Determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food
A technology of aminomethylphosphoric acid and a determination method, applied in the field of determination of pesticide residues, can solve problems such as unavailability, and achieve the effects of simple operation, good repeatability and high sensitivity
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[0047] Example 1: Detection of glufosinate-ammonium, glyphosate and aminomethyl phosphate residues in carrots
[0048] (1) Sample pretreatment
[0049] Weigh 10.0 g of the homogenized carrot sample into a 50 mL stoppered plastic centrifuge tube, add 10 mL of aqueous methanol, vortex for 1 min, and centrifuge at 5000 r / min for 5 min. After centrifugation, take 2 mL of the supernatant extract into a centrifuge tube containing 100 mg of C18, vortex for 1 min, and centrifuge at 5000 r / min for 3 min. After the supernatant was passed through a 0.22 μm filter membrane, it was derivatized.
[0050] (2) Preparation of standard working solution
[0051] Weigh 25±0.1mg of standard substance into a 25mL plastic volumetric flask, dissolve with acetonitrile, and dilute to get 1000.0μg / mL standard stock solution; pipette 1.0mL each of glufosinate-ammonium, glyphosate and aminomethylphosphoric acid standard stock solution Place in a 100mL plastic volumetric flask, and dilute to volume with...
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