Preparation method of organoid spheres

A technology of organoids and spheroids, which is applied in the field of preparation of organoid spheroids, can solve the problems that organoids cannot be prepared in high throughput, affect the accuracy and repeatability of prediction results, and the shape and size of organoids are not uniform, reaching the modeling cycle Short, controllable shape and structure, low cost effect
CN110004111AActive Publication Date: 2019-07-12TSINGHUA BERKELEY SHENZHEN INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TSINGHUA BERKELEY SHENZHEN INST
Publication Date
2019-07-12

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Abstract

The invention provides a preparation method of organoid spheres. The preparation method comprises the steps that matrigel containing cells and fluorocarbon oil are introduced into a three-way device to obtain cell spheres, and after culture is performed, the organoid spheres are formed; according to the preparation method of the organoid spheres, the micro-fluid control method is adopted for generating mono-disperse biomaterial organs, high-throughput production is achieved, the sizes, shapes and structures of organoids are controllable and uniform, and compared with a 2D tumor sensitivity predicting model, the organoids are combined with tumor micro-environment factors, and the prediction result is more accurate; compared with a PDX mouse tumor sensitivity predicting model, the modeling period is shorter, and the cost is lower; compared with common gene sequencing, the clinic predicting rate is higher; the preparation method of organoid spheres has the important significance and valuefor clinic application, and the important basis is supplied to detection of related results.
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Description

technical field

[0001] The invention belongs to the field of medical devices and relates to a method for preparing organoid spheroids. Background technique

[0002] Organoids are multicellular clusters constructed by three-dimensional (3D) culture in vitro, which have the ability of self-renewal and self-organization, and maintain the characteristics of the physiological structure and function of their source tissues. Tumor organoids are organoids derived from tumor tissues, which are new and powerful tools for studying tumor biology. Organoid technology is the fastest-growing type of in vitro culture technology in the past decade, and was once hailed as one of the most important scientific advances in 2013. In 2014, Lancaster and Knoblich formally made a systematic scientific definition of organoids, laying a theoretical foundation for the future development of organoid technology.

[0003] The current organoid culture technology is to encapsulate pluripotent embryonic st...

Claims

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