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111 results about "Hydrophilic interaction chromatography" patented technology
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Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Dr. Andrew Alpert in his 1990 paper on the subject. He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re-evaluation of published data.
Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
ActiveUS20150204824A1High resolutionDesirable retentivityComponent separationSurface/boundary effectMaterial DesignGlycan
The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra / inter-particle diffusion distances.
Owner:WATERS TECH CORP
Separation Of Glycans By Mixed-mode Liquid Chromatography
An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and / or weak cation exchange binding sites in combination with one or more reverse phase and / or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.
Owner:DIONEX CORP
Purification of proteins using hydrophobic interaction chromatography
InactiveUS20150299249A1Improve purification effectPromote recoveryImmunoglobulins against cytokines/lymphokines/interferonsPeptide preparation methodsStrong bindingImpurity
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.
Owner:ABBVIE INC
Hilic / anion-exchange / cation-exchange multimodal media
ActiveUS20140370614A1Easy to synthesizeUnique selectivityChromatographic cation exchangersComponent separationDiagnostic Radiology ModalityAnalyte
Owner:DIONEX CORP
Am imidazole dicationic ionic liquid hydrophilic interaction chromatography stationary phase, and preparation and applications thereof
InactiveCN104689807ACation exchanger materialsOther chemical processesChemical reactionClick chemistry
The invention relates to an imidazole dicationic ionic liquid-bonded silica gel hydrophilic interaction chromatography stationary phase, and provides a preparing method of the stationary phase. Firstly, imidazole dicationic ionic liquid with N-terminal alkenyl is prepared, 3-mercaptopropyl is grafted to surfaces of silicon spheres, and the imidazole dicationic ionic liquid with the N-terminal alkenyl is boned to the surfaces of the silicon spheres modified with the 3-mercaptopropyl by utilization of a mercapto-alkenyl click chemical reaction. The stationary phase has good retention and separation selectivity for typical hydrophilic compounds such as nucleosides, nucleic acid bases and organic acids, is ideal in column efficiency which can reach 130000 / m, and shows a good application prospect as a hydrophilic interaction chromatography stationary phase.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra / inter-particle diffusion distances.
Owner:WATERS TECH CORP
Hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis
ActiveCN106153763ASimple and fast operationEasy to trainComponent separationLipid formationPhospholipid
The invention discloses a hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis. The method includes the steps of firstly, performing corresponding preprocessing on ground Metapenaeus ensis to obtain crude lipid extract; secondly, performing liquid phase separation on the crude lipid extract, wherein a chromatographic column is a YMC Triart diol HILIC column, a gradient elution method is used, a gradient system comprises a flowing phase A and a flowing phase B, the flowing phase A is an acetonitrile solution containing 53mmol / L formic acid, and the flowing phase B is an aqueous solution containing 60mM ammonium formate and 53mM formic acid; thirdly, performing mass spectrometric detection analysis on the elution liquid obtained in the second step. By the method, the total content of PE, PS and PC in the Metapenaeus ensis can be detected accurately, and mass spectrometry identification of various molecular species in the PC, PE and PS phospholipids can be achieved.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
Chitosan oligosaccharide hydrophilic interaction chromatography stationary phase and preparation method thereof
InactiveCN101757897AEfficient separationSimple structureOther chemical processesSolid sorbent liquid separationClick chemistrySilica gel
The invention relates to a preparation method for a chitosan oligosaccharide hydrophilic interaction chromatography stationary phase. The click chemistry is adopted as a bonding reaction method for bonding chitosan oligosaccharide, firstly, terminal alkynyl is introduced on the surface of silica gel, secondly, the following solvents of water and methanol or the mixture of the solvents is taken as the reaction solvent, and then the chitosan oligosaccharide modified with azido group is bonded to the surface of the silica gel to obtain the chitosan oligosaccharide hydrophilic interaction chromatography stationary phase; the chitosan oligosaccharide is adopted as the polarity functional group with simple structure, and the chitosan oligosaccharide taken as the polarity stationary phase can realize the highly effective separation to the strongly polar compounds under the hydrophilic interaction liquid chromatogram mode; the chitosan oligosaccharide taken as the functional group has stable property, the surface structure does not change due to the change of pH, and the chitosan oligosaccharide is not liable to react with the solute molecule; and the click chemistry is adopted as the bonding reaction method, therefore, the purpose of immobilization with high selectivity and high transformation ratio can be achieved under the mild conditions.
