10 results about "Chemical labeling" patented technology

The invention relates to ribozyme for labeling biotin on target RNA (Ribonucleic Acid) as well as a screening method and application thereof. The RNA labeling technology is one of core tools for modern molecular biology research and clinical diagnosis. A specific marker is covalently linked to a target RNA molecule, so that accurate tracking, efficient detection and functional analysis of RNA can be realized. A biotin-streptavidin system becomes one of the technical routes which are most widely applied due to extremely high affinity and signal amplification capability of the biotin-streptavidin system. At present, common biotin-labeled RNA technical methods mainly comprise a chemical solid-phase synthesis method, an enzymatic method and a chemical labeling method. However, the methods have the limitations of poor site specificity, low efficiency, complex manipulation and the like in the use process. The engineering ribozyme for catalyzing RNA biotinylation is obtained by taking 12: 0 biotin coenzyme A as a biotin donor through an in-vitro screening technology. The ribozyme provided by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

The present application belongs to the technical field of analysis and identification of fermented food, and particularly relates to a method for distinguishing and identifying fermented food of different processes based on chemical isotope labeling liquid chromatography-mass spectrometry (CIL-LC-MS) technology analysis of carboxylic acid. In order to accurately and simply identify the type and content of carboxylic acid, and thus based on carboxylic acid panorama analysis, efficiently and conveniently distinguish and identify fermented food of different processes, the present application introduces CIL-LC-MS technology to analyze carboxylic acid and thus distinguish and identify fermented food of different processes. The technology can significantly enhance the response value and detection sensitivity of carboxylic acid through chemical labeling strategy, and effectively overcome the ion suppression interference of the matrix by using isotope internal standard, so as to realize accurate quantification. Combined with its inherent three-level identification process, the present application can efficiently and conveniently distinguish and identify fermented food of different processes.

The invention relates to the technical field of biological detection, in particular to a dry-state hydrogel-based plasma-Fabry-Perot cavity biological detector as well as a preparation method and application thereof. Compared with ELISA and other immunological detection methods depending on chemical tags and signal amplification, the method does not need a signal amplification process and shortens the detection time. The integrated plasma interlayer thickness sensing structure provided by the invention realizes direct signal reading after sample incubation is finished. According to the invention, the hydrogel is used as a sensing interlayer, and the hydrogel is used as a three-dimensional medium, so that a larger thickness change can be generated in a spatial scale. The biological detector can still keep relatively high specificity in a complex protein liquid phase environment (serum). The device has the structural characteristics of chip formation and miniaturization, and can be used for detecting various types of high-throughput proteins at the same time.

The invention belongs to the technical field of biology, and particularly relates to a method for detecting and enriching glycosylated RNA (Ribonucleic Acid) based on in-vitro enzyme chemical labeling of alpha-2, 3, alpha-2, 6 type sialyltransferase. According to the application, sialidase is firstly used for digesting sialic acid at the tail end of a naturally generated glycoRNA sugar chain, and sialidase digestion is not needed for an RNA sample which does not have sialic acid at the tail end of the glycoRNA sugar chain but has potential sialic acid modification sites; sialic acid with a labeled group is transferred to a potential sialic acid modification site at the tail end of a glycoRNA carbohydrate chain by using alpha-2, 3, alpha-2, 6 type sialyltransferase, such as PmST1, PmST3, Ph2, 3siat and Pd26ST, so that glycoRNA with a detectable group can be directly obtained, or the detectable group is further introduced through subsequent reaction, so that subsequent signal detection or enrichment can be carried out; the RNA part can be further sequenced, and the glycosyl part can be further subjected to mass spectrum identification. The application provides a very efficient, simple and convenient detection means for detection and enrichment of sialylated glycoRNA, has the characteristics of high specificity and high sensitivity, and has a wide application prospect.

The invention discloses an unmarked quantum dot electrochemical luminescence sensing electrode for detecting mycotoxin as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing an interdigital electrode pair with two groups of independent detection channels on a glass substrate; self-assembling cysteamine on the surface of the gold working electrode to form an amination interface; sequentially carrying out covalent immobilization on the ZnCdS (at) ZnS quantum dots and different fungaltoxin aptamers through amidation reaction; and finally, sealing by using bovine serum albumin. The prepared sensing electrode has a unique complementary segmented interdigital structure, comb tooth parts of the gold working electrode and the platinum counter electrode are arranged in a staggered and non-contact manner, and annular parts are arranged in a concentric circle manner, so that electric field interference between channels is effectively avoided. The invention further provides application of the electrode, chemical labeling is not needed in the whole process of the method, high-sensitivity, high-specificity and parallel in-situ detection of high-harm mycotoxins such as ochratoxin A and zearalenone is achieved, the integration level is high, and the electrode is suitable for on-site rapid screening of food safety.

