37 results about "Hydrophilic Interactions" patented technology

The present invention relates to a method of forming shape-retentive aggregates of gel particles in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a suspension of gel particles in a polar liquid at a selected concentration, wherein the gel particles have an absolute zeta potential, into a medium in which the absolute zeta potential of the gel particles is decreased, resulting in the gel particles coalescing into the claimed shape-retentive aggregate. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel particles.

The present invention relates to mixed-modal anion-exchange materials composed of a support on which a ligand is immobilized. The ligand combines at least one basic domain based on cyclic monobasic derivatives with two or more rings as anion-exchange domain and at least one non-ionic binding domain. The basic domain is ionized under the conditions of use and may contain secondary, tertiary, or quaternary nitrogen forming a weakly (WAX) or strongly (SAX) basic anionic exchange domains. The non-ionic binding domain allows adjustment of the overall hydrophobicity/hydrophilicity of the material and represents a second binding site for the solute to be separated. Binding to this second binding site is based on reversed phase (RP), hydrophobic interaction (HIC) or hydrophilic interaction (HILIC). Linker sites, which can be represented by a chemical bond or by hydrophobic moieties like alkyl(ene) chains or hydrophilic moieties like amide structures connect the support to the binding domains and the binding domains to each other.

An active region or channel for printed, organic or plastic electronics or polymer semiconductors, such as organic field-effect transistors (OFETs), is obtained by using an enhanced inkjet drop-cast printing technique. A two-liquid system is employed to achieve the direct growth of well-oriented organic crystals at the active region of channel. High-performance electrical properties exhibiting high carrier mobility and low threshold voltage are obtained due to the proper orientation of molecules in the grown crystal in a highest mobility direction, due to the absence of grain boundaries, and due to low trap densities. The hydrophobic-hydrophilic interactions between the liquids utilized, which results in the fabrication of low-cost and mass-producible printable electronic devices for applications in flexible displays, electronic signages, photovoltaic panels, membrane keyboards, radio frequency identification tags (RFIDs), electronic sensors, and integrated electronic circuits.

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

The chromatographic separation material is a cyclofructan or a derivative of cyclofructan covalently bonded to a cross-linked, organic polymer. The separation material works well in hydrophilic interaction liquid chromatography.

The object of the present invention is to provide a packing material and a separation method that manifest excellent hydrophilic interactions.

The present invention provides a packing material for hydrophilic interaction chromatography consisting of a modified support treated with the surface modifier represented by the following formula (6) or (7).

(In this formula, m denotes 2-6 and n denotes 1-4. X₁, X₂, and X₃, independent of each other, denote a methoxy group, ethoxy group, or halogen. Up to two of X₁, X₂, and X₃ can be any of the following groups: a methyl group, ethyl group, propyl group, isopropyl group, butyl group, or isobutyl group.)

The invention discloses a phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use for enriching glycoprotein. The microsphere orderly comprises amagnetic core, a connecting layer, a metal organic skeleton layer and a phenylboronic acid outer layer from inside to outside, has a large specific surface area and good hydrophilicity, contains a large number of functional groups of boric acid and can specifically enrich glycoprotein through hydrophilic interaction and boric acid affinity.

The invention discloses an ultrathin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment. A glycopeptide enrichment material utilizes anultrathin two-dimensional lamellar structure molybdenum disulfide nano-material as a matrix. After surface modification with nano-gold, through a simple and stable Au-S bond chemical reaction, a mercapto-containing glycopeptide enrichment functional group is loaded. An experimental result shows that the functionalized molybdenum disulfide-nano-noble metal composite material and glycopeptides produce hydrophobic interaction or through a reversible reaction of the boronic acid group supported on the surface of the material and the sugar chain of the glycopeptides, the functional material realizes the highly selective enrichment of glycopeptides. The functionalized molybdenum disulfide-nano-noble metal composite material is prepared through simple processes under mild reaction conditions.

In one aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a protein, peptide, or amino acid includes providing a column having an anion-exchange material at a pH of less than about 4, and eluting the compound using a mobile phase comprising an amount of organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction. In another aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a nucleic acid or nucleotide comprises providing a column having a cation-exchange material at a pH of less than about 3.4, and eluting the compound using a mobile phase comprising organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction.

