4 results about "Cefepime" patented technology

The invention provides a fluorenyl alcohol derivative which can be used as an antibiotic adjuvant with a broad-spectrum synergistic effect. According to the compound, bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are taken as targets, and a series of fluorenyl alcohol derivatives targeting the bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are obtained through screening. The preferable compound WTU-15 can improve the antibacterial activity of antibiotics such as polymyxin, cefepime, tetracycline and the like on negative bacteria such as sensitive and drug-resistant escherichia coli, salmonella, klebsiella pneumoniae, acinetobacter baumannii and the like, and can also improve the in-vivo treatment effect of cefepime on mice infected by the drug-resistant acinetobacter baumannii. And the visceral organs of the mice survived after administration have fewer bacteria, and the mice are better after being healed. Normal physiological and visceral organ functions of the mouse are not affected by single and continuous administration of the WTU-15.

The application discloses a preparation method of high-purity cephalosporin bipu ester side chain (1a), and a reaction route is shown as follows. The method is characterized in that 2a is used as a starting material to react with triphosgene to generate compound 3a, and then the compound 3a is used to react with p-nitrophenol to generate compound 1a. The product has high purity and high reaction yield, and is suitable for industrial production.

This invention discloses a method for simultaneously detecting cefepime and its metabolites, as well as prednisolone residues in milk. The method includes the following steps: taking a milk sample, extracting with acetonitrile, centrifuging, and collecting the supernatant; adding acetonitrile-saturated n-hexane for liquid-liquid extraction to remove fat; concentrating the extracted solution, reconstituted with phosphate buffer at pH 8.5 ± 0.2, purifying with a hydrophilic-lipophilic equilibrium solid-phase extraction column, eluting, concentrating, and reconstituted to obtain the test solution; and detecting the test solution using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). This invention, through optimized pretreatment processes, particularly the dual purification strategy of "n-hexane degreasing + HLB solid-phase extraction" combined with precise pH adjustment, effectively removes complex fat and protein matrix interferences in milk, significantly reducing matrix effects; combined with optimized gradient elution procedures and multiple reaction monitoring (MRM) mass spectrometry conditions, it achieves simultaneous, rapid, and highly sensitive detection of cefepime, deacetylated cefepime, and prednisolone.

The application discloses a detection kit for simultaneously determining the concentrations of 12 kinds of antibiotic drugs in trace serum and application, the 12 kinds of antibiotics including vancomycin, meropenem, imipenem, cilastatin, trimethoprim, linezolid, voriconazole, piperacillin, cefoperazone, sulfamethoxazole, cefepime and ceftazidime; the detection kit comprises pretreatment reagents and a liquid chromatography mobile phase; the pretreatment reagents comprise a protein precipitant and purified water; the protein precipitant comprises 50v / v% of methanol and 50v / v% of acetonitrile; the liquid chromatography mobile phase comprises phase A and phase B, the phase A comprises 0.02% of formic acid and 99.98% of water, and the phase B comprises 100% of acetonitrile. The method of the application only needs 10 muL of human serum sample, and can complete the detection within 4 min, can quantitatively determine 12 kinds of antibiotics with significant structural differences, and is simple, rapid, sensitive, accurate and specific.