39 results about "Furaltadone" patented technology

The invention relates to an immune nano gold test strip for quickly detecting four nitrofuran medicaments. The immune nano gold test strip is prepared by the following steps of: synthesizing general hapten, immunogen and captured antigen aiming at the four nitrofuran medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone by using 5-nitrofurfural as a raw material, immunizing a domestic rabbit serving as an experimental animal to acquire polyclonal IgG antibody, preparing gold labeled antibody by using nano gold and the polyclonal IgG antibody, coating the gold labeled antibody solution on a Glass33 glass cellulose membrane, namely a combined release pad, coating the captured antigen and goat anti-rabbit antibody on a detection line and a quality control line of an AE99 nitrocellulose membrane respectively, sequentially sticking the AE99 nitrocellulose membrane, the combined release pad, namely the Glass33 glass cellulose membrane, an absorption pad Cotton linters 2668 and a sample pad Glass33 on a polyvinyl chloride (PVC) back board, and finally, cutting the back board into 4*50mm test strips. The test strip can simultaneously and quickly detect the four medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone in feed; and the cost of the test strip is less than 5 yuan, so the test strip is suitable to be widely applied in feed detection or food animal detection.

The invention relates to a furaltadone metabolite detection kit and provides an elisa kit for detecting furaltadone metabolite, comprising an enzyme yoke plate with an encrusting furaltadone metabolite derivate antigen, an enzyme yoke plate with an enzyme-labeling furaltadone metabolite derivate specific antibody or an encrusting furaltadone metabolite derivate specific antibody, and an enzyme-labeling furaltadone metabolite derivate antigen, wherein the furaltadone metabolite derivate antigen is obtained by coupling a furaltadone metabolite derivate hapten and carrier protein by an active ester method. The kit is convenient to use and has favorable performance in aspects, such as accuracy, sensitivity, specificity, and the like.

The invention relates to a label-free impedance type immunosensor for furaltadone residues, a preparation method and application thereof. The label-free impedance type immunosensor is prepared by 5 steps of preparing gold glue, preprocessing gold electrodes, embellishing the gold electrodes by utilizing 1,4-benzodiazepines mercaptan, self-assembling single-layer nanogold glue and fixing an antibody. The label-free impedance type immunosensor is characterized in that nanogold is fixed on the surface of the gold electrodes through a self-assembling method and used for fixing a monoclonal antibody of the furaltadone residues; the relation of the relative change rate %delta Rct and the concentration of AMOZ of impedance is firstly established when the label-free impedance type immunosensor isused; and the concentration of the AMOZ can be determined according to the relative change rate %delta Rct of the impedance of a sample to be detected. The label-free impedance type immunosensor andthe detection method have the characteristic rapid, simple and convenient operation and solve the defect of time waste in the process of detecting traditional furaltadone residues.

The invention discloses a furaltadone metabolite (AMOZ) derivatization hapten, a preparation method of an artificial antigen and an application of the artificial antigen. An active arm introduced by the hapten completely reserves feature structures of AMOZ derivatives; the electron cloud density is not influenced; active groups capable of being coupled to carrier protein are provided; the reaction condition is mild and the operation step is simple; and the prepared artificial antigen can be specifically combined with an antibody, has the characteristics of high potency, high sensitivity, high accuracy and the like, and can quickly detect residues of AMOZ in various products.

The invention relates to a furaltadone metabolite detection kit for detecting furaltadone metabolites, which comprises an enzyme-linked plate which is coated with furaltadone metabolite derivant antigen and an enzyme label furaltadone metabolite specific antibody or an enzyme-linked plate which is coated with furaltadone metabolite derivant specific antibody and an enzyme label furaltadone metabolite antigen, wherein the furaltadone metabolite derivant antigen is got through coupling furaltadone metabolite derivant hapten and carrier protein through adopting an active ester method. The invention has the advantages of excellent property in the aspects of accuracy, sensitivity, specificity and other aspects.

The invention provides a nitrofurans drug metabolite assay kit, which consists of a furazolidone metabolite test paper strip, a furaltadone metabolite test paper strip, a nitrofurazone metabolite test paper strip and a furantoin metabolite test paper strip. Each test paper strip consists of a sample pad, a colloidal gold pad, a nitrocellulose membrane, a sample adsorbing pad and a PVC (polyvinyl chloride) supporting plate, wherein the colloidal gold layer is a nitrofurans drug metabolite monoclonal antibody polyester film marked with colloidal gold, the nitrocellulose membrane is sequentially coated with nitrofurans drug metabolite coupled carrier protein as a test line (a T-line) and goat anti-mouse IgG antibody as a quality control line (a C-line). The nitrofurans drug metabolite assay kit is produced according to the nano colloidal gold technology, antigen-antibody specificity reactions and principles of immunity competitive inhibition reaction, and is used for testing whether samples contain nitrofurans drug metabolites or not.

