Determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food

A technology of aminomethylphosphoric acid and a determination method, applied in the field of determination of pesticide residues, can solve problems such as unavailability, and achieve the effects of simple operation, good repeatability and high sensitivity

Inactive Publication Date: 2014-05-28
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Application Information

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Problems solved by technology

[0009] Another object of the present invention is to provide a sample pretreatment method to make up for the deficiency that the existing internationally recognized QuEChERS method cannot be used for the detection of the three pesticide residues of glufosinate, glyphosate and aminomethyl phosphate

Method used

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  • Determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food
  • Determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food
  • Determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food

Examples

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Embodiment 1

[0047] Example 1: Detection of glufosinate-ammonium, glyphosate and aminomethyl phosphate residues in carrots

[0048] (1) Sample pretreatment

[0049] Weigh 10.0 g of the homogenized carrot sample into a 50 mL stoppered plastic centrifuge tube, add 10 mL of aqueous methanol, vortex for 1 min, and centrifuge at 5000 r / min for 5 min. After centrifugation, take 2 mL of the supernatant extract into a centrifuge tube containing 100 mg of C18, vortex for 1 min, and centrifuge at 5000 r / min for 3 min. After the supernatant was passed through a 0.22 μm filter membrane, it was derivatized.

[0050] (2) Preparation of standard working solution

[0051] Weigh 25±0.1mg of standard substance into a 25mL plastic volumetric flask, dissolve with acetonitrile, and dilute to get 1000.0μg / mL standard stock solution; pipette 1.0mL each of glufosinate-ammonium, glyphosate and aminomethylphosphoric acid standard stock solution Place in a 100mL plastic volumetric flask, and dilute to volume with...

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Abstract

The invention discloses a determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food. The determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food comprises the following steps: in the sample pretreatment, firstly, extracting glufosinate, glyphosate and aminomethyl phosphoric acid in a food sample by a methanol water solution in sample pretreatment; then purifying an extracting solution by selecting a C18 dispersive solid-phase extraction agent; and finally, filtering, deriving, and determining three compounds qualitatively and quantitatively synchronously by combining with high performance liquid chromatography-tandem mass spectrum. According to the method provided by the invention, the operation is simple and convenient, and the reproducibility is good, and impurities in a sample extraction solution can be removed effectively; the pretreatment is the supplement and improvement of the existing QuEChERS method; the recycling rate can reach 70.5%-91.2%; the relative standard deviation is 4.5%-7.83%; the limit of quantification is 50micro-g / kg; the determination method can completely meet the technical requirement on safety detection of China, European Union, America and Japan, and can provide powerful technical support for food safety and export trade healthy development for people of China.

Description

technical field [0001] The invention belongs to the technical field of determination of pesticide residues, in particular to a method for determination of glufosinate-ammonium, glyphosate and aminomethylphosphoric acid residues. Background technique [0002] Glyphosate (PMG) is a broad-spectrum herbicide developed by Monsanto in the 1970s. It is the organophosphorus pesticide with the strongest herbicidal activity. For various parts of plants, it can control more than 40 families of plants such as monocots and dicotyledons, annuals and perennials, herbs and shrubs. It is widely used because of its low drug cost, strong conductivity, good drug effect, and excellent environmental compatibility. [0003] Aminomethyl phosphonic acid (AMPA) is the main metabolite of glyphosate in plants, soil and water. [0004] Glufosinate (glufosinate, GLUF) is a new herbicide developed by Hearst in the 1980s. For fruit trees, row crops, vegetables and non-arable lands grown in wide rows. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘靖靖孙忠松崔淑华
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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