112results about How to "Solve key problems" patented technology

The invention relates to a microfluidic cell array chip for high-throughput medicament screening, a preparation method thereof and use thereof. The microfluidic cell array chip for high-throughput medicament screening is characterized by sequentially comprising a valve-controlled channel layer, a fluid channel layer and a glass layer suitable for the anchorage growth of cells from top down. The chip can be used in high-throughput medicament screening by the parallel action of different medicament concentrations on various cells. The chip is manufactured by a multixposure one-development Su-8 negative photoresist process and multilayer PDMS bonding and has a structure with a high depth-to-width ratio. The microfluidic cell array chip provided by the invention can realize low reagent consumption and high-throughput co-culture of various cells, parallel analysis of the stimulation effect of different medicament concentrations on different cells and the real-time observation and detection of a slide of the cells and provide a fire-new technical platform and a method for high-throughput medicament screening and cell-medicament researches.

The invention discloses a priori shape modeling method based on a combined sparse model, and belongs to the technical field of medical image segmentation. Through priori shape modeling, a shape library obtained by collecting clinical data is established in allusion to a specific tissue organ, wherein the shape library consists of segmented shapes of image data from different patients, so as to establish a golden standard for corresponding organs of the patients. The priori shape modeling method based on a combined sparse model comprises the following steps: 1, gridding the surfaces of the shapes in advance by sampling points on the golden standard surfaces, wherein gridded shapes in the shape library are taken as training data of a model; 2, representing the gridded shapes by a sparse shape combining model, wherein coordinates of each shape in the shape library corresponding to all vertexes of a grid are arrayed as a column vector and an array D is obtained from the whole shape library; 3, performing optimization algorithm on the sparse shape combining model to obtain a corresponding parameter; and 4, performing inverse transformation on the corresponding parameter obtained in the optimization algorithm to obtain a required priori shape.

The invention discloses a design method of a clearing transaction separate accounting algorithm for a blockchain, comprising the following steps: S1, disassembling a three-party transaction into a plurality of atom transactions according to a user account and an account, and packaging the original three-party transaction and the atom transactions into composite transactions; S2, packaging the multiple composite transactions for the consensus to form a block, and then grouping the atom transactions in the block according to the user account and sorting the atom transactions in the block in chronological order; S3, for the non-quantized information, obtaining the last related transaction, and updating it to the final state value; S4, for the quantitative information, combining the transactions grouped by the user account according to the fund and the asset account, and calculating an intermediate value of the account change; and S5, querying the balance of the user account, and performing calculation with the intermediate value of the account change to get the final account balance. The design method has the beneficial effects of providing a simple and efficient transaction separateaccounting method suitable for the blockchain clearing platform.

The invention provides an ultra wide band plasma filter provided with an artificial surface. The ultra wide band plasma filter comprises a coaxial waveguide of which the two ends are symmetrical, a transitional waveguide from the coaxial waveguide to a cylindrical plasma waveguide, and the intermediate cylindrical plasma waveguide, wherein the transitional waveguide comprises an inner conductor transition and an outer conductor transition; the inner conductor transition is realized through a periodic ring-shaped groove array of which the radius and the depth change simultaneously; the outer conductor transition is realized through a horn antenna of which the opening changes gradually as a curve changes; the cylindrical plasma waveguide consists of the periodic ring-shaped groove array of which the radius and the depth are constant. The ultra wide band plasma filter provided by the invention has an open symmetrical structure, the compact size, the wide band, a high performance and a simple structure, is especially suitable for being used with a traditional microwave or a terahertz transmission line in a matching manner, can be used as the novel wide band plasma filter, and provides a fire-new thought and scheme for the design and the application of the filter.

The invention relates to a vehicle-mounted bad weather real-time sensing and collision pre-warning system, which comprises a machine vision perception module A, a collision pre-warning module B and a vehicle-vehicle environment sensing module C, wherein the module A is used for processing video information collected by a vehicle-mounted CCD camera by adopting an embedded microcomputer system and identifying real-time weather Information of a road segment on which the vehicle runs; the module B is used for fusing vehicle motion parameter information and traffic weather information, evaluating collision danger information and carrying out pre-warning intervention; and the module C is used for building an intra-regional traffic safety system by using a multi-vehicle communication technology and an intelligent decision making technology. The invention provides a traffic safety solution which can play a positive role in a traffic environment with a relatively poor weather condition, has relatively high practicability and is efficient and stable; improvement of the active safety performance of the vehicle in the bad weather condition is facilitated; the overall risk factor of the regional traffic system is effectively reduced through the multi-vehicle communication technology and the intelligent decision making technology; and the traffic safety is ensured.

The invention relates to a cold-rolled plate shape control method based on an optimization algorithm. The cold-rolled plate shape control method based on the optimization algorithm is characterized by comprising the following steps of: sequencing each plate shape adjusting mechanism in an L1-level base automated central processing unit (CPU) according to the sequence of a supporting roller inclining mechanism, an intermediate roller bending mechanism, a working roller bending mechanism and an intermediate roller transversely moving mechanism; invoking a formula (2) and a formula (3) derived from an evaluation function F for each plate shape adjusting mechanism to calculate the action adjusting value of each plate shape adjusting mechanism; and after calculation is finished, transmitting all the action adjusting values to each plate shape adjusting mechanism by using the CPU to control the plate shape so as to eliminate the plate shape deviation of each plate shape adjusting mechanism. By the cold-rolled plate shape control method based on the optimization algorithm, the key problems in the conventional cold-rolled plate shape control system can be solved, and the method can be directly applied to the plate shape control procedure of the L1-level base automated CPU, so that the advantage in the aspect of improving the calculation precision on the action adjusting quantity of the plate shape control mechanism is exerted completely.

The invention discloses a repeating coil design method for a three coil structure middle distance wireless energy transmission system, comprising steps of setting a transmission distance for the middle distance wireless energy transmission system, calculating load power and integral efficiency of the three coil type wireless energy transmission system containing the repeating coil and maximized power or the impedance condition when the efficiency is output, establishing an efficacy integration target function with the practical power and efficiency need as optimization goals, determining a restriction condition of the middle distance wireless energy transmission system according to a practical application index, and finally determining the repeating coil optimization parameters when the optimization goal is achieved.

The invention relates to a welding technology in the equipment manufacture industry and relates a special welding technology of an MCL (Mean Control Limit) type horizontally-split centrifugal compressor welder casing, in particular to a welding technology of a megaton ethane centrifugal compressor welder casing with forgeable pieces of 16 Mn and Q345R and ZQ230-450 materials. Gas shielded welding is adopted to weld the welder casing with the forgeable pieces of 16 and the Q345R low alloyed steel, and an electrode in a brand of H08Mn2SiS with a diameter shown in the specification, power supply polarity of DCRP (Direct Current Reverse Polarity) is adopted and comprises fore-welding treatment, welding process and post-welding treatment, wherein the welding process comprises the following process parameters: a welding current is 250-280 A, a welding voltage is 28-32 V, a protective mixed gas comprises 80 percent by volume of Ar and 20 percent by volume of CO2, a welding speed is 280-320 mm/min, the dry extension is 20-25 mm, and the gas flow is 15-20 L/min. The invention can solve the problems of welding deformation, layered tearing, low welding efficiency, and the like and ensure the welding seam strength by selecting an MAG (Metal Active Gas) shielded welding material with similar chemical contents and matched strength with a welder casing material.

The invention discloses a prefabricated and assembly type steel reinforced concrete column with a high-performance concrete frame. The prefabricated and assembly type steel reinforced concrete column adopts an idea of partial prefabricated assembly and partial casting in situ and comprises a prefabricated part and a cast-in-situ part. The prefabricated assembly type steel reinforced concrete column comprises a prefabricated high-performance concrete hollow column, longitudinal bars, section steel, stirrups and cast-in-situ common concrete, wherein the longitudinal bars are arranged in the prefabricated high-performance concrete hollow column along the column-length direction; the section steel is arranged in the prefabricated high-performance concrete hollow column along the column-length direction; the stirrups are spread on the cross section of the prefabricated high-performance concrete hollow column at equal intervals; the longitudinal bars and the section steel are enclosed by the stirrups; a central cavity of the prefabricated high-performance concrete hollow column is filled with the cast-in-situ common concrete. The invention also provides a construction method. The prefabricated and assembly type steel reinforced concrete column with a high-performance concrete frame and the construction method disclosed by the invention have the advantages that the hollow prefabricated high-performance concrete column can generate circumferential constraining force for the cast-in-situ common concrete, so that the bearing capability is improved, the whole performance and the seismic resistance of the structure are improved, the deformation capability of a member in the process of transportation and lifting is improved, and the comprehensive improvement and enhancement of the stressing performance and the use performance of the prefabricated steel reinforced concrete member are realized.

The invention discloses a research for realizing quick classification and identification of chemical components in an ixeris sonchifolia hance injection based on a UPLC-Q-TOF-MS technology, and aims to take flavonoids, organic acids, amino acids and nucleosides in the ixeris sonchifolia hance injection as research objects to realize quick classification and identification of the chemical components in the ixeris sonchifolia hance injection based on a UPLC-Q-TOF-MS technical platform. The research comprises the following steps: firstly, performing information integration on components of flavonoids, organic acids, amino acids and nucleosides in the ixeris sonchifolia hance injection to discover and summarize a rule for diagnosing fragments and neutral losses of the four types of substances; meanwhile, performing mass spectrographic analysis on reference substances of different types of compounds by adopting the UPLC-Q-TOF-MS technology, and performing verification; and constructing a method for realizing quick classification and identification of chemical components in the ixeris sonchifolia hance injection by using a method for diagnosing fragments and neutral losses as a screening and identifying tool.

The invention discloses a marine bacillus polypeptide and a preparation and an application thereof and belongs to the technical field of marine microorganism medicines. The marine bacillus polypeptide is from metabolites of antarctic marine bacillus N11-8 subjected to fermentation cultivation; 15 amino acid sequences at N-ends of the active polypeptide are ASTGSQKVTVYAVAD; the active polypeptide has relatively high cytotoxicity to a plurality of tumor cells such as human hepatoma carcinoma cell BEL-7402, human ovarian carcinoma cells NIH:OVCAR-3, human renal clear cell carcinoma cells 786-0 and human large cell lung cancer cells NCI-H460, and has relatively good research and application value. The polypeptide can be applied to medicines for preventing and/or treating the cancers.

The invention discloses an inversion method of an airport pavement medium layer electromagnetic property based on a system identification theory. The invention mainly relates to estimation of an airport pavement structure layer dielectric constant, conductivity and a thickness. The method is characterized by: firstly establishing a transmission model transmitted by an electromagnetic wave in the pavement structure layer, wherein an influence of each frequency component in an emission bandwidth on a medium-layer dielectric-constant imaginary part is considered in the transmission model; simultaneously introducing super-resolution parameter estimation WRELAX algorithm to estimate time delay generated when a radar receives an echo signal so that the inversion of the airport pavement structure layer electromagnetic property can be realized based on the system identification theory. In the invention, the estimation of the pavement medium layer dielectric constant, the conductivity and the thickness in the airport pavement structure which contains a levelling thin layer and does not contain the levelling thin layer can be realized respectively. Simultaneously, estimation precision of the medium layer dielectric constant can be increased and a key problem in airport pavement detection can be solved. Therefore, the method has important significance for guaranteeing airport safety operation.

The invention discloses a RTK positioning method of a cellular network. The method comprises the following steps: selecting a fixed reference station, setting a standard coordinate, acquiring observation data and sending to a centralized control center, wherein the centralized control center distributes a reference station scheduling center corresponding to the fixed reference station; the fixed reference station computes pseudo-range residual and carrier phase residual and sends the same to the reference station scheduling center; a mobile station coarsely positions itself and transmits the positioning result to the centralized control center; the centralized control center distributes the reference station scheduling center corresponding to the mobile station; the reference station scheduling center matches the reference station corresponding to the mobile station, and sends the matched reference station to the mobile station; the mobile station receives the reference station data; and the mobile station positions in high precision. The invention further provides a system for positioning by use of the position method. The system comprises the centralized control center, the plurality of reference station scheduling centers, the fixed reference station, the mobile station and the communication link. The positioning method disclosed by the invention is fast in positioning speed, high in precision, low in cost and small in data processing pressure.

The invention discloses a two-freedom-degree hybrid stepping motor for bionic eyeballs of a robot. The stepping motor comprises a first freedom degree motor, a second freedom degree motor and output shafts, wherein the first freedom degree motor is of a circular structure with a notch, the second freedom degree motor is of a circular structure with an opening, the notch is matched with the opening, and the planes of the two motors are perpendicularly orthogonal; the first freedom degree motor and the second freedom degree motor both comprise stators and rotors, the stators and the rotors can relatively move in the planes of the motors, and the two-freedom-degree movement of the output shaft of one freedom degree motor can be achieved through respective independent movement of the two freedom degree motors. The two-freedom-degree hybrid stepping motor has the advantages of being simple in structure and manufacture technology, convenient in analysis design, high in mechanical integration level, high in open-loop operation location accuracy, easy in performance experiment test and favorable for shortening the development period of products and improving the performance and the competitiveness of the products.

The invention relates to a system for extracting deep intercrystalline brine in a salt lake mining area. The system comprises an air compressor which is arranged on a salt lake mining layer, and an air-injection well, brine collecting wells and a brine conveying channel, which are formed in the salt lake mining layer in a digging manner, wherein the air compressor is connected with a high-pressure air pipe by an air reservoir; the high-pressure air pipe extends into the air-injection well; a brine tail liquid injection pipeline is arranged in the air-injection well; the brine collecting wells are distributed around the air-injection well; a brine conveying pipeline is arranged in each brine collecting well; a brine collecting pump is arranged in each brine collecting well and is connected with one end of the corresponding brine conveying pipeline; the other end of each brine conveying pipeline is connected with the brine conveying channel; sealing materials are arranged between the high-pressure air pipe and the brine tail liquid injection pipeline, between the high-pressure air pipe and the wall of the air-injection well, and between the brine tail liquid injection pipeline and the wall of the air-injection well respectively. According to the system, intercrystalline brine and deep brine can be pressed out by using compressed air, and simultaneously tail liquid after potassium extraction can be used for resolving low-grade solid potassium or high-grade solid potassium after dilution, so as to realize the aim of fully utilizing resources.

An object clustering method in situation assessment includes the steps: firstly, generating a property priority list and a clustering factor matrix; secondly, segmenting objects in a battlefield into clusters according to the first priority selected from the property priority list and deleting the selected priority from the property priority list; thirdly, judging whether the object quantity in the clusters is greater than the preset threshold value of the object quantity or not, if so, then continuously selecting a priority from the property priority list to segment the clusters, if not or the property priority list is empty, then performing clustering analysis on object data in the bottom clusters to generate mini-clusters according to the object location relationship; and finally, utilizing a fusion algorithm to fuse the mini-clusters, and then acquiring an object group. The object clustering method in the situation assessment overcomes defects in the object clustering and solves the key problem that accuracy and speed of object clustering are low, so that the battlefield situation assessment and policies made by the commander in the battlefield can be effectively supported.

The present invention is the preparation process of mercapto carbon nanotube, relates to material physics and chemistry, and solves the technological problem of compatibility and binding force between carbon nanotube and metal material. The main technological path includes the successive steps of: treating carbon nanotube with mixed acid of concentrated nitric acid and concentrated sulfuric acid for grafting carboxyl group and hydroxyl group, reducing the grafted carboxyl group into hydroxyl group with LiAlH4, reacting with halogen containing reagent to convert the hydroxyl group on carbon nanotube into halogeno radical, and final reacting the carbon nanotube with halogeno radical and hydrosulfide to obtain the mercapto radical modified carbon nanotube. The mercapto radical modified carbon nanotube is used mainly for preparing composite metal material and functional material.

The invention provides a method for preparing a high-conductivity aluminum-based composite material by utilizing low-resistivity carbon nanotubes. The steps are: take the original carbon nanotubes for pretreatment process; then coat the surface with a layer of material that can effectively block the aggregation of carbon nanotubes and improve the wettability of carbon nanotubes and aluminum liquid to obtain a composite powder; The powder is added to the molten aluminum for vacuum melting, so that the carbon nanotubes can be evenly distributed in the aluminum matrix, and the target product can be obtained after casting. The method of the invention can reduce the bulk resistivity of the original high-resistivity carbon nanotubes, increase the wetting performance between the carbon nanotubes and the aluminum liquid, improve the dispersion performance of the carbon nanotubes in the aluminum matrix, and improve the performance of the aluminum matrix. Conductivity of composite materials.