157 results about "Glufosinate" patented technology

The invention discloses a copper-based nano-enzyme, a preparation method thereof and application of the copper-based nano-enzyme in organophosphorus pesticide detection, and belongs to the technical field of pesticide residue detection. The copper-based nano-enzyme has a netted-like structure, a high specific surface area and many active sites, the valence state of a copper element is zero-valence Cu, Cu < + > and Cu < 2 + >, the copper-based nano-enzyme has high peroxidase-like activity and laccase-like activity, the detection limit is low, and glyphosate can be sensitively detected; and glyphosate, glufosinate-ammonium and isazofos can be well distinguished from six organophosphorus pesticides with medium concentration, and the method has high selectivity. The nano enzyme provided by the invention is prepared from ammonium citrate and copper nitrate in an N, N-dimethylformamide (DMF) solution by using a solvothermal method; the preparation process of the nano enzyme is simple.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.

Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.

The invention belongs to the technical field of molecular biology, and particularly relates to a transgenic soybean event LP689-2 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP689-2, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from the transgenic soybean event LP689-2. A representative sample of a seed of the transgenic soybean event LP689-2 is preserved in the China Center for Type Culture Collection on June 28, 2025, and the preservation number is CCTCC NO: P202519. The transgenic soybean event LP689-2 has the character of tolerance to quadruple herbicides containing 2, 4-D, HPPD inhibitors, glufosinate-ammonium and glyphosate, meanwhile, the soybean yield is not reduced, and the related detection method can be used for rapidly, accurately and stably identifying the existence of plant materials derived from the transgenic soybean event LP689-2.

The present invention relates to a method for post-emergent weed control, which comprises applying an effective amount of a composition comprising herbicide A (component A), glufosinate (component B) and optionally herbicide C (component C) to emerged weeds, or an area, where weeds are growing.

The invention relates to a corn transformation event MN85N-E6 nucleic acid molecule and a detection method. The corn transformation event MN85N-E6 nucleic acid molecule comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The corn transformation event MN85N-E6 provided by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event MN85N-E6 or not.

The present application relates to the field of pesticides, and specifically discloses a pesticide composition containing fluazifop-butyl and glufosinate-ammonium, and a preparation method and application thereof. The pesticide composition comprises the following raw materials: fluazifop-butyl, glufosinate-ammonium, a dispersant, an emulsifier, an anti-settling agent and vegetable oil; wherein the emulsifier is a mixture of sodium salt of sulfonated styrene-maleic anhydride copolymer and hydroxylated lecithin; and the preparation method comprises the following steps: mixing and stirring the vegetable oil, the emulsifier and the anti-settling agent to obtain an oil phase, then adding fluazifop-butyl and glufosinate-ammonium, mixing and stirring, adding the dispersant, and then performing homogenization and grinding to obtain a dispersible oil suspension type pesticide composition; the present application also discloses the use of the above pesticide composition for weeding. The present application has the characteristics of a pesticide reagent containing fluazifop-butyl and glufosinate-ammonium at the same time, which can be directly diluted with water when used without the need for fresh preparation, while ensuring its efficacy and being simpler and more convenient to operate.

The present invention is a herbicide composition containing the following components (A), (B) and (C), wherein a mass ratio between the content of component (B) and the content of component (C), (C) / (B), is 0.010 or more and 0.18 or less:component (A): a compound selected from glufosinate and a salt thereof;component (B): a compound represented by the following general formula (B-I):R1O(R2O)nSO3−M+  (B-I)wherein R1 is a hydrocarbon group with 8 or more and 24 or less carbons, R2 is an alkanediyl group with 2 or more and 4 or less carbons, n is an average number of added moles and 0 or more and 30 or less, and M+ is a counter ion; andcomponent (C): an aromatic sulfonate salt having an aliphatic hydrocarbon group.

The present invention relates to a method for post-emergent weed control, which comprises applying an effective amount of a composition comprising herbicide A (component A), glufosinate (component B) and optionally herbicide C (component C) to emerged weeds, or an area, where weeds are growing.

The invention provides a mutant derived from Clostridium difficile glutamate dehydrogenase and an application of the mutant in preparation of L-glufosinate-ammonium. The glutamate dehydrogenase mutant is a glutamate dehydrogenase single site mutant, a double site mutant or a multi-site mutant. The L-glufosinate-ammonium can be used for preparing L-glufosinate-ammonium from L-glufosinate-ammonium from L-glufosinate-ammonium from L-glufosinate-ammonium from L-glufosinate-ammonium from L-glufosinate-ammonium. The invention effectively solves the problem that glutamate dehydrogenase shows inactivity or low activity in biological catalytic conversion taking 2-carbonyl-4-(hydroxymethylphosphonyl) butyric acid as a substrate, and provides a chemical method for continuously synthesizing the substrate 2-carbonyl-4-(hydroxymethylphosphonyl) butyric acid by a whole-process microchannel reactor.

