Identification method for bolting character of radish based on FLC gene

An identification method, radish technology, applied in biochemical equipment and methods, microbial measurement/inspection, instruments, etc., can solve problems such as unstable results, long cycle, and restrictions on the breeding process of fine varieties resistant to bolting in spring, and achieve results It is stable and reliable, shortens the identification time of bolting characteristics, and has good application prospects

Inactive Publication Date: 2009-12-09
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] The purpose of the present invention is to study and provide a novel, fast and accurate identification method for bolting characteristics of radish germplasm materials in view of the problem that the identific

Method used

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  • Identification method for bolting character of radish based on FLC gene
  • Identification method for bolting character of radish based on FLC gene
  • Identification method for bolting character of radish based on FLC gene

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Experimental program
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Embodiment Construction

[0029] 1. Cultivation of radish seedlings

[0030] The seeds of radish materials to be tested are soaked in 25°C water for 10 minutes to make the seeds physically absorb water. Take a clean petri dish, spread a layer of wet filter paper on the bottom, spread the seeds evenly, cover the dish lid, put it in a constant temperature box at 25°C to accelerate germination, take it out after 24 hours and sow it in a plug tray, and put it in a greenhouse or a plastic greenhouse (day / night temperature is 25 / 15°C, natural light) growth.

[0031] 2. Extraction of Radish Genomic DNA

[0032] (1) Take about 0.2g young leaves of radish in a mortar. Thoroughly grind into powder with liquid nitrogen, add (0.4mL / time) CTAB lysis buffer twice, transfer to 2.0mL, shake gently, and place in a water bath at 65°C for 30 minutes.

[0033] (2) Take out the centrifuge tube, and after the solution in the tube drops to room temperature, centrifuge at 11200 rpm for 8 minutes, and transfer the supernata...

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Abstract

The invention relates to an identification method for bolting character of radish based on FLC gene, belonging to the biotechnology field. In the invention, DNA differences of gene FLC sequence closely related to bolting and blossoming in early and late bolting character radish varieties are taken as basis to design two pairs of species-specific primers NauFLC-F1/NauFLC-R1 and NauFLC-F2/NauFLC-R2, then PCR technology is used to carry out FLC gene specific amplification on material genomic DNA, and the tested materials are judged to be either early bolting type or late bolting type according to the electrophoresis results rapidly and correctly. The method can be effectively used for identifying the bolting character of radish germplasm materials, and can greatly quicken the breeding process of the good strains of the spring radish bolting resistance rapidly, correctly and efficiently.

Description

technical field [0001] The invention relates to a method for rapidly and accurately identifying the bolting characteristics of plants according to the differences in FLC gene sequences among radish varieties with different bolting characteristics. Belongs to the field of biotechnology. Background technique [0002] Radish (Raphanus sativus L.) is an important worldwide vegetable originating in my country, with chromosome 2n=2x=18 and a genome size of about 526 Mbp (Wang Longzhi and He Qiwei, 2005). Our country has a long history of radish cultivation, and its area ranks second among all vegetable crops, occupying an important position in the production and supply of vegetables in my country (Chinese Agricultural Statistics, China Agricultural Press, 2007). Radish is not only an important vegetable crop for people, but also has a good therapeutic and health care effect. In recent years, domestic and foreign studies have found that radish has anti-cancer, anti-virus, anti-bac...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/447
Inventor 柳李旺黄丹琼龚义勤姜立娜翟璐璐
Owner NANJING AGRICULTURAL UNIVERSITY
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