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Chemiluminescence immunoassay device

A technology of chemiluminescence immunoassay and analysis device, which is applied in the field of chemiluminescence immunoassay device, which can solve the problems of large size, low flux and low efficiency of the analyzer, and achieve the effects of increasing detection flux, reducing space and improving work efficiency

Inactive Publication Date: 2015-07-15
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, some of the existing domestic chemiluminescent immunoassay analyzers use rail-type feeding mechanisms, resulting in a larger size of the analyzer
The reagents in the cuvette are mixed evenly by rack and pinion transmission, and there is a lot of noise
And low efficiency, low flux

Method used

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Embodiment Construction

[0054] The chemiluminescent immunoassay device according to the present invention will be described in detail below with reference to the accompanying drawings.

[0055] refer to Figure 1 to Figure 5 , the chemiluminescent immunoassay device according to the present invention includes a sample feeding mechanism 1 , a chemical reaction incubation mechanism 2 , a sample adding mechanism 3 and a luminescence detection mechanism 4 . The sample supply mechanism 1 includes: a plurality of sample tubes 11, each sample tube 11 is used to contain samples to be tested; a sample tray 12 is circular and rotatable, and is provided with accommodating sample tubes 11 along the circumferential direction of the sample tray 12 a plurality of reagent tubes 13, and each reagent tube 13 is used to hold reagents; the reagent plate 14 is circular and rotatable, and is concentrically arranged on the inner side of the sample plate 12, and is arranged along the circumference to accommodate reagents. ...

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Abstract

The invention provides a chemiluminescence immunoassay device which comprises a sample supply mechanism, a chemical reaction incubation mechanism, a sample adding mechanism and a luminescence detection mechanism, wherein the sample supply mechanism comprises a plurality of sample tubes, a sample disc, a plurality of reagent tubes and a reagent disc, wherein the sample disc and the reagent disc are respectively in a circular ring shape and can rotate. The chemical reaction incubation mechanism is set to be at constant temperature, and comprises a rotary table, a plurality of reaction discs, a plurality of reaction cups and a driving mechanism, wherein the rotary table is in a circular shape; the plurality of reaction discs are arranged on the rotary table at equal intervals along the circumferential direction of the rotary table; reaction cup containing cavities are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the plurality of reaction cups are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the driving mechanism is connected with the rotary table and the plurality of reaction discs by power, so that the rotary table can rotate, and the reaction discs are driven to make revolution or respectively rotate. The sample adding mechanism comprises a liner movement unit, a sample needle, a reagent needle and a cleaning groove. The luminescence detection mechanism comprises a cleaning needle, a substrate needle, a light insulation cover and a detection head.

Description

technical field [0001] The invention relates to a chemiluminescent immunological detection system, in particular to a chemiluminescent immunoassay device. Background technique [0002] Chemiluminescence immunoassay (CLIA) is a newest immunoassay technique developed after radioimmunoassay, enzyme immunoassay, and fluorescence immunoassay. Chemiluminescence immunoassay consists of immunoassay and chemiluminescence assay. The immunoassay system uses chemiluminescent substances or enzymes as markers, directly marks on antigens or antibodies, and reacts with antigens to form antigen-antibody immune complexes. The chemiluminescent system is a luminescent substrate that is added with an oxidant or enzyme after the immune reaction is over. After the chemiluminescent substance is oxidized by the oxidant, an intermediate in an excited state is formed, which emits photons and releases energy to return to a stable ground state. The luminescence signal measuring instrument can be used ...

Claims

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Application Information

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IPC IPC(8): G01N35/02
CPCG01N35/025
Inventor 杨东超刘平叶翀王峤袁振清吴小胜罗晓琴
Owner TSINGHUA UNIV
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