The invention discloses a primer construction method for detecting hepatic
echinococcosis through ddPCR, an amplification primer and application of the amplification primer. The primer construction comprises the following steps that
common gene segments of
echinococcus granulosus and echinococcus multilocularis are screened, and a plurality of first primers are designed based on the
common gene segments; a
plasma cfDNA sample of a first
patient group is amplified by adopting the first primers, and the first primers capable of amplifying the
plasma cfDNA sample of the first
patient group are taken as second primers; and a
plasma cfDNA sample of a second
patient group is detected by adopting ddPCR of a
reaction system comprising the second primers, and the second primers with the positive
detection rate greater than a preset value are screened out as a target amplification primer. The amplification primer can amplify
DNA fragments of echinococcus which are released into
peripheral blood and exist in the form of plasma
free DNA, by combining with a ddPCR method, whether
human plasma is infected with
echinococcosis can be diagnosed by detecting the
human plasma, so that the ultra-early diagnosis of
echinococcus granulosus and echinococcus multilocularis is realized, and a detection tool which is sensitive enough is provided for research and development of an
echinococcosis precise therapy.