Magnetic nano material and preparation and application thereof
InactiveCN105771942AImprove hydrophilicityEasy to separateOther chemical processesPreparing sample for investigationChemistryNanomaterials
The invention relates to a pretreatment process of a glycosylated protein sample, in particular to a novel magnetic nano material for pretreatment of the glycosylated protein sample.The method comprises the steps of preparation of the magnetic nano material, subsequent modification of glycopeptide dendritic macromolecules, and enrichment of glycosylated polypeptide with the hydrophilic interaction chromatography.The method is high in sensitivity and suitable for pretreatment of glycosylated protein in a biological sample.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Separation of glycans by mixed-mode liquid chromatography
ActiveUS20140178912A1Highly versatileDesirable selectivitySilicon organic compoundsComponent separationChromatographic separationBinding site
An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and / or weak cation exchange binding sites in combination with one or more reverse phase and / or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.
Owner:DIONEX CORP
Honey adulteration detection method based on HPLC-ELSD and partial least squares discriminant analysis method
The invention discloses a honey adulteration detection method based on HPLC-ELSD and a partial least squares discriminant analysis method, and belongs to the technical field of food detection. The method comprises simple sample pretreatment, wherein the simple sample pretreatment comprises the steps of adopting a hydrophilic interaction chromatography separation-evaporation light scattering detector (HILIC-ELSD) method to analyze oligosaccharide and isomer components of oligosaccharide in authentic honey and a honey sample adulterated with fructose corn syrup or malt syrup, and utilizing difference of oligosaccharide component fingerprint spectrums of the authentic honey and the adulterated honey to conduct data processing through the partial least squares discriminant analysis method, so that adulteration identification is further achieved. The honey adulteration detection method based on the HPLC-ELSD and the partial least squares discriminant analysis method can effectively identify adulterated honey with C4 botanical syrup or C3 botanical syrup, the adopted analysis instrument is popular, and the method is convenient for popularization and application.
Owner:JIANGNAN UNIV
Identification method for O-glycosylation peptide fragment and complete saccharide chain thereof
ActiveCN105467050AHigh sensitivityEliminate distractionsComponent separationMass spectrometryDigestion
The present invention relates to an identification method for an O-glycosylation peptide fragment and a complete saccharide chain thereof. The technical process specifically comprises that a glycosylation protein sample is subjected to PNGase F digestion to remove N-glycosylation modification interference, enrichment is performed through hydrophilic interaction chromatography, and finally analysis is performed through time of flight mass spectrometer. According to the present invention, the method process has advantages of simple and rapid operation, high identification sensitivity, and the like; and the O-glycosylation peptide fragment and the complete saccharide chain thereof of the complex biological sample can be identified so as to achieve the research on the micro-uniformity of the O-glycosylation modification structure.
Owner:ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI +1
Core-shell type hydrophilic chromatographic stationary phase with metal organic framework material as shell, preparation method and application thereof
ActiveCN105148882AAchieving Hydrophilic Interaction Chromatographic SeparationsEvenly distributedComponent separationOther chemical processesStationary phaseChromatographic separation
The invention provides a core-shell type hydrophilic chromatographic stationary phase with a metal organic framework material as the shell, a preparation method and application thereof. The stationary phase takes the metal organic framework prepared with an imidazole ionic liquid as the organic ligand to modify spherical silica gel particles, has wide application range, and can realize hydrophilic interaction chromatography separation analysis of most polar compounds. At the same time, the preparation method of the core-shell type hydrophilic interaction chromatography stationary phase provided by the invention has the advantages of simple preparation process, mild reaction conditions, good preparation reproducibility, and easy popularization and use.