The present invention relates to a mutant gene of rice OsSPL10 gene and its application. The nucleotide sequence of the mutant gene is shown in SEQ IN NO.1. This mutant gene can endow rice with resistance to glufosinate herbicide. The present invention uses the EMS chemical mutagenesis technology to treat the rice variety "Yangxian 9B", successfully induces the mutation of the OsSPL10 gene, and this mutation causes the 225th amino acid in the amino acid sequence of the encoded protein to change from cysteine to tyrosine. At the same time, the present application develops a KASP molecular marker for this mutation site. Through molecular marker detection, field glufosinate resistance screening and verification, it is proved that rice with this mutant gene can improve glufosinate resistance. This gene has significant application potential in agricultural production, can reduce the use of herbicides and improve crop yield and quality.

Provided is transgenic maize event QY2569-42, and further provided are a nucleic acid sequence and detection method for detecting maize plant QY2569-42. The nucleic acid sequence of the maize plant comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 11 and / or SEQ ID NO: 12 or a nucleic acid sequence complementary thereto. The maize plant QY2569-42 has good resistance to Lepidopteran insects and good tolerance to glufosinate ammonium herbicides, and has no effect on the yield; and the detection method can accurately and rapidly identify whether the DNA molecule of transgenic maize plant QY2569-42 is contained in a biological sample.

The present invention relates to an enzymatically catalyzed method for producing L-glufosinate or a phosphoester thereof. The method includes reacting an activated L-homoserine HA with a substrate S selected from methylphosphinic acid and esters thereof. Sulfhydrylase enzyme E1 is used for the enzymatic catalysis. The invention makes accessible new substrates for the enzymatic production of L-glufosinate and its phosphoesters.

The present invention relates to specific herbicide combinations comprising (i) L-glufosinate and / or salts thereof and (ii) pyraflufen-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said specific herbicide combinations. The present invention also relates to the use of said specific herbicide combinations and compositions comprising said specific herbicide combinations in the field of agriculture, for controlling harmful plants or undesired plant growth, as well as to corresponding methods.

The invention discloses a recombinant D-amino acid oxidase mutant, a coding gene and application thereof, and belongs to the technical field of enzyme engineering. A D-amino acid oxidase mutant is constructed, the catalytic activity of enzyme is greatly improved, and the specific enzyme activity can be improved to 54.32 U / g. The constructed mutant is used for catalyzing D / L-glufosinate-ammonium, under the participation of glutamate dehydrogenase and ammonium formate dehydrogenase, a catalytic reaction can be carried out under the 1M substrate concentration, the catalytic conversion rate is 99%, the yield is 97%, and the e.e. Value is gt; and the effect is 99.9%.

The invention belongs to the technical field of herbicides, and particularly relates to a herbicide for preventing and removing weeds in transgenic corn and soybean fields. A herbicide for preventing and removing weeds in transgenic corn and soybean fields comprises an active component and an auxiliary component, and the active component is formed by compounding glyphosate or glufosinate-ammonium and Epyrifenacil according to the mass ratio of (1-80): (80-1). After glyphosate or glufosinate-ammonium is compounded with Epyrifenacil, the control effect on eleusine indica, abutilon fiber and the like can be improved, and the herbicide controlling spectrum is expanded. Besides, the herbicide active component is formed by compounding two weeding active components according to a certain proportion, compared with a single-action-mechanism herbicide, the herbicide resistance evolution of weeds can be effectively controlled, the service life of the herbicide can be prolonged, and then important support is provided for scientific and effective prevention and control of the weeds.

The present invention relates to the technical field of pesticide detection, and specifically relates to a method for determining the pesticide residue content in tea, including sample pretreatment, sample purification, matrix calibration, and correction of the detection results based on a calibration curve. By optimizing the sample pretreatment, purification, and detection steps, this method can effectively reduce the matrix effect of tea and improve the accuracy and reliability of the determination of pesticide residue content in tea. At the same time, the present invention overcomes the problem that glyphosate and glufosinate cannot be jointly tested with other pesticides, and improves the convenience of pesticide detection while ensuring the accuracy of pesticide detection. The method described in the present invention has a reasonable operation process, can quickly and accurately determine the pesticide residue content in tea, and provides strong technical support for the supervision of tea quality and safety, quality control, and consumer health protection.

