8results about How to "Short peak time" patented technology

The invention discloses a mobile phase suitable for simultaneous detection of ambroxol hydrochloride and / or clenbuterol hydrochloride by high performance liquid chromatography. The mobile phase is a mixed solution composed of sodium sulfosuccinate dioctyl ester methanol, water and acetic acid, wherein the mass fraction of the sodium sulfosuccinate dioctyl ester methanol is 0.3%-0.8%; the volume ratio of the sodium sulfosuccinate dioctyl ester methanol with the mass fraction of 0.3%-0.8% to the water to the acetic acid is (740-760): (240-260): 10; the pH value of the mixed solution is 2.8 to 3.2. According to the present invention, the mobile phase is combined with the high performance liquid chromatography to detect the ambroxol hydrochloride oral solution, such that the ambroxol hydrochloride and the clenbuterol hydrochloride can be simultaneously detected, the ambroxol hydrochloride and the clenbuterol hydrochloride can be effectively separated from other impurity peaks, the peak appearance time of the ambroxol hydrochloride can be significantly reduced, and the service life of the chromatographic column can be effectively prolonged.

The invention discloses a eutectic of omeprazole and 3, 5-dinitrobenzoic acid, the molar ratio of omeprazole to 3, 5-dinitrobenzoic acid is 1: 1, and compared with omeprazole, the eutectic has higher stability, in-vivo absorption speed and onset speed.

The invention discloses a method for separating and preparing megastigmatrienone isomers, and belongs to the technical field of isomer separation and preparation. The technical problem to be solved is that in the prior art, mg-level separation preparation of a megastigmatrienone single-configuration isomer cannot be rapidly realized. The key point of the technical scheme is as follows: the method comprises the following steps: (1) mixing megastigmatrienone with methanol to obtain a mixed solution; (2) separating the mixed solution by adopting a reversed-phase preparative chromatography to obtain an isomer of megastigmatrienone; wherein the chromatographic conditions in the reversed-phase preparative chromatography in the step (2) are as follows: a chromatographic column is a reversed-phase semi-preparative chromatographic column, the sample size is 100-800 [mu] L, the flow velocity is 0.1-0.8 mL / min, the detection wavelength is 308 nm, an eluent is methanol-THF-H2O in a ratio of 37.5: 7.5: 55, and isocratic elution is performed.

The invention provides a method and a kit for detecting blood concentration of compound sulfamethoxazole and metabolite thereof. According to the method, a methanol precipitator is combined with Oasis PRiME HLB reversed-phase solid-phase extraction, the centrifugal process of plasma treatment is reduced, traditional activation and balance steps are omitted, time is saved, meanwhile, the method is more environmentally friendly, and the extraction recovery rate of a to-be-detected substance can be improved; gradient elution is replaced by isocratic elution, the balance returning time is avoided, the overall operation time is shorter, the separation application is not influenced, the operation time is only 6 minutes, all the to-be-measured substances can be accurately and simultaneously measured only by 1 microliter, and the peak appearance time is 2 minutes. According to the invention, trimethoprim, sulfamethoxazole and metabolites thereof sulfamethoxazole-N-acetyl and 4-nitro sulfamethoxazole in human plasma can be monitored at the same time by using a treatment method and a computer method.

The application belongs to the technical field of biomedicine, and specifically discloses an exonuclease Exo III mutant and related application thereof. Single-point or multi-point combined mutations are introduced at the E71L, Q133I, R170W, D188A and R209K sites of the wild-type sequence shown in SEQ ID NO:1, so as to solve the problems of easy deactivation, high background signal and poor storage stability of the wild-type enzyme in isothermal amplification, thereby greatly improving amplification sensitivity and shortening peak time in DNA / RNA isothermal amplification detection such as RPA and RTMA, and endowing the reagent with excellent high-temperature resistance, so that the reagent can be widely applied to gene detection, pathogen screening, bedside detection, environmental monitoring and development of ambient storage type in-vitro diagnostic kits.

The invention discloses a liquid chromatography method for rapidly determining D / L-amygdalin in traditional Chinese medicine semen armeniacae amarae / semen persicae. A chromatographic column stationary phase used in the method is a silica gel matrix bonded biphenyl reversed-phase chromatography filler; wherein the type of the silica gel substrate comprises full-porous silica gel, core-shell surface porous silica gel and hybrid silica gel; the bonded chemical functional group is a biphenyl group. According to the method, silica gel substrate bonded biphenyl reversed-phase chromatographic packing is adopted as a chromatographic column stationary phase, and optimal chromatographic column temperature, mobile phase type, flow velocity, elution method and the like are combined, so that the D / L-amygdalin in the traditional Chinese medicine semen armeniacae amarae / semen persicae is rapidly measured; according to the present invention, with the method, the baseline separation of the D / L-amygdalin in the sample can be achieved within the short analysis time, the good separation selectivity on the two epimers of the D / L-amygdalin is provided, the universality is provided, and the method can be simultaneously suitable for the determination of the D / L-amygdalin in the traditional Chinese medicine semen armeniacae amarae and the semen persicae.

The present invention discloses a novel method for rapid separation of charged heterogeneous substances in protein products. The method uses isocratic elution ion exchange chromatography to separate charged heterogeneous substances in protein products, that is, simple and convenient isocratic elution is used to replace the commonly used gradient elution. The advantages are: significantly accelerating the separation speed and improving the analysis efficiency; simplifying the experimental process and reducing the manpower and material costs; and having wide applicability. The same method can be used for the separation and analysis of charged heterogeneous substances in a variety of similar protein products.

The invention discloses a method and a device for detecting pentachlorophenol and sodium salt thereof in air of a workplace, and belongs to the technical field of air analysis and detection. The method for detecting the pentachlorophenol and the sodium salt thereof in the air of the workplace comprises the following steps: S01, sampling the air; s02, carrying out derivatization pretreatment and extraction; s03, loading a sample, and carrying out GC-MS detection; s04, drawing a standard curve; and S05, calculating the content of pentachlorophenol and the sodium salt thereof in the air of the workplace according to the detection result obtained in the step S03 and the standard curve obtained in the step S04. According to the method for detecting the pentachlorophenol and the sodium salt of the pentachlorophenol in the air of the workplace, the quantitative concentration can reach 0.02 mg / m < 3 > (by collecting 15L of an air sample), and the lower quantitative concentration can be reached; the adding standard recovery rate is 96-98%, and the correlation coefficient in the range of 0-10mg / L is greater than or equal to 0.999. According to 0.3 mg / m < 3 > of pentachlorophenol and sodium salt thereof OELs (PC-TWA), the method can be used for detecting pentachlorophenol and sodium salt thereof at the concentration level above the contact grade I.