32results about How to "The determination method is accurate and reliable" patented technology

The present invention provides a method for detecting concentration of hydroxyl radical. In the system produced by adding capture agent terephthalic acid sodium salt (NaTA) into hydroxyl radical, 2-hydroxyl terephthalic acid (HTA) is produced by reaction of terephthalic acid sodium salt and hydroxyl radical, which has stable fluorescence and is detected by fluorescence spectrophotometer (excited wavelength lambada ex=315 nm, projecting wavelength lambada em=425 nm), and then the concentration of 2-hydroxyl terephthalic acid is conversed to the concentration of hydroxyl radical. The detecting method of present invention is easy to do, credibility and nicety, especial for used in the conditions of circumstance produced hydroxyl radical at the value of pH<12, or the solution of the hydroxyl radical produced system required the conductivity.

The invention discloses a method for detecting the free fatty acid content in a compound traditional Chinese medicine. By the method, the free fatty acid content in the compound traditional Chinese medicine is measured by high performance liquid chromatography; and a chromatographic signal is detected by an ultraviolet detector. Furthermore, the invention relates to a quality detection method for any one or more than one of linoleic acid, linolenic acid, palmitoleic acid, oleic acid, palmitic acid, stearic acid and palmitic acid. The method has the characteristics of high stability, high precision, wide linear range, wide application range and the like.

The invention discloses a method for measuring nitrofuran antibiotics in cosmetics. The method comprises the following steps: (1) pretreating a sample, namely adding the sample into a mixed solution of acetonitrile and methanol in a volume ratio of 70:30, uniformly mixing, performing ultrasonic extraction, centrifuging, airing supernatant till the supernatant is nearly dry, dissolving residues in a mixed solution of a 15mmol/L ammonium acetate solution and acetonitrile according to a volume ratio of 85:15, and sieving the solution through a micro-porous filter membrane with the size of 0.22 micron, thereby obtaining filtrate for later use; (2) performing high performance liquid chromatography separation on the filtrate, eluting a mobile phase by a gradient elution program, and quantifying according to an external standard method; and (3) confirming a detected positive sample by high performance liquid chromatography-mass spectrometry/mass spectrometry. According to the method, four nitrofuran antibiotics such as macrodantin, furazolidone, furaltadone and nitrofurazone are simultaneously detected. The method is accurate, reliable and high in sensitivity.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for determining vanadium in a silicon-vanadium alloy. According to the method for determining vanadium in a silicon-vanadium alloy, the vanadium is determined by a steel and alloy chemical analysis method-ammonium ferrous sulfate titration method, and hydrofluoric acid is added after concentrated hydrochloric acid is added. Through the invention, the defect of relatively low determination result is overcome, and an accurate method for determining vanadium in a silicon-vanadium alloy is obtained.

The invention discloses a method for simultaneously measuring the content of multiple active ingredients in eucommia ulmoides. The method adopts high performance liquid chromatography for analysis; the chromatographic condition is taking octadecylsilane bonded silica as a filler; the mobile-phase gradient elution process is: the mobile phase A is methanol, and the mobile phase B is an aqueous solution of phosphoric acid with mass concentration of 0.1%; the content of the mobile phase B is increased from 30% to 70% by mass according to the first-order linear gradient in 0.01-30.00min, reduced to 30% in 30.00-30.01min and kept at 30% in 30.01-40.00min; the flow rate is 0.8ml/min; the column temperature is 30 DEG C; the sample injection volume is 5mul; the detection wavelength detection process is: the 254nm wavelength is selected in 0.01-7.00min, the 277nm wavelength is selected in 7.00-12.50min, and the 254nm wavelength is selected in 12.50-40.00min; and multiple active ingredients include geniposidic acid, chlorogenic acid, pinoresinol diglucoside and rutin. According to the invention, the method is verified by linear relation survey, stability test, precision test, reproducibility test and sample injection recycling test, and the analysis method is proved to be accurate and reliable.

