325 results about "Silica column" patented technology

The invention discloses a preparation method of four new skeleton alkaloid compounds with anti-inflammatory effect in the root of Anachymin and the use of the anti-inflammatory activity thereof. The four new skeleton alkaloid compounds are extracted from the root (root of Anachymin) of Roman pyrethrum (Anacyclus pyrethrum (L.) DC.) by using an organic solvent, and then separated by two or three methods of normal phase silica gel column chromatography, reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain eight new skeleton alkaloid monomer compounds, and the structures thereof are identified by high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy and X-ray single crystal diffraction. The invention also provides the NO inhibition effect of these compounds.

The invention discloses a polycyclic polyisopentenyl phloroglucinol compound as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The method comprises the following steps: by taking a dry overground part of hypericum monogynum as a raw material, performing percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and performing extraction and concentration to obtain a petroleum ether part extract; the extract is taken and subjected to separation through a positive and negative phase silica gel column, MCI medium-pressure column chromatography and Sephadex LH-20 gel column chromatography and purification through high performance liquid chromatography, the polycyclic polyisopentenyl phloroglucinol compound is prepared, and the structure and configuration of the compound are determined by means of a spectrum analysis technology, quantum chemistry calculation, X-ray single crystal diffraction and the like. The compound can inhibit TNF-alpha protein to play an immunosuppressive role, and can be used as an immunosuppressive drug.

The invention relates to the technical field of ferula asafoetida endophytic fungi separation and purification, in particular to alkaloid compounds in ferula asafoetida endophytic fungi as well as a preparation method and application of the alkaloid compounds in the ferula asafoetida endophytic fungi. And extracting with dichloromethane to obtain dichloromethane part extract, and purifying and separating the dichloromethane part extract by adopting silica gel column chromatography and high performance liquid chromatography to obtain the alkaloid compounds in the endophytic fungi of ferula sinkiangensis. The alkaloid compound in the ferula sinkiangensis endophytic fungi is disclosed for the first time, the compound is subjected to an in-vitro anti-tumor pharmacodynamic experiment, and according to the experiment result, the alkaloid compound has a certain inhibition effect on MCF-7 cells; therefore, the alkaloid compound can be used for preparing the medicine for preventing and treating the breast cancer, and a new medicine is provided for preventing and treating the breast cancer.

The invention relates to the technical field of ferula asafetida endophytic fungus strain separation and purification, in particular to an alkaloid component in pleurotus nebrodensis as well as a preparation method and application thereof, and the alkaloid component in pleurotus nebrodensis, namely an alkaloid compound in pleurotus nebrodensis, is prepared by the following steps: crushing pleurotus nebrodensis sporocarp, adding methanol, heating, refluxing and extracting; dispersing the pleurotus nebrodensis total extract into a suspension with water, and extracting to obtain a petroleum ether part and ethyl acetate part extract; and carrying out gradient elution on the ethyl acetate part extract by silica gel column chromatography, and separating and purifying the third fraction by high performance liquid chromatography to obtain the alkaloid compounds in pleurotus nebrodensis. The invention discloses the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C for the first time, and the pleurotus nebrodensis alkali C has a certain inhibition effect on HGC-27 cells, so that the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C, can be applied to the preparation of tumor prevention drugs and antitumor drugs.

The present invention provides a kind of detection method of thirteen kinds of related substance contents in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the chromatographic conditions are: the test solution and the reference solution are injected into the high performance liquid chromatograph, and the chromatographic conditions are: the chromatographic column adopts octadecyl bonded silica gel column; the ultraviolet detector is used, and the detection wavelength is 260nm; the injection volume: 10μl; the column temperature: 30℃‑40℃; the flow rate: 0.8ml / min‑1.0ml / min; the gradient elution method is used, and the mobile phase A: the solution of 0.02mol / L potassium dihydrogen phosphate solution adjusted to pH 8.0 with potassium hydroxide solution and isopropanol mixed with a volume ratio of 95:5; the mobile phase B: the mixed solution of acetonitrile and isopropanol with a volume ratio of 95:5. The method can be used to detect the contents of 13 kinds of related substances in vonoprazan fumarate, and high performance liquid chromatography is used for detection, and the method has high detection sensitivity, and the verification results show that the system applicability and specificity of this method meet the requirements, and the needs of drug production quality monitoring can be met.

