New method for sensitivity of supermolecular self-assembling mediated net-shaped nano-gold enhanced immunochromatographic test strip

Abstract

The invention provides a method for amplifying colloidal gold immunochromatographic colorimetric signal intensity. The method comprises the following steps: closing a gold-labelled antibody by using amantadine marked bovine serum albumin to obtain a gold-labelled antibody I; synthesizing colloidal gold nanoparticles by using beta-cyclodextrin to obtain a beta-cyclodextrin coated colloidal gold solution; after adding a to-be-tested sample into a system which executes double-antibody sandwich colloidal gold immunochromatography by using the gold-labelled antibody I, adding the beta-cyclodextrincoated colloidal gold solution; and adding a mixed solution of 5,10,15,20-tetra(4-carboxyl phenyl)porphyrin and the beta-cyclodextrin coated colloidal gold solution into the system repeatedly for manytimes. According to the method, net-shaped nano-gold is formed by utilizing supermolecular self-assembling principle mediation and circular signal amplification is realized, so that the developing intensity of the god nanoparticles on a detection line and a quality control line is greatly improved and the aim of performing on-site rapid detection on a target detection object with ultralow concentration can be fulfilled. The invention also provides a high-sensitivity kit applied to colloidal gold immunochromatography.

Description

technical field [0001] The invention relates to the technical field of immunochromatography analysis, and further relates to a method for amplifying the colorimetric signal intensity in colloidal gold immunochromatography and the improvement of test strips, in particular to a method for enhancing immunity based on supramolecular self-assembly mediated network nano-gold A new approach to chromatographic sensitivity. Background technique [0002] Colloidal gold immunochromatographic test strips have been widely used in clinical diagnosis, food safety testing, and environmental monitoring because of their simplicity, speed, convenience, and naked-eye detection. Traditional colloidal gold immunochromatographic test strips mainly use colloidal gold with a particle size of 20-40nm as the signal probe. However, due to its small particle size, the colorimetric signal intensity is relatively weak, which leads to low detection sensitivity. The relatively low detection sensitivity lim...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the current defect of the existing colloidal gold immunochromatography test strip, which has low detection sensitivity due to the weak signal strength of the 20-40nm colloidal gold probe, and uses supramolecular self-assembly to mediate the formation of network nano-gold, Promote the continuous accumulation of colloidal gold nanoparticles on the test strip detection line, provide a new method to enhance the sensitivity of immunochromatography analysis, and realize the purpose of on-site rapid detection of ultra-low concentration target detection substances

Images