Method for determining multiple kinds of animal origin meat based on liquid chromatographic-tandem mass spectrometric technology

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of determination of various animal-derived meats based on liquid chromatography and tandem mass spectrometry, can solve problems such as false positives and protein denaturation, achieve easy operation, simplify the processing process, and ensure accuracy sexual effect

Active Publication Date: 2016-05-18
CHINA MEAT RES CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ELISA technology is easily restricted by antibody preparation, protein denaturation during processing, and complex matrix leading to false positives; PCR technology is easily affected by DNA degradation, interference from complex matrix, and sample extraction and amplification methods

Method used

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  • Method for determining multiple kinds of animal origin meat based on liquid chromatographic-tandem mass spectrometric technology
  • Method for determining multiple kinds of animal origin meat based on liquid chromatographic-tandem mass spectrometric technology
  • Method for determining multiple kinds of animal origin meat based on liquid chromatographic-tandem mass spectrometric technology

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Embodiment 1

[0036] Example 1 Method for Determination of Various Animal-Derived Meats Based on Liquid Chromatography Tandem Mass Spectrometry

[0037] Include the following steps:

[0038] 1. Sample pretreatment:

[0039] Standard meat sample: weigh 2g of duck meat and 2g of mutton; mixed meat sample: weigh 0.1g of duck meat and 1.9g of mutton. Follow the steps below for pretreatment.

[0040] Total protein extraction: add extract (0.05mol / L Tris-HCl, 7mol / L urea, 2mol / L thiourea, pH8.0), homogenize in an ice-water bath for 1min, then wash the knife head with 5mL extract and combine, 12000r / min Centrifuge for 20min.

[0041] Reduction and alkylation: Aspirate 100 μL of supernatant, add 10 μL of dithiothreitol solution (50 mmol / L), and shake at 56°C for 1 hour. Leave it at room temperature, add 20 μL of iodoacetamide solution (100 mmol / L), and react in the dark for 30 min. Add 15 μL of dithiothreitol solution (50 mmol / L) and react in the dark for 15 min.

[0042] Digestion: Add 750 μ...

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Abstract

The invention provides a method for determining multiple kinds of animal origin meat based on the liquid chromatographic-tandem mass spectrometric technology. The method includes the steps of pretreating samples, screening multiple standard meat sample feature polypeptides, and detecting feature polypeptides in meat samples to be detected through the liquid chromatographic-tandem mass spectrometric technology and the like. Components of the multiple kinds of animal origin meat can be identified by adopting the method, the scanning function of liquid chromatography-tandem mass spectrometry is brought into full play in the technology, the selected ion monitoring mode is monitored to collect signals in real time, second-level daughter ion intensity triggering is used, corresponding parent ion second-level fragmentation full-scanning spectrograms are collected to obtain a more exact qualitative information function, interference of a complex matrix is avoided, and the false positive possibility is reduced. In addition, the method is simple in pretreatment and facilitates operation on samples in batches.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for measuring various animal-derived meats based on liquid chromatography tandem mass spectrometry technology. Background technique [0002] In recent years, the adulteration of meat products has become a food safety issue of global concern. Meat adulteration incidents have been exposed many times. In order to seek illegitimate benefits, some unscrupulous traders use inferior meat as good ones. Unscrupulous operators use relatively cheap duck, chicken, pork and other low-priced meat raw materials to replace high-priced meat such as beef and mutton. Sale. This kind of adulteration is a commercial fraud, which disrupts the market order. At the same time, it involves issues such as ethnic dietary taboos, which seriously violates the legitimate rights and interests of consumers. Therefore, it is of great practical significance to establish an accurate and efficient d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 李莹莹张颖颖赵文涛马燕红王守伟郭文萍任南李志刚李慧晨段姗姗
Owner CHINA MEAT RES CENT
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