112 results about "Gene expansion" patented technology

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention provides a primer group for identifying coilia ectenes, coilia brachygnathus and hybrids thereof, a detection method, a kit and application, and belongs to the technical field of molecular marker identification. In order to overcome the defects that morphological differences of coilia ectenes, coilia brachygnathus and hybrid adults are not obvious and the like, a primer pair A or a primer pair B is designed for gene amplification, and the coilia ectenes, the coilia brachygnathus and the hybrid adults are identified by comparing sequence differences after amplification. The sequences of amplification products of the coilia ectenes, the coilia brachygnathus and the hybrids have obvious differences, the types of the amplification products can be classified through molecular markers, and the reaction result is directly visualized in the form of a gel electrophoretogram without a sequencing means. The method is simple to operate, intuitive in result, low in requirement on experimental equipment and low in cost, and all work can be completed within several hours without statistics. According to the invention, the hybrid can be identified through a sequencing peak map, and compared with an SNP site identification type, the difference is more obvious, the statistical frequency is not needed, and the accuracy is high.

The invention relates to a group of specific amplification primers for mitochondrial genes of procambarus clarkia and application thereof, and belongs to the technical field of molecular biology, and the mitochondrial genes of the procambarus clarkia comprise cytochrome c oxidase I subunit genes and cytochrome b genes. The specific amplification primer of the CO I gene comprises a CO I gene upstream primer and a CO I gene downstream primer, wherein the base sequences of the CO I gene upstream primer and the CO I gene downstream primer are respectively shown as SEQ NO.1 and SEQ NO.2; the specific amplification primer of the Cytb gene comprises a Cytb gene upstream primer and a Cytb gene downstream primer of which the base sequences are respectively as shown in SEQ NO.3 and SEQ NO.4. The invention further discloses a kit for detecting the Cytb gene. According to the amplification method of the mitochondrial gene of the procambarus clarkia, PCR (Polymerase Chain Reaction) amplification is carried out on a DNA (Deoxyribonucleic Acid) template solution of the procambarus clarkia by adopting amplification primers of a mitochondrial COI gene and a Cytb gene of the procambarus clarkia. The procambarus clarkia mitochondrial gene specific amplification primer has high specificity, a single target gene can be stably amplified, the length of the obtained procambarus clarkia mitochondrial COI gene segment reaches about 1000 bp, and the length of the obtained procambarus clarkia mitochondrial Cytb gene segment reaches about 800 bp.

The invention relates to the technical field of biomedical detection, in particular to a genetic typing primer group, a kit and a genetic typing method for accurately realizing an ultrahigh resolution level of 11 sites of human leukocyte antigen (HLA) based on a nanopore sequencing technology. According to the genetic typing primer group for the HLA ultrahigh resolution level, the primer group is used for HLA gene multiple amplification, and the primer group comprises an HLA-A site primer pair, an HLA-B site primer pair, an HLA-C site primer pair, an HLA-DRB1 site primer pair, an HLA-DRB3 / 4 / 5 site primer pair, an HLA-DQA1 site primer pair, an HLA-DQB1 site primer pair, an HLA-DPA1 site primer pair and an HLA-DPB1 site primer pair. According to the primer group for multiple amplification of 11 genes for HLA typing, disclosed by the invention, the amplification of each gene can be simultaneously carried out on the loci of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 of the HLA genes in a reaction system, so that the integrity, the amplification efficiency and the amplification uniformity of the gene amplification as well as the accuracy of subsequent HLA gene typing are improved.