The invention relates to the technical field of indole alkaloid compounds, in particular to indole pleurotus nebrodensis alkali A, a preparation method thereof and application of the indole pleurotus nebrodensis alkali A to preparation of drugs for preventing and resisting tumors. Pleurotus nebrodensis sporocarp is smashed, ethyl alcohol is added, heating reflux preparation and concentration are conducted, obtained pleurotus nebrodensis total extract is dispersed into suspension with water, and then the suspension is placed in a drying oven to be dried to obtain the indole pleurotus nebrodensis alkali A. The indole pleurotus nebrodensis alkali A is obtained. The method comprises the following steps: sequentially extracting with petroleum ether, dichloromethane and ethyl acetate, concentrating an extracting solution to obtain ethyl acetate part extract, carrying out gradient elution separation on the ethyl acetate part extract by silica gel column chromatography, carrying out gradient elution on the obtained third fraction by high performance liquid chromatography, purifying and separating, and collecting eluate to obtain the indole pleurotus nebrodensis alkali A at 16.3 minutes. The indole pleurotus nebrodensis alkali A is disclosed for the first time, and it is found through in-vitro antitumor pharmacodynamic experiments that the indole pleurotus nebrodensis alkali A has a certain inhibition effect on human HGC-27 gastric cancer cells, so that the indole pleurotus nebrodensis alkali A can be used for preparing drugs for preventing and resisting gastric cancer.

The invention relates to the technical field of photo-thermal therapy, in particular to a monomolecular photo-thermal agent with high photo-thermal conversion efficiency and a preparation method and application of the monomolecular photo-thermal agent. The preparation method comprises the following steps: S1, synthesis of a compound X1: under the protection of argon, dissolving 2, 4-dimethyl pyrrole in dichloromethane, stirring in an ice bath, adding trifluoroacetic anhydride, heating to room temperature after stirring, extracting after quenching reaction, combining organic phases, drying, filtering, concentrating under reduced pressure and purifying by silica gel column chromatography to obtain the compound X1. The prepared monomolecular photothermal agent with the high photothermal conversion efficiency is high in water solubility, has the high photothermal conversion efficiency in an aqueous solution and is high in light stability, the immune escape phenomenon possibly caused by introduction of a high polymer material is avoided, and the technical problem that an existing photothermal agent is difficult to achieve the high photothermal conversion efficiency in the aqueous solution is solved.

The invention discloses a euphorbia kanane type triterpenoid compound as well as a preparation method and application thereof, and relates to the technical field of natural product chemistry, the compound has at least one structural formula as shown in the specification: # imgabs0 #; the preparation method comprises the following steps: by taking Xiangluxiang as a raw material, extracting by using a mixed solution containing methanol and ethyl acetate, removing insoluble impurities and lower brown oily substances, and concentrating filtrate under reduced pressure to obtain an extract; and separating the extract through normal-phase silica gel column chromatography and / or MCI column chromatography, reverse-phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain the compound. The invention also provides application of the compound in preparation of anti-inflammatory drugs. The preparation method has the beneficial effects that four euphorbia kanane type triterpenoids which are rich in content and separated from the champignon have no obvious cytotoxicity on RAW264.7 cells, can inhibit the generation of NO in the RAW264.7 cells to different degrees, and can be used for preparing anti-inflammatory drugs.

