The invention relates to a species-specific PCR method for rapidly identifying ten sea cucumber species, and belongs to the molecular biology field. The method comprises the following steps: respectively designing species-specific primers of the ten species of sea cucumber through sequence alignment by treating mitochondrial 16SrRNA gene of the ten species of sea cucumber as target gene, extracting sea cucumber mitochondrial gene, carrying out specific PCR amplification detection, determining the sea cucumber species comprising Actinopygamauritiana, Stichopusnaso, Cucumariafrondosa, Holothuriaedulis, Holothuriaatra, Holothuriaflavomaculata, Holothuriamammata, Holothuriabacilla, Holothurianotabilis and Holothuriamoebii according to the existence or inexistence of a PCR amplification band, carrying out specific PCR amplification to respectively obtain 274bp, 276bp, 264bp, 275bp, 270bp, 273bp, 274bp, 276bp, 276bp and 270bp specific fragments.