Owner:HEBEI UNIVERSITY
HILIC/anion-exchange/cation-exchange multimodal media
Owner:DIONEX CORP
Packing Material For Hydrophilic Interaction Chromatography
InactiveUS20110100915A1Easy to separateSufficient retention timeIon-exchanger regenerationGlass/slag layered productsHalogenFilling materials
The object of the present invention is to provide a packing material and a separation method that manifest excellent hydrophilic interactions.The present invention provides a packing material for hydrophilic interaction chromatography consisting of a modified support treated with the surface modifier represented by the following formula (6) or (7).(In this formula, m denotes 2-6 and n denotes 1-4. X1, X2, and X3, independent of each other, denote a methoxy group, ethoxy group, or halogen. Up to two of X1, X2, and X3 can be any of the following groups: a methyl group, ethyl group, propyl group, isopropyl group, butyl group, or isobutyl group.)
Owner:SHISEIDO CO LTD
Method for purifying and separating oligosaccharides
InactiveCN102101875AAddressing Insufficient SelectivityHigh selectivitySugar derivativesDisaccharidesStationary phaseElution
The invention discloses a method for purifying and separating oligosaccharides. A polar chromatographic column is prepared by taking a polar filler as a chromatographic stationary phase in the chromatographic mode of a hydrophilic interaction chromatography principle, wherein water, acetonitrile and a buffer salt serve as a mobile phase; water serves as a strong elution solvent; and an isocratic or gradient elution condition is used. In the method, parameters such as the type and concentration of the buffer salt, the gradient of the mobile phase, column temperature and the like are optimized, so that separation and preparation of acid, neutral and alkaline oligosaccharides are realized. The method has the characteristics of high stability, large loading amount, easiness, convenience and controllability in operating and the like, and is suitable for separating and purifying different types of oligosaccharides.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Method for extracting daptomycin from fermentation broth
InactiveCN102492024AReduce acidolysisGood medical market prospectPeptide preparation methodsSodium acetateIon exchange
A method for extracting daptomycin from fermentation broth relates to fermentation broth. Supernatant liquor is obtained after the fermentation broth is centrifuged, the daptomycin enters an organic phase after the supernatant liquor is extracted by an organic solvent, sodium acetate-acetic acid buffer solution is used to perform re-extraction to reach a water phase so as to obtain strip-extraction liquid, the strip-extraction liquid is purified through hydrophobic interaction chromatography, isopropanol-sodium acetate-acetic acid buffer solution with constant intensity is used for elution to obtain hydrophobic interaction chromatography eluent, the eluent is separated and purified through ion-exchange column chromatography to obtain ion-exchange eluent, the sodium acetate-acetic acid buffer solution is used as basic liquid, and neutral salt is added for elution so as to complete extraction of the daptomycin from the fermentation broth. Organic solvent extraction and resin chromatography technology is applied comprehensively, and the daptomycin with purity more than 85% can be separated and purified from the fermentation broth by using the method. Compared with other separating and purifying technology, the method is moderate in operating condition, low in cost and capable of effectively reducing acidolysis of the daptomycin. The daptomycin serving as novel lipopeptide antibiotics has good medical market prospect.
Owner:XIAMEN UNIV
Organic-inorganic hybrid microsphere particles, and preparation and application thereof
ActiveCN105732916AGood reproducibilityPreparation reaction conditions are mildOther chemical processesAlkali metal oxides/hydroxidesMicrosphereClick chemistry
The invention relates to the field of polymer material and analysis, and relates to organic-inorganic hybrid core-shell-structured microsphere particles, and a preparation and an application thereof. According to the invention, inorganic silica gel particles are adopted as cores, and are modified with a silanization agent; with a thiol-ene click chemistry technology, 3-allyloxy-2-hydroxy-1-propanesulfonic acid and methylene diacrylamide are wrapped on the surfaces of the cores, such that the organic-inorganic hybrid core-shell-structured nano / micro particles with smooth surface and with hydrophilic functional groups such as hydroxyl group, sulfonic acid group, amide and the like are formed. According to the invention, the hydrophilic functional groups such as hydroxyl group, sulfonic acid group, amide and the like are introduced to the surface of the material through the thiol-ene method, such that the defects of complicated steps and low reaction efficiency of a traditional post-modification method are solved, and a polymer three-dimensional-structured hydrophilic layer is formed. Better advantage is shown when the particles are used in hydrophilic chromatography. The microsphere particles can be used in hydrophilic interaction chromatography for separating and enriching glycopeptide, and have good practical value and application prospect in the fields of separation analysis and sugar proteomics.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Intelligent response liquid chromatogram filling material and preparation method thereof
InactiveCN105536747AHigh mechanical strengthImprove physical stabilityOther chemical processesPhenylboronic acidN isopropyl acrylamide
The invention discloses an intelligent response liquid chromatogram filling material. The filling material is made by wrapping silicon balls with an intelligent response material, and the intelligent response material is co-polymer of N-isopropyl acrylamide, 4-(trifluoromethyl)-phenylthiourea-2-acrylamide and 3-acrylamide phenylboronic acid. The filling material can achieve the effect that the intelligent response material is switched from the super hydrophilicity to the super-hydrophobicity through excitation by way of one or more of the temperature, the pH value and carbohydrate so that and the the synthesized filling material can achieve the separation mode of reversion phase chromatography and hydrophilic chromatography of hydrophobic substances, hydrophilic substances and other substances through control over such conditions as the temperature, the pH value and sugar. The invention further discloses a preparation method of the intelligent response liquid chromatogram filling material.
