This invention proposes an organic field-effect
transistor (OFET)
biosensor based on interface topology
engineering, its fabrication method, and its application, belonging to the field of
biosensor technology. The OFET
biosensor comprises a substrate, electrodes, an
organic semiconductor layer, an interface topology modification layer, and a biorecognition probe. Through a kinetically controlled
vacuum deposition process, an interface topology modification layer with a nanoscale uniform rough morphology is constructed on the
semiconductor surface. This topology induces double-layer overlap at the
solid-
liquid interface, significantly reducing interface
capacitance and physically stretching the effective
Debye length, thereby overcoming the
Debye shielding effect in the high
ionic strength environment of physiological fluids. Simultaneously, the
aldehyde groups on the surface of the modification layer achieve covalent fixation of the probe without connecting arms and provide encapsulation protection. This sensor achieves ultrasensitive detection of
allergen-specific IgE and exhibits excellent stability and
signal-to-
noise ratio in undiluted human serum, providing a universal physical
sensitization strategy for the point-of-care testing of complex clinical samples.