Nucleic acid aptamers for detecting extracellular vesicles of human lung cancer cell strain A549 and application of nucleic acid aptamers

A nucleic acid aptamer and lung cancer cell technology, applied in the fields of biomedicine and clinical medicine, can solve problems such as poor treatment effect, complicated pathogenesis, and difficulty in discovery, and achieve the effects of easy modification, simple preparation, and low cost
CN113528531APending Publication Date: 2021-10-22SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2021-10-22

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Abstract

The invention discloses nucleic acid aptamers for detecting extracellular vesicles of a human lung cancer cell strain A549. Nucleotide sequences of the nucleic acid aptamers are shown as SEQ ID NO: 4 (Ap3) and SEQ ID NO: 5 (Ap6). Or the nucleic acid aptamers are a series of derivatives which are obtained through chemical modification, chemical labeling or basic group change on the basis of SEQ ID NO: 4 (Ap3) and SEQ ID NO: 5 (Ap6) sequences. The nucleic acid aptamers disclosed by the invention can be applied to the recognition of extracellular vesicles of the human lung cancer cell strain or the preparation of a kit for detecting the extracellular vesicles of the human lung cancer cell strain or a combination of extracellular vesicle drug delivery and specific recognition of targets. The nucleic acid aptamers can be applied to identification of human lung cancer or preparation of the kit for detecting human lung cancer. The nucleic acid aptamers have very wide application prospects in detection, diagnosis and treatment of human lung cancer.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine and clinical medicine, and specifically relates to a nucleic acid aptamer for detecting extracellular vesicles of human lung cancer cell line A549 and an application thereof. Background technique

[0002] Lung cancer is the leading cause of cancer-related death. Globally, it is divided into two histological types: non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Non-small cell lung cancer is the most common type of lung cancer, accounting for about 80% of all lung cancers. It has the characteristics of high incidence, complex pathogenesis, difficulty in early detection, and poor treatment effect. Moreover, the prognosis of NSCLC patients largely depends on the stage of the disease. For example, the 5-year survival rate drops from 60% in clinical stage I to 1% in clinical stage IV. Early diagnosis of lung cancer is urgently needed. Therefore, the development of efficient,...

Claims

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