Owner:CHONGQING UNIV
Sulfydryl Michael addition reaction-based silica gel matrix chromatography separation material and preparation thereof
ActiveCN106622182ANovel structureThe preparation process is simple and reliableOther chemical processesChromatographic separationReversed-Phase Liquid Chromatography
The invention relates to a silica gel matrix high-performance liquid chromatography separation material. An electron deficient alkene monomer and sulfydryl silica gel are subjected to sulfydryl Michael addition to form a hydrophilic interaction chromatography stationary phase, a reversed phase chromatography stationary phase and an affinity chromatography stationary phase, and the structural formula is as shown in the description, wherein SiO2 is mesoporous silica gel or non-porous silica gel, R-X represents one of formulas as shown in the description, and X represents one of a polar functional group, a non-polar functional group or an affinity functional group. The chromatography separation material provided by the invention has a novel structure, contains a characteristic thioether functional group, also contains an amide, ester, sulfone or succinimide functional group, and can serve as a stationary phase of reversed phase chromatography, hydrophilic interaction chromatography or affinity chromatography to provide different action forces and improve the separation selectivity of the chromatography material. A preparation method provided by the invention is simple and efficient, and the obtained stationary phase has the advantages of large bonding amount, high stability and the like.
Owner:ACCHROM TECH (DALIAN) TECH CO LTD
Rapid detection method for melamine and sulbactam in liquid milk
ActiveCN106018621AReduce experiment costReduce processing costsComponent separationRelative standard deviationLiquid milk
The invention relates to a rapid detection method for melamine and sulbactam in liquid milk, and belongs to the technical field of liquid milk analysis. In the rapid detection method, a sample is extracted through an acetic acid water solution, subjected to protein sedimentation through acetonitrile and diluted, Acquity UPLC BEH HILIC(100*2.1mm, i.d., particle diameter of 1.7 micrometers) separation is carried out, and electrospray ion source multi-reaction monitoring (MRM) mode tandem mass spectrum measurement is carried out. An extracting solvent, extracting time, purifying time, instrument measurement conditions and the like are optimized. Results prove that melamine and sulbactam have the good linear relation within the range of 0.02-20 ng / mL and 0.05-100 ng / mL respectively, and the correlation coefficient is larger than 0.99. In the high, middle and low addition levels, the recovery rate of practical samples is between 82.5% and 103.5%, the relative standard deviation (RSD n=6) is between 0.9% and 5.1%, and the method quantification limit of melamine and sulbactam is 10 micrograms per kilogram and 1.0 microgram per kilogram respectively. Pretreatment operation is simple and rapid, hydrophilic interaction chromatography-tandem mass spectrometry detection sensitivity is high, and qualitative and quantitative operation is accurate.
