SS-COI (Sirex noctilio F. specific cytochrome oxidase I) primer pair and quick molecular detection method

Abstract

The invention provides an SS-COI (Sirex noctilio F. specific cytochrome oxidase I) primer pair and a quick molecular detection method. By taking Sirex noctilio F. as a target and other four horntails (Sirex nitidus, Sirex nitobei, Tremex fuscicornis and Tremex apicalis) as references, species specific primers based on mitochondrial COI gene sequences are designed and a quick molecular detection system of the Sirex noctilio F. is constructed and optimized, wherein the Sirex noctilio F. and the other four horntails occur in the same region in China and belong to sibling species.

Description

technical field [0001] The invention relates to the field of molecular detection, in particular to a pair of pine wasp-specific SS-CO I primers and a rapid molecular detection method. Background technique [0002] With the acceleration of global economic integration, the situation of alien species invasion in forestry is becoming increasingly severe. Some major forestry invasive species have caused great losses to my country's forest resources, species diversity and ecological security. [0003] The pine wasp (Sirex noctilio F.) is native to Eurasia and North Africa, and mainly harms pine trees. Listed as a "very high risk" forestry invasive species in the United States. In the last century, the insect successively invaded New Zealand and Australia in Australia, Uruguay, Argentina, Brazil and Chile in South America, and South Africa; at the beginning of this century, it was introduced into Canada and the United States in North America; in severely invaded areas, it can cau...

AI Technical Summary

Problems solved by technology

In order to distinguish the pine wasp from the closely related tree wasp insects, extensive morphological identification work is required, most of which are time-consuming, labor-intensive and require

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