42results about How to "Reduce mutual contamination" patented technology

The invention discloses a PCR (Polymerase Chain Reaction) chip based on droplet array. The chip is obtained by taking a mono crystalline silicon sheet or a glass sheet as a base material and adopting standard photoetching as well as wet etching technique. The mono crystalline silicon sheet or the glass sheet is the base material of the chip. A SiO2 oxide layer and a silane layer generated by silanization on the surface of the SiO2 oxide layer are adhered to the base material successively. A hydrophilic pit array, which can be a droplet array and is formed by etching with photoresist technology, is disposed on the silane layer. A closed circular guardrail is arranged on the outer periphery of the hydrophilic pit array and the guardrail is connected with the silane layer in a sealed manner. The invention also discloses the application of the chip on real-time quantitative PCR detection and real-time quantitative isothermal amplification reaction detection. The detection system provided in the invention remarkably reduces the reaction volume and the detection sensitivity of the system fairly equals that of a commercialized quantitative PCR instrument. The detection system in the invention is easy for operation and has low cost, thus can be applied to commercialization.

The invention discloses a desert type anti-infrared camouflage fabric printing technology. The technological process comprises steps as follows: printing, baking, padding by a reduction liquid, rapid vat ageing, oxidizing, soaping, hot water washing, washing and drying. A high-temperature type disperse dye with good sublimation fastness and small cotton fiber staining and an anthraquinone indanthrene dye are adopted to perform one-paste printing with a double-phase method, a technology of thermosol and later reduction is adopted after drying, so that the color yield is increased, mutual staining is reduced, and dark and gloomy color and luster are avoided; the light transmittance of terylene is 100%, that is, the light reflectivity is zero, so that the colored light requirements are met by adjusting the use quantity of the disperse dye when the colored light approaches.

The invention discloses a separable mask with an internal arching strip. The separable mask comprises mask cloth and bands for fixing the mask cloth, and is characterized in that an annular interlayer is arranged on the inner side of the mask cloth, the inner circumference of the interlayer is separated from the mask cloth, the interlayer is provided with an annular connecting line for connecting the interlayer with the mask cloth, and the connecting line is located on the outer side of the portion, separated from the mask cloth, of the interior of the interlayer to accordingly form a groove with an inwards-opened opening; a pressing piece is arranged and matched with the groove in size and shape to accordingly enable a part of the edge of the pressing piece to be installed in the groove, and separable connection with the mask cloth or the interlayer is accordingly achieved; the pressing piece is provided with a strip-shaped body arching towards the region, surrounded by the groove and located in front of the mouth or the nose, of the mask cloth, and the mask cloth is ejected through the strip-shaped body from the region, in front of the mouth or the nose, of the inner side of the mask cloth. Materials can be saved, the air impermeability can be improved, the effective air inspiration breathable area of the mask can be increased, the comfort of the mask can be improved, and the separable mask is healthier.

The invention discloses a cultivation method for straw mushrooms. The method comprises the following steps: (1) preparing a culture medium; (2) preparing fungus package, bagging the culture medium, sterilizing, inoculating and culturing to obtain mycelium; (3) performing fruiting management: a, cutting the fungus package prepared in the step (2) from the middle part, putting the cut fungus package into a basket with the section facing upwards, and covering with a film; b, removing the film and spraying water 1-2 days later, and spraying water into the basket by 1-3 centimeters in water depth; (4) harvesting. According to the cultivation method for the straw mushrooms disclosed by the invention, the fungus package is cut transversely from the middle part, so that regular formation of a straw mushroom primordium on a section can stimulated; the fungus package is placed into the basket for performing fruiting management, so that greater convenience is brought to operation, and the working efficiency is increased; the basket is filled with water, so that the environmental moisture of a mushroom room can be well maintained, the water spraying workload is lowered, and the phenomena of atrophy and death of the mushrooms caused by direct spray of water onto compost are avoided; a method for regulating the moisture by filling the basket with water is easy to comprehend, and is convenient to popularize.

The invention discloses a heat-sensitive umbrella type packer for sublevel mining. The packer comprises a power module, a packing module and a mining module; the power module comprises a drilling machine, a power head and a pump; the packing module is provided with upper and lower level umbrella-like packing rod systems with the same structure, wherein the packing rod systems are respectively positioned on the lower boundary between a target mineral layer and an upper lying stratum and the upper boundary between the target mineral layer and a lower lying stratum; the drilling machine drives amovable central pipe with a double-layer structure in the mining module to move upwards through the power head, and further drives the upper and lower level umbrella-like packing rod systems in the packing module capable of being axially folded and radially expanded, so that the upper lying stratum and the lower lying stratum are packed; and by using the packer, deep mining and layered mining of natural resources such as oil shale and the like can be effectively packed, so that the mining purity of minerals is improved, and cross contamination among the reservoirs is reduced.

The invention discloses a detection method and a kit of transgenic soybean MON89788 transformation event. The method comprises the following steps: performing high temperature degeneration on a sample DNA (deoxyribonucleic acid), transcribing under RNA (ribonucleic acid) polymerase by using a primer for identifying MON89788 specificity region to generate RNA, performing NASBA (nucleic acid sequence based amplification) by using the above primer based on obtained RNA as a template; and finally detecting the amplification product. Without purchasing large instrument, the detection cost of the method disclosed by the invention is reduced, the detected product is easy to degrade, and the problem of cross contamination among samples is reduced.

The invention provides an aquaculture device which comprises a base plate and columns arranged on the base plate at intervals. Fixing grooves are formed in the columns in the length directions of thecolumns, side plate racks are installed in the fixing grooves and are hollow, outer filling grooves and inner filling grooves are formed in the side plate racks, glass is inserted into the outer filling grooves, and screen plates are inserted into the inner filling grooves. The aquaculture device is simple in structure and good in practicability, the columns and the side plate racks define small divided aquaculture areas, the aquaculture device is suitable for aquaculture of same types or different types or different sizes of fry, the aquaculture areas are divided by sealing plates, the sealing plates are rotatably connected to a fixing shaft, division of different aquaculture areas and water and oxygen circulation of the different aquaculture areas can be achieved by turning the sealing plates.

The present invention provides a battery pack heat exchange system and an electric vehicle, pertaining to the technical field of new energy sources. The battery pack heat exchange system comprises multiple heat exchange devices, a total water inlet pipe and a total water outlet pipe. The multiple heat exchange devices are arranged corresponding to multiple batteries in a battery pack one by one, and each heat exchange device comprises a water inlet and a water outlet. The multiple water inlets are all connected with the total water inlet pipe, and the multiple water outlets are all connected with the total water outlet pipe, so that the multiple heat exchange devices are connected in parallel. Each heat exchange device is arranged under each single battery, the multiple heat exchange devices are connected in parallel, and when the heat exchange is needed, each heat exchange device performs heat exchange independently, so that the mutual contamination between the heat exchange devices is reduced, and the heat exchange of the battery pack is more even. The electric vehicle comprises the battery pack heat exchange system. The battery pack heat exchange system and the electric vehiclecan solve the technical problem of uneven temperature control among single batteries in the battery pack.

The invention belongs to the field of biomedicines, relates to an adenovirus chip and an application thereof and particularly relates to an adenovirus chip containing a human full-length genome and an application of the adenovirus chip. The adenovirus chip provided by the invention is prepared by printing adenovirus particles containing human full-length genes on a microscope glass chip. High-concentration adenovirus particles are produced, and high-concentration adenovirus is printed on a nitrated fiber glass sheet or other substrates by using a chip microarrayer. The adenovirus chip provided by the invention can also be converted into a protein chip. The adenovirus chip provided by the invention can be applied to multiple different biomedical researches including living cell analysis (cell growth and differentiation, cell apoptosis, tissue specificity genetic expression and analysis of cells to toxicity and sensitivity of micromolecules) and can also be used for immunity analysis, enzymatic activity analysis and promoter/reporter gene analysis.

The invention discloses a creation method of a special soybean protein germplasm for processing. The method comprises steps as follows: abundant seed main storage protein 7S and 11S component subunit content variation genes in soybean germplasm resources are utilized for carrying out identification and selection by means of pyramiding breeding, crossbreeding and the like in combination with an SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method and a molecular identification means, and firstly, germplasms only containing a 7S component and germplasms only containing a 11S component are created; then hybridized combinations with a series of germplasms highly containing the 7S component and a series of germplasms highly containing the 11S components obtained by screening are configured respectively, a series of materials only containing and highly containing the 7S component and a series of materials only containing and highly containing the 11S component are obtained through selection and stabilization, and strains are cultivated; and nutritional characteristics, functionality and processing characteristics are evaluated respectively, and the strains are classified into special soybean materials suitable for different processed products, and therefore, high-quality special raw materials are provided for soybean processing and utilization.

The invention discloses a deep brine exploration and mining combination technology. The deep brine exploration and mining combination technology comprises a hole drilling step, a hole measuring step,a hole expanding step, a well forming step, a water-sealing step, a well washing step, a water pumping step and a brine extracting step, wherein a water seal in the water sealing step includes an in-pipe water seal and an outer-pipe water seal. The deep brine exploration and extraction combination technology has the advantages that the water-sealing effect is excellent, the well forming efficiencyis improved, the costs are reduced, and the exploration and extraction combination is realized.

The invention discloses a linear control four-channel rotating switching valve for automobile oiling. The linear control four-channel rotating switching valve comprises an oil inlet system, a linear control flow diversion control system, a rotating switching valve body and oil outlet pipes. The oil inlet system comprises an oil filler and an oil filling pipe. The linear control flow diversion control system is used for controlling a control line fixed to a rotating valve element under the combined action of the rotating valve element and a reset spring so that the flow diversion controlling action of a baffle can be achieved. A drainage hole channel is formed in the rotating valve element, the rotating valve element is wrapped by a layer of oil-leaking-preventing seal cover, and the outer end of the rotating valve element is connected with a connecting rod and a handle. The bottom of each fixing valve shell is connected with the two oil outlet pipes. The linear control four-channel rotating switching valve is used for an automobile which is provided with four fuel oil tanks and controlling the switching among the oil tanks in the oiling process and can prevent cross pollution of oil gas volatilization among the oil tanks.

The invention discloses an electromagnetic type four-channel rotary switching valve for automobile refueling. The electromagnetic type four-channel rotary switching valve comprises an oil inlet system, baffles, an electromagnetic controller, standing valve shells, a rotary valve element, a flow diversion control valve body and oil outlet pipes. The baffles comprise the left baffle and the right baffle, and the top of the left baffle and the top of the right baffle are each provided with a reset spring. The electromagnetic controller is composed of a power source, an electromagnet, static contacts, movable contacts and armatures, wherein the static contacts are inlaid on the standing valve shells, the movable contacts are inlaid on the valve element, and the armatures are arranged at the bottoms of the baffles. A drainage pore passage is arranged inside the rotary valve element, and the rotary valve element is covered with a layer of oil leakage prevention seal sleeve; the outer end of the rotary valve element is connected with a connecting rod and a handle; the bottom of each standing valve shell is connected with two oil outlet pipes. The electromagnetic type four-channel rotary switching valve for automobile refueling aims at the automobile carrying four fuel oil tanks, performs control over switching among the oil tanks in the refueling process and can prevent mutual contamination caused by oil gas volatilization among the oil tanks.

The invention relates to a self-extinguishing flame-retardant cotton fabric and a preparation method and application thereof. According to the self-extinguishing flame-retardant cotton fabric, 5-20 flame-retardant unit layers are deposited on the surface of a cotton fabric; each flame-retardant unit layer includes a silane coupling agent layer, a sodium alginate layer, a silane coupling agent layer and an ammonium polyphosphate layer in sequence or a silane coupling agent layer, an ammonium polyphosphate layer, a silane coupling agent layer and a sodium alginate layer in sequence. The preparation method comprises the steps that the cotton fabric is treated with a silane coupling agent, a sodium alginate solution and an ammonium polyphosphate solution are sprayed alternate with the silane coupling agent, layer-by-layer deposition on the surface of the cotton fabric is conducted, one flame-retardant unit layer is formed, and the operation is repeated to obtain the self-extinguishing flame-retardant cotton fabric. The preparation method is environmentally friendly and simple in operation, and raw materials are saved; the char yield of the coating cotton fabric is high, and the cottonfabric can be self-extinguished after being taken away from fire; the self-extinguishing flame-retardant cotton fabric is wide in application prospect and suitable for large-scaled production.

The invention relates to a method for rapidly extracting mitochondrial DNA and application and a related kit thereof. The method comprises the steps that a tissue sample or a blood sample with no red blood cell is dispersed; a lysis buffer is added into the dispersed cell sample, the lysis buffer is a water solution containing Tris-HCl and TritonX-100, the molar concentration of Tris-HCl is 19-21 mM, and the pH of the water solution is 8.7-8.9; the volume concentration of TritonX-100 is 0.09-0.11%; or, the lysis buffer is a water solution containing Tris-HCl and SDS, the molar concentration of Tris-HCl is 19-21 mM, and the pH of the water solution is 8.7-8.9; the mass concentration of the SDS is 0.09-0.11%; a supernate is separated out, and the mitochondrial DNA of the sample is obtained through extraction. By adopting the method, the mitochondrial DNA capable of being adopted as an amplification template can be obtained in a short period of time.

The invention provides an aquaculture device. The device comprises a bottom plate and upright columns arranged on the bottom plate at equal intervals; a fixing groove is formed in each upright columnin the length direction of the upright column; side plate frames are arranged in the fixing grooves, the side plate frames are hollow, and outer filling grooves and inner filling grooves are formed inthe side plate frames; glass is inserted into the outer filling grooves, and mesh boards are inserted into the inner filling grooves. The device is simple in structure, the practicability is high, and the upright columns and the side plate frames define small segmented breeding areas to be suitable for breeding of different types of fry; the glass can be inserted into the outer filling grooves inthe side plate frames so that breeding areas with different fry can be separated; the mesh boards can be inserted into the inner filling grooves in the side plate frames so that breeding areas with same fry can be separated, the growth space of the fry can be balanced, the breeding structure can be adjusted, and the breeding benefit is improved.

The invention provides a color silica gel cutting and transferring device which comprises a base discharging plate, a cutting and transferring tool and a chroma key jig plate. The base discharging plate is transversely provided with multiple longitudinal strip-shaped grooves allowing color blocks to be put therein. The groove wall of each longitudinal strip-shaped groove is provided with multiple split groove openings at equal intervals. The cutting and transferring tool comprises handles, pressing plates and cutters. The handles are in a strip shape and arranged above the pressing plates and connected with the pressing plates through elastic columns. The cutters are fixedly arranged below the pressing plates. The front ends of the cutters are correspondingly provided with multiple protruding portion tool bits corresponding to longitudinal strip-shaped grooves. The rear ends of the cutters are in the same plate. Multiple transfer needles are correspondingly arranged on the protruding portion tool bits. The pressing plates are provided with through holes matched with and corresponding to the transfer needles. The chroma key jig plate is provided with multiple chroma key containing positions corresponding to the longitudinal strip-shaped grooves in position one by one. The color silica gel cutting and transferring device is clever in structure and high in practicability, the yield is increased, production cost is lowered, and the yield of products is increased.

The invention relates to an environment-friendly pressing belt type continuous ship loader comprising a ship loader main vehicle and a ship loader tail vehicle, wherein the discharge end of the ship loader tail vehicle is connected with the feeding end of the ship loader main vehicle, the feeding end of the ship loader tail vehicle is connected with a ground conveyer, and the ship loader tail vehicle is a pressing belt conveyer; the pressing belt conveyer comprises a truss, and an upper-layer belt and a lower-layer belt which are laminated to each other, and the upper-layer belt and the lower-layer belt are arranged on a roller rack fixedly connected with the truss. The ship loader disclosed by the scheme adopts the pressing belt conveyer, can realize conveying with a dip angle of 0-90 degrees, and can reduce the length of the tail vehicle of the ship loader to the greatest extent, reduce equipment arrangement area and shorten feeding stroke; in addition, by adopting the pressing belttype conveyer, sealed conveying of material is realized, and mutual pollution between flying dust and material and environment is alleviated.

The invention relates to an indoor positive and negative pressure control system. At least one indoor air inlet fan and at least one indoor exhaust fan are arranged indoors, a corresponding exhaust port is formed in indoor space where the indoor air inlet fan is located, the indoor air inlet fan is used for guiding outdoor air into the indoor space, and the indoor exhaust fan is used for guiding indoor air to outdoor space. The indoor positive and negative pressure control system further comprises an indoor air quality detection module and a control module, the indoor air quality detection module is arranged near the exhaust port, and the control module is used for controlling starting and shutdown of the indoor air inlet fan and the indoor exhaust fan according to data obtained by the indoor air quality detection module. By controlling the indoor air inlet amount and the indoor air outlet amount, the specific flow direction of indoor air is achieved, and mutual pollution of indoor pollutants is reduced.