A space transcriptome in-situ sequencing method for tissue slices relates to the field of gene sequencing, and comprises the following steps: 1) capturing mRNA in situ; 2) carrying out reverse transcription to obtain cDNA; (3) removing tissues and retaining cDNA (complementary deoxyribonucleic acid); 4) carrying out cDNA probe hybridization; 5) performing in-situ amplification; 6) decoding and sequencing the fluorescent probe; and 7) cleaning the probe, and sequencing for multiple rounds. According to the invention, RNA is captured in situ and is reversely transcribed into cDNA, nucleic acid imprinting transfer is realized, background interference is removed, the problem of RNA degradation is solved, and multiple rounds of hybridization are allowed to increase the detection flux. The nucleic acid sequence is covalently bound with a slide to prevent a signal point from shifting and falling off. A DNA coding padlock probe is used for identifying genes, amplifying and amplifying signals, and gene expression is revealed through sequencing and decoding. After the probe is cleaned, the probe is replaced for hybridization amplification, so that the flux is improved, and space crowding and fluorescence signal crowding are reduced.

The present invention discloses an application of an insect virus FHV RNA1 replicon in exogenous gene amplification, wherein the application amplifies the exogenous gene at the mRNA level by utilizing the autonomous replication ability of the insect virus FHV RNA1 replicon; the present invention discloses a plasmid system based on the insect virus FHV RNA1 replicon and a method for constructing the plasmid system; the present invention also discloses a method and application of the plasmid system for expressing proteins in cells, wherein the plasmid system is used to obtain a cloning vector after double enzyme digestion, and after homologous recombination with an exogenous gene, the animal or plant protein is expressed in the cell.

The invention discloses an HLA (human leukocyte antigen) gene amplification primer based on a nanopore sequencing platform and a typing method, and relates to the technical field of gene engineering, the amplification primer comprises an HLA-A gene amplification primer pair: a forward primer HLA-A-F5; a reverse primer HLA-A-R5; an HLA-B gene amplification primer pair: a forward primer HLA-B-F3; a reverse primer HLA-B-R3; the HLA-DQB1 gene amplification primer pair comprises a forward primer HLA-DQB1-F7, a reverse primer HLA-DQB1-F7 and a reverse primer HLA-DQB1-F7; a reverse primer of HLA-DQB1-R7; the HLA-DRB1 gene amplification primer pair comprises a forward primer HLA-DRB1-F1, a reverse primer HLA-DRB1-F2, a reverse primer HLA-DRB1 A reverse primer (HLA-DRB1-R1); the HLA-DRB3 gene amplification primer pair comprises a forward primer HLA-DRB3-F6, a reverse primer HLA-DRB3-F6, a reverse primer A reverse primer (HLA-DRB3-R8); by designing the specific amplification primer group and combining with the nanopore sequencing length reading technology, the problems of insufficient resolution and the like of the existing typing technology are solved, the typing accuracy and resolution are improved, and the performance is superior to that of the traditional technology; meanwhile, the process is optimized, the limitation of long detection period and the like is broken through, the whole process is rapidly completed, the technical threshold and detection expenditure are reduced, high efficiency, convenience and economy are considered, and support is provided for clinical popularization and scientific research application.

The present invention provides a primer pair comprising a combination of sequence numbers 3 and 7, a combination of sequence numbers 2 and 9, a combination of sequence numbers 1 and 8, a combination of sequence numbers 1 and 9, a combination of sequence numbers 4 and 11, a combination of sequence numbers 5 and 12, a combination of sequence numbers 6 and 10, or a combination of sequence numbers 6 and 12 as a primer for detecting a methicillin resistance gene for realizing highly sensitive methicillin resistance gene detection.

The present invention belongs to the technical field of sequencing in gene therapy. Provided is a detection method for a rare-mutation BRAF gene. The sequencing method comprises: during an amplification process of the BRAF gene of a target sample fragment, adding a blocking primer matched with a wild-type template in the target sample fragment, so as to bind the blocking primer to a denatured wild-type template; and performing sequencing to obtain a sequencing result corresponding to the target sample fragment. During the process of making a sequencing template, the present invention uses the blocking primer to inhibit the wild-type template of the BRAF gene during the amplification process, so as to relatively increase the proportion of a mutant product, thereby achieving the purpose of accurately detecting samples with a lower mutation rate. Therefore, the present method is of an important significance for detecting rare mutations by using a Sanger sequencing method.

