A method for simultaneous determination of 31 components in compound salvia miltiorrhiza extract or related medicinal materials

A technology for compound salvia miltiorrhiza and extracts, applied in material separation, measuring device, analyzing materials, etc., can solve problems such as inconvenient mass balance, and achieve the effects of saving time and cost, large measuring range, and comprehensive preparation quality.

Active Publication Date: 2022-03-08
TIANJIN TASLY PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application of multiple analytical methods has brought great inconvenience to the study of mass balance

Method used

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  • A method for simultaneous determination of 31 components in compound salvia miltiorrhiza extract or related medicinal materials
  • A method for simultaneous determination of 31 components in compound salvia miltiorrhiza extract or related medicinal materials
  • A method for simultaneous determination of 31 components in compound salvia miltiorrhiza extract or related medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: detection method

[0088] 1. Preparation of standard solution and internal standard solution

[0089] 1.1 Preparation of standard series solutions

[0090] Weigh an appropriate amount of the standard substance to be tested, weigh it accurately, add 70% methanol water to make a solution with a concentration of 500 μg / mL, and use it as the stock solution of each sample to be tested. Accurately measure the appropriate amount of each stock solution of the analyte, and use 70% methanol water to prepare tetrasaccharide (concentration is about 70000ng / mL), sucrose (concentration is about 50000ng / mL), salvianolic acid B (concentration is about 12000ng / mL) mL), ginsenoside Rg 1 (concentration is about 8000ng / mL), glucose (concentration is about 6000ng / mL), trisaccharide, ginsenoside Rb 1 , Ginsenoside Rd (concentration is about 4000ng / mL), pyroglutamic acid, rosmarinic acid, notoginsenoside R 1 (concentration of about 2000ng / mL) and the rest of the analyte (con...

Embodiment 2

[0105] The assay of embodiment 2 31 kinds of compositions

[0106] 1. Preparation of sample solution for test

[0107] Compound Danshen extracts S01-S05 (batch numbers 20131205, 20141103, 20120201, 20150310 and 20150310) were provided by Tasly Pharmaceutical Group Co., Ltd.; Danshen medicinal materials S06-S27 were collected from several provinces in China, and Panax notoginseng medicinal materials S28-S52 were collected from Yunnan Province, and crushed at the Tasly Research Institute.

[0108] Take 0.10 g of compound Danshen extract, Danshen extract, Panax notoginseng extract, Danshen powder and Panax notoginseng powder, weigh them accurately, add 10 mL of 70% methanol, sonicate for 30 min, centrifuge for 10 min (16,000 rpm), filter through 0.22 μm Membrane, respectively accurately measure the same amount of sample solution and IS reference solution, and mix them to prepare the sample solution containing internal standard for RP-HILIC-MS / MS analysis. All solutions were sto...

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Abstract

The present invention relates to a method for simultaneously measuring 31 components in a compound salvia miltiorrhiza extract or related medicinal materials. The method adopts a direct series-reversed-phase-hydrophilic interaction chromatography-mass spectrometry method, and the method comprises the following steps: 1 Preparation of sample solution; Step 2 Preparation of standard series solution; Step 3 Preparation of internal standard solution; Step 4 Preparation of test solution; Step 5 Take the solution obtained in Step 4 and inject it into direct series reversed-phase-hydrophilic interaction liquid chromatography The mass spectrometry system obtains the extracted ion chromatograms of 31 analytes, and calculates the content of 31 components in the compound salvia miltiorrhiza extract or related medicinal materials according to the extracted ion chromatograms.

Description

Technical field: [0001] The invention relates to a method for detecting effective components of traditional Chinese medicine, in particular to a method for simultaneously determining 31 components in compound salvia miltiorrhiza extract or related medicinal materials. Background technique: [0002] The study of mass balance is of great significance for elucidating the composition of the chemical substance groups of traditional Chinese medicines. The chemical composition of traditional Chinese medicine is extremely complex, containing many primary metabolites and secondary metabolites. [0003] Preliminary studies have shown that compound Danshen dripping pills (made from Danshen, Panax notoginseng, and borneol, see the 2015 edition of the Chinese Pharmacopoeia for specific preparation methods) mainly contain oligosaccharides, amino acids, nucleosides, organic acids, phenolic acids, saponins, and tanshinones compound. Due to the large differences in the structure and physic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/89
CPCG01N30/02G01N30/89
Inventor 佟玲李云飞李东翔孙万阳芦勤玮
Owner TIANJIN TASLY PHARMA CO LTD
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