The invention discloses an Ausferrite ductile cast iron grinding ball, and relates to a spherulitic graphite-contained cast iron alloy. The Ausferrite ductile cast iron grinding ball comprises the following chemical elements in percentage by mass: 3.3-3.7% of C, 2.0-3.0% of Si, 1.0-2.5% of Mn, 0.7-3.0% of Cr, 0.1-1.0% of Mo, 0.1-1.0% of Cu, 0.04-0.08% of P, 0.01-0.020% of S, 0.03-0.05% of Mg, 0.02-0.04% of Ce, 0.03-0.07% of B, 0.06-0.12% of Ti, 0.03-0.2% of V, and the balance of Fe. The Ausferrite ductile cast iron grinding ball is prepared by the steps of preparation and smelting of raw materials, spheroidizing treatment, inoculation treatment and microalloying treatment, and isothermal quenching heat treatment. The Ausferrite ductile cast iron grinding ball overcomes the defects of low production efficiency and high consumption of electric energy of a ball mill due to easy surface stripping and crushing and shorter fatigue life of an existing grinding ball product in the service process.