The invention belongs to the technical field of microbiological detection, and particularly relates to a Klebsiella pneumoniae MLST typing primer group and method based on a hospital localized microbiological mNGS platform. The primer group comprises primer pairs for amplifying an rpoB gene, an infB gene, a pgi gene, a phoE gene, a gapA gene, an mdh gene and a tonB gene, and corresponding nucleotide sequences are as shown in SEQ ID NO: 1-14. According to the technical characteristics of short sequencing read length (50 bp) and single-ended sequencing of a next-generation sequencing platform in a hospital, the primer group for Klebsiella pneumoniae MLST housekeeping gene amplification is redesigned, and amplicons are adaptive to the sequencing working principle of the existing next-generation sequencing platform in the hospital by optimizing the length and position of the amplicons. By utilizing the primer group, the kit or the method, the genetic relationship between hospital infection pathogenic bacteria can be quickly and accurately judged (within 24 hours), the timeliness and scientificity of infection prevention and control are remarkably improved, and the primer group, the kit or the method have a wide clinical application prospect.

The invention relates to the field of molecular biology diagnosis, in particular to a detection kit for SMN1 and SMN2 gene sequence variation, and gene variation types mainly comprise SMN1 and SMN2 gene point variation, insertion and deletion variation in a gene range and the like. The kit provided by the invention can realize detection and diagnosis of SMN1 and SMN2 point variation, small insertion and deletion, sequence variation of SMN1-SMN2 partial transformation types, structural recombination in genes and the like.

The present invention discloses a gene detection device, which relates to the field of gene detection and includes a safety cabinet, an air flow control system, a first processing module for processing amplification reagents and PCR plates, a second processing module for processing reagents, pipette tips and gene samples, a sample addition module for adding amplification reagents into the micro-wells of the PCR plate, a purification processing module for processing gene samples, an amplification and detection module for gene amplification and detection, a heat sealer for sealing the PCR plate, a logistics transportation module A, a logistics transportation module B and a logistics transportation module C; through the gene detection device of the present invention, automated, pollution-free and high-efficiency gene detection is achieved. At the same time, during the detection process, only a small part involves manual operation, and the manual operation intensity is low.

The present invention relates to the field of marine biology technology, and specifically to a method for analyzing food residues in the gastrointestinal tract of Antarctic krill using molecular sequencing technology. The method comprises the following steps: dissecting Antarctic krill in a low-temperature environment and obtaining total DNA and total RNA using a nucleic acid extraction kit, and amplifying target genes in combination with specific primer design; subsequently, barcode labeling and high-throughput sequencing library construction are performed, paired-end sequencing is performed using an Illumina NovaSeq platform, and data quality control and redundancy removal are completed to obtain high-quality sequence data, accurate species identification is performed based on multi-level sequence alignment and a lowest common ancestor algorithm, phylogenetic tree and network analysis methods are used to analyze the diversity and trophic level distribution of Antarctic krill food residues, and food sources are further traced through environmental DNA comparison. The present invention can significantly improve the accuracy and scope of species identification and is of great significance for understanding the energy flow and trophic level structure of the Antarctic ecosystem.

The invention discloses a high-throughput single cell transcriptome sequencing and library building method based on microfluidics. The method can be expanded to be used for information acquisition of various transcripts. A single cell and RNA, a single-cell molecular tag, a reverse transcription reagent and a PCR reagent carried by the single cell are fixed in a partition through a one-button automatic instrument, labeling of the single cell in the single partition is achieved through the single-cell molecular tag in the partition, reverse transcription of the RNA in the single partition is achieved through the reverse transcription reagent, and cDNA is obtained through PCR. Signal amplification of cDNA of a single cell in a single partition is realized through a PCR reagent. After a cDNA signal in a single partition is amplified, the partitions are broken, cDNA products carrying single-cell molecular tags of the multiple partitions are combined, the cDNA products are subjected to fragmentation and then introduced into a sequencing linker, and library construction is completed. According to the method provided by the invention, the loss of cDNA products is reduced while experimental operation steps are reduced, so that the quantity of gene amplification products is increased by 40% or above.