Owner:SHANDONG INST FOR FOOD & DRUG CONTROL
Separation of Charged Solutes by Electrostatic Repulsion-Hydrophilic Interaction Chromatography
InactiveUS20080220532A1Component separationSolid sorbent liquid separationStationary phaseOrganic solvent
In one aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a protein, peptide, or amino acid includes providing a column having an anion-exchange material at a pH of less than about 4, and eluting the compound using a mobile phase comprising an amount of organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction. In another aspect, a method of performing electrostatic repulsion-hydrophilic interaction chromatography on a nucleic acid or nucleotide comprises providing a column having a cation-exchange material at a pH of less than about 3.4, and eluting the compound using a mobile phase comprising organic solvent sufficient to substantially balance the electrostatic repulsion of the stationary phase with hydrophilic interaction.
Owner:POLYLC
Method for detecting content of L-hydroxyproline in dairy product
InactiveCN102590420AOvercoming poor specificityRich structural informationComponent separationChromatography columnTandem mass spectrometry
The invention discloses a method for detecting the content of L-hydroxyproline in a dairy product. The method comprises the following steps of: 1) pre-treating a sample, namely adding acid into the diary product and hydrolyzing to obtain test liquid; 2) analyzing the content of L-hydroxyproline by liquid chromatogram-tandem mass spectrometry, wherein hydrophilic interaction chromatography (HILIC) is adopted, a flowing phase is a mixed solution a flowing phase A and a flowing phase B, and the flowing phase A is a 10mM ammonium acetate aqueous solution of which the pH is 5.6; and the flowing phase B is acetonitrile, and the mass spectrometry condition is that: an electrospray ion source is adopted, a scanning mode is a positive ion scanning mode, a detection mode is a multi-reaction detection mode, electrospray voltage is 5,500V, the temperature of the ion source is 600 DEG C, the pressure force of a gas curtain is 20Psi, the atomization pressure force is 50Psi, and auxiliary pressure force is 30Psi. By the method, the detection accuracy and sensitivity can be improved obviously, and the content of the L-hydroxyproline in the dairy product can be determined easily, conveniently and quickly.
Owner:上海德诺产品检测有限公司
Method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously
ActiveCN107991399AReliable methodThe determination method is accurate and reliableComponent separationMedicinal herbsAnalyte
The invention relates to a method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously. The method adopts a direct series-reverse phase-hydrophilic interaction chromatography mass spectrometer combination method and comprises steps as follows: step one, preparing a sample solution; step two, preparing series of standard solutions; step three, preparing an internal standard solution; step four, preparing a solution for test products; step five, injecting the solution obtained in the step four into a direct series-reverse phase-hydrophilic interaction liquid chromatography mass spectrometer combination system so as to obtain an extracted ion flow diagram of 31 analytes, and calculating content of the 31 components in the compound radix salviae miltiorrhizae extract or the related medicinal materials according to the extracted ion flow diagram.
Owner:TIANJIN TASLY PHARMA CO LTD
Purification of plasmid DNA by hydrophobic interaction chromatography
The invention refers to a new process for the production of purification of high purity plasmid DNA. The process comprehends: a) the production of cells containing plasmid DNA, b) the disruption of the cells in order to obtain a lysate containing plasmid DNA, c) a concentration step by precipitation with isopropanol, d) a pre-purification and conditioning step by the addition of ammonium sulphate, e) a purification step using hydrophobic interaction chromatography, f) a final concentration and / or buffer exchange step. The process is scaleable, it does not use enzymes or mutagenic agents and it enables the preparation of plasmid DNA with pharmaceutical grade, which complies with the requirements of regulatory agencies. The invention belongs to the technical domain of Biochemical Engineering / Biotechnology.
Owner:INSITUTO SUPERIOR TECNICO +1
Ergothioneine detection method
ActiveCN104749263AImprove retention behaviorSuitable for separation detectionComponent separationQuantitative determinationColumn temperature
The invention relates to a high pressure liquid chromatography detection method for ergothioneine. According to the invention, an hydrophilic interaction chromatography (HILIC) analysis chromatographic column is employed for performing a high pressure liquid chromatography analysis on a sample containing ergothioneine, the chromatographic column employs a HILIC filler as a fixed phase, a solution of acetonitrile and water (v / v) with ratio of (80-95): (20-5) is taken as a mobile phase, the pH value of the solution is 5.6-8.0, and the column temperature is 25-45 DEG C. the invention also relates to a quantitative determination method of ergothioneine.
