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Method for detecting free fatty acid content in compound traditional Chinese medicine

A technology of free fatty acids and compound traditional Chinese medicine, applied in the direction of measuring devices, material separation, instruments, etc., can solve the problems of determination uncertainty, narrow application range, heavy workload, etc., and achieve accurate detection results, wide linear range, and application range wide effect

Active Publication Date: 2013-04-03
SHINEWAY PHARMA GRP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free fatty acids are not suitable for determination by gas chromatography due to their high boiling points
In addition, the esterification derivatization treatment requires sample pretreatment, and different samples need to investigate many conditions of the derivatization reaction to ensure that the derivatization reaction occurs completely and quantitatively, but there is a certain degree of uncertainty in the determination of the final conditions
Therefore, the existing method has a long detection period, a large workload, low work efficiency, and a narrow scope of application; moreover, if the sample to be detected contains both free fatty acid and its methyl ester, the existing method can only measure the detected fatty acid. The total amount of free fatty acids and their methyl esters in the sample, but the content of free fatty acids cannot be determined separately, so the precision of the quantitative results is relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Content detection of linoleic acid in embodiment 1 Xinnaoqing Soft Capsules

[0023] Experimental Reagents and Instruments

[0024] Reagent: Xinnaoqing Soft Capsule (refined safflower oil, borneol, vitamin E, vitamin B6.), commercially available, produced by Shineway Pharmaceutical Co., Ltd.; linoleic acid reference substance purchased from SIGMA Company; acetonitrile (chromatographically pure, Fisher Company of the United States), methanol (chromatographically pure, Fisher Company of the United States).

[0025] Instrument: high performance liquid chromatography (Agilent, USA, type 1200, UV-detector).

[0026] Chromatographic conditions: chromatographic column AgilentXDB ODS column (4.6×250mm, 5μm); mobile phase acetonitrile-water (volume ratio 75:25); detection wavelength 203nm; flow rate 1ml / min; column temperature 35°C.

[0027] 1, Reference substance solution preparation

[0028] Weigh 180mg of linoleic acid reference substance, weigh it accurately, dilute to...

Embodiment 2

[0043]Content detection of linoleic acid in embodiment 2 Xinnaoqing Soft Capsules

[0044] Experimental Reagents and Instruments

[0045] Reagents: Xinnaoqing Soft Capsule (commercially available, produced by Shineway Pharmaceutical Co., Ltd.); linoleic acid reference substance purchased from SIGMA Company; acetonitrile (chromatographically pure, Fisher, USA), methanol (chromatographically pure, Fisher, USA).

[0046] Instrument: high performance liquid chromatography (Agilent, USA, type 1200, UV-detector).

[0047] Chromatographic conditions: chromatographic column AgilentXDB ODS column (4.6×250mm, 5μm); mobile phase acetonitrile-water (volume ratio 75:25); detection wavelength 203nm; flow rate 1ml / min; column temperature 35°C.

[0048] 1. Preparation of reference solution

[0049] Weigh 180mg of linoleic acid reference substance, weigh it accurately, dilute to 20ml with methanol, accurately draw 1ml, dilute to 50ml with methanol, and prepare 0.18mg / ml linoleic acid refe...

Embodiment 3

[0056] Content detection of linoleic acid in embodiment 3 Xinnaoqing Soft Capsules

[0057] Experimental Reagents and Instruments

[0058] Reagents: Xinnaoqing Soft Capsule (commercially available, produced by Shineway Pharmaceutical Co., Ltd.); linoleic acid reference substance purchased from SIGMA Company; acetonitrile (chromatographically pure, Fisher, USA), methanol (chromatographically pure, Fisher, USA).

[0059] Instrument: high performance liquid chromatography (Agilent, USA, type 1200, UV-detector).

[0060] Chromatographic conditions: chromatographic column AgilentXDB ODS column (4.6×250mm, 5μm); mobile phase acetonitrile-water (volume ratio 75:25); detection wavelength 203nm; flow rate 1ml / min; column temperature 35°C.

[0061] 1. Preparation of reference solution

[0062] Weigh 180mg of linoleic acid reference substance, weigh it accurately, dilute to 20ml with methanol, accurately draw 1ml, dilute to 50ml with methanol, and prepare 0.18mg / ml linoleic acid ref...

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Abstract

The invention discloses a method for detecting the free fatty acid content in a compound traditional Chinese medicine. By the method, the free fatty acid content in the compound traditional Chinese medicine is measured by high performance liquid chromatography; and a chromatographic signal is detected by an ultraviolet detector. Furthermore, the invention relates to a quality detection method for any one or more than one of linoleic acid, linolenic acid, palmitoleic acid, oleic acid, palmitic acid, stearic acid and palmitic acid. The method has the characteristics of high stability, high precision, wide linear range, wide application range and the like.

Description

technical field [0001] The invention relates to a method for detecting the quality of active ingredients in compound traditional Chinese medicines, in particular to a method for detecting the quality of free fatty acids in compound traditional Chinese medicines. technical background [0002] Free fatty acids are important active ingredients in many existing traditional Chinese medicine compound preparations. For example, oleic acid in Xinnaoqing soft capsule, linoleic acid, oleic acid, palmitic acid and stearic acid in Baiduoning injection are all active ingredients in traditional Chinese medicine preparations. Establishing a quality detection method for free fatty acids in compound traditional Chinese medicine is one of the important means to ensure reliable and stable efficacy of medicines. However, due to the high melting point of free fatty acids, the long carbon chain of fat solubility and the hydrophilic hydroxyl group, there is no absorption at other ultraviolet wave...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 刘军锋高钧韩红梅姜海胡雪娇
Owner SHINEWAY PHARMA GRP LTD
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