New method for sensitivity of supermolecular self-assembling mediated net-shaped nano-gold enhanced immunochromatographic test strip
An immunochromatography and signal intensity technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of weak signal intensity and low detection sensitivity, and achieve the effects of low reagent cost, good reproducibility, and enhanced detection sensitivity.
Active Publication Date: 2018-08-28
SHANGHAI THERANOSTICS BIOTECH CO LTD
View PDF9 Cites 14 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] The present invention aims at the current defect of the existing colloidal gold immunochromatography test strip, which has low detection sensitivity due to the weak signal strength of the 20-40nm colloidal gold probe, and uses supramolecular self-assembly to mediate the formation of network nano-gold, Promote the continuous accumulation of colloidal gold nanoparticles on the test strip detection line, provide a new method to enhance the sensitivity of immunochromatography analysis, and realize the purpose of on-site rapid detection of ultra-low concentration target detection substances
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0050] A test kit for colloidal gold immunochromatography, which includes colloidal gold immunochromatography test strips and colorimetric signal amplification reagents; as figure 1 Shown in middle A, described colloidal gold immunochromatography test paper strip comprises sample pad, binding pad, nitrocellulose (NC) membrane and water-absorbing paper, and described binding pad is provided with the gold-labeled antibody blocked by ADA-labeled BSA , the described nitrocellulose (NC) membrane is provided with detection line (T line) and quality control line (C line); described colorimetric signal amplification reagent comprises TCPP solution and β-CD coated colloidal gold solution .
[0051] The kit is prepared as follows:
[0052] 1. Preparation of colloidal gold immunochromatographic test strips
[0053] 1. Preparation of gold-labeled detection antibody
[0054] 1.1. Preparation of amantadine-labeled bovine serum albumin (ADA@BSA):
[0055] The coupling of amantadine and b...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
| Sensitivity | aaaaa | aaaaa |
Login to view more
Abstract
The invention provides a method for amplifying colloidal gold immunochromatographic colorimetric signal intensity. The method comprises the following steps: closing a gold-labelled antibody by using amantadine marked bovine serum albumin to obtain a gold-labelled antibody I; synthesizing colloidal gold nanoparticles by using beta-cyclodextrin to obtain a beta-cyclodextrin coated colloidal gold solution; after adding a to-be-tested sample into a system which executes double-antibody sandwich colloidal gold immunochromatography by using the gold-labelled antibody I, adding the beta-cyclodextrincoated colloidal gold solution; and adding a mixed solution of 5,10,15,20-tetra(4-carboxyl phenyl)porphyrin and the beta-cyclodextrin coated colloidal gold solution into the system repeatedly for manytimes. According to the method, net-shaped nano-gold is formed by utilizing supermolecular self-assembling principle mediation and circular signal amplification is realized, so that the developing intensity of the god nanoparticles on a detection line and a quality control line is greatly improved and the aim of performing on-site rapid detection on a target detection object with ultralow concentration can be fulfilled. The invention also provides a high-sensitivity kit applied to colloidal gold immunochromatography.
Description
technical field [0001] The invention relates to the technical field of immunochromatography analysis, and further relates to a method for amplifying the colorimetric signal intensity in colloidal gold immunochromatography and the improvement of test strips, in particular to a method for enhancing immunity based on supramolecular self-assembly mediated network nano-gold A new approach to chromatographic sensitivity. Background technique [0002] Colloidal gold immunochromatographic test strips have been widely used in clinical diagnosis, food safety testing, and environmental monitoring because of their simplicity, speed, convenience, and naked-eye detection. Traditional colloidal gold immunochromatographic test strips mainly use colloidal gold with a particle size of 20-40nm as the signal probe. However, due to its small particle size, the colorimetric signal intensity is relatively weak, which leads to low detection sensitivity. The relatively low detection sensitivity lim...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 陈小元黄小林熊勇华
Owner SHANGHAI THERANOSTICS BIOTECH CO LTD
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap