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Application of cry2Ah-vp gene in armyworm resisting

A kind of armyworm and gene technology, applied in the application field of cry2Ah-vp gene in anti armyworm

Active Publication Date: 2019-05-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that Cry2Ah protein can inhibit the body weight of armyworms, but has no lethal effect (Liu Chen, Chen Lin et al., Evaluation of the insecticidal activity of four Bt proteins against six important Lepidoptera pests, Chinese Journal of Biological Control, 2017 , 33(6)774-779)

Method used

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  • Application of cry2Ah-vp gene in armyworm resisting
  • Application of cry2Ah-vp gene in armyworm resisting
  • Application of cry2Ah-vp gene in armyworm resisting

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Experimental program
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Effect test

Embodiment 1

[0028] 1.1 Materials

[0029] Plant materials Bt cry2Ah gene insect-resistant corn material (preserved by our laboratory), corn inbred line Zheng 58.

[0030] The eggs of armyworm and corn borer were purchased from Beijing Meiyan Agricultural Technology Co., Ltd.

[0031] The tested pesticide glufosinate-ammonium (water formulation, active ingredient content: 200 g / L) was purchased from Zhejiang Yongnong Bioscience Co., Ltd.

[0032] BAR rapid detection test strips were purchased from Beijing Ao Chuang Jinbiao Biotechnology Company.

[0033] 1.2 Genetically modified corn

[0034] 1.2.1 Construction of plant expression vectors

[0035] In order to transfer the modified mcry2Ah (SEQ ID No. 4) and mcry2Ah-vp (SEQ ID No. 2) genes into maize, two plant expression vectors were constructed. The backbone vector of the plant expression vector is the commercial vector pCAMBIA3300, and the screening marker gene phosphinothricin (PPT) already exists on the pCAMBIA3300 vector, which can ...

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Abstract

The invention relates to application of a cry2Ah-vp gene in armyworm resisting. The applicant inserts a proline behind No. 354 bit valine of cry2Ah and a cry2Ah1-vp mutant shown as the SEQ ID No. 1 isobtained accordingly. The cry2Ah-vp gene is obtained after codon optimization, the cry2Ah-vp gene is transplanted into maize with an agrobacterium-mediated method, and T0 generation transgenic maizeis obtained. The experimental result shows the cry2Ah-vp gene can be normally transcribed into different tissues of the maize, the expression quantity of the cry2Ah-vp protein in all parts of the maize is the maximum in the six-leaf stage, the leaf expression quantity is 2.20 mu g / g (fresh weight), and all parts have higher expression in the tasseling stage and the filling stage. The insect resisting experiment result shows that the transgenic maize has good insecticidal activity to the armyworms, and the armyworms are all dead after inoculated to the maize three days; the cry2Ah-vp gene has aremarkable growth inhibition effect to the ostrinia nubilalis larvae.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of cry2Ah-vp gene in anti-myxoplasma. Background technique [0002] Corn is an important food crop as well as an important feed and industrial raw material, but due to the occurrence of insect pests, the annual corn yield is reduced by about 7%, and in severe cases it can reach 20%. In 1996, transgenic insect-resistant crops began to be commercially planted. The widespread planting of Bt crops has effectively prevented pests, reduced the use of pesticides, and reduced production costs. It has no adverse effects on the environment, beneficial insects, humans and animals [Bates S L ,Zhao J Z,Roush R T,Shelton A M.Insect resistance management in GM crops:past,present and future.Nature Biotechnology,2005,23:57-62.Estruch J J,Carozzi NB,Desai N,Duck N B,Warren G W,Koziel MG. Transgenic plants: an emerging approach to pest control. Nature Biotechnology, 1997, 15:137-141.]....

Claims

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Application Information

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IPC IPC(8): C07K14/325C12N15/31C12N15/82A01H5/00A01H6/46
Inventor 郎志宏李圣彦张杰汪海李梦桃
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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