Method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography

Abstract

The invention discloses a method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography. The method is characterized by comprising the following steps: (1) preparing floral leaf extraction solution: carrying out ultrasonic extraction on floral leaf powder by using a first extraction solvent, carrying out centrifugal separation, andfiltering to obtain floral leaf extracting solution; (2) preparing an extract sample: carrying out ultrasonic extraction on the floral leaf extracting solution by utilizing a second extracting solvent, and filtering to obtain an extract detection sample; (3) preparing a standard working solution: diluting a cannabinoid standard substance into the standard working solution by using methanol; and (4) high performance liquid chromatography determining: carrying out sample introduction detection on the extract detection sample and the standard working solution by using high performance liquid chromatography to obtain a standard curve, and calculating the cannabinoid component content of the extract detection sample according to the standard curve by using high performance liquid chromatography data processing software. The method has the advantages of short peak appearance time, short detection time, high detection efficiency and accurate and stable detection result, and is suitable for industrial detection.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural phytochemical components, and in particular relates to a method for detecting cannabinoids in industrial hemp flowers and leaves and extracts thereof by high performance liquid chromatography. Background technique [0002] Industrial hemp refers to hemp that has been legally grown and contains less than 0.3% tetrahydrocannabinol (THC). At present, it has been legally grown and processed in Yunnan and Heilongjiang. In 2019, industrial hemp has become the hottest investment field, and it is also an emerging hot topic in the big health industry. The flowers and leaves of industrial hemp contain cannabinoids and non-cannabinoids, which are widely used in the field of medicine. Among them, the cannabinoids are mainly divided into tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabidiol (CBG), cannabidiol (CBDV), cannabichromene (CBC) , THCV and dronabi...

AI Technical Summary

Problems solved by technology

Since the identification of industrial hemp depends on the content of tetrahydrocannabinol (THC) contained in hemp, other cannabinoids such as cannabidiol (CBD), cannabinol (THCV) and cannabidiol (CBDV) are in There are often overlapping phenomena in thin-layer chromatography and high-performance liquid chromatography. In addition, there are no standardized industry standards and related detection methods. There are technical barriers in the development of the industry, and most cannabinoids are mostly isomers with close molecular weights. , in the actual detection of cannabinoid content, when the retention time of two standard substances is consistent under certain conditions, there will be peak overlap, such as cannabidiolic acid (CBDA), hypohydrocannabinol (THCV), It is difficult to separate cannabidolol (CBG) and cannabinoidolic acid (CBGA) well, and they often appear at the same time at the same retention time, resulting in high purity but low content detection results. Process development has brought great trouble, and most of the current testing methods can only simply measure 1 to 4 kinds of cannabis components, and cannot accurately detect the content of multiple or full-spectrum cannabinoids

In addition, the existing literature and related patent methods have cumbersome detection operations, relatively long detection time, high requirements for operators, and cannot complete the content determination accurately and quickly

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