The invention belongs to the technical field of agricultural chemistry, and particularly relates to a naphthoquinone compound with tobacco black shank resisting activity as well as a preparation method and application of the naphthoquinone compound. The naphthoquinone compound is prepared by the following steps: by taking honeysuckle branches as raw materials, extracting with high-concentration methanol, high-concentration acetone / water or high-concentration ethanol / water, merging extracting solutions, filtering, and concentrating under reduced pressure to obtain extract; packing the extract by using a silica gel dry method and carrying out silica gel column chromatography; carrying out gradient elution by using a chloroform-acetone solution; and further separating and purifying the 7: 3 part of the eluent by high performance liquid chromatography to obtain the required naphthoquinone compound. The invention further discloses application of the compound, and activity tests show that the compound has a good inhibiting effect on tobacco black shank. The compound disclosed by the invention is novel in structure, has relatively good black shank resisting activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The present invention relates to a method for preparing a highly conjugated tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid compound having a unique 8,14,18,24-tetraazaoctacyclic [21.2.21,4.1.02,21.03,18.05,17.09,16.011,15] nonacosane ring system, and the anti-inflammatory and analgesic use of the compound. With regard to the tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid compound, the root of Anacyclus pyrethrum (L.) DC is extracted using an organic solvent, the resulting extract is extracted, separation is then performed by means of two to three methods of normal-phase silica-gel column chromatography, reversed-phase silica-gel column chromatography and semi-preparative high-performance liquid chromatography to obtain four tetraamino 6 / 6 / 6 / 5 / 7 / 5 octacyclic alkaloid monomer compounds having a new skeleton, and the structures of the compounds are identified by means of methods such as high-resolution mass spectrometry, and nuclear magnetic resonance spectroscopy. Further provided in the present invention are the Nav1.2 inhibitory effect and NO inhibitory effect of the compounds.

The invention belongs to the technical field of phytochemistry, and particularly relates to a benzo [e] pyrrolo [1, 2-a] aza # imgabs0 # compound as well as a preparation method and application thereof. The preparation method comprises the following steps: fermenting cigar stem barks, drying the fermented cigar stem barks in the sun, crushing and sieving to obtain a material a; adding a first solvent into the material a for reflux extraction to obtain an extracting solution, concentrating the extracting solution, adding a second solvent for extraction, combining the extracted extraction phases, and performing vacuum concentration to obtain an extract b; carrying out silica gel column chromatography on the extract b, eluting with chloroform-acetone eluent, monitoring by TLC (Thin Layer Chromatography), and collecting the eluent; and purifying the eluent by using a sephadex column, and separating and purifying by using high performance liquid chromatography to obtain the benzo [e] pyrrolo [1, 2-a] aza # imgabs 1 # compound with tobacco black shank resisting activity. The compound is novel in structure, has relatively good black shank resistance activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The invention provides a method for extracting raspberry ketone through raspberry enzymolysis and ultrasonic wave, and belongs to the technical field of natural product extraction. The method comprises the following steps: selecting cyan raspberry fruits which are about to be ripe and free from diseases and insect pests, washing, freeze-drying and crushing to 30 meshes; then, carrying out composite enzymolysis, and carrying out enzymolysis on cellulase, pectinase and hemicellulase at the mass ratio of 0.8%-1.2% to 3: 1: 2 for 2-4 hours under the conditions that the temperature is 50 DEG C and the pH is 5.0; and extracting for 20-60 minutes by using a 80% ethanol solution under the assistance of the ultrasonic power of 220W and the frequency of 30kHz. And finally, carrying out vacuum concentration and silica gel column chromatography separation to obtain a high-purity product. The method obviously improves the extraction rate, has the advantages of environmental protection and economy, and meets the requirements of industrial production on raspberry ketone extraction.

The invention discloses a method for determining impurities in a desloratadine oral solution, the impurities comprise a desloratadine impurity 1, the determination method adopts a high performance liquid chromatography, and the detection conditions of the high performance liquid chromatography are as follows: a chromatographic column is an octyl silane bonded silica gel column or an efficiency equivalent column; the detection wavelength is 254 + / -2nm; the detector is an ultraviolet detector; the column temperature is 35-45 DEG C; the flow rate is 1.1 to 1.3 mL / min; mobile phases: a dipotassium phosphate buffer solution, methanol and acetonitrile; the elution mode is isocratic elution. The determination method disclosed by the invention is good in specificity, good in separation degree, high in sensitivity and good in accuracy, can fully meet the requirements of impurity determination, has important meanings for evaluating the quality of raw material medicines and the advantages and disadvantages of a preparation process, controlling the quality of a final product and detecting a stable sample, and is high in practicability in actual quality control work.