Owner:TIANJIN SINONOCY BIOLOGICAL TECH CO LTD
Hydrophilic interaction chromatographic stationary phase based on amide functional imidazole ionic liquid and preparation method and application of hydrophilic interaction chromatographic stationary phase
InactiveCN107029686ANovel structureHigh column efficiencyOther chemical processesSolid sorbent liquid separationChemical reactionClick chemistry
The invention discloses a hydrophilic interaction chromatographic stationary phase based on amide functional imidazole ionic liquid and a preparation method and application of the hydrophilic interaction chromatographic stationary phase, and belongs to the field of high performance liquid chromatography stationary phases. Ionic liquid is bonded to a 3-sulfydryl propyl modified silicon dioxide ball surface to prepare the hydrophilic interaction chromatographic stationary phase through a sulfydryl-double bond clicking chemical reaction. The preparation method comprises the steps that firstly, the amide functional imidazole ionic liquid of terminal alkenyl is prepared firstly, the silicon dioxide ball surface is grafted with 3-sulfydryl propyl groups through a silanization reaction, and the ionic liquid is bonded to the silicon ball surface modified by 3-sulfydryl propyl. The surface bonded amide imidazole ionic liquid serves as the hydrophilic interaction chromatography stationary phase to show good reservation and selectivity on typical hydrophilic compounds, and the column efficiency amounts to that of a commercial amide hydrophilic interaction chromatography stationary phase. In addition, due to the unique multiple interactions of the hydrophilic interaction chromatographic stationary phase, the stationary phase has especially good reservation and separation selectivity on flavonoid matter under the hydrophilic chromatography mode, and good application prospects are achieved.
Owner:DALIAN UNIV OF TECH
Online hydrophilic interaction chromatography/reversed phase chromatography serial interface device and application thereof
ActiveCN104076113AEasy to operateAchieve removal of HILICComponent separationReversed-Phase Liquid ChromatographyNitrogen
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Hydrophilic interaction chromatography/ ion exchange chromatography mixed stationary phase and preparation and application thereof
ActiveCN106552610AStable structureRaw materials are easy to getCation exchanger materialsOrganic anion exchangersHybrid typeStationary phase
The invention relates to high-performance liquid chromatography stationary phases, in particular to a hydrophilic interaction chromatography / ion exchange chromatography mixed stationary phase and preparation and application of the hydrophilic interaction chromatography / ion exchange chromatography mixed stationary phase. The structure of the hydrophilic interaction chromatography / ion exchange chromatography mixed stationary phase is shown as the formula 1, and please see the formula 1 in the specifications, wherein X is -OCH3 or -OCH2CH3, Y1 is F or Cl or Br or I or BF4 or Tf2N or CF3SO3 or SCN or NO2 or NO3, Y2 is -NHCH3 or -N (CH3)2 or -N+ (CH3)3Y1 or -NHCH2CH3 or -N (CH2CH3)2 or -N+ (CH2CH3)3Y1, and n=2 or 3. According to the prepared mixed type stationary phase, raw materials are easy to obtain and the preparation procedure are simple; and the prepared mixed type stationary phase has good retaining performance and separation selectivity for multiple saccharides and has good application and popularization prospects.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Laurinol polyoxyethylene ether fully two-dimensional separation analysis method
The invention discloses a laurinol polyoxyethylene ether fully two-dimensional separation analysis method including the following steps: an ultrahigh pressure hydrophilic interaction chromatography-ion mobility mass spectrometry coupled method is adopted, a laurinol polyoxyethylene ether fully two-dimensional separation analysis system is constructed, and effective separation and accurate determination of a complex target compound system are realized; the method includes optimization of ultrahigh hydrophilic interaction chromatography conditions and ion mobility mass spectrometry conditions. According to the laurinol polyoxyethylene ether fully two-dimensional separation analysis method, through the ultrahigh pressure hydrophilic interaction chromatography-ion mobility mass spectrometry coupling technique, the laurinol polyoxyethylene ether fully two-dimensional separation analysis method is developed, and rapid separation detection of laurinol polyoxyethylene ether is realized.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
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