The present application relates to a kind of triplex-forming oligonucleotides (TFOs) sequence and its pharmaceutical application, especially to a kind of triplex-forming oligonucleotides sequence and its pharmaceutical application for inhibiting MDM2 and MDM4 gene amplification.The triplex-forming oligonucleotides sequence provided by the present application can form triplex structure locally in the amplified MDM2 / MDM4 gene of tumor cell, cause its replication stress, finally lead to the apoptosis of tumor cell.The triplex-forming oligonucleotides sequence provided by the present application and the medicine prepared using the sequence are aimed at the MDM2 / MDM4 amplification gene sequence of tumor rather than protein, have good drug resistance, and have no effect on normal cell without MDM2 / MDM4 gene amplification, have excellent safety, thus have good therapeutic effect on the tumor with MDM2 / MDM4 gene amplification.

The application discloses application of a peach fruit hexokinase PpHXK3 gene in preparation of a plant cold-resistant product, and is characterized in that the nucleotide sequence is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2; a cloning method of PpHXK3 comprises the following steps: extracting total RNA of peach fruit and reversely transcribing the total RNA into cDNA as a template, and designing upper and lower amplification primers according to a gene sequence of PpHXK3; finally, a PpHXK3 gene amplification product is obtained through PCR amplification; the application of PpHXK3 in preparation of polygalacturonase PpPG21 inhibitors and / or polygalacturonase PpPG23 inhibitors, and the application of PpHXK3 in preparation of peach fruit cold-resistant products and heterologous plant cold-resistant products are also provided; and the advantages are that the decomposition of pectin is reduced, the integrity of a cell wall structure and the hardness of a fruit are maintained, the fruit has higher cold resistance, and cold damage in a storage and transportation process is reduced.

The invention discloses a preparation method and application of a quality control product or a reference product for HER2 gene amplification detection, and relates to the technical field of molecular biology. Compared with a quality control product or a reference product of a clinical sample or a cell line, the synthesized plasmid is adopted for preparing the quality control product and the reference product, biological materials of the synthesized plasmid are easier to obtain, wide in source and easier to prepare on a large scale at low cost, and the prepared quality control product and the reference product have good accuracy and repeatability, small difference between preparation batches and high application value. By designing plasmids, quality control products with different HER2 amplification levels can be prepared according to the repetition times of HER2 gene segments, and the preparation method is simple and convenient, can be used for batch production, is easy to store, can be stored for a long time under the condition of-20 DEG C, and can be used for evaluating the accuracy and repeatability performance of an HER2 gene detection method.

The invention relates to the technical field of gene detection, in particular to a rapid shrimp iridovirus detection primer group based on an RPA-CRISPR / Cas12a system, application and a kit. The primer group comprises an iridovirus constant-temperature gene amplification primer and a specific recognition amplification product target sequence probe; wherein the iridovirus constant-temperature gene amplification primer, the specific recognition amplification product target sequence CRISPR probe and the ssDNA reporter probe are recorded in the specification. An isothermal amplification primer and CrRNA are designed based on the shrimp red color virus gene, the target gene is amplified through the isothermal amplification technology, Cas12a is guided by the CrRNA to accurately recognize and activate trans-cleavage, a test strip report probe is synchronously split, and the result is interpreted by naked eyes within 30 min by virtue of the visual advantage of an immune test strip. The whole process does not need large instruments, the operation is simplified, the cost is low, and a visual and efficient solution is provided for on-site rapid diagnosis.

