38 results about "Cefquinome" patented technology

A novel 4-halo-2-oxyimino-3-oxo butyric acid-N,N-dimethyl formiminium chloride chlorosulfate of formula (I) useful in the preparation of cephalosporin antibioticswhereinX is chlorine or bromine; R is hydrogen, C1-4 alkyl group, an easily removable hydroxyl protective group, —CH2COOR5, or —C(CH3)2COOR5, wherein R5 is hydrogen or an easily hydrolysable ester group. The compound of formula (I) is prepared by reacting 4-halo-2-oxyimino-3-oxobutyric acid of formula (IV1),wherein X, R and R5 are as defined above, with N,N-dimethylformiminium chloride chlorosulphate of formula (VII)in an organic solvent at a temperature ranging from −30° C. to −15° C. The cephalosporins that may be prepared from the intermediate include cefdinir, cefditoren pivoxil, cefepime, cefetamet pivoxil, cefixime, cefmenoxime, cefodizime, cefoselis, cefotaxime, cefpirome, cefpodoxime proxetil, cefquinome, ceftazidime, cefteram pivoxil, ceftiofur, ceftizoxime, ceftriaxone and cefuzonam.

Cefquinome liposome, which belongs to the field of pharmaceutics, has a particle size of less than 1000 nm, and is mainly prepared from the following raw materials by weight: 1 part of cefquinome, 1-40 parts of phospholipid, 0-15 parts of sterol or soy isoflavone glucoside or soyasapogenol, and 0-15 parts of additives. The cefquinome liposome can be prepared into a liquid preparation, and can also be prepared into a solid preparation by adding a proper amount of a support agent. The cefquinome liposome or cefquinome long circulating liposome prepared in the invention contains no irritant substances, and can relieve anaphylactic reaction; the obtained preparation has good stability, has an average liposome particle size of less than 1000 nm, and has encapsulation efficiency of more than 80%. The preparation method is mature in process, simple, practical, and suitable for industrial production.

The invention belongs to the field of medicine, and relates to a new dosage form of veterinary medicine, in particular to a cefquinome sulfate lung-targeting liposome preparation and a preparation method thereof. The cefquinome sulfate lung-targeting liposome preparation provided by the invention has the advantages of good targeting, stable quality, high encapsulation efficiency and the like. The cefquinome sulfate lung-targeted liposome provided by the present invention is easy to use, has active and passive targeting effects, and the drug concentrates in the target organ after injection to take effect, reduces the loss of the drug, improves the curative effect, and reduces the effect on the Toxic side effects in other organs.

The invention discloses a method for improving the detection recovery rate of cefquinome residue in animal-derived food and reducing a substrate effect. According to the method, moisture is effectively removed through freeze drying; the recovery rate of the cefquinome residue in the animal-derived food is obviously improved; the substrate effect is reduced, so that a detection result is more accurate; the reasonable clinical application of cefquinome in veterinary medicine can be more effectively monitored; the safety of the animal-derived food for people is guaranteed.

Cefquinome powder injection is characterized by comprising crystalline cefquinome, and polyethylene glycol and amino acid surfactant acting as crystal transition inhibitory auxiliaries; the mass ratioof the polyethylene glycol to the amino acid surfactant is 10:(0.5-2). A preparation method of the cefquinome powder injection includes the steps of (1) melting, to be specific, heating the polyethylene glycol to 50-65 DEG C for melting, adding the crystalline cefquinome and the amino acid surfactant, and mixing to obtain melt A; (2) cooling, to be specific, quickly cooling the melt A to solid state to obtain solid mixture B; (3) crushing the solid mixture B.

The invention discloses a compound medicament. The compound medicament comprises a main drug, which is cefquinome or salt thereof and tedizolid or phosphate thereof. The compound medicament is a breast injectant in dosage form and is used for treating mastitis of cows in the lactation period. The invention further discloses a preparation method of the compound medicament, the method comprises thefollowing steps: mixing the main drug, a stabilizing agent and a solvent uniformly, and enabling the main drug and an ingredient to be dissolved to obtain a settled solution; enabling an antioxidant,a synergist and a suspending agent to be mixed with the settled solution, and stirring uniformly; afterwards, grinding for 20-50 min by using a colloid mill to obtain a finished product. The compoundmedicament realizes a synergistic anti-microbial effect through two kinds of mechanisms in which the cefquinome or the salt thereof and the tedizolid or the phosphate thereof are jointly combined, thesynthesis of a cell wall is jointly inhibited and the synthesis of protein is inhibited. The compound medicament further has the advantages of good fluidity, good drug release performance and convenient drug administration.

The invention discloses a composite cefquinome microsphere gel preparation and a preparation method thereof, and belongs to the technical field of medicines. The composite cefquinome microsphere gel preparation for veterinary use is prepared by adopting a spray drying method to prepare microspheres, adopting cefquinome as the medicine for the microspheres, adopting polylactic acid / polylactic acid-glycolic acid as a coating carrier material, and adopting hyaluronic acid and water as gel bases to prepare the composite cefquinome microsphere gel preparation. The microspheres are of 5-30umd in grain size, which is beneficial to lung targeting of the main medicine cefquinome, the cefquinome can easily gather at a target organ, blood clearance is reduced, long-time circulation is benefited, and pharmacological efficacy is improved. In addition, by the aid of the gel bases, contact of the medicine and interstitial fluid is reduced, the time of the medicine flowing into a bloodstream is prolonged, and a slow-release effect of the preparation is further increased.

The invention discloses a specific anti-ceftiofur monoclonal antibody hybridoma cell strain 2E5 and the application thereof and belongs to the technical field of food safety immunodetection. The monoclonal cell strain 2E5 is preserved in the China General Microbiological Culture Collection Center, CGMCC for short, with the preservation number of CGMCC No.10873. The crude drug ceftiofur and BSA are coupled to prepare immunogen by means of the active ester method, and ceftiofur and OVA are coupled to prepare coating antigen. The specific anti-ceftiofur hybridoma cell strain is obtained after a mouse is immunized. The monoclonal antibody secreted by the cell strain only aims at ceftiofur specifically, the crossing-over rates between the monoclonal antibody and cefalexin and between the monoclonal antibody and cefquinome are 3.12% and 5.3% respectively, the crossing-over rates between the monoclonal antibody and other cephalosporin antibiotics are all smaller than 0.1%, and therefore specificity is high. The anti-ceftiofur monoclonal antibody hybridoma cell strain has high detection sensitivity and compatibility, the crossing-over rates with other cephalosporin antibiotics are low, and therefore specific detection of ceftiofur can be achieved. The invention also provides a new idea for preparing a specific antibody aiming at a specific cephalosporin so as to obtain a good specific monoclonal cell strain.

The invention belongs to the technical field of analytical chemistry and veterinary drug residue analysis, and particularly relates to an electrochemical receptor sensor for detecting beta-lactam antibiotics, and a preparation method and application thereof. The electrochemical receptor sensor comprises an electrochemical probe, a signal probe and a novel composite nano material, the electrochemical probe is thionine, the signal probe is HRP labeled ampicillin (HRP-AMP), and the composite nano material is graphene / thionine / glassy carbon electrode (GO / TH / GCE). The method disclosed by the invention can be used for detecting cephalexin, cefquinome, ceftiofur, penicillin G and ampicillin, thereby providing a rapid and sensitive method for the detection of such substances in foods. The detection method established by the invention has high accuracy and good sensitivity, and has the advantages of saving a lot of time and cost compared with the existing method, and thus has a better market application value.