Sensitization type immunochromatographic kit for sensitively detecting novel coronavirus antibody

Abstract

The invention relates to a sensitization type immunochromatographic kit for sensitively detecting a novel coronavirus antibody, which introduces a controllable copper growth technology to amplify theoptical signal intensity of a colloidal gold probe of a detection line on the basis of a colloidal gold immunochromatographic test strip so as to realize high-sensitivity detection of an object to bedetected. The kit comprises the colloidal gold immunochromatography test strip and a copper growth solution, wherein the colloidal gold immunochromatography test strip is formed by sequentially fixinga sample pad, a combination pad, a nitrocellulose membrane and absorbent paper on a PVC plastic bottom plate; the copper growth solution comprises a copper growth solution A and a copper growth solution B. The kit provided by the invention is simple to operate, high in sensitivity, good in stability, high in detection speed (20-30 minutes) and easy in result reading, does not need other auxiliaryinstruments and equipment, and can be used for on-site rapid ultra-sensitive detection and large-scale epidemiological investigation of clinical and emergency events.

Description

technical field [0001] The invention relates to the technical field of immunochromatography analysis, in particular to an enhanced immunochromatography kit for sensitive detection of novel coronavirus antibodies, the technical principle of which is a double-antigen sandwich method. Background technique [0002] Colloidal gold immunochromatographic test strips have been widely used in clinical diagnosis, food safety testing, and environmental monitoring because of their simplicity, speed, convenience, and naked-eye detection. Traditional colloidal gold immunochromatographic test strips mainly use colloidal gold with a particle size of 20-40nm as the signal probe. However, due to its small particle size, the colorimetric signal intensity is relatively weak, which leads to low detection sensitivity. The relatively low detection sensitivity limits its application in ultrasensitive detection to some extent. In recent years, with the rapid development of nanoscience and technolog...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is that the present invention aims at the existing defects of the existing colloidal gold immunochromatographic test strips due to its weak signal strength of 20-40nm colloidal gold probes resulting in low detection sensitivity. The controllable growth and deposition of copper on the surface of the needle significantly amplifies the optical signal intensity of the colloidal gold probe on the test strip detection line, providing a new technology to improve the detection sensitivity of the traditional colloidal gold immunochromatography method, and realize the ultra-low concentration target detection On-site rapid detection

Images