The invention discloses a double-ratio type optical detection probe and a preparation method and application thereof, and the preparation method comprises the following steps: dispersing 16, 17-dihydroxy anthrone violet and anhydrous potassium carbonate in N, N-dimethylformamide, stirring, rapidly adding 4-bromomethyl phenylboronic acid pinacol ester, heating to 120 DEG C, and reacting for 12 hours; slowly dropwise adding the reaction solution into methanol, settling for 24 hours, carrying out suction filtration, washing and extraction for three times, collecting an organic layer, carrying out rotary evaporation and drying, and purifying through silica gel column chromatography to obtain the anthrone violet derivative VTP; the preparation method comprises the following steps: uniformly mixing a tetrahydrofuran solution of an anthrone violet derivative VTP with a preset concentration and a tetrahydrofuran solution of an amphiphilic surfactant, injecting into water with a preset volume, and purifying to obtain the water-soluble optical probe. The invention solves the problems of low stability, poor specificity and high cost of most near-infrared fluorescent or photoacoustic organic probes, has good water solubility and biocompatibility, and can eliminate interference of other reactive oxygen free radicals in a normal physiological environment.

The invention relates to the technical field of biological medicines, in particular to a polycyclic polyisopentenyl cyclopentanone compound as well as a preparation method and application thereof. The polycyclic polyisopentenyl cyclopentanone compound provided by the invention has a structural formula as shown in a formula 1; the preparation method comprises the following steps: by taking dry fruits of hypericum berry as raw materials, carrying out percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and extracting and concentrating the suspension with petroleum ether to obtain a crude extract; the crude extract is taken and subjected to positive and negative phase silica gel column separation, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatographic separation, high performance liquid chromatography preparation and CHIRALPAK IG chiral column resolution, and the polycyclic polyisopentenyl cyclopentanone compound is obtained. The protection activity of the compound on AC16 myocardial cell injury induced by cold ischemia is evaluated, and the compound can be used for preparing the polycyclic polyisopentenyl cyclopentanone compound. The compounds can be used as lead compounds for developing medicines for preventing and treating myocardial cold ischemia injury.

The invention belongs to the technical field of natural pharmaceutical chemistry, and discloses a benzofuran compound in dalbergia odorifera and a preparation method and application thereof.The benzofuran compound is separated from dalbergia odorifera of a leguminous dalbergia plant, the heartwood of the dalbergia odorifera is extracted and concentrated to obtain extract, and the extract is filtered to obtain the benzofuran compound. The extract is taken and sequentially subjected to silica gel column chromatography, medium-pressure ODS column chromatography and gel column chromatography separation for multiple times, then semi-preparative high performance liquid chromatography is used for purification, and the benzofuran compounds can be obtained. According to the present invention, lipopolysaccharide (LPS) is adopted to induce RAW 264.7 cells to establish an inflammation model, such that the compound has good anti-inflammatory activity, IC50 is 14.33 [mu] M, and expression of inflammatory factor interleukin-6 (IL-6) and interleukin-1 [beta] (IL-1 [beta]) can be inhibited;

The application belongs to the technical field of natural medicinal chemistry, and discloses a benzofuran compound in Jinyunming and a preparation method and application thereof. The benzofuran compound is separated from Jinyunming of the plant Dalbergia hupeana Tutch in the family of Leguminosae. The heartwood of Jinyunming is extracted and concentrated to obtain extract, the extract is sequentially separated by multiple silica gel column chromatography, medium-pressure ODS column chromatography and gel column chromatography, and then purified by semi-preparative high-performance liquid chromatography, so that the benzofuran compound can be obtained. The application establishes an inflammation model by inducing RAW 264.7 cells with lipopolysaccharide (LPS), and shows that the compound has good anti-inflammatory activity, the IC50 is 14.33 μM, and the expression of inflammatory factors interleukin-6 (IL-6) and interleukin-1β (IL-1β) can be inhibited.