The present invention relates to herbicidal mixtures and their methods and uses for controlling undesirable vegetation in soybeans, wherein the herbicidal mixtures comprise L-glufosinate and and at least one herbicidal compound II selected from clethodim, cycloxydim, fenoxaprop, fenoxaprop-ethyl, fenoxaprop-P, fenoxaprop-P-ethyl, fluazifop, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl, haloxyfop, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-methyl, propaquizafop, quizalofop, quizalofop-ethyl, quizalofop-tefuryl, quizalofop-P, quizalofop-P-ethyl, quizalofop-P-tefuryl, sethoxydim, chlorimuron, chlorimuron-ethyl, nicosulfuron, oxasulfuron, thifensulfuron, thifensulfuron-methyl, tritosulfuron, imazamox, imazamox ammonium, imazapic, imazapic ammonium, imazapyr, imazapyr isopropylammonium, imazaquin, imazethapyr, imazethapyr ammonium, cloransulam, cloransulam-methyl, diclosulam, flumetsulam, atrazine, metribuzin, linuron, monolinuron, bentazone, bentazone-sodium, acifluorfen, acifluorfen-sodium, carfentrazone, carfentrazone-ethyl, flumiclorac, flumiclorac-pentyl, flumioxazin, fluthiacet, fluthiacet-methyl, fomesafen, lactofen, oxyfluorfen, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazin, norflurazon, picolinafen, bicyclopyrone, clomazone, isoxaflutole, mesotrione, pyrasulfotole, tembotrione, topramezone, fenquinotrione, glyphosate, glyphosate-isopropylammonium, glyphosate-potassium, glyphosate-trimesium (sulfosate), pendimethalin, trifluralin; acetochlor, alachlor, butachlor, dimethenamid, dimethenamid-P, metazachlor, metolachlor, S-metolachlor, pethoxamid, pyroxasulfone, 2,4-D and its salts and esters, clopyralid and its salts and esters, dicamba and its salts and esters, quinclorac, quinclorac dimethylammonium, quinmerac, diflufenzopyr, diflufenzopyr-sodium, tepraloxydim, cinmethylin and other compounds.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eJ and a detection method. The nucleic acid molecule of the rice RN85zN-eJ comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The rice transformation event RN85zN-eJ has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eJ or not.

The present invention refers to a modified glutamate dehydrogenase (GluDH). In particular the modified GluDH of the present invention has an increased activity for catalyzing the reaction of 4-(hydroxymethylphosphinyl)-2-oxobutanoic acid (PPO) and an amino donor to generate L-glufosinate and / or an improved dynamic property. The present invention also refers to the polynucleotide encoding the modified GluDH of the present invention, the vector and host cell for expressing the modified GluDH of the present invention and the method of producing L-glufosinate with the modified GluDH and host cell of the present invention.

The present invention relates to herbicide mixtures comprising isoxaflutol, pyroxasulfone, and an imidazolinone herbicide. The herbicide mixtures may comprise at least one additional herbicide selected from the group consisting of glyphosate and its salts, glufosinate and its salts, L-glufosinate (glufosinate-P) and its salts, dicamba and its salts and esters, 2,4-D and its salts and esters, bicyclopyrone, phenquinetrione, mesotrione, tembotrione, tolpyralate, and topramezone. Furthermore, the invention relates to methods and uses for applying the herbicide mixtures, particularly for controlling unwanted vegetation in soybean and cotton crops.

The invention provides a herbicide composition, a herbicide as well as a preparation method and application of the herbicide, and belongs to the technical field of agricultural herbicides. The composition provided by the invention comprises (1-hydroxypropane-1, 3-diyl) bis (methylphosphonic acid) and glufosinate-ammonium, and the weight ratio of the (1-hydroxypropane-1, 3-diyl) bis (methylphosphonic acid) to the glufosinate-ammonium is 20: 1-50: 1. The composition can be used for controlling weeds, especially weeds which have resistance to glufosinate-ammonium. The (1-hydroxypropane-1, 3-diyl) bis (methylphosphonic acid) and glufosinate-ammonium are compounded, a remarkable synergistic effect is shown, the prevention and treatment effect of glufosinate-ammonium is improved, the increasingly prominent resistance problem in use of a single glufosinate-ammonium agent can be effectively solved, and the weeding composition can be widely applied to the technical field of agricultural weeding.