The invention relates to the technical field of liquid chromatography, in particular to an online hydrophilic interaction chromatography/reversed phase chromatography serial interface device. The online hydrophilic interaction chromatography/reversed phase chromatography serial interface device comprises a high-pressure inert gas source and relevant pressure reduction and valve switching devices. Specifically, one end of a first-dimensional chromatographic column is connected with an inert gas (such as nitrogen) pressure reducing valve and a pump system through a switching valve and pipelines, and the other end of the first-dimensional chromatographic column is connected with a second-dimensional chromatographic column and waste liquid through a switching valve and pipelines; by switching the valves, the high-pressure inert gas replaces a solvent left in the first-dimensional chromatographic column, the problem of incompatibility of solvents in online series connection is solved, reservation of samples on the second-dimensional chromatographic column is guaranteed, and therefore hydrophilic interaction chromatography and reversed phase chromatography are connected in series. An interface is convenient to use, simple in operation, capable of saving time, efficient and high in universality; due to the fact that the hydrophilic interaction chromatography and the reversed phase chromatography are directly connected in series, the samples can be online, quickly and continuously operated, and losses of the samples are reduced.

A sol-gel sorbent or chromatography stationary phase is a particulate metal oxide gel containing polymeric segments uniformly distributed throughout the metal oxide gel. The metal oxide gel is an oxide from silicone or other metal oxide that can have one of the valence bonds attached to an organic group and the remainder occupied by oxygens that can be provided as an oxide or an alkoxide or aryl oxide of the polymeric segments. The particles are used for an SPE sorbent or as a packing for a reversed phase high-performance liquid chromatography (RP-HPLC), a normal phase high-performance liquid chromatography (NP-HPLC) column or a hydrophilic interaction liquid chromatography (HILIC) column.

The invention discloses a surface polymerized ionic liquid modified magnetic nanometer material as well as a preparation method and application thereof. The surface polymerized ionic liquid modified magnetic nanometer material is prepared from magnetic nanoparticles, a silicon dioxide layer cladding the surfaces of the magnetic nanoparticles and a hydrophilic ionic liquid layer which is polymerized on the surface of the silicon dioxide layer through a mode of modifying a silane coupling agent with carbon-carbon double bonds at terminal on the surface of the silicon dioxide layer for precipitation polymerization. With adoption of the surface polymerized ionic liquid modified magnetic nanometer material, through a mode of precipitation polymerization, the ionic liquid is polymerized on the surfaces of the magnetic nanoparticles; a material synthetic method is simple, clean and rapid; through the hydrophilic interaction and electrostatic interaction of the polymerized ionic liquid and glycopeptide, efficient selective enrichment of the glycopeptide is realized; the glycopeptide enrichment effect is good, and glycopeptide enrichment detection in human serum albumin (such as IgG) with low concentration can be realized.

The invention discloses a preparation method and application of a polar bridging cyclodextrin chiral monolithic column, and belongs to the field of analytical chemistry. The monolithic column is prepared by the following steps: dissolving cyclodextrin with N, N-(1, 2-dihydroxyethylene) diacrylamide DAA in deionized water, reacting under the condition of an initiator AIBA to generate polar bridgingcyclodextrin, adding urea and formaldehyde, and quickly preparing the polar bridging cyclodextrin chiral monolithic column within 30 minutes by virtue of a one-pot thermal polycondensation method. The preparation method is simple and rapid in reaction, a cyclodextrin bimolecular bridging structure is constructed by adopting a bridging agent with high polarity; the additional hydrophilic interaction can be provided, the chiral recognition ability of the stationary phase can be significantly enhanced, the excellent resolution ability on a variety of amino acids, RS-(+)-(4-methoxybenzene) ethylamine and other different types of drugs can be displayed in the electrochromatography, and the method can be used as the chromatographic chiral fixation so as to be correspondingly used in the field of drug chiral resolution.

The invention provides a chromatography-mass spectrometry detection method for electrophoresis hydrophilic interaction of sulodexide and belongs to the technical field of detection of medicines, crudedrugs and raw materials. According to the method provided by the invention, the difference of electrophoretic mobility of each component in the sulodexide is utilized and the components are separatedthrough agarose gel electrophoresis; then the components are extracted by utilizing an ion exchange centrifugal column; then each component is enzymolyzed into a composition unit by utilizing the specificity of an enzyme; finally, each component of the sulodexide is detected through a chromatography-mass spectrometry method for the hydrophobic interaction, so that qualitative and relatively quantitative analysis is carried out on rapid mobile heparin (FMH), slow mobile heparin (SMH) and dermatan sulfate (DS) of the sulodexide, and a fine structure and a terminal structure of each component are identified and analyzed.