The invention belongs to the technical field of veterinary medicines, and particularly relates to a preparation method of Tylvalosin enteric coated granules. The preparation method comprises the following steps of (1) preparing Tylvalosin granules from: Tylvalosin, dextrin, orally-taken sugar, stevioside, ethyl maltol, a sausage casing material, a stuffing agent, a disintegrant and starch pulp; (2) performing material mixing: uniformly mixing the Tylvalosin with the dextrin, the orally-taken sugar, the stevioside, the ethyl maltol and the sausage casing material, performing crushing, performing screening to obtain mixed powder, adding an appropriate quantity of the solvent to the mixed powder, performing uniform mixing, adding the stuffing agent, the disintegrant and the starch pulp, and performing uniform mixing to prepare soft materials; and (3) performing granulation: making wet granule minipills from the prepared soft materials, and performing drying until the obtained Tylvalosin enteric coated granules are obtained. The prepared Tylvalosin enteric coated granules can be taken in groups, effective components of the Tylvalosin enteric coated granules are not destroyed by gastricacid and can safely reach intestines to be absorbed, and the feed conversion ratio can be notably increased.