Method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography

A high-performance liquid chromatography and extraction technology, which is applied in the field of high-performance liquid chromatography detection of cannabinoids in industrial hemp flowers and leaves and their extracts, can solve the problem of inaccurate detection of cannabinoid content, high requirements for operators, and high content Low detection results and other problems, to achieve the effect of simple and convenient detection method, accurate detection results and short detection time

Inactive Publication Date: 2020-12-04
滇麻生物科技(曲靖)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the identification of industrial hemp depends on the content of tetrahydrocannabinol (THC) contained in hemp, other cannabinoids such as cannabidiol (CBD), cannabinol (THCV) and cannabidiol (CBDV) are in There are often overlapping phenomena in thin-layer chromatography and high-performance liquid chromatography. In addition, there are no standardized industry standards and related detection methods. There are technical barriers in the development of the industry, and most cannabinoids are mostly isomers with close molecular weights. , in the actual detection of cannabinoid content, when the retention time of two standard substances is consistent under certain conditions, there will be peak overlap, such as cannabidiolic acid (CBDA), hypohydrocannabinol (THCV), It is difficult to separate cannabidolol (CBG) and cannabinoidolic acid (CBGA) well, and they often appear at the same time at the same retention time, resulting in high purity but low content detection results. Process development has brought great trouble, and most of the current testing methods can only simply measure 1 to 4 kinds of cannabis components, and cannot accurately detect the content of multiple or full-spectrum cannabinoids
In addition, the existing literature and related patent methods have cumbersome detection operations, relatively long detection time, high requirements for operators, and cannot complete the content determination accurately and quickly

Method used

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  • Method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography
  • Method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography
  • Method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography

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Embodiment 1

[0031] The method for the high performance liquid chromatography detection industrial hemp mosaic leaf described in present embodiment 1, comprises the following steps:

[0032]①Preparation of flower leaf extract leaves: first place the cannabis flower leaves in an oven for baking, the baking conditions of the cannabis flower leaves are: continuous baking at 100°C for 1 hour, and then the roasted cannabis flower leaves Send it into the pulverizer and crush it into flower leaf powder. The crushed particle size of the flower leaf powder is 10 mesh. The industrial hemp flower leaves are fresh flower leaves, dried flower leaves and extracted flower leaf residues, and then accurately weigh the flower leaves The powder is placed in an Erlenmeyer flask with a ground mouth, and the first extraction solvent is added to the beaker. The first extraction solvent is analytically pure methanol, and the mass ratio of the first extraction solvent to the flower leaf powder is 1:90, and then the...

Embodiment 2

[0040] The method for the high performance liquid chromatography detection industrial hemp mosaic leaf described in present embodiment 2, comprises the following steps:

[0041] ①Preparation of flower leaf extract leaves: first place the cannabis flower leaves in an oven for baking, the baking conditions of the cannabis flower leaves are: continuous baking at 120°C for 2 hours, and then the roasted cannabis flower leaves Send it into the pulverizer and crush it into flower leaf powder. The crushed particle size of the flower leaf powder is 50 mesh. The industrial hemp flower leaves are fresh flower leaves, dried flower leaves and extracted flower leaf residues, and then accurately weigh the flower leaves The powder is placed in a Erlenmeyer flask with a ground mouth, and the first extraction solvent is added to the beaker. The first extraction solvent is analytical pure methanol, superior grade pure methanol or chromatographically pure methanol. The mass ratio of the first extr...

Embodiment 3

[0049] The method for the high performance liquid chromatography detection industrial hemp mosaic leaf described in present embodiment 3, comprises the following steps:

[0050] ①Preparation of flower leaf extract leaves: first place the cannabis flower leaves in an oven for baking, the baking conditions of the cannabis flower leaves are: continuous baking at 140°C for 3 hours, and then the roasted cannabis flower leaves Send it into the pulverizer and crush it into flower leaf powder. The crushed particle size of the flower leaf powder is 80 mesh. The industrial hemp flower leaves are fresh flower leaves, dried flower leaves and extracted flower leaf residues, and then accurately weigh the flower leaves The powder is placed in a Erlenmeyer flask with a ground mouth, and the first extraction solvent is added to the beaker. The first extraction solvent is analytical pure methanol, superior grade pure methanol or chromatographically pure methanol. The mass ratio of the first extr...

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Abstract

The invention discloses a method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography. The method is characterized by comprising the following steps: (1) preparing floral leaf extraction solution: carrying out ultrasonic extraction on floral leaf powder by using a first extraction solvent, carrying out centrifugal separation, andfiltering to obtain floral leaf extracting solution; (2) preparing an extract sample: carrying out ultrasonic extraction on the floral leaf extracting solution by utilizing a second extracting solvent, and filtering to obtain an extract detection sample; (3) preparing a standard working solution: diluting a cannabinoid standard substance into the standard working solution by using methanol; and (4) high performance liquid chromatography determining: carrying out sample introduction detection on the extract detection sample and the standard working solution by using high performance liquid chromatography to obtain a standard curve, and calculating the cannabinoid component content of the extract detection sample according to the standard curve by using high performance liquid chromatography data processing software. The method has the advantages of short peak appearance time, short detection time, high detection efficiency and accurate and stable detection result, and is suitable for industrial detection.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural phytochemical components, and in particular relates to a method for detecting cannabinoids in industrial hemp flowers and leaves and extracts thereof by high performance liquid chromatography. Background technique [0002] Industrial hemp refers to hemp that has been legally grown and contains less than 0.3% tetrahydrocannabinol (THC). At present, it has been legally grown and processed in Yunnan and Heilongjiang. In 2019, industrial hemp has become the hottest investment field, and it is also an emerging hot topic in the big health industry. The flowers and leaves of industrial hemp contain cannabinoids and non-cannabinoids, which are widely used in the field of medicine. Among them, the cannabinoids are mainly divided into tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabidiol (CBG), cannabidiol (CBDV), cannabichromene (CBC) , THCV and dronabi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/146
Inventor 尹升权唐瑛高建慎杨成周张岚燕
Owner 滇麻生物科技(曲靖)有限公司
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