The invention discloses a chemiluminescence detection kit (CLEIA) for a furaltadone metabolite (AMOZ). The kit comprises a kit body, a chemiluminescence plate disposed in the kit body and reagents disposed in the kit body. Apertures of the chemiluminescence plate are coated with an AMOZ coupling antigen. The reagents comprise enzyme labeled antibody concentrate, enzyme labeled antibody diluent, AMOZ series standard substance solution, chemiluminescence A liquid, chemiluminescence B liquid, concentrated washing liquid, concentrated redissolution liquid, and a derivatization agent. The detection kit has characteristics of high sensitivity, simpleness, rapidness and high accuracy. Compared with a traditional ELISA method, the operation time of the detection kit provide by the invention is largely reduced. The detection kit can be used for tissue (muscle and liver) detection of livestock and poultry, and used for AMOZ residue detection in aquatic products.

The invention relates to a furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit comprises a luminescence test plate, a furaltadone metabolite standard solution, a furaltadone metabolite monoclonal antibody working solution, an enzyme-labeled secondary antibody, a luminescence liquid, a sample diluent, and a washing concentrate. The furaltadone metabolite monoclonal antibody working solution is a solution of Balb-c mouse immune antibody, and the solution is diluted according to a volume ratio of 1:3500 for application, wherein a furaltadone metabolite-bovine serum albumin conjugate is taken as an immunogen of Balb-c mouse immune antibody; the enzyme-labeled secondary antibody is a goat anti mouse IgG working solution labeled by the furaltadone metabolite-horse radish peroxidase, and is diluted according to a volume ratio of 1:10000 for application; and the luminescence liquid comprises a luminescent substrate A, and a luminescent substrate B. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit is high in sensitivity, wide in linear dynamic range, long in optical signal duration, rapid in analyzing, stable in results, less in error, high in safety, and long in usage period.

The present invention provides an enzyme-linked immunoassay kit and a method for furaltadone metabolite detection. The enzyme-linked immunoassay kit comprises an enzyme label plate coated with coating antigen, specific furaltadone metabolite antibody, an enzyme marker, furaltadone metabolite standard solutions, a substrate coloration solution, a termination solution, a concentration washing solution, a concentration reconstituted solution, and 2-nitrobenzaldehyde. The present invention further provides a furaltadone metabolite enzyme-linked immunoassay kit detection method, which mainly comprises: carrying out a sample pretreatment, adopting the kit to detect, and finally analyzing a detection result. With the detection kit, furaltadone metabolite drugs in animal tissues, aquatic products and honey can be rapidly detected, characteristics of simple and rapid operation, high accuracy, high sensitivity, low cost and the like are provided, and the kit and the method are applicable for screening and on-site monitoring of a large number of samples.

The invention belongs to the field of organic synthesis, and in particular relates to a process for manufacturing furaltadone hydrochloride. The technical proposal comprises the following step a condensation reaction of 5-nitro frfural diacetate and morpholinomethylene amino oxazolone is performed under the hydrochloric acid condition to generate the furaltadone hydrochloride, wherein the weight ratio of the 5-nitro frfural diacetate to the morpholinomethylene amino oxazolone is 55:95. The process has the advantages that the process has high yield, and the reaction yield is between 85 and 90 percent (calculated the morpholinomethylene amino oxazolone); and the process simplifies the operation, and reduces a two-step reaction to a one-step reaction.

The invention discloses a kit for detecting furaltadone metabolites in a food. The kit comprises an ELISA plate coated with a furaltadone metabolite antigen, a furaltadone metabolite monoclonal antibody, a verdoperoxidase-labelled goat anti-mouse antiantibody, a standard solution, a substrate developing solution, stop buffer, concentrated scrubbing solution and concentrated reconstitution fluid. The furaltadone metabolite antigen is a conjugate of furaltadone metabolite half antigen and keyhole limpet haemocyanin; and the verdoperoxidase-labelled goat anti-mouse antiantibody is obtained by coupling marker enzyme verdoperoxidase and a goat anti-mouse antiantibody through a glutaraldehyde crosslinking method. The kit is high in sensitivity and stability and suitable for aquatic products such as fishes and shrimps and animal products such as chicken and pork.

