2results about How to "Short peak time" patented technology

The invention discloses a method for separating and preparing megastigmatrienone isomers, and belongs to the technical field of isomer separation and preparation. The technical problem to be solved is that in the prior art, mg-level separation preparation of a megastigmatrienone single-configuration isomer cannot be rapidly realized. The key point of the technical scheme is as follows: the method comprises the following steps: (1) mixing megastigmatrienone with methanol to obtain a mixed solution; (2) separating the mixed solution by adopting a reversed-phase preparative chromatography to obtain an isomer of megastigmatrienone; wherein the chromatographic conditions in the reversed-phase preparative chromatography in the step (2) are as follows: a chromatographic column is a reversed-phase semi-preparative chromatographic column, the sample size is 100-800 [mu] L, the flow velocity is 0.1-0.8 mL / min, the detection wavelength is 308 nm, an eluent is methanol-THF-H2O in a ratio of 37.5: 7.5: 55, and isocratic elution is performed.

The application belongs to the technical field of biomedicine, and specifically discloses an exonuclease Exo III mutant and related application thereof. Single-point or multi-point combined mutations are introduced at the E71L, Q133I, R170W, D188A and R209K sites of the wild-type sequence shown in SEQ ID NO:1, so as to solve the problems of easy deactivation, high background signal and poor storage stability of the wild-type enzyme in isothermal amplification, thereby greatly improving amplification sensitivity and shortening peak time in DNA / RNA isothermal amplification detection such as RPA and RTMA, and endowing the reagent with excellent high-temperature resistance, so that the reagent can be widely applied to gene detection, pathogen screening, bedside detection, environmental monitoring and development of ambient storage type in-vitro diagnostic kits.