1results about How to "Short peak time" patented technology

The application belongs to the technical field of biomedicine, and specifically discloses an exonuclease Exo III mutant and related application thereof. Single-point or multi-point combined mutations are introduced at the E71L, Q133I, R170W, D188A and R209K sites of the wild-type sequence shown in SEQ ID NO:1, so as to solve the problems of easy deactivation, high background signal and poor storage stability of the wild-type enzyme in isothermal amplification, thereby greatly improving amplification sensitivity and shortening peak time in DNA / RNA isothermal amplification detection such as RPA and RTMA, and endowing the reagent with excellent high-temperature resistance, so that the reagent can be widely applied to gene detection, pathogen screening, bedside detection, environmental monitoring and development of ambient storage type in-vitro diagnostic kits.