This invention relates to the field of biodetection technology, specifically disclosing a multiplex fluorescent staining kit for tissue samples. The kit includes an antibody dissociation buffer, a signal amplification deposition solution, a fluorescent labeling reagent, a tissue-protective imaging medium, and an autofluorescence background inhibitor. This invention utilizes a non-destructive elution technique achieved through the synergistic effect of a low-pH buffer and a directional electric field. It leverages the positively charged nature of antibodies under acidic conditions to drive them away from the tissue and towards the negative electrode, achieving gentle and efficient antibody removal. Simultaneously, it employs a two-step cascade amplification strategy of small molecule deposition and copper-free click chemical labeling, balancing deep penetration with ultra-high sensitivity. This invention can stably support 6-12 cycles of staining, exhibits high elution efficiency, preserves tissue morphology, and can detect low-abundance targets. It is suitable for spatial analysis of the tumor microenvironment, monitoring of transplant immune rejection, and pathological evaluation of neurodegenerative diseases, demonstrating promising clinical application prospects.

The application belongs to the field of chemical analysis, and particularly relates to a method for quantitatively analyzing intact proteins based on mass spectrometry tags. The specific technical scheme comprises the following steps: labeling a protein to be measured; separating the labeled protein by using a size exclusion chromatography-mass spectrometry combined technology; and quantitatively analyzing the protein to be measured by using a mass spectrometry mode of in-source fragmentation combined with multiple reaction monitoring. The application realizes the top-down high-sensitivity absolute quantification of intact proteins on a low-cost QqQ-MS by using a chemical labeling-in-source fragmentation-MRM combined strategy. The method for quantitatively analyzing proteins provided by the application has strong universality and good instrument compatibility.

This invention discloses a chemiluminescent label, a chemiluminescent labeling complex, and their applications, belonging to the field of chemiluminescent immunoassay technology. The chemiluminescent label of this invention is an acridine-pentafluorophenyl ester, prepared by NSP-DMAE-NHS through alkaline hydrolysis, Soxhlet reaction, and substitution reaction. This invention uses this chemiluminescent label for immunoprotein labeling and applies the chemiluminescent labeling complex to a chemiluminescent assay kit. The chemiluminescent label of this invention is not prone to spontaneous hydrolysis and exhibits good stability. In application, it shows low pH requirements for the labeling reaction, achieving good chemical labeling within a fairly wide pH range of 6.0–10.0. The kit prepared by this invention also exhibits higher stability, showing a slow decrease in luminescent activity in accelerated stability studies under high temperature and light irradiation, thus solving the technical problem of insufficient stability encountered by existing acridine esters as chemiluminescent labels.

The present application relates to a kind of high-throughput proteome quantification methods of multi-channel chemical label combined data non-dependence acquisition mode.The method includes:1) prepare 14-plex new chemical label;2) extract the protein of target sample, and enzymatically hydrolyze into polypeptide, and 14-plex tandem mass spectrometry label reagent is used to mark the polypeptide of 14 samples respectively and carries out mixed sample;3) establish the DDA mass spectrum spectrum library of target sample;4) collect the DIA data of polypeptide sample marked with stable 14-plex tandem mass spectrometry label;And 5) using AI algorithm, based on the DIA data collected to identify and quantify target peptide.This application prepares new 14-plex tandem mass spectrometry label, combined with DIA high-throughput proteome quantification method can realize the same peptide segment in different label channel in mass spectrum same window and same scanning being collected, realizes accurate difference quantification of each channel.

The application discloses a chemical labeling system HIM-IP for detecting cell-cell interaction. The system is constructed by modifying tyrosinase and phenol on the surfaces of two cells to be detected respectively, constructing a trapper cell and a prey cell, and then using biotin or single-stranded DNA with a nucleophilic group as a molecular tag for labeling. The system can not only be detected by traditional flow cytometry, but also be compatible with commercial single-cell transcriptome sequencing platforms, realizing the correlation of cell-cell interaction information and transcriptome information at the single-cell level. The system does not need complex genetic engineering modification of cells, the whole modification and labeling process is simple and efficient, and the detection sensitivity of cell-cell interaction is high. By integrating single-cell sequencing technology, the biological mechanism of cell-cell interaction regulation can be further excavated.