The invention discloses a method for identifying aquilaria agallocha and aquilaria sinensis by using a DLAT gene. Aquilaria agallocha and aquilaria sinensis are identified on the basis that the DLAT genotype of the Aquilaria agallocha is TT and the DLAT genotype of the aquilaria sinensis is CC. A No.1879 basic group of a DLAT gene coding region of agilawood aquilaria sinensis and aquilaria sinensis is taken as a target site, a PCR and Sanger sequencing method is adopted, a PCR amplification product of the DLAT gene is subjected to Sanger sequencing, if the genotype of the DLAT is TT, the product is agilawood aquilaria sinensis, and if the genotype of the DLAT is CC, the product is aquilaria sinensis. According to the method for identifying the agilawood agilawood and the aquilaria sinensis through the DLAT gene, high-throughput sequencing is carried out on the agilawood agilawood and the aquilaria sinensis, genotype detection analysis is carried out in a whole genome range, and it is found that the DLAT gene has significant difference between the agilawood agilawood and the aquilaria sinensis, and the gene function is clear. The method is used for identifying the germplasm of the agilawood agilawood and the aquilaria sinensis by utilizing the genotype difference of the agilawood agilawood and the aquilaria sinensis and adopting PCR amplification and Sanger sequencing methods

The invention discloses an SNP (Single Nucleotide Polymorphism) site, a DNA (Deoxyribose Nucleic Acid) fragment, a molecular marker primer and a method for identifying the color of cerasus humilis flesh, the SNP site is located at the 422nd basic group site of a cerasus humilis DFR gene, the polymorphism is T / G, the SNP site of red flesh is T, and the SNP site of yellow flesh is G. The nucleotide sequence of the DNA fragment is as shown in SEQ ID NO.1 or SEQ ID NO.2, and the molecular marker primer comprises a forward primer as shown in SEQ ID NO.3 and a reverse primer as shown in SEQ ID NO.4. The molecular marker primer has the advantages that the nucleotide sequence of the DNA fragment is as shown in SEQ ID NO.1 or SEQ ID NO.2; the method comprises the following steps: (1) collecting a to-be-detected cerasus humilis plant tissue sample and extracting total RNA (Ribonucleic Acid); (2) carrying out reverse transcription on the total RNA to obtain cDNA (complementary deoxyribonucleic acid); step (3), amplifying the cerasus humilis DFR gene by using the molecular marker primer by taking the cDNA as a template; (4) purifying and sequencing an amplification product; and (5) judging whether the pulp of the to-be-detected cerasus humilis sample is red or yellow according to a sequencing result. The technical problems that a traditional cerasus humilis pulp color identification method is long in period and not beneficial to popularization can be solved.

1. The name of the design product: Gradient gene amplification instrument (gradient PCR instrument). 2. The use of the design product: for gene amplification reaction. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

Provided is a device for amplifying and detecting a gene. The device for amplifying and detecting the gene includes a gene amplification chip comprising channels through which a sample flows and transparent heaters provided on the gene amplification chip. The channels include a first channel, a second channel, and a third channel, and the first to third channels have a triangular loop structure, and the transparent heaters include a first transparent heater, a second transparent heater, and a third transparent heater, which are respectively provided on the first to third channels.

The invention discloses application of a peach fruit hexokinase PpHXK3 gene in preparation of a plant cold-resistant product. The peach fruit hexokinase PpHXK3 gene is characterized in that a nucleotide sequence is shown as SEQ ID NO.1, and an amino acid sequence is shown as SEQ ID NO.2; the cloning method of the PpHXK3 comprises the following steps: extracting total RNA (Ribonucleic Acid) of peach fruits, reversely transcribing the total RNA into cDNA (Complementary Deoxyribonucleic Acid) as a template, designing upstream and downstream amplification primers according to a PpHXK3 gene sequence, and finally, carrying out PCR (Polymerase Chain Reaction) amplification to obtain a PpHXK3 gene amplification product. The invention further provides application of the PpHXK3 in preparation of a polygalacturonase PpPG21 inhibitor and / or a polygalacturonase PpPG23 inhibitor and application of the PpHXK3 in preparation of peach fruit cold-resistant products and heterologous plant cold-resistant products, and the PpHXK3 has the advantages that decomposition of pectin is reduced, integrity of a cell wall structure and hardness of fruits are maintained, so that the fruits have high cold resistance, and the yield of the fruits is increased. And the cold damage in the storage and transportation process is reduced.