The invention belongs to an ion chromatograph sample injection volume measurement device and a sample injection volume measurement method, and aims at accurately measuring sampling volumes in full quantitative loop sample injection and partial quantitative loop sample injection of ion chromatographs. According to the ion chromatograph sample injection volume measurement device and the sample injection volume measurement method, a sample injection valve is utilized to ensure that inorganic salt solution and a quantitative loop, and flushing fluid and the quantitative loop are communicated withan ion chromatograph of a measurement solution passage or a flushing channel technical scheme in turn to carry out sample injection volume measurement; and by using the sample injection volume measurement method of the device, the technical problems that in existing ion chromatograph sample injection volume measurement devices and sample injection volume measurement methods, the gas flow rate is low to cause the fact that residual liquid exists in quantitative loops, the gas flow rate is high to cause the fact that certain liquid is brought away, and the measured liquid is easy to evaporate tocause loss and then influence the measurement correctness.

The invention provides a method for detecting newly increased detection components in a traditional Chinese medicinal Tang Herb for treating AIDS. The method for detecting newly increased detection components comprises the following steps: 1, discriminating Hedyotis diffusa and Medicine Terminalia Fruit components in the Tang Herb through adopting thin-layer chromatography; and 2, simultaneously detecting gallic acid, chlorogenic acid, liquiritin and picroside II through gradient elution of a mobile phase under same high performance liquid chromatographic conditions. The content of gallic acid in every tablet of Tang Herb is not less than 0.9 mg, the content of chlorogenic acid in every tablet of the Tang Herb is not less than 0.5 mg, the content of liquiritin in every tablet of the Tang Herb is not less than 0.25 mg, and the content of the picroside II in every tablet of the Tang Herb is not less than 0.45 mg. Full-inspection projects confirm that the newly increased detection component detection method can be added to an original detection method in order to further improve the detection kind and the detection method of a medicine and facilitate the batch production determination of the traditional Chinese medicine Tang Herb and the improvement of the product quality. The detection method provided by the invention has the advantages of good stability and reliability, high specificity and large application values.

The invention relates to an automatic synthetic information monitoring system for the underground liquid. The invention is characterized in that the system comprises an underground liquid collection system, a combinational electrode measure system, an automatic degassing and drying system, a sensor system, an automatic gas chromatography analysis system and an intelligent measure control and computer system. The underground liquid collection system is connected with the combinational electrode measure system and the automatic gas escaping and drying system; after being distributed by gas transmission pipes, the automatic gas escaping and drying system is respectively connected with the sensor system and the automatic gas chromatography analysis system; the combinational electrode measure system and the sensor system are connected with the intelligent measure control and computer system in a CAN main line way. The invention has the advantage that the invention is capable of obtaining all kinds of real-time monitoring information from the automatic gas chromatography analysis system, the sensor and the electrode system through one automatic sampling. The information comprises the synthetic information of the underground liquid, such as hydrogen, helium, oxygen, argon, nitrogen, methane, carbon dioxide, sulfur hydrogen, conductance, acidity and alkalinity, chlorine ion, fluorine ion and temperature etc.

The invention relates to a measurement method of N-methylpyrrolidine content of cefepime dihydrochloride material and relative agent, for evaluating the quality of cefepime dihydrochloride material and relative agent. The invention discloses a liquid chromatograph which comprises using carboxylic cationic column as analysis column, uses a conductive detector to check, preparing flow phase, preparing sample solution and reference substance solution, using test method to respectively inject the sample solution and reference substance solution into a liquid chromatograph, recording high pressure liquid chromatographs, and calculating the N-methylpyrrolidine content of the sample. The inventive chromatograph system has easy balance, short sample test time, better repeatability in preserved time, stable baseline, durable column, high quality of chromatograph peak of N-methylpyrrolidine, better repeatability, accurate, simple and reliable process.