The present disclosure relates to a method for controlling unwanted vegetation / weeds. More particularly, the present disclosure relates to a method for controlling Brachiaria genus weeds with synergistic herbicidal combinations comprising L-glufosinate, salts, esters, or combinations thereof and at least one additional herbicide.

A rice herbicide resistance genetic locus and its application. This application discloses a method for cultivating glufosinate-resistant plants, comprising reducing or knocking out the expression of the SPL10 gene in plants, thereby cultivating plants with stronger glufosinate resistance than wild-type control plants.

The invention discloses a butyl-containing waste liquid treatment method, and belongs to the technical field of chemical engineering, the method comprises the following steps: converting 1-chlorobutane into n-butyl alcohol through alkaline resin, hydrolyzing n-butyl acetate in an alkaline reagent to obtain n-butyl alcohol, and then recovering high-purity n-butyl alcohol through extractive distillation; according to the method, the n-butyl alcohol is efficiently recovered from the waste liquid and returns to the front end again to serve as a raw material to be input, and the resin and the extraction agent used in the method can be recycled; and the treated waste liquid is simple to treat and low in cost, so that the production cost of preparing glufosinate-ammonium is reduced, the pollution to the environment is reduced, and the method has very high industrial application value.

20990055_1 (GHMatters) P45843AU01 Abstract Herbicidal mixtures comprising L-glufosinate or its salt and at least one VLCFA inhibitor The present invention relates to herbicidal mixture comprising L-glufosinate or its salt and at 5 least one VLCFA inhibitor. The invention furthermore relates to a method for controlling unde- sirable vegetation in burndown programs, in industrial vegetation management and forestry, in vegetable and perennial crops and in turf and lawn. Abstract Herbicidal mixtures comprising L-glufosinate or its salt and at least one VLCFA inhibitor 5 The present invention relates to herbicidal mixture comprising L-glufosinate or its salt and at least one VLCFA inhibitor. The invention furthermore relates to a method for controlling unde- sirable vegetation in burndown programs, in industrial vegetation management and forestry, in vegetable and perennial crops and in turf and lawn. 20990055_1 (GHMatters) P45843AU01 20 24 20 48 66 15 J ul 2 02 4 A b s t r a c t 2 0 2 4 2 0 4 8 6 6 1 5 J u l 2 0 2 4

This invention belongs to the field of plant breeding technology and relates to the transgenic soybean event JK1013-3 and its detection method. This invention provides a nucleic acid molecule for detecting the transgenic soybean event JK1013-3. The sequence of the nucleic acid molecule is shown in one or more of SEQ ID NO:1-2, SEQ ID NO:3-4, SEQ ID NO:5, or their complementary sequences. The nucleic acid molecule originates from the transgenic soybean event JK1013-3, and soybean seeds containing the transgenic soybean event JK1013-3 have been deposited at the China Center for Type Culture Collection (CCTCC) with the accession number CCTCC NO:P202529. Soybean plants containing the transgenic soybean event JK1013-3 of this invention have the following advantages: they contain the human milk β-casein gene and can stably express human milk β-casein; they are tolerant to the commonly used commercial herbicide glufosinate; they do not reduce soybean yield; and they can be used with molecular markers to track transgenic insertion fragments in the breeding population and its offspring. Simultaneously, the detection method provided by this invention can rapidly, accurately, and stably identify the presence of plant material derived from the transgenic soybean event JK1013-3.

To provide a herbicidal mixture exhibiting higher activity in burndown programs, industrial vegetation management and forestry, vegetable and perennial crops, and turf and lawn.SOLUTION: (a) L-glufosinate and / or its salts as compound I; and (b) at least one herbicidal compound II which is cinmethylin, wherein L-glufosinate comprises more than 70% by weight of the L-enantiomer and wherein the weight ratio of compound I to compound II is from 1000:1 to 1:500.SELECTED DRAWING: None

The present invention primarily relates to the use of compositions comprising L-glufosinate and / or salts thereof in a glufosinate tolerant field crop in foliar application, wherein the compositions contain less than 5 mol.-% of D-glufosinate and / or salts thereof, based on the total amount of L-glufosinate and salts thereof, under certain environmental conditions to achieve an increase in the control of harmful plants and / or a reduction of phytotoxicity in the tolerant field crop. The present invention also relates to according methods of treating a tolerant field crop using the mentioned compositions.