The invention discloses a six-flavor hematinic capsule, its quality control method and an application thereof. The six-flavor hematinic capsule is prepared by adding auxiliary materials into raw materials consisting of Chinese angelica, Ligusticum wallichii, Radix Astragali, prepared rehmannia root, lithospermum and white peony root. According to the quality control method of the six-flavor hematinic preparation, whether the six-flavor hematinic capsule contains white peony root, Chinese angelica, Radix Astragali, prepared rehmannia root, lithospermum, Ligusticum wallichii and components is identified by thin layer chromatography; by high-performance liquid chromatography, in vitro dissolution behavior of the six-flavor hematinic capsule is determined, and effective component groups are identified by fingerprint; verbascoside and calycosin glucoside are used as reference substances to simultaneously determine contents in the six-flavor hematinic capsule; and the content of a volatile component ligustilide in the six-flavor hematinic capsule is determined by a gas chromatographic method. The method provided by the invention is simple to operate, is accurate and advanced, has good linear relation, reappearance, precision, stability and recovery rate, can be adopted to effectively control product quality and guarantee curative effect of the product. The invention also provides an application in the preparation of a medicine for treating eye damage and blurred vision.