The invention relates to the technical field of medical detection, in particular to magnetic bead mediated gene amplification integrated equipment which comprises an instrument body and a detection assembly, and the detection assembly is mounted on the instrument body. The detection assembly comprises a detection tube and a test tube. The detection tube comprises a first chamber and a second chamber which are sequentially arranged in the first direction, the first chamber comprises a first side wall and a second side wall, and a flattening piece is arranged on the first side wall. According to the magnetic bead mediated gene amplification integrated equipment disclosed by the invention, a whole machine is adopted to enable a sample to be subjected to the steps of splitting decomposition, cleaning and amplification in sequence, air is blown into the first chamber or sucked from the first chamber through the second chamber, and the driving piece can drive the flattening piece to move close to or away from one side of the second side wall in the second direction; the reaction efficiency and the reaction effect of the magnetic bead mediated gene amplification integrated equipment are improved on the whole.

The present invention relates to a microfluidic chip, a cartridge, and a genetic analysis device including same and, particularly, to a device that includes compact analysis tools for a gene amplification reaction and capable of accommodating solutions therefor, ensures rapid analysis results on-site, and can automatically perform genetic analysis processes.

The invention relates to the technical field of molecular biology, in particular to a primer group, a kit and a method for detecting GII type norovirus. The invention discloses a primer group for detecting a GII type norovirus. The primer group can be used for LAMP (Loop-Mediated Isothermal Amplification) to quickly amplify the sequence of the GII type norovirus. The invention further discloses a method for rapidly detecting the GII type norovirus, according to the method, a certain amount of dUTP and UDG are added into an LAMP amplification system, the probability of false positive can be reduced, meanwhile, gene amplification is conducted through LAMP, the method has the advantages of being rapid and convenient to use, an amplified product is tested through LFD, and a result can be visually displayed on LFD. The method has the advantages of rapidness, high efficiency and certain anti-pollution capability.

The utility model discloses a gene amplification instrument with a test tube easy to take out, which comprises a machine body provided with a test tank and hinged with a tank cover; the heating module is provided with a plurality of test tube insertion holes, and the plurality of test tube insertion holes are arranged in a multi-row and multi-column matrix; the plurality of up-and-down lifting structures are arranged corresponding to the plurality of rows of test tube insertion holes, and the up-and-down lifting structures can move up and down relative to the machine body; and the lifting driving structure drives the multiple up-down lifting structures to move up and down, so that the multiple up-down lifting structures are arranged in a stepped fall mode. When the groove cover is turned upwards, the lifting driving structure drives the multiple up-down lifting structures to be arranged in a stepped fall mode. The vertical lifting structure can drive the test tubes to ascend in the ascending process, so that the multiple rows of test tubes are arranged in a stepped fall mode, and by means of the arrangement, under the condition that the multiple test tubes are tightly arranged, workers can easily take out the test tubes, and the test tubes can be taken out more conveniently.

This automated gene testing device enables accurate and rapid determination of gene amplification reactions within a reaction vessel through precise dispensing operations controlled by the rotation of a microfluidic chip and precise heating operations controlled by the temperature of the microfluidic chip. It allows for simple and rapid genetic testing of multiple viruses and other pathogens at the genetic level, even in on-site settings, without requiring special expertise or skills. [Solution] A gene amplification reaction is performed using a disc-shaped test chip equipped with a microfluidic centrifugal dispensing function, comprising a liquid delivery unit, a heating unit, an imaging unit, a display unit, and a control unit. The test chip comprises an injection reservoir set with a liquid sample and multiple reaction chambers set with primers. The liquid delivery unit comprises a drive source and a rotating shaft, and the heating unit comprises a heater and a lifting device. The imaging unit comprises a light source and a camera, and the display unit comprises a determination device and a display device.

1. The name of the design product: Gene Amplifier (automated). 2. The use of the design product: for amplification of nucleic acid fragments. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.