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Method for testing content of sugar in fermentation liquor and application of method

A technology for fermentation broth and sugar content, applied in the field of biochemistry, can solve the problems of lack of large-scale instruments and equipment, personnel training gap, etc., and achieve the effects of low measurement cost, good reproducibility, and low operation requirements

Inactive Publication Date: 2017-05-31
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some small and medium-sized fermentation enterprises often do not have large-scale instruments and equipment due to limited strength, and there is also a certain gap in personnel training.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Follow the steps below to determine the sugar content in Streptomyces fermentation broth

[0052] 1. Take 50ml of fermentation broth from the sampling port of the fermenter;

[0053] 2. Take 1ml of fermentation broth with a pipette, add 9ml of distilled water, and mix well;

[0054] 3. Centrifuge at a high speed of 3000 r / min for 3 minutes in a centrifuge, and adjust the pH value of the supernatant to 10 with sodium hydroxide.

[0055] 4. Aspirate the supernatant, filter it through a 0.2μm filter membrane and set aside;

[0056] 5. Insert the glucose test strip into the glucose meter;

[0057] After the instrument shows that it can be measured, immerse the tip of the glucose test paper into the fermentation broth filtered through a 0.2μm filter membrane, pull it out quickly and wait for the reading result;

[0058] 6. Follow the formula to convert the glucose content into a value in grams per liter.

[0059] Glucose content in fermentation broth (g / L) = instrument rea...

Embodiment 2

[0061] Follow the steps below to determine the sugar content in Bacillus fermentation broth

[0062] 1. Take 100ml fermentation broth from the sampling port of the fermenter;

[0063] 2. Take 1ml of fermentation broth with a pipette, add 4ml of distilled water to dilute, and centrifuge at a high speed of 5000r / min for 1.5min in a centrifuge;

[0064] 3. Aspirate the supernatant, filter it through a 0.2 μm filter membrane and set aside; use sodium carbonate to adjust the pH value of the supernatant to 3.

[0065] 4. Insert the glucose test strip into the glucose meter;

[0066] 5. After the instrument shows that it can be measured, immerse the tip of the glucose test paper into the fermentation broth filtered by a 0.2μm filter membrane, pull it out quickly and wait for the reading result;

[0067] 6. Follow the formula to convert the glucose content into a value in grams per liter.

[0068] Glucose content in fermentation broth (g / L) = instrument reading × 0.18 × 5

Embodiment 3

[0070] Follow the steps below to determine the sugar content in E. coli fermentation broth

[0071] 1. Take 70ml of fermentation broth from the sampling port of the fermenter;

[0072] 2. Take 1ml of fermentation broth with a pipette, add 14ml of distilled water to dilute, and centrifuge at 4000r / min for 2min in a centrifuge;

[0073] 3. Aspirate the supernatant, filter it through a 0.2 μm filter membrane and set aside; use potassium carbonate to adjust the pH value of the supernatant to 7.3.

[0074] 4. Insert the glucose test strip into the glucose meter;

[0075] 5. After the instrument shows that it can be measured, immerse the tip of the glucose test paper into the fermentation broth filtered by a 0.2μm filter membrane, pull it out quickly and wait for the reading result;

[0076] 6. Follow the formula to convert the glucose content into a value in grams per liter.

[0077] Glucose content in fermentation broth (g / L) = instrument reading × 0.18 × 15

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Abstract

The invention provides a method for testing the content of sugar in fermentation liquor and application. The method comprises the following steps: performing centrifugal treatment on the fermentation liquor, diluting supernatant, adjusting the pH value, and testing the content of the sugar of the diluent by using a glucometer. The invention further provides the application of the method in testing the glucose content of the fermentation liquor, and preferably, the fermentation liquor includes fermentation liquor and microorganism fermentation liquor.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for measuring the sugar content of fermentation broth and its application. Background technique [0002] Glucose is widely used as a carbon source in the fermentation industry. Because it is cheap and easy to be used by microorganisms, it has become the most important carbon source in the fermentation industry. In the fermentation process, it is often necessary to strictly control the glucose content in order to achieve the purpose of increasing the yield of the fermentation target product and reducing the fermentation cost. For this reason, it is often necessary to measure the glucose concentration in the fermentation broth in time during the fermentation process and to use this as a basis for timely regulation of the glucose concentration in the fermenter. The most widely used glucose determination method is Fehling's reagent method. This method requires frequent preparat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N1/28G01N1/38
CPCG01N1/28G01N1/38G01N33/00G01N2001/386
Inventor 黄永春宋晓龙刘仲齐黄益宗张长波
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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