The invention discloses a capsaicin compound and a preparation method and application thereof, the molecular formula of capsaicin Capsivaryine is C16H21NO3, the chemical structure of capsaicin Capsivaryine is as follows, and the capsaicin Capsivaryine compound is a new compound reported for the first time and is prepared by taking mature and dry fruits of capsicum as raw materials through crushing, sieving, silica gel column treatment, D101 macroporous resin treatment and HPLC (High Performance Liquid Chromatography). The compound disclosed by the invention has a certain in-vitro inhibition effect on human breast cancer cells MCF-7, human liver cancer cells HEPG2, human non-small cell lung cancer cells A549 and human ovarian cancer cells SKOV3, and has no toxicity on human normal liver cells L02. The compound is a natural product which is novel in structure and good in activity, has great potential in the anti-tumor aspect, enriches diversity of pepper plants, and provides guidance for research of chemical components of pepper. # imgabs0 #

The invention belongs to the field of furniture, specifically a single-sided sticker furniture component of a mixed soft and hard wood multi-layer board. The four sides of the multi-layer wood board are sealed with silicone sealing strips, which play a buffering role when closed to increase its service life and reduce noise. A card slot is provided on the edge of the silicone sealing strip to facilitate the replacement of stickers. A slidable silicone column is provided between the silicone sealing strip and the multi-layer wood board, a pull ring is provided at one end of the side, and a handle is installed on the left and right edges of the multi-layer wood board to facilitate the opening of the cabinet door. A groove is provided on one side of the silicone column that is tightly fitted to the multi-layer wood board, so that the harmful gas formaldehyde emitted by the multi-layer wood board can fully contact with the groove, a chamber is provided on the front side of the silicone column, and the chamber and the groove are interconnected. Placing an activated carbon bag in the chamber can effectively adsorb free formaldehyde in the groove. After the activated carbon bag is saturated with adsorption for a period of time, the silicone column can be pulled out to replace the activated carbon bag.

The invention belongs to the technical field of natural pharmaceutical chemistry, and particularly relates to a novel iridoid compound, namely, uncarcinin, separated from wingceltis, a pharmaceutically acceptable isomer of the novel iridoid compound, a wingceltis extract containing the novel iridoid compound, and a preparation method and application of the novel iridoid compound. The chemical structure of the utazonine is shown as a formula I, and the absolute configuration of the utazonine is (1S, 3S, 3aR, 4R, 7aS). The invention also provides a method for extracting the compound or the extract from the stem of the wingceltis, and purifying by combining pH gradient extraction with silica gel column chromatography, semi-preparative high performance liquid chromatography and gel chromatography to obtain the compound or the extract. The wingceltis cycline, the isomer of the wingceltis cycline, the wingceltis extract containing the wingceltis cycline or the pharmaceutical composition show remarkable gastric mucosa protection activity in an in-vitro cell model, particularly, the protection rate of the wingceltis cycline to ethanol-induced human gastric mucosa epithelial cell (GES-1) injury at the concentration of 50 mu M can reach 5.21%, and the wingceltis cycline, the isomer of the wingceltis cycline, the wingceltis extract containing the wingceltis cycline or the pharmaceutical composition show definite gastric mucosa protection activity.

This invention relates to a novel substance with in vitro anti-inflammatory activity, its preparation method, and its application. The chemical name of maltol-3-1'-ethoxyhexane-1”,2”,3”,4”,5”,6”-hexaol-(4'-2”)-glucopyranoside is C. 21 H 34 O 13 Preparation method: 1. Extract *Trollius chinensis* with 70% ethanol (v / v); 2. Concentrate the extract until no alcohol odor remains and load it onto a D101 macroporous resin column; 3. Elute the fraction from the D101 macroporous resin with 30% ethanol, separate by silica gel column chromatography and gel column chromatography, and then obtain *Trollius chinensis* glycoside C using high-performance liquid chromatography. *Trollius chinensis* glycoside C is used to prepare anti-inflammatory drugs. The IC50 of *Trollius chinensis* glycoside C in this invention inhibits the release of pro-inflammatory factors TNF-α and IL-6 from LPS-stimulated mouse macrophages (RAW264.7). 50 The concentrations were 22.8 μmol / L and 31.0 μmol / L, respectively, indicating that it has good in vitro anti-inflammatory activity. This invention belongs to the field of research on effective components of natural drugs.

