7 results about "Coi gene" patented technology

The application relates to a primer probe composition, a kit and a detection method for specifically detecting Blattella germanica. The specific primer and TaqMan probe are designed and screened based on the specific sequence of the mitochondrial COI gene of Blattella germanica. The detection method has extremely high specificity, has no cross reaction with close species such as Periplaneta americana and Blattella germanica, has high sensitivity, the minimum detection limit is 1 fg / muL, and has good repeatability, with the variation coefficients of groups and between groups being less than 3%.

The invention belongs to the field of biotechnology detection, and discloses a fluorescent quantitative PCR (polymerase chain reaction) primer probe composition for pseudorasbora parva and application. A specific upstream primer, a specific downstream primer and a TaqMan probe are designed according to a COI gene sequence in a mitochondrial DNA sequence of the pseudorasbora parva, the primer is obtained through comparison and screening, the primer has the characteristic of high specificity, the fluorescent quantitative PCR method established based on the primer can efficiently, accurately and specifically identify the pseudorasbora parva through genome DNA, the influence of subjective factors is eliminated, and the primer is high in sensitivity and high in specificity. The repeatability is good; and the method can be used for quantitatively analyzing the eDNA of the pseudorasbora parva, so that the pseudorasbora parva can be efficiently, accurately and specifically identified without damaging the pseudorasbora parva, and technical support can be provided for monitoring, protecting and managing population resources of the pseudorasbora parva which are specifically distributed in narrow areas in China.

The invention relates to the technical field of oyster cross breeding, in particular to a COI genetic distance-based crassostrea calthrop hybridization prejudgment and combined configuration method. The method comprises the following steps: collecting COI gene sequences of calthrop oysters and four cultured oyster samples, constructing an allelic point sliding window, and calculating genetic differences by adopting a Kimura-2P distance, so as to obtain a refined COI genetic distance index; establishing a COI heredity-hybridization advantage regression model by combining historical hybridization survival data, heredity variation data and disturbance factors, introducing a multi-dimensional threshold structure based on genetic distance, and performing regression pre-judgment of threshold logic regulation and control on the hybridization advantage; on the basis, a COI genetic distance matrix is constructed, intraspecific fertility analysis and character complementation inference are coupled, and optimization and combined configuration of cross parents are achieved. Quantitative prediction of the hybridization advantage can be achieved before hybridization, the breeding period is shortened, the hybridization test cost is reduced, and the accuracy of breeding hybridization auxiliary decision making is remarkably improved.

Disclosed is a pair of specific primers for identifying Asian gypsy moth and a method of detection thereby. In the present disclosure, based on the SS-COI technology, primers for distinguishing other species of Lymantria are designed according to the specific region of the mitochondrial COI gene of Asian gypsy moth. The pair of primers has accurate identification effects on different geographical populations, different insect states and different instars of Asian gypsy moth; in addition, the whole detection process only takes 2-3 hours, which has the advantages of rapidity and accuracy.

The invention discloses a CPA primer group and a kit for detecting seven chicken eimeria coccidiosis and application of the CPA primer group and the kit, and belongs to the technical field of biological detection. Specific primers are designed for conservative COI genes of chicken eimeria, an optimal reaction system and 61 DEG C constant-temperature amplification conditions are optimized and determined, and a CPA detection method for detecting chicken eimeria is established. The method is extremely high in sensitivity, the lower limit of detection on a plasmid standard substance reaches 102 copies / L, extremely low pathogen load equivalent to 0.5 oocyst can be stably detected in practical application, the clinical sample verification coincidence rate reaches 100%, and a powerful technical support is provided for early warning, field monitoring and precise prevention and control of chicken coccidiosis.

This invention discloses a dual-gene specific primer set for rapid identification of Chiroptera species and its application method. The primer set includes primer pair COI-F / COI-R for amplifying the mitochondrial COI gene and primer pair Cytb-F / Cytb-R for amplifying the Cytb gene, with nucleotide sequences shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, and SEQ ID NO.4, respectively. Experiments have demonstrated that this primer set exhibits good interspecific specificity and intraspecific conservation, effectively distinguishing Chiroptera from other closely related mammals and accurately identifying cryptic species and juvenile samples that are difficult to distinguish morphologically. This invention employs dual-gene markers for mutual verification, significantly improving the accuracy and reliability of identification, providing a powerful molecular biology tool for rapid and accurate identification of Chiroptera species, and has significant application value in fields such as biodiversity surveys, disease tracing, and ecological conservation.

The invention belongs to the technical field of molecular biology, and particularly relates to a multiplex PCR primer combination for identifying species of pangolin and application of the multiplex PCR primer combination. Three pairs of specific primers are designed aiming at species specific conserved regions in three kinds of pangolin mitochondrial genes and respectively target COI genes of Chinese pangolin, pangolin males and pangolin south African. By utilizing the primer group, synchronous identification of three pangolin species can be realized in the same PCR reaction system, and visual interpretation is performed through agarose gel electrophoresis according to the size of an amplified fragment. The detection method disclosed by the invention has the advantages of rapidness, accuracy, strong specificity, simplicity and convenience in operation and the like, is suitable for species identification of squama manis scales, tissues, blood and products thereof, and provides an efficient and reliable technical means for illegal trade traceability and species protection monitoring and identification of the squama manis.