution of glyceryl triacetate and absolute ethyl alcohol, adding 1,2-propylene glycol for mixing, slowly adding the sterilized injection castor oil, stirring uniformly, filtering the prepared solution via a microfiltration membrane, and packaging the product in a sterilized penicillin bottle to obtain stable oily fluconazole injection for pets. The preparation process is simple and the cost is low, so that the commercialization prospect is good.

The invention relates to a preparation method of fumaric acid valnemulin. The preparation method comprises the following steps: firstly adding valnemulin in a methyl isobutyl ketone solution, then heating and stirring; after dissolving, adding absolute ethanol, adding fumaric acid until a reaction solution is clear; reducing the temperature with ice bath so as to separate out crystal; filtering, and washing filter cake with methyl isobutyl ketone; and drying so as to obtain the fumaric acid valnemulin, wherein the melting point of the fumaric acid valnemulin is larger than 130 DEG C, and the yield of the fumaric acid valnemulin reaches 85%.

The invention provides a preparation method of a lithium iron phosphate precursor. The preparation method of the lithium iron phosphate precursor comprises the following steps of: weighing lithium salt, ferrous salt and phosphate in a mol ratio of 3: (0.9-1.5): 1.2; preparing the lithium salt, the ferrous salt and the phosphate into a standard aqueous solution at a certain concentration; weighing a carbon source according to a mass fraction of 1-50 g / L; preparing the carbon source into a standard aqueous solution at a certain concentration; and quickly mixing the materials continuously or dropwise by using a high-shear mixer. By a high-shear mixing technology, precursor particles are mixed uniformly by an impinging stream, the particle size and the particle size distribution of the precursor particles are reduced, the electrochemical performance of a LiFePO4 / C material is improved as well as the reaction time is shortened; and the preparation method is small in equipment investment and simple in process operation, can achieve both intermittent operation and large-scale continuous production and has a relatively good commercial prospect.

The invention discloses an insect cell serum-free culture medium and a preparation process thereof. The insect cell serum-free culture medium comprises 11-16 g / L of amino acid, 5-15 g / L of carbohydrates, 7-12 g / L of inorganic salt, 0.01-0.2 g / L of vitamin, 3-10 g / L of a protein hydrolysate, 1-5 g / L of yeast powder and 15-35 g / L of a lipid emulsifier. The insect cell serum-free medium provided by the invention is clear in components, low in vitamin content, relatively low in cost and simple in preparation process, is beneficial to large-scale culture of insect cells, can greatly improve the insect cell culture efficiency, and has a good commercial prospect.

The invention relates to the technical field of DNA end group modification, and concretely relates to a DNA with an end group modified by a sulfur-containing reagent and a preparation method of the DNA with the end group modified by the sulfur-containing reagent. The method comprises the following two steps: preparing phosphoramidothiooctanol ester and preparing a DNA with the modified end group,wherein the step of preparing the phosphoramidothioctanol ester comprises the following steps: a reduction reaction is performed by taking lipoic acid as a raw material to obtain sulfur octanol, and an esterification reaction is performed to obtain the phosphoramidothiolate ester; and the step of preparing the DNA with the modified end group comprises the following steps: the prepared phosphoramidothiooctanol ester and DNA are subjected to synthesis to obtain the DNA with the end group modified by the sulfur-containing reagent. According to the invention, the lipoic acid is taken as the raw material, reduction is performed, and esterification is performed to obtain the phosphoramidothiooctanol ester, the route is simple and clear, the operation is convenient and quick, purification mannersof the intermediate and the product are simple, a large number of the DNA with the end group modified by the sulfur-containing reagent can be high efficiently prepared, and the method provides a controllable-cost simple and high-efficiency preparation method for sulfur-containing modification of 5' ends of DNAs.

The invention provides a microporous carbon fiber grafted polyaniline / CoNi2S4 composite material electrode. A preparation method of the microporous carbon fiber grafted polyaniline / CoNi2S4 composite material electrode comprises the following steps of preparation of microporous carbon fibers, preparation of microporous carbon fiber grafted polyaniline, preparation of a microporous carbon fiber grafted polyaniline / CoNi2S4 composite material, and preparation of the microporous carbon fiber grafted polyaniline / CoNi2S4 composite material electrode. The microporous carbon fiber grafted polyaniline / CoNi2S4 composite material electrode prepared by the method is simple in preparation process, low in cost and capable of being subjected to large-scale industrial production, and the obtained composite material electrode has very high specific capacitance and excellent recycling stability and is an excellent supercapacitor electrode material.

The invention provides a preparation method and application of three-dimensional dendritic crystal porous silicon, and belongs to the field of porous silicon preparation. The preparation method has the technical scheme that the preparation method comprises the following steps of Step 1, preparing a precursor alloy; Step 2, putting the precursor alloy, with the thickness being 5 mum to 500 mum, obtained in the Step 1 into corrosion liquid to be subjected to dealloying treatment for obtaining a dealloyed specimen; and Step 3, performing subsequent treatment to obtain a three-dimensional micrometer / nanometer dendritic crystal structure porous silicon material. The preparation method and the application of the three-dimensional dendritic crystal porous silicon have the beneficial effects thatcasting aluminum-silicon alloys commonly used in production are used as raw materials, so that the production cost is greatly reduced; meanwhile, the difficult problem that for powder materials, precursor alloy metallographic structures are difficult to control and to further regulate and control for finally preparing the structure and morphology of the porous silicon material can be solved; theprecursor alloy structure regulation and control and the experiment conditions are easy to control, so that the problem of industrialization bottleneck of low-cost energy storage can be solved; and good commercial prospects are realized.

The invention provides a low-temperature battery electrolytic solution based on an ionic liquid, a preparation method and application thereof. The electrolytic solution comprises an organic solvent, a lithium salt and an ionic liquid, wherein the ionic liquid is an imidazolium ionic liquid, a pyrrolyl ionic liquid or a pyridyl ionic liquid. The invention also provides a preparation method of the electrolytic solution. According to the invention, the low-temperature capacity retention rate and the cycle performance of the lithium ion battery containing the electrolytic solution are greatly improved.

The invention discloses a zinc soap-mannitol composite heat stabilizer for polyvinyl chloride (PVC) and a preparing method and application thereof. The heat stabilizer is a composition generated after zinc soap reacts with mannitol and is prepared from zinc soap, zinc soap-mannitol complex, mannitol and dehydrated mannitol. The raw material source is wide, the technology is simple and environmentally friendly, cost is low, production efficiency is high, and product performance is easy to control. The composite heat stabilizer is high in initial coloration property, well compatible with a PVC matrix, easy to disperse, capable of effectively improving the long-term heat stability of PVC, high in inside and outside lubricity, excellent in comprehensive performance, and capable of solving the problem of poor heat stability of existing environment-friendly PVC heat stabilizers.