10 results about "Hydrophilic Interactions" patented technology

The invention discloses a method for detecting natural plant hormones and exogenous growth regulators of vegetables, efficient separation and accurate detection of the natural plant hormones and the exogenous growth regulators are realized through combination of hydrophilic interaction chromatography and mass spectrometry, a hydrophilic interaction chromatographic column is adopted, and conditions such as mobile phase composition and flow velocity are optimized; the selectivity of polar substances is improved by utilizing hydrophilic interaction, so that compounds with similar structures achieve baseline separation, and chromatographic peak overlapping interference is avoided; the mass spectrometric detection only responds to a specific ion signal by screening characteristic parent ion / daughter ion pairs of a target object, so that matrix impurities and non-target ion interference are effectively eliminated, the false positive risk is remarkably reduced, and the detection sensitivity and specificity are improved; the combined technology is combined with the efficient separation capacity of the hydrophilic interaction chromatographic column and a multi-reaction monitoring mode, synchronous separation of multiple compounds with similar polarities in single analysis is achieved, parallel detection of dozens of target objects is supported, and the analysis time is greatly shortened.

The invention relates to a method for quantitatively detecting ion residues in an mRNA (messenger Ribonucleic Acid) composition, in particular to a method for detecting metal ions by UPLC-CAD (Ultra Performance Liquid Chromatography-Computer Aided Design) operated in a HILIC (Hydrophilic Interaction Chromatography) mode. According to the method, liquid chromatography (HILIC) suitable for hydrophilic interaction is adopted, an unbonded silica gel column and an electrospray detector (CAD) are selected, and acetonitrile-water is used as a mobile phase. The method is accurate, reliable, good in chromatographic separation degree and high in sensitivity, and can simultaneously realize limit separation of four targets of sodium, potassium, magnesium and calcium in the mRNA solution.

The invention relates to column packing and a preparation method thereof. In particular, the present invention relates to a column packing for hydrophilic interaction liquid chromatography (HILIC).

The invention discloses a preparation method and application of a strong hydrogen bond driven nitrogen-oxygen high-hydrophilic material, tetraethoxysilane (TEOS) and 3-(2-(2-aminoethylamino) ethylamino) propyl-trimethoxysilane (3AMPTS) are used as silicon sources, a mixed microemulsion of a surfactant, a cosurfactant, an oil phase and a basic catalyst is added, and the strong hydrogen bond driven nitrogen-oxygen high-hydrophilic material is prepared by using a reversed-phase microemulsion method. And the polyamino nano silicon spheres with uniform particle size and controllable functional group content are prepared. And carrying out dimethylation on the nano silicon spheres by using formaldehyde, and further oxidizing the dimethylated silicon spheres to obtain the hydrophilic silica gel material containing N-O groups. According to the material disclosed by the invention, strong hydrogen bond interaction between a proton receptor N-O group in a hydrophilic material and a proton donor such as-OH group in glycopeptide is utilized, so that the hydrophilic interaction force of the material on the glycopeptide is enhanced, and the enrichment capacity of the glycopeptide is improved.

A microfluidic chromatographic column comprising a stationary phase substrate and a method for functionalizing a chromatographic column. The stationary phase substrate has a liquid channel defined by channel walls, and the channel has an inlet and an outlet. A strut is positioned in the channel on the substrate. A stationary phase substrate of a chromatographic column may comprise silicon oxide. At least a portion of the surface of the stationary phase substrate may be attached to the linker, and the linker is attached to the polymer.

The present invention relates to column packing material and methods of making thereof. In particular, the present invention relates to column packing material that is used in Hydrophilic Interaction Liquid Chromatography (HILIC).

The present invention relates to a column packing material and a method for producing the same. Specifically, the present invention relates to a column packing material used in hydrophilic interaction liquid chromatography (HILIC).

Disclosed herein are embodiments of methods for oligonucleotide analysis using a novel solid-phase extraction and hydrophilic-interaction liquid chromatography. The unique polar-based retention methods provided herein provide a high-recovery extraction. The methods improve assay reliability and reproducibility and reach picomolar sensitivity with the demonstrably beneficial accurate mass platform. Also disclosed herein are systems and computer program products for performing these methods.

The present disclosure is directed to methods of separating one or more non-modified oligosaccharide(s) with degree of polymerization 6 or higher from a sample using a hydrophilic interaction liquid chromatography (HILIC) column connected to a liquid chromatography system that includes coated flow paths. The methods disclosed herein requires, inter alia, a liquid chromatography system, wherein at least one component of the LC system includes a fluid-contacting alkylsilyl coating. When implementing a standard HILIC method, the methods of the present technology can be used to decrease the analyte loss and / or carry-over of the separation leading to improved analysis.

A method of processing of a biological sample containing multiple metabolites is described The method comprising the steps of pre-treating the biological sample with a metabolite extraction solvent to provide a pre-treated sample, separating a first aliquot of the pre-treated sample by reverse phase liquid chromatography (RPLC) to provide a first eluent containing resolved hydrophobic metabolites, and separating a second aliquot of the pre-treated sample by hydrophilic interaction liquid interaction chromatography (HILIC) to provide a second eluent containing resolved hydrophilic metabolites. The first and second eluents are assayed using targeted tandem mass spectroscopy operated in multiple reaction monitoring mode. Each liquid chromatography step(LC) is directly hyphenated with the tandem mass spectrometry (MS / MS) into a single LC-MS / MS analysis. The extraction solvent typically comprises methanol, isopropanol and an acetate buffer.