The invention discloses an alkaloid compound extracted from anacyclus pyrethrum (L.) DC. As well as an extraction method and application thereof. The alkaloid compound is extracted from the anacyclus pyrethrum (L.) DC. Root (L.) by adopting an organic solvent, and the extraction method comprises the following steps: extracting the alkaloid compound from the anacyclus pyrethrum (L.) DC. And separating by using two, three or four combined methods of normal-phase silica gel column chromatography, reverse-phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain the novel skeleton alkaloid compound. In addition, the compounds provided by the invention can play an anti-inflammatory role by inhibiting nitric oxide (NO) of cells, so that the compounds can be used for preparing anti-inflammatory drugs.

The present invention discloses a sesquiterpene glycoside compound and its preparation method and application. A new sesquiterpene glycoside ligulariatinside A is isolated and identified from the n-butanol extraction part of 95% ethanol extract from the root of Ligularia glossata by multiple chromatographic methods such as macroporous resin column chromatography, positive and reverse phase silica gel column chromatography and high performance liquid chromatography, as well as spectral methods. The molecular formula of the new sesquiterpene glycoside ligulariatinside A is determined to be C 21 H 34 O7, the structure is (3 S ,4 R ,5 R ,7 R )‑11‑hydroxy‑11,12‑dihydronootkatone‑11‑ O ‑ β ‑D‑glucopyranoside enriches the chemical diversity of Ligularia officinalis. Bacterial inhibition experiments revealed that the new sesquiterpenoid glycoside exhibits inhibitory activity against Vibrio anguillarum, with a minimum inhibitory concentration (MIC) of 50 μg / mL, laying the foundation for the development of new antibacterial drugs.

The invention discloses a high-yield method for reducing dinitro and retaining sensitive protecting groups, which comprises the following steps: adding water, sodium dithionite and sodium carbonate into a container, stirring and clarifying, and dropwise adding a mixed solution containing raw materials; after the reaction is finished, extracting, separating liquid, concentrating, and purifying by silica gel column chromatography to obtain a product and a byproduct; the raw material is a compound containing a carbon-carbon double bond, a p-methoxybenzyl protecting group, a t-butyloxycarboryl protecting group and dinitro. Adding a byproduct into a phosphoric acid mixed solution for reaction; and after the reaction is finished, adding dichloromethane and water, dropwise adding triethylamine to adjust the pH value, extracting, separating liquid, drying, concentrating, and purifying by silica gel column chromatography to obtain the product. According to the method, a sodium hydrosulfite / sodium carbonate system and phosphoric acid step-by-step treatment strategy is adopted, reduction of dinitro is achieved under mild conditions, meanwhile, PMB protecting groups, Boc protecting groups and C-C double bonds are reserved, and the problem that stability and high yield of the protecting groups cannot be considered at the same time in an existing method is solved.

The invention discloses a universal polymer force-sensitive cross-linking agent based on combination of a force-sensitive group and a bis-aziridine cross-linking function, and a preparation method and application thereof. The cross-linking agent is prepared by esterification of the force-sensitive group and a bis-aziridine group. The method comprises the following steps: in a nitrogen environment, carrying out a reaction on a force sensitive group, 4-[3-(trifluoromethyl) diazo-3-yl] benzoic acid, 4-dimethylaminopyridine (DMAP) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl) in anhydrous dichloromethane; and extracting the obtained mixture for three times, performing column chromatography purification on the obtained mixture on a silica gel column by using an eluent, and spin-drying to obtain a final product. The mechanical response sensitivity and structural stability of the material are remarkably improved, a mechanical force sensitive group introduction strategy suitable for various polymer systems is established, and a new technical path and theoretical support are provided for multifunctional design and wide application of the stress sensitive material.