The invention discloses a content determination method for sacubitri valsartan trisodium hemi-pentahydrate capsule effective ingredients. The content determination method comprises the following steps: firstly taking an appropriate amount of a sacubitri valsartan trisodium hemi-pentahydrate reference substance to prepare the sacubitri valsartan trisodium hemi-pentahydrate reference substance withthe concentration of 50 micrograms per mL, and taking a sacubitri valsartan trisodium hemi-pentahydrate capsule sample to prepare a sacubitri valsartan trisodium hemi-pentahydrate sample for test withthe concentration of 50 micrograms per mL; then establishing the following chromatographic conditions of a high performance liquid: a C18 chromatographic column (4.6 mm * 250 mm, 5 microns), the moving phase is 0.2% diisopropyl amine water solution-N-methyl pyrrolidone, the detection wavelength is 250 nm, the flow velocity is 0.8 mL/min, the column temperature is 30 DEG C, and the sample size is10 microliters; finally accurately absorbing the reference substance and the sample solution for test into a liquid chromatograph for determination respectively. According to the content determinationmethod for sacubitri valsartan trisodium hemi-pentahydrate capsule effective ingredient disclosed by the invention, the determination method is convenient, accurate and reliable, and the quality of the product can be really reflected.

The invention discloses a method for detecting the content of effective components in pirfenidone tablets, which comprises the following steps of: taking a proper amount of ground pirfenidone tablet sample, putting the ground pirfenidone tablet sample into a volumetric flask, adding a mobile phase, dissolving and diluting to obtain a test solution; taking a proper amount of a pirfenidone reference substance, placing the pirfenidone reference substance in a volumetric flask, and dissolving and diluting the pirfenidone reference substance with a mobile phase to obtain a reference substance solution; precisely measuring 10 microliters of the test solution and 10 microliters of the reference solution, respectively injecting the test solution and the reference solution into a high performance liquid chromatograph, and recording chromatograms, isocratic elution is carried out by using octadecylsilane chemically bonded silica as a filler and water-acetonitrile as a mobile phase, the determination method is convenient, accurate and reliable, and effective control on the quality of the pirfenidone tablet can be realized.

The invention provides a method for testing the content of sugar in fermentation liquor and application. The method comprises the following steps: performing centrifugal treatment on the fermentation liquor, diluting supernatant, adjusting the pH value, and testing the content of the sugar of the diluent by using a glucometer. The invention further provides the application of the method in testing the glucose content of the fermentation liquor, and preferably, the fermentation liquor includes fermentation liquor and microorganism fermentation liquor.

The invention relates to the technical field of chemical product testing, in particular to a measurement method of inorganic germanium content in carboxyethylated germanium sesquioxide (Ge-132). Hydrochloric acid is used for distilling and separating inorganic germanium (IV) ions in a carboxyethylated germanium sesquioxide solution, then phenylfluorone-CTMAB- is adopted on the separated inorganic germanium (IV) ions for developing, and a spectrophotometric method is used for measurement. The measurement method of the inorganic germanium content in carboxyethylated germanium sesquioxide is reliable.

The invention discloses a simple plant callus subculture weight increase determination method during plant tissue culture. The method includes: firstly, placing a piece of disinfected filter paper on a solidified culture medium, weighing, and dicing a to-be-subcultured callus and inoculating the diced callus on the filter paper prior to weighing, wherein the difference between two-time weighing results serves as initial weight; and after culture, sampling and weighing the grown callus, wherein a numerical value obtained by subtracting the initial weight from grown weight serves as increased weight during subculture. The culture medium supplies water and nutrients to the callus by the aid of a filter paper bridge, the callus is in non-sticky connection with the filter paper, is unattached to the culture medium, can be tweezed directly by tweezers and is less prone to crushing, and the method is simple, convenient, feasible, free of interference during weighing, accurate and reliable.

The invention discloses a method for determining the content of (2E, 4E)-ethyl-4-(pyridine-2-yl imino)-2-ethyl crotonate, which comprises the following steps of: preparing a (2E, 4E)-ethyl-4-(pyridine-2-yl imino)-2-ethyl crotonate reference substance into a reference substance solution with the concentration of 5mu g/mL, and measuring the content of the (2E, 4E)-ethyl-4-(pyridine-2-yl imino)-2-ethyl crotonate. Preparing a minodronic acid hydrate test solution with the concentration of 500mu g/mL from a raw material medicine sample of the minodronic acid hydrate, and finally, precisely sucking the reference substance solution and the test solution respectively, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, and measuring; the determination method disclosed by the invention is convenient, accurate and reliable, and the content of the ethyl (2E, 4E)-ethyl-4-(pyridine-2-yl imino)-2-butenoate in the product can be really reflected.