The invention provides an immunodetection method capable of detecting Furaltadone metabolite AMOZ, MG (malachite green) and LMG (leucomalachite green) simultaneously. An AMOZ hapten and an LMG hapten are synthesized scientifically and coupled with carrier proteins respectively, and artificial antigens are prepared. Hybridoma cell strains aiming at the two objects are prepared respectively from the AMOZ artificial antigen and the LMG artificial antigen, a hybrid-hybridoma cell strain capable of secreting an anti-AMOZ and anti-LMG bispecific antibody is prepared with a hybrid-hybridoma technique, a multi-residue immunodetection method is established successfully, simultaneous detection of AMOZ, MG and LMG is realized, the limit of detection of AMOZ is 0.1 ng/mL, and the IC50 is 1.3 ng/mL; the limit of detection of LMG is 4.8 ng/mL, and the IC50 is 45.3 ng/mL. The method has high specificity and higher sensitivity, has broad application prospect in multi-residue detection of AMOZ, MG and LMG in food and is simple and convenient to operate.

The invention relates to a colloidal gold test strip for detecting four kinds of nitrofuran metabolites and a preparation method thereof. The four kinds of nitrofuran comprise: furazolidone (AOZ), furaltadone (AMOZ), nitrofurantoin (AHD), and furacilin (SEM). The invention aims at providing the colloidal gold test strip for simultaneously detecting residues of four kinds of nitrofuran metabolites with fast and accurate characteristics and simple operation and the preparation method thereof. The aspects are improved, such as a method for marking four kinds of antibodies with colloidal gold, lineation on a nitrocellulose membrane (NC membrane), and treatment fluid for a combination pad; results show that the test strip has the advantages of high integration, high specificity, good repeatability, low cost, and the like. The operation is simple, the result is accurate, the method can be used for producing the test strip for combined detection of four kinds of nitrofuran metabolites, and the method has a certain guidance effect for producing test strips for combined detection.

The invention discloses a stable-isotope-labeled furaltadone metabolite and a synthesis method thereof. The method includes reacting stable-isotope-labeled epichlorohydrin which is used as a stable isotope labeling source and morpholine to obtain stable-isotope-labeled morpholinyl epoxypropane; then reacting with hydrazine hydrate to obtain stable-isotope-labeled 1-hydrazino-3-morpholinyl-2-propanol; and finally reacting with substituted formate under an alkaline condition to obtain the stable-isotope-labeled furaltadone metabolite. The invention provides the synthesis method of the stable-isotope-labeled furaltadone metabolite for the first time; the stable isotope labeling raw material is cheap and easy to obtain, the synthesis process is simple, the product is easy to separate and purify, and the chemical purity and isotope abundance of the obtained product both reach 99% or above, meeting the requirement for serving as a standard reagent for quantitatively detecting furaltadone metabolite. The metabolite has high application value and good economy performance.

The invention belongs to the field of veterinary drug residue analysis, and particularly relates to a novel chemical synthesis method of 5- methylmorpholine-3-amino-2-oxazolidinyl ketone, which is used in veterinary drug residue analysis and is a marker for furaltadone residue. The synthesis method is characterized in that hydrazine hydrate and morpholine epoxy propane are used as raw materials and react with each other under alkali condition to generate intermediate 1-diazanyl-3-morpholine-propanol; the product then reacts with substituted formate under alkali condition to generate the end product 5- methylmorpholine-3-amino-2-oxazolidinyl ketone. The invention provides for the first time the complete synthesis method of 5- methylmorpholine-3-amino-2-oxazolidinyl ketone (AMOZ), the synthesis procedure is simple and operational, and the reaction condition is mild.

The invention relates to an aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application, the kit comprises an immunochromatography test strip and an aggregation-induced emission fluorescent probe, the immunochromatography test strip comprises a polyvinyl chloride base plate, the upper surface of the polyvinyl chloride base plate is provided with a nitrocellulose membrane, a water absorption pad and a sample pad, and the nitrocellulose membrane is arranged on the upper surface of the polyvinyl chloride base plate. A detection line T1, a detection line T2 and a quality control line C are sequentially arranged on one side, close to the sample pad, of the upper surface of the nitrocellulose membrane; the aggregation-induced emission fluorescent probe comprises a green aggregation-induced emission fluorescent microsphere probe prepared from BTPETD and a red aggregation-induced emission fluorescent microsphere probe prepared from TPB. The device has the advantages of convenience in use and simplicity in operation. The method is used for qualitative and quantitative detection of the furaltadone metabolite and the nitrofurantoin metabolite at the same time, the detection efficiency is greatly improved, and the detection sensitivity can be effectively improved.