The invention provides a novel probe for monitoring activity of glutamyltranspeptidase as well as a preparation method and application of the novel probe. The preparation method comprises the following steps: (S10) preparing a hemicyanine photoacoustic group containing two hydrophilic sulfonic groups and a hemicyanine molecular solution; (S20) dissolving N-Boc-Glu-OtBu and HATU in an organic solvent, adding DMAP, activating at 0-4 DEG C, adding the hemicyanine molecular solution prepared in the step (S10), and continuously stirring to react; (S30) removing unreacted raw materials and byproducts through washing and drying operations; and (S40) obtaining a target product GGT-probe through an acidic deprotection step and a silica gel column chromatography method, the target product GGT-probe has strong hydrophilicity, can monitor the activity of GGT in a living body level in a non-invasive manner by utilizing the photoacoustic property derived from hemicyanine, and is suitable for being applied to monitoring liver functions and systemic lipid metabolism diseases.

The present invention relates to a method for recovering manganese from ternary battery waste liquid. The method comprises the following steps: subjecting the ternary battery waste liquid to column chromatography treatment and collecting the eluate; subjecting the eluate to ion exchange treatment through a cation exchange resin and enriching the manganese ions in the eluate; wherein the chromatographic column filler used in the column chromatography treatment is a cross-linked aminosilane-modified silica microsphere. Through the reverse adsorption and elution effect of the aminosilane-modified silica microsphere, the impurity ions are adsorbed by the silica gel column and the manganese ions enter the eluate, thereby realizing the separation of manganese ions from impurity ions, and in conjunction with the subsequent cation exchange process, the manganese ions are adsorbed by the cation exchange resin to realize the enrichment of manganese ions. In addition, the method has a simple and easy-to-control process, is easy to operate, has low equipment investment, low reagent cost, mild reaction conditions, is green and environmentally friendly, has controllable quality and is easy to industrialize, has good environmental and economic effects, and has broad application prospects.

Provided in the present disclosure is a method for preparing a reference substance of antofine, which method comprises the steps of: soaking the Chinese herbal medicine Cynanchum komarovii Al. Iljinski in ethanol, extracting and filtering same, performing acid adjustment, extracting same with ethyl acetate, performing alkali adjustment, extracting same with chloroform, performing silica gel column chromatography, and then performing C18-column chromatography and precipitation, so as to obtain antofine with a purity of 99% or more. The extraction and separation efficiency is effectively improved by means of specific pre-treatments, and a target reference substance with high HPLC purity can be obtained with high throughput. In addition, the method for preparing a solid reference substance of antofine obtained in the present disclosure has very good industrial production applicability.

The invention discloses a method for detecting an initiator and a degradation product thereof in a polymer, a chromatographic column is an octadecyl bonded silica gel column, a gradient elution mode is adopted, and a detector is a diode array detector (DAD). The result shows that under the chromatographic condition, various impurities generated by degradation of the sevelamer carbonate catalyst can be well separated; the precision, repeatability and stability results are good. The method is simple and convenient to operate, high in repeatability and stable and reliable in result, and can be used for detecting the sevelamer carbonate initiator and the degradation product thereof.

The invention discloses a method for preparing lobster sarcosinine by using nereis as a raw material, and the lobster sarcosinine is prepared by adopting a combined process of reverse phase silica gel column chromatography, gel column chromatography, normal phase silica gel column chromatography and HPLC (High Performance Liquid Chromatography) preparation. Compared with a traditional lobster extraction method for preparing lobster sarcosinine, the method provided by the invention can realize high-purity separation of a target product; and the methanol solvent used in the process can be recycled through distillation, thereby conforming to the concept of green production. Therefore, deep development and efficient utilization of marine resources can be promoted, natural marine drug resource reserve is enriched, a new direction is provided for research and application in the fields of healthy development of human beings and disease prevention and control, and remarkable social benefits are achieved.