The invention provides a
chemiluminescence immunoassay device which comprises a sample supply mechanism, a
chemical reaction incubation mechanism, a sample adding mechanism and a
luminescence detection mechanism, wherein the sample supply mechanism comprises a plurality of sample tubes, a sample disc, a plurality of
reagent tubes and a
reagent disc, wherein the sample disc and the
reagent disc are respectively in a circular ring shape and can rotate. The
chemical reaction incubation mechanism is set to be at constant temperature, and comprises a rotary table, a plurality of reaction discs, a plurality of reaction cups and a driving mechanism, wherein the rotary table is in a circular shape; the plurality of reaction discs are arranged on the rotary table at equal intervals along the circumferential direction of the rotary table; reaction cup containing cavities are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the plurality of reaction cups are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the driving mechanism is connected with the rotary table and the plurality of reaction discs by power, so that the rotary table can rotate, and the reaction discs are driven to make revolution or respectively rotate. The sample adding mechanism comprises a liner movement unit, a sample needle, a reagent needle and a cleaning groove. The
luminescence detection mechanism comprises a
cleaning needle, a substrate